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Study on Accumulation Mechanisms of As and Cd in Hyperaccumulator Plants by X-ray Analysis and its Application for Phytoremediation of Heavy Metals

Research Project

Project/Area Number 17350040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionTokyo University of Science

Principal Investigator

NAKAI Izumi  Tokyo University of Science, Department of Applied Chemistyr Faculty of Science, Professor, 理学部, 教授 (90155648)

Co-Investigator(Kenkyū-buntansha) HOKURA Akiko  Tokyo University of Science, Department of Applied Chemistyