Mechanical properties of bacterial hook filament

• Project/Area Number: 17340126
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics/Chemical physics
• Research Institution: Meiji University
• Principal Investigator: YOSHIMURA Hideyuki Meiji University, Department of Physics, Professor (70281441)
• Co-Investigator(Kenkyū-buntansha): NAKANO Takashi Tokyo Institute of Technology, Department of Mechanical and Aerospace Engineering, Assistant (00183517) ; MOMOZONO Satoshi Tokyo Institute of Technology, Department of Mechanical and Aerospace Engineering, Assistant (70262300) ; AIZAWA Shin-ichi Prefectural University of Hiroshima, Department of Life Science, Professor (50222451) ; TACHIKAWA Mki Meiji University, Department of Physics, Professor (60201612)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥16,490,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥690,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 2005: ¥11,400,000 (Direct Cost: ¥11,400,000)
• Keywords: flagella / polyhook / Young's modulus / rigidity / fluorescence / electronmicroscopy / rapid freezing / freeze dry / レーザトラップ

Research Abstract

Flagellated bacteria like Sal. Typhimurium swims by rotating helical flagella filaments with bundling and synchronizing them each other. Because axs of bacterial motors directed in different way, flexible joint part is required between motor and flagella filament. It is known that bacterial hook work as a universal joint of this part. However its mechanical feature like rigidity is not known exactly. Here we measured modulus of rigidity by using polyhook filament, length about 1μm, from mutant Sal. Typhimurium(SWJ108). Purified polyhook filaments were frozen rapidly in liquid ethane and freeze dried. Distribution of helix pitch and diameter were evaluated from electron micrographs of shadowed images. We got value of 95±23nm for helix pitch and 31±9.9nm for diameter of polyhook filament. We also measured fluctuation of helical pitch and diameter of flagella filament from negative stained samples. By comparing fluctuation of polyhook and flagella filaments, we have calculated the ratio of rigidity as G_<flagella>/G<hook>=120. According to the literature value of flagella filament rigidity, 3.8×10^9Pa, we obtained rigidity of polyhook as 3.3×10^7Pa. We also tried to measure the rigidity of flagella in solution directly using thin glass capillary. Fluorescent labeled flagella filament was fixed by anti-flagellin adsorbed on a glass capillary of diameter less than 1μm. Tension in pN range was measured from bending deformation of glass capillary under fluorescent microscope. We are now accumulating the results in this process. We are going to measure rigidity of super-poly hook which has length of few micro-meter by the same method.

Research Products

• [Journal Article] In Vitro synthesis of calcium nanoparticles using the protein cage of apoferritin (2008)
  • Author(s): H.Fukano, M.Aizawa, H.Yoshimura
  • Journal Title: Key Engineer.Mater. 361-363 Pages: 183-186
  • Description: 「研究成果報告書概要(和文)」より
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• [Journal Article] Microstructural observation of calcium-deficient single crystal apatite fibers and phase changes during heating (2008)
  • Author(s): A.Taki, H.Yoshimura and M.Aizawa
  • Journal Title: Key Engineer.Mater. 361-363 Pages: 147-150
  • Description: 「研究成果報告書概要(和文)」より
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• [Journal Article] In Vitro synthesis of calcium nanoparticles using the protein cage of apoferritin (2008)
  • Author(s): Fukano, H., Aizawa, M., Yoshimura, H.
  • Journal Title: Key Engineer. Mater. 361-363 Pages: 183-186
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Microstructural observation of calcium-deficient single crystal apatite fibers and phase changes during heating (2008)
  • Author(s): Taki, A., Yoshimura, H., Aizawa, M.
  • Journal Title: Key Engineer. Mater. 361-363 Pages: 147-150
  • Description: 「研究成果報告書概要(欧文)」より
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• [Journal Article] Production of single-walled carbon nanotubes with narrow diameter distribution using iron nanoparticles derivered from DNA-binding proteins from starved cells (2007)
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• [Journal Article] Gold-filled apo-ferritin for investigation of single-walled carbon nanotube growth on substrate (2007)
  • Author(s): D.Takagi, A.Yamazaki, Y.Otsuka, H.Yoshimura, Y.Kobayashi, Y.Homma
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• [Journal Article] Size Control of catalytic nanoparticles by thermal treatment and its application to diameter control of single-walled carbon nanotubes (2007)
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• [Journal Article] Systematic deletion analysis of the fla genes in the flagella operon identify several genes essential for proper assembly and function of flagella in the archaeon, Methanococcusmaripaludis (2007)
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• [Journal Article] The mechanism of outer membrane penetration by the eubacterial flagellum and implications for spirochete evolution (2007)
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• [Journal Article] The Two-Component Response Regulator RcsB Regulates Type 1 Piliation in Escherichia coli (2007)
  • Author(s): Schwan, W.R., Shibata, S., Aizawa, S.-I., and Wolfe, A.J.
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• [Journal Article] BifunctionalNanotube Scaffolds for Diverse Ligands Are Purified Simply from Escherichia coli Strains Coexpressing Two Functionalized Flagellar Genes (2007)
  • Author(s): Woods, R.D., Takahashi, N., Aslam, A., Pleass, R.J., Aizawa, S-I., and Sockett, R.E.
  • Journal Title: NanoLett. 7 Pages: 1809-1816
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• [Journal Article] BifunctionalNanotube Scaffolds for Diverse Ligands Are Purified Simply from Escherichia coli Strains Coexpressing Two Functionalized Flagellar Genes (2007)
  • Author(s): Mashimo, T., Hashimoto, M., Yamaguchi, S., and Aizawa, S.-I.
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• [Journal Article] T Characterization of two sets of sub-polar flagella in Bradyrhizobiumjaponicum (2007)
  • Author(s): Shibata, S., Takahashi, N., Chevance, F.F.V., Karlinsey, J.E., Hughes, K.T., and Aizawa, S.-I.
  • Journal Title: Mol Micro. 64 Pages: 1404-1415
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• [Journal Article] Characterization of two sets of sub-polar flagella in Bradyrhizobiumjaponicum (2007)
  • Author(s): Kanbe, M., Yagasaki, J., Zehner, S., Gottfert, M., Aizawa, S.-I.
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• [Journal Article] Flow-Assisted Optical Trapping of Microparticle Produced by Laser Ablation (2007)
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  • Journal Title: Jpn.J.Appl.Phys. 46
  • NAID: 40015671028
  • Description: 「研究成果報告書概要(和文)」より
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• [Journal Article] Size Control of catalytic nanoparticles by thermal treament and its application to diameter control of single-walled carbon nanotubes (2007)
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  • Journal Title: Appl. Phys. Lett. 90
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Production of single-walled carbon nanotubes with narrow diameter distribution using iron nanoparticlesderivered from DNA-binding proteins from starved cells (2007)
  • Author(s): Jeong, G. H., Yamazaki, A., Suzuki, S., H, Yoshimura, Y. Kobayashi, Y. Homma
  • Journal Title: Carbon 45 Pages: 978-983
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gold-filled apo-ferritin for investigation of single-walled carbon nanotube growth on substrate (2007)
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  • Journal Title: Chem. Phys. Lett. 445 Pages: 213-216
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Systematic deletion analysis of the fla genes in the flagella operon identify several genes essential for proper assembly and function of flagella in the archaeon, Methanococcusmaripaludis (2007)
  • Author(s): Chaban, B., Ng, S. Y. M., Kanbe, M., Saltzman, I., Nimmo, G., Aizawa, S.-I., Jarred, K. F.
  • Journal Title: Mol. Micro. 66 Pages: 596-609
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The mechanism of outer membrane penetration by the eubacterial flagellum and implications for spirochete evolution (2007)
  • Author(s): Chevance, F. F., Takahashi, N., Karlinsey, J. E., Gnerer, J., Hirano, T., Samudrala, R., Aizawa, S,-I, Hughes, K. T.
  • Journal Title: Genes. Dev. 21 Pages: 2326-2335
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The Two-Component Response Regulator RcsB Regulates Type 1 Piliation in Escherichia coli (2007)
  • Author(s): Schwan, W. R., Shibata, S., Aizawa, S.-I., Wolfe, A. J.
  • Journal Title: J. Bacteriol. 189 Pages: 7159-7163
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bifunctional Nanotube Scaffolds for Diverse Ligands Are Purified Simply from Escherichia coli Strains Coexpressing Two Functionalized Flagellar Genes (2007)
  • Author(s): Woods, R. D., Takahashi, N., Aslam, A., Pleass, R. J., Aizawa, S-I., Sockett, R. E.
  • Journal Title: NanoLett. 7 Pages: 1809-1816
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Temperature hyper-sensitive sites of the flagellar switch component FliG in Salmonella entericaserovarTyphimurium . (2007)
  • Author(s): Mashimo, T., Hashimoto, M., Yamaguchi, S., Aizawa, S.-I.
  • Journal Title: J. Bacteriol. 189 Pages: 5153-5160
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] FliK regulates flagellar hook length as an internal ruler (2007)
  • Author(s): Shibata, S., Takahashi, N., Chevance, F. F. V., Karlinsey, J. E., Hughes, K. T., Aizawa, S.-I.
  • Journal Title: Mol Micro. 64 Pages: 1404-1415
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterizatin of two sets of sub-polar flagella in Bradyrhizobiumjaponicum (2007)
  • Author(s): Kanbe, M., Yagasaki, J., Zehner, S., Gottfert, M., Aizawa, S.-I.
  • Journal Title: J. Bacteriol. 189 Pages: 1083-1089
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Flow-Assisted Optical Trapping of Microparticle Produced by Laser Ablation (2007)
  • Author(s): Ochiai, T., Numoto, M., Tachikawa, M., Odashima, H.
  • Journal Title: Jpn. J. Appl. Phys. 46
  • NAID: 40015671028
  • Description: 「研究成果報告書概要(欧文)」より
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• [Journal Article] Acetyl phosphate-sensitive regulation of flagellar biogenesis and capsular biosynthesis depends on the Rcsphosphorelay (2006)
  • Author(s): Fredericks, C.E., Shibata, S., Aizawa, S.-I, Reimann, S.A., and Wolfe, A.J.
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• [Journal Article] Characterising the flagellar filament and the role of motility in bacterial prey-penetration by Bdellovibriobacteriovorus (2006)
  • Author(s): Lambert, C., Evans, K.J., Till, R., Hobley, L., Capeness, M., Rendulic, S., Shuster, S., C., Aizawa, S.-I., and Sockett, E.
  • Journal Title: Mol Micro. 60 Pages: 274-286
  • Description: 「研究成果報告書概要(和文)」より
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• [Journal Article] Magnetic characterization of Daphinia Resting eggs (2006)
  • Author(s): Sakata, M., Kawasaki, T., Shibue, T., Takada, A., Yoshimura, H., Namiki, H.
  • Journal Title: BBRC 351 Pages: 566-570
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Protein-assisted Nanoparticle synthesis (2006)
  • Author(s): Yoshimura, H.
  • Journal Title: Colloids and Surfaces A 282-283 Pages: 464-470
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hundreds of the flagellar basal bodies cover the cell surface of the endosymbiotic bacterium Buchneraaphidicolasp (2006)
  • Author(s): Maezawa, K., Shigenobu, S., Taniguchi, H., Kubo, T., Aizawa, S.-I., Morioka, M.
  • Journal Title: APS. J. Bacteriol. 188 Pages: 6539-6543
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Acetyl phosphate-sensitive regulation of flagellar biogenesis and capsular biosynthesis depends on the Rcsphosphorelay (2006)
  • Author(s): Fredericks, C. E., Shibata, S., Aizawa, S.-I, Reimann, S. A., Wolfe, A. J.
  • Journal Title: Mol Micro. 61 Pages: 734-747
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterising the flagellar filament and the role of motility in bacterial prey-penetration by Bdellovibriobacteriovorus (2006)
  • Author(s): Lambert, C., Evans, K. J., Till, R., Hobley, L., Capeness, M., Rendulic, S., Shuster, S., C., Aizawa. S.-I., Sockett, E.
  • Journal Title: Mol Micro. 60 Pages: 274-286
  • Description: 「研究成果報告書概要(欧文)」より
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  • Author(s): G.H.Jeong, A.Yamazaki, S.Suzuki, H.Yoshimura, Y.Kobayashi, and Y.Homma
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  • Author(s): Shibata, S., Alam, M., and Aizawal, S.-I
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  • Description: 「研究成果報告書概要(和文)」より
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• [Journal Article] N-terminal signal region of FliK is dispensable for length control of the flagellar hook (2005)
  • Author(s): Hirano, T., Shibata, S., Ohnishi, K., Tani, T., &Aizawa, S.-I
  • Journal Title: Mol.Microbiol 56 Pages: 346-360
  • Description: 「研究成果報告書概要(和文)」より
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  • Author(s): O'Shea, T.M., DeLoney-Marino, C.R., Shibata, S., Aizawa, S.-I Wolfe, A.J., and Visick, K.L.
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  • Description: 「研究成果報告書概要(和文)」より
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  • Author(s): Aizawa, S.-I.
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• [Journal Article] Protease susceptibility of the Caulobactercrescentusflagellar Hook-Basal-Body;a possible mechanism of flagellar ejection during cell differentiation (2005)
  • Author(s): Kanbe, M., Shibata, S., Jenal, U., and Aizawa, S.-I.
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  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Self-Organized Inorganic Nanoparticle Arrays on Protein Lattices (2005)
  • Author(s): Okuda, M., Kobayashi, Y., Suzuki, K., Sonoda, K., Kondoh, T., Wagawa, A., Kondo, A., Yoshimura, H.
  • Journal Title: Nano Letters 5 Pages: 991-993
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Micro-CT of Pseudocneorhinusbifasciatus by projection X-ray microscopy (2005)
  • Author(s): Tanisako, A., Hori, A., Okumura, A., Miyata, C., Kuzuryu, C., Obi, T., Yoshimura, H.
  • Journal Title: J. Electron Microsc. 54 Pages: 379-383
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cobalt-Filled Apoferritin for Suspended Single-Walled Carbon Nanotube Growth with Narrow Diameter Distribution (2005)
  • Author(s): Jeong G. H., Yamazaki, A., Suzuki, S., Yoshimura, H., Kobayashi, Y., Homma, Y.
  • Journal Title: J. Am. Chem. Soc. 127 Pages: 8238-8239
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of nanoparticle density on narrow diameter distribution of carbon nanotubes and particle evolution during chemical vapor deposition growth (2005)
  • Author(s): Jeong, G. H., Suzuki, S., Kobayashi, Y., Yamazaki, A., Yoshimura, H., Homma, Y.
  • Journal Title: J. Appl. Phys. 98 Pages: 124311-124317
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dark resonance of Cs atoms velocity-selected in a thin cell (2005)
  • Author(s): Fukuda, K., Toriyama, A., Izmailov, A., Tachikawa, M.
  • Journal Title: Appl. Phys. B 80 Pages: 503-509
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Flagella of the deep-sea bacteria Idiomarinaloihiensis belong to a family different from Salmonella flagella (2005)
  • Author(s): Shibata, S., Alam, M., Aizawal, S,-I.
  • Journal Title: J. Mol. Biol. 352 Pages: 510-516
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] N-terminal signal region of FliK is dispensable for length control of the flagellar hook (2005)
  • Author(s): Hirano, T., Shibata, S., Ohnishi, K., Tani, T., Aizawa, S.-I.
  • Journal Title: Mol. Microbiol. 56 Pages: 346-360
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bacterial Gliding Motility : Visualizing Invisible Machinery (2005)
  • Author(s): Aizawa, S.-I.
  • Journal Title: ASM News feature article. 71 Pages: 71-76
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Protease susceptibility of the Caulobactercrescentusflagellar Hook-Basal-Body ; a possible mechanism of flagellar ejection during cell differentiation (2005)
  • Author(s): Kanbe, M., Shibata, S., Jenal, U., Aizawa, S.-I.
  • Journal Title: Microbiodogy 151 Pages: 433-438
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Magnesium promotes flagellation of Vibriofischeri (2005)
  • Author(s): O'shea, T. M., De Loney-Marino, C. R., Shibata, S., Aizawa, S.-I., Wolfe, A. J., Visick, K. L.
  • Journal Title: J. Bacteriol. 187 Pages: 2058-2065
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] バクテリアベん毛剛性率の直接的な測定 (2007)
  • Author(s): 原田 知明、保科 洋介、吉村 英恭
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2007-12-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ミジンコ(Daphnia magna)耐久卵内の超常磁性物質の粒子径 (2007)
  • Author(s): 坂田 真信、川崎 たまみ、渋江 俊道、吉村 英恭、並木 秀男
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2007-12-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] バクテリアベん毛剛性率の直接的な測定 (2007)
  • Author(s): 原田 知明、保科 洋介、吉村 英恭
  • Organizer: 日本生物物理学会
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2007-12-22
• [Presentation] タンパク質フェリチンを用いた硫化亜鉛ナノ粒子の作製 (2007)
  • Author(s): 小西 徹、大塚 泰之、吉村 英恭
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2007-12-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Microstructural Observation of Calcium-deficient Single Crystal Apatite Fibers and Phase Chanes during Heating (2007)
  • Author(s): Taki, A., Yoshimura, H., and Aizawa, M.
  • Organizer: Bioceramics 20
  • Place of Presentation: 千里ライフサイエンスセンター
  • Year and Date: 2007-11-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] In Vitro Synthesis of Calcium Nanoparticles Using the Protein Cage of Anoferritin (2007)
  • Author(s): Fukano, H., Aizawa, M., Yoshimura, H.
  • Organizer: Bioceramics 20
  • Place of Presentation: Nantes,France.
  • Year and Date: 2007-10-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 透過型電子顕微鏡によるケイ素含有アパタイトファイバーの微細構造観察 (2007)
  • Author(s): 瀧 絢子、深野 弘子、吉村 英恭、相澤 守
  • Organizer: バイオマテリアル学会
  • Place of Presentation: Nantes,France.
  • Year and Date: 2007-10-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Microstructural Observation of Calcium-deficient Single Crystal Apatite Fibers and Phase Changes during Heating (2007)
  • Author(s): Taki, A., Yoshimura, H., Aizawa, M.
  • Organizer: Bioceramics 20
  • Place of Presentation: Nantes, France
  • Year and Date: 2007-10-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] In Vitro Synthesis of Calcium Nanoparticles Using the Protein Cage of Apoferritin (2007)
  • Author(s): Fukano, H., Aizawa, M., Yoshimura, H.
  • Organizer: Bioceramics 20
  • Place of Presentation: Nantes, France
  • Year and Date: 2007-10-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Au-ferritinを用いた単層カーボンナノチューブ合成とSEM観察 (2007)
  • Author(s): 高木 大輔, 山嵜 明, 大塚 泰之, 吉村 英恭, 小林 慶裕, 本間 芳和
  • Organizer: 第68回秋季応用物理学会
  • Place of Presentation: 北海道工業大
  • Year and Date: 2007-09-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] フェリチン内包鉄ナノ粒子の構造評価 (2007)
  • Author(s): 山嵜 明、鈴木 友子、吉村 英恭、表和 彦、伊藤 義泰、川村 朋晃、小林 慶裕
  • Organizer: 第68回秋季応用物理学会
  • Place of Presentation: 北海道工業大
  • Year and Date: 2007-09-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] X線小角散乱法による鉄内包フェリチンの構造評価 (2007)
  • Author(s): 伊藤 義泰、表和 彦、山嵜 明、鈴木 友子、吉村 英恭
  • Organizer: 第68回秋季応用物理学会
  • Place of Presentation: 北海道工業大
  • Year and Date: 2007-09-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 疥癬虫の放射光によるMicro-CT (2007)
  • Author(s): 吉村 英恭, 大東 琢治, 上椙 真之, 上杉 健太朗, 篠原 邦夫, 東川 俊昭, 中村 立一, 森 喜紀
  • Organizer: 第68回秋季応用物理学会
  • Place of Presentation: 北海道工業大
  • Year and Date: 2007-09-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Gold-filled apoferritin catalyst for aligned single-walled carbon nanotube growth on sapphire substrates (2007)
  • Author(s): Yamazaki, A., Takagi, D., Otsuka, Y., Suzuki, S., Homma, Y., Yoshimura, H., and Kobayasbi, Y.
  • Organizer: 33rd Fullerenes&NanotubesSymp
  • Place of Presentation: 九州大学
  • Year and Date: 2007-07-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Growth behavior of aligned single-walled carbonnanotubes on sapphire surface (2007)
  • Author(s): Yamazaki, A., Takagi, D., Suzuki, S., Jeong, G.H., Yoshimura, H., Homma, Y., Kobayashi, Y.
  • Organizer: 13rd international conference on Surface Science
  • Place of Presentation: Stockholm,Sweden
  • Year and Date: 2007-07-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Growth behavior of aligned single-walled carbonnanotubes on sapphire surface (2007)
  • Author(s): Yamazaki, A., Takagi, D. Suzuki, S., Jeong, G. H., Yoshimura, H., Homma, Y. Kobayashi, Y.
  • Organizer: 13rd international conference on Surtace Science
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2007-07-05
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] フレネル回折ボケ像の修正とその軟X線顕微鏡への応用 (2007)
  • Author(s): 鈴木 剛志、本田 捷夫、椎名 達雄、伊藤 敦、金城 康人、吉村 英恭
  • Organizer: 光計測シンポジウム
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-06-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] サファイア基盤上単層カーボンナノチューブの配向成長とその機構 (2007)
  • Author(s): 山嵜 明、高木 大輔、鈴木 哲、Goo・Hwan Jeong、吉村 英恭、本間 芳和、小林 慶裕
  • Organizer: 第54回応用物理学会
  • Place of Presentation: 青山学院大学
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] X線顕微鏡を用いた元素解析 (2007)
  • Author(s): 鶴田 進、吉村 英恭
  • Organizer: 第54回応用物理学会
  • Place of Presentation: 青山学院大学
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カルシウム欠損型アパタイトファイバーの微細構造観察 (2007)
  • Author(s): 瀧 絢子、吉村 英恭、相澤 守
  • Organizer: 日本セラミックス協会
  • Place of Presentation: 武蔵工業大学
  • Year and Date: 2007-03-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Size control of catalytic nanoparticles by thermal treatment toward diameter control of single-walled carbon nanotubes (2007)
  • Author(s): Yamazaki, A., Jeong, G.H., Suzuki, S., Yoshimura, H., Homma, Y., Kobayashi, Y.
  • Organizer: 32nd Fullerenes&NanotubesSymp
  • Place of Presentation: 名城大学
  • Year and Date: 2007-02-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Aligned growth of single-walled carbon nanotubes due to interaction with sapphire substrates (2007)
  • Author(s): Yamazaki, A., Takagi, D., Suzuki, S., Jeong, G.H., Yoshimura, H., Homma, Y., Kobayashi, Y.
  • Organizer: 32nd Fullerenes&NanotubesSymp
  • Place of Presentation: 名城大学
  • Year and Date: 2007-02-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 高分解能透過型電子顕微鏡によるカルシウム欠損型単結晶アパタイトファイバーの微細構造観察 (2006)
  • Author(s): 瀧 絢子、吉村 英恭、相澤 守
  • Organizer: 第28回日本バイオマテリアル学会
  • Place of Presentation: アルカディア市ケ谷
  • Year and Date: 2006-11-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Magnetic Characterization of Daphinia resting eggs (2006)
  • Author(s): Sakata, M., Kawasaki, T., Shibue, T., Tsuruta, S., Yoshimura, H., Namiki, H.
  • Organizer: 第5回東アジア生物物理学シンポジウム合同会議
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2006-11-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Imaging of living tissue culture cell by AFM and adeforming effect under high pressureenvironment (2006)
  • Author(s): Yamamoto, Y., Yoshimura, H., Miwa, T.
  • Organizer: 第5回東アジア生物物理学シンポジウム合同会議
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2006-11-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Projection X-ray microscopy of Daphinia with eggs (2006)
  • Author(s): Tsuruta, S., Kawasaki, T., Sakata, M., Namiki, H., Yoshimura, H.
  • Organizer: 第5回東アジア生物物理学シンポジウム合同会議
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2006-11-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] In vitro synthesis of calcium nanoparticles using the globular protein cavity (2006)
  • Author(s): Fukano, H., Yoshimura, H., Aizawa, M.
  • Organizer: 第5回東アジア生物物理学シンポジウム合同会議
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2006-11-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Fabrication of gold nanoparticles using the cage protein ferritin (2006)
  • Author(s): Otsuka, Y., Konishi, T., Aoki, H., Yamazaki, A., Yoshizawa, K., Yamashita, I., Yoshimura, H.
  • Organizer: 第5回東アジア生物物理学シンポジウム合同会議
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2006-11-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Fabrication of molybdenum nanoparticles using the globular protein cavity (2006)
  • Author(s): Kaneko, T., Otsuka, Y., Konishi, T., Yoshimura, H.
  • Organizer: 第5回東アジア生物物理学シンポジウム合同会議
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2006-11-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Site-selective deposition of ferritin on the patterned silicon substrates (2006)
  • Author(s): Yamazaki, A., Fukano, H., Yoshimura, H.
