Mechanical properties of bacterial hook filament
Project/Area Number |
17340126
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biophysics/Chemical physics
|
Research Institution | Meiji University |
Principal Investigator |
YOSHIMURA Hideyuki Meiji University, Department of Physics, Professor (70281441)
|
Co-Investigator(Kenkyū-buntansha) |
NAKANO Takashi Tokyo Institute of Technology, Department of Mechanical and Aerospace Engineering, Assistant (00183517)
MOMOZONO Satoshi Tokyo Institute of Technology, Department of Mechanical and Aerospace Engineering, Assistant (70262300)
AIZAWA Shin-ichi Prefectural University of Hiroshima, Department of Life Science, Professor (50222451)
TACHIKAWA Mki Meiji University, Department of Physics, Professor (60201612)
|
Project Period (FY) |
2005 – 2007
|
Project Status |
Completed (Fiscal Year 2007)
|
Budget Amount *help |
¥16,490,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥690,000)
Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2005: ¥11,400,000 (Direct Cost: ¥11,400,000)
|
Keywords | flagella / polyhook / Young's modulus / rigidity / fluorescence / electronmicroscopy / rapid freezing / freeze dry / レーザトラップ |
Research Abstract |
Flagellated bacteria like Sal. Typhimurium swims by rotating helical flagella filaments with bundling and synchronizing them each other. Because axs of bacterial motors directed in different way, flexible joint part is required between motor and flagella filament. It is known that bacterial hook work as a universal joint of this part. However its mechanical feature like rigidity is not known exactly. Here we measured modulus of rigidity by using polyhook filament, length about 1μm, from mutant Sal. Typhimurium(SWJ108). Purified polyhook filaments were frozen rapidly in liquid ethane and freeze dried. Distribution of helix pitch and diameter were evaluated from electron micrographs of shadowed images. We got value of 95±23nm for helix pitch and 31±9.9nm for diameter of polyhook filament. We also measured fluctuation of helical pitch and diameter of flagella filament from negative stained samples. By comparing fluctuation of polyhook and flagella filaments, we have calculated the ratio of rigidity as G_<flagella>/G<hook>=120. According to the literature value of flagella filament rigidity, 3.8×10^9Pa, we obtained rigidity of polyhook as 3.3×10^7Pa. We also tried to measure the rigidity of flagella in solution directly using thin glass capillary. Fluorescent labeled flagella filament was fixed by anti-flagellin adsorbed on a glass capillary of diameter less than 1μm. Tension in pN range was measured from bending deformation of glass capillary under fluorescent microscope. We are now accumulating the results in this process. We are going to measure rigidity of super-poly hook which has length of few micro-meter by the same method.
|
Report
(4 results)
Research Products
(115 results)
-
-
-
-
-
-
-
-
-
[Journal Article] Systematic deletion analysis of the fla genes in the flagella operon identify several genes essential for proper assembly and function of flagella in the archaeon, Methanococcusmaripaludis2007
Author(s)
Chaban, B., Ng, S.Y.M., Kanbe, M., Saltzman, I., Ninuno, G., Aizawa, S.-I., and Jarrel, K.F.
-
Journal Title
Mol.Micro. 66
Pages: 596-609
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
[Journal Article] The mechanism of outer membrane penetration by the eubacterial flagellum and implications for spirochete evolution2007
Author(s)
Chevance, F.F., Takahashi, N., Karlinsey, J.E., Gnerer, J., Hirano, T., Samudrala, R., Aizawa, S.-I, and Hughes, K.T.,
-
Journal Title
Genes Dev. 21
Pages: 2326-2335
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
-
[Journal Article] BifunctionalNanotube Scaffolds for Diverse Ligands Are Purified Simply from Escherichia coli Strains Coexpressing Two Functionalized Flagellar Genes2007
Author(s)
Woods, R.D., Takahashi, N., Aslam, A., Pleass, R.J., Aizawa, S-I., and Sockett, R.E.
-
Journal Title
NanoLett. 7
Pages: 1809-1816
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
-
[Journal Article] T Characterization of two sets of sub-polar flagella in Bradyrhizobiumjaponicum2007
Author(s)
Shibata, S., Takahashi, N., Chevance, F.F.V., Karlinsey, J.E., Hughes, K.T., and Aizawa, S.-I.
-
Journal Title
Mol Micro. 64
Pages: 1404-1415
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
-
-
[Journal Article] Size Control of catalytic nanoparticles by thermal treament and its application to diameter control of single-walled carbon nanotubes2007
Author(s)
Jeong, G. H., Suzuki, S., Kobayashi, Y., Yamazaki, A., Yoshimura, H., Homma, Y.
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Journal Article] Gold-filled apo-ferritin for investigation of single-walled carbon nanotube growth on substrate2007
Author(s)
Takagi, D., Yamazaki, A., Otsuka, Y., Yoshimura, H., Kobayashi, Y., Homma, Y.
-
Journal Title
Chem. Phys. Lett. 445
Pages: 213-216
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Systematic deletion analysis of the fla genes in the flagella operon identify several genes essential for proper assembly and function of flagella in the archaeon, Methanococcusmaripaludis2007
Author(s)
Chaban, B., Ng, S. Y. M., Kanbe, M., Saltzman, I., Nimmo, G., Aizawa, S.-I., Jarred, K. F.
-
Journal Title
Mol. Micro. 66
Pages: 596-609
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] The mechanism of outer membrane penetration by the eubacterial flagellum and implications for spirochete evolution2007
Author(s)
Chevance, F. F., Takahashi, N., Karlinsey, J. E., Gnerer, J., Hirano, T., Samudrala, R., Aizawa, S,-I, Hughes, K. T.
-
Journal Title
Genes. Dev. 21
Pages: 2326-2335
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Journal Article] Bifunctional Nanotube Scaffolds for Diverse Ligands Are Purified Simply from Escherichia coli Strains Coexpressing Two Functionalized Flagellar Genes2007
Author(s)
Woods, R. D., Takahashi, N., Aslam, A., Pleass, R. J., Aizawa, S-I., Sockett, R. E.
-
Journal Title
NanoLett. 7
Pages: 1809-1816
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Journal Article] FliK regulates flagellar hook length as an internal ruler2007
Author(s)
Shibata, S., Takahashi, N., Chevance, F. F. V., Karlinsey, J. E., Hughes, K. T., Aizawa, S.-I.
-
Journal Title
Mol Micro. 64
Pages: 1404-1415
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
-
-
[Journal Article] Hundreds of the flagellar basal bodies cover the cell surface of the endo symbiotic bacterium Buchneraaphidicolasp2006
Author(s)
Maezawa, K., Shigenobu, S., Taniguchi, H., Kubo, T., Aizawa, S.-I., Morioka, M.
-
Journal Title
APS.J.Bacteriol. 188
Pages: 6539-6543
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
-
-
[Journal Article] Characterising the flagellar filament and the role of motility in bacterial prey-penetration by Bdellovibriobacteriovorus2006
Author(s)
Lambert, C., Evans, K.J., Till, R., Hobley, L., Capeness, M., Rendulic, S., Shuster, S., C., Aizawa, S.-I., and Sockett, E.
-
Journal Title
Mol Micro. 60
Pages: 274-286
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
[Journal Article] Magnetic characterization of Daphinia Resting eggs2006
Author(s)
Sakata, M., Kawasaki, T., Shibue, T., Takada, A., Yoshimura, H., Namiki, H.
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Journal Article] Hundreds of the flagellar basal bodies cover the cell surface of the endosymbiotic bacterium Buchneraaphidicolasp2006
Author(s)
Maezawa, K., Shigenobu, S., Taniguchi, H., Kubo, T., Aizawa, S.-I., Morioka, M.
-
Journal Title
APS. J. Bacteriol. 188
Pages: 6539-6543
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Journal Article] Characterising the flagellar filament and the role of motility in bacterial prey-penetration by Bdellovibriobacteriovorus2006
Author(s)
Lambert, C., Evans, K. J., Till, R., Hobley, L., Capeness, M., Rendulic, S., Shuster, S., C., Aizawa. S.-I., Sockett, E.
-
Journal Title
Mol Micro. 60
Pages: 274-286
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
-
-
-
-
[Journal Article] Magnesium promotes flagellation of Vibriofischeri2005
Author(s)
O'Shea, T.M., DeLoney-Marino, C.R., Shibata, S., Aizawa, S.-I Wolfe, A.J., and Visick, K.L.
-
Journal Title
J.Bacteriol. 187
Pages: 2058-2065
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Self-Organized Inorganic Nanoparticle Arrays on Protein Lattices2005
Author(s)
Okuda, M., Kobayashi, Y., Suzuki, K., Sonoda, K., Kondoh, T., Wagawa, A., Kondo, A., Yoshimura, H.