  • Organizer: 第5回東アジア生物物理学シンポジウム合同会議
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2006-11-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] CT Reconstruction of Human Cell with Soft X-ray Projection CT Microscope (2006)
  • Author(s): Suzuki, T., Tsuge, Y., Shiina, T., Honda, T., Yoshimura, H., Kinjo Y., and Ito, A
  • Organizer: The 16th International Microscopy Congess
  • Place of Presentation: Sapporo
  • Year and Date: 2006-09-03
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Soft X-ray Projection CT Microscope (2006)
  • Author(s): Shiina, T., Suzuki, T., Tsuge, Y., Honda, T., Yoshimura, H., Kinjo, Y., and Ito, A
  • Organizer: The 16th International Microscopy Congess
  • Place of Presentation: Sapporo
  • Year and Date: 2006-09-03
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] サイズ制御触媒を用いたサファイア基板上での単層カーボンナノチューブ (2006)
  • Author(s): 山嵜 明、高木 大輔、鈴木 哲、Goo-Hwan Jeong、小林 慶裕、吉村 英恭、本間 芳和
  • Organizer: 第67回応用物理学会学術講演会
  • Place of Presentation: 立命館大学
  • Year and Date: 2006-08-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Synthesis and regular array formation of nano-particlesassisted by protein (2006)
  • Author(s): Okuda, M., Kobayashi, Y., Kondoh, T., Konishi, T., Yoshimura, H.
  • Organizer: SPIE Optics & Photonics
  • Place of Presentation: San Diego USA
  • Year and Date: 2006-08-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Micro-CT of small insects by projection X-ray microscopy (2006)
  • Author(s): Tanisako, A., Tsuruta, S., Hori, A., Okumura, A., Miyata, C., Kuzuryu, C., Obi, T., Yoshimura, H.
  • Organizer: SPIE Optics & Photonics
  • Place of Presentation: San Diego USA
  • Year and Date: 2006-08-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Aligned SWNT growth using size-controlled catalysts on sapphire (2006)
  • Author(s): 山嵜 明、高木 大輔、鈴木 哲、Goo-Hwan Jeong、小林 慶裕、吉村 英恭、本間 芳和
  • Organizer: 31St., Fullerenes &NanotubesSymp
  • Place of Presentation: 三重県総合文化センター
  • Year and Date: 2006-07-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Single-walled carbon nanotubes from catalyst incorporating proteins (2006)
  • Author(s): Jeong, G-H., Yamazaki, A., Suzuki, S., Kobayashi, Y., Yoshimura, H., Homma, Y.
  • Organizer: NANOTUBE 2006
  • Place of Presentation: ホテルメトロポリタン長野
  • Year and Date: 2006-06-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] バクテリアベん毛モータの潤滑駆動機構を通して量子は見えるか (2006)
  • Author(s): 中野 隆、桃園 聡、相沢 慎一、吉村 英恭
  • Organizer: トライボロジー会議
  • Place of Presentation: 東京
  • Year and Date: 2006-05-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Two-dimellsional crystallization of proteins for Nano-technology (2006)
  • Author(s): Yoshimura, H.
  • Organizer: Nano-Bio Interface 2006
  • Place of Presentation: Calcutta,India.
  • Year and Date: 2006-03-02
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Synthesis of uniform Nanoparticles using nano-cage of Proteins (2006)
  • Author(s): Yoshimura, H.
  • Organizer: Nano-Bio Interface 2006
  • Place of Presentation: Calcutta,India.
  • Year and Date: 2006-03-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Synthesis of uniform Nanoparticles using nano-cage of Proteins (2006)
  • Author(s): Yoshimura, H.
  • Organizer: Nano-Bio Interface 2006
  • Place of Presentation: Calcutta, India.(invited)
  • Year and Date: 2006-03-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Two-dimensional crystallization of proteins for Nano-technology (2006)
  • Author(s): Yoshimura, H.