-
Journal Title
Nano Letters 5
Pages: 991-993
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Micro-CT of Pseudocneorhinusbifasciatus by projection X-ray microscopy2005
Author(s)
Tanisako, A., Hori, A., Okumura, A., Miyata, C., Kuzuryu, C., Obi, T., Yoshimura, H.
-
Journal Title
J. Electron Microsc. 54
Pages: 379-383
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Cobalt-Filled Apoferritin for Suspended Single-Walled Carbon Nanotube Growth with Narrow Diameter Distribution2005
Author(s)
Jeong G. H., Yamazaki, A., Suzuki, S., Yoshimura, H., Kobayashi, Y., Homma, Y.
-
Journal Title
J. Am. Chem. Soc. 127
Pages: 8238-8239
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Effect of nanoparticle density on narrow diameter distribution of carbon nanotubes and particle evolution during chemical vapor deposition growth2005
Author(s)
Jeong, G. H., Suzuki, S., Kobayashi, Y., Yamazaki, A., Yoshimura, H., Homma, Y.
-
Journal Title
J. Appl. Phys. 98
Pages: 124311-124317
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
-
-
-
[Journal Article] Magnesium promotes flagellation of Vibriofischeri2005
Author(s)
O'shea, T. M., De Loney-Marino, C. R., Shibata, S., Aizawa, S.-I., Wolfe, A. J., Visick, K. L.
-
Journal Title
J. Bacteriol. 187
Pages: 2058-2065
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 疥癬虫の放射光によるMicro-CT2007
Author(s)
吉村 英恭, 大東 琢治, 上椙 真之, 上杉 健太朗, 篠原 邦夫, 東川 俊昭, 中村 立一, 森 喜紀
Organizer
第68回秋季応用物理学会
Place of Presentation
北海道工業大
Year and Date
2007-09-06
Description
「研究成果報告書概要(和文)」より
Related Report
-
[Presentation] Gold-filled apoferritin catalyst for aligned single-walled carbon nanotube growth on sapphire substrates2007
Author(s)
Yamazaki, A., Takagi, D., Otsuka, Y., Suzuki, S., Homma, Y., Yoshimura, H., and Kobayasbi, Y.
Organizer
33rd Fullerenes&NanotubesSymp
Place of Presentation
九州大学
Year and Date
2007-07-12
Description
「研究成果報告書概要(和文)」より
Related Report
-
[Presentation] Growth behavior of aligned single-walled carbonnanotubes on sapphire surface2007
Author(s)
Yamazaki, A., Takagi, D., Suzuki, S., Jeong, G.H., Yoshimura, H., Homma, Y., Kobayashi, Y.
Organizer
13rd international conference on Surface Science
Place of Presentation
Stockholm,Sweden
Year and Date
2007-07-05
Description
「研究成果報告書概要(和文)」より
Related Report
-
[Presentation] Growth behavior of aligned single-walled carbonnanotubes on sapphire surface2007
Author(s)
Yamazaki, A., Takagi, D. Suzuki, S., Jeong, G. H., Yoshimura, H., Homma, Y. Kobayashi, Y.
Organizer
13rd international conference on Surtace Science
Place of Presentation
Stockholm, Sweden
Year and Date
2007-07-05
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
[Presentation] X線顕微鏡を用いた元素解析2007
Author(s)
鶴田 進、吉村 英恭
Organizer
第54回応用物理学会
Place of Presentation
青山学院大学
Year and Date
2007-03-28
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
[Presentation] Size control of catalytic nanoparticles by thermal treatment toward diameter control of single-walled carbon nanotubes2007
Author(s)
Yamazaki, A., Jeong, G.H., Suzuki, S., Yoshimura, H., Homma, Y., Kobayashi, Y.
Organizer
32nd Fullerenes&NanotubesSymp
Place of Presentation
名城大学
Year and Date
2007-02-15
Description
「研究成果報告書概要(和文)」より
Related Report
-
[Presentation] Aligned growth of single-walled carbon nanotubes due to interaction with sapphire substrates2007
Author(s)
Yamazaki, A., Takagi, D., Suzuki, S., Jeong, G.H., Yoshimura, H., Homma, Y., Kobayashi, Y.