  • Organizer: Nano-Bio Interface 2006
  • Place of Presentation: Calcutta, India.(invited)
  • Year and Date: 2006-03-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] サルモネラ菌べん毛フックの柔らかさ (2005)
  • Author(s): 保科 洋介、吉村 英恭
  • Organizer: 第43回日本生物物理学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2005-11-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Magnetic tests and ferromagnetic resonance on Daphnia resting eggs (2005)
  • Author(s): 坂田 真信、川崎 たまみ、渋江 俊道、鶴田 進、吉村 英恭、並木 秀男
  • Organizer: 第43回日本生物物理学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2005-11-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] フェリチンを用いた格子欠陥のない鉄ナノ粒子の2次元配列の作製 (2005)
  • Author(s): 近藤 哲郎、小林 悠作、奥田 充宏、鈴木 友子、深野 弘子、吉村 英恭
  • Organizer: 第43回日本生物物理学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2005-11-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] タンパク質素材によるアルミニウムナノ粒子の作製 (2005)
  • Author(s): 小西 徹、近藤 哲郎、大塚 泰之、金子 嵩之、深野 弘子、吉村 英恭
  • Organizer: 第43回日本生物物理学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2005-11-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 投影型X線顕微鏡を用いた元素解析 (2005)
  • Author(s): 鶴田 進、坂田 真信、川崎 たまみ、並木 秀男、吉村 英恭
  • Organizer: 第43回日本生物物理学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2005-11-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] サルモネラ菌べん毛フックのやわらかさ (2005)
  • Author(s): 保科 洋介、吉村 英恭
  • Organizer: 第66回応用物理学会学術講演会
  • Place of Presentation: 徳島大学
  • Year and Date: 2005-09-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] フェリチンに内包された金属ナノ粒子の内径分布 (2005)
  • Author(s): 吉村 英恭、鈴木 友子、深野 弘子、近藤 哲郎、小林 悠作、奥田 充宏
  • Organizer: 第66回応用物理学会学術講演会
  • Place of Presentation: 徳島大学
  • Year and Date: 2005-09-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] タンパク質フェリチンを用いた効率的な鉄ナノ粒子の作製 (2005)
  • Author(s): 近藤 哲郎、奥田 充宏、小林 悠作、鈴木 友子、深野 弘子、吉村 英恭
  • Organizer: 第66回応用物理学会学術講演会
  • Place of Presentation: 徳島大学
  • Year and Date: 2005-09-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Apprication of projection x-ray microscopy to biological phenomena (2005)
  • Author(s): Yoshimura, H.
  • Organizer: The 10th keihanna Conference on Molecular Biophyscis
  • Place of Presentation: Keihanna Plaza,Kyoto
  • Year and Date: 2005-07-31
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] CT reconstruction by diffraction correction in soft x-ray projection microscopy (2005)
  • Author(s): Shiina, T., Hotta, K., Tsuge, Y., Honda, T., Yoshimura, H., Kinjyo, Y., Ito, A
  • Organizer: The 8th International Conference on X-ray microsvcopy
  • Place of Presentation: Himeji
  • Year and Date: 2005-07-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Micro-CT of Pseudocneorhinusbifasciatus by x-ray microscopy (2005)
  • Author(s): Tanisako, A., Hori, A., Okumura, A., Miyata, C., Kuzuryu, C., Yoshimura, H.
  • Organizer: The 8th International Conference on X-ray microsvcopy
  • Place of Presentation: Himeji
  • Year and Date: 2005-07-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Micro-CT of Pseudocneorhinusbifasciatus by x-ray microscopy (2005)
  • Author(s): Tanisako, A., Hori, A., Okumura, A., Miyata, C., Kuzuryu, C., Yoshimura, H.
  • Organizer: The 8th International Conference on X-ray microsvcopy(XRM2005)
  • Place of Presentation: Himeji(Japan)
  • Year and Date: 2005-07-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] CT reconstruction by diffraction correction in soft x-ray projection microscopy (2005)
  • Author(s): Shiina, T., Hotta, K., Tsuge, Y., Honda, T., Yoshimura, H., Kinjyo, Y., Ito, A.
  • Organizer: The 8th International Conference on X-ray microsvcopy
  • Place of Presentation: Himeji(Japan)
  • Year and Date: 2005-07-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 走査電子顕微鏡を改造した投影型X線顕微鏡を用いた昆虫のマイクロCT (2005)
  • Author(s): 谷迫 章、吉村 英恭
  • Organizer: 日本顕微鏡学会第61回学術講演会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2005-06-02
  • Description: 「研究成果報告書概要(和文)」より
• [Book] いのちの物理-生物物理学はまじめの一歩- (2006)
  • Author(s): 吉村 英恭
  • Total Pages: 160
  • Publisher: 桐書房
  • Description: 「研究成果報告書概要(和文)」より