Organizer
32nd Fullerenes&NanotubesSymp
Place of Presentation
名城大学
Year and Date
2007-02-15
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
[Presentation] Magnetic Characterization of Daphinia resting eggs2006
Author(s)
Sakata, M., Kawasaki, T., Shibue, T., Tsuruta, S., Yoshimura, H., Namiki, H.
Organizer
第5回東アジア生物物理学シンポジウム合同会議
Place of Presentation
沖縄コンベンションセンター
Year and Date
2006-11-14
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
-
-
[Presentation] Fabrication of gold nanoparticles using the cage protein ferritin2006
Author(s)
Otsuka, Y., Konishi, T., Aoki, H., Yamazaki, A., Yoshizawa, K., Yamashita, I., Yoshimura, H.
Organizer
第5回東アジア生物物理学シンポジウム合同会議
Place of Presentation
沖縄コンベンションセンター
Year and Date
2006-11-14
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
-
[Presentation] CT Reconstruction of Human Cell with Soft X-ray Projection CT Microscope2006
Author(s)
Suzuki, T., Tsuge, Y., Shiina, T., Honda, T., Yoshimura, H., Kinjo Y., and Ito, A
Organizer
The 16th International Microscopy Congess
Place of Presentation
Sapporo
Year and Date
2006-09-03
Description
「研究成果報告書概要(和文)」より
Related Report
-
[Presentation] Soft X-ray Projection CT Microscope2006
Author(s)
Shiina, T., Suzuki, T., Tsuge, Y., Honda, T., Yoshimura, H., Kinjo, Y., and Ito, A
Organizer
The 16th International Microscopy Congess
Place of Presentation
Sapporo
Year and Date
2006-09-03
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
-
[Presentation] Micro-CT of small insects by projection X-ray microscopy2006
Author(s)
Tanisako, A., Tsuruta, S., Hori, A., Okumura, A., Miyata, C., Kuzuryu, C., Obi, T., Yoshimura, H.
Organizer
SPIE Optics & Photonics
Place of Presentation
San Diego USA
Year and Date
2006-08-14
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
[Presentation] Single-walled carbon nanotubes from catalyst incorporating proteins2006
Author(s)
Jeong, G-H., Yamazaki, A., Suzuki, S., Kobayashi, Y., Yoshimura, H., Homma, Y.
Organizer
NANOTUBE 2006
Place of Presentation
ホテルメトロポリタン長野
Year and Date
2006-06-18
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
-
-
-
-
[Presentation] サルモネラ菌べん毛フックの柔らかさ2005
Author(s)
保科 洋介、吉村 英恭
Organizer
第43回日本生物物理学会
Place of Presentation
札幌コンベンションセンター
Year and Date
2005-11-25
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
-
-
-
-
-
-
-
[Presentation] CT reconstruction by diffraction correction in soft x-ray projection microscopy2005
Author(s)
Shiina, T., Hotta, K., Tsuge, Y., Honda, T., Yoshimura, H., Kinjyo, Y., Ito, A
Organizer
The 8th International Conference on X-ray microsvcopy
Place of Presentation
Himeji
Year and Date
2005-07-27
Description
「研究成果報告書概要(和文)」より
Related Report
-
[Presentation] Micro-CT of Pseudocneorhinusbifasciatus by x-ray microscopy2005
Author(s)
Tanisako, A., Hori, A., Okumura, A., Miyata, C., Kuzuryu, C., Yoshimura, H.
Organizer
The 8th International Conference on X-ray microsvcopy
Place of Presentation
Himeji
Year and Date
2005-07-27
Description
「研究成果報告書概要(和文)」より
Related Report
-
[Presentation] Micro-CT of Pseudocneorhinusbifasciatus by x-ray microscopy2005
Author(s)
Tanisako, A., Hori, A., Okumura, A., Miyata, C., Kuzuryu, C., Yoshimura, H.
Organizer
The 8th International Conference on X-ray microsvcopy(XRM2005)
Place of Presentation
Himeji(Japan)
Year and Date
2005-07-27
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Presentation] CT reconstruction by diffraction correction in soft x-ray projection microscopy2005
Author(s)
Shiina, T., Hotta, K., Tsuge, Y., Honda, T., Yoshimura, H., Kinjyo, Y., Ito, A.
Organizer
The 8th International Conference on X-ray microsvcopy
Place of Presentation
Himeji(Japan)
Year and Date
2005-07-27
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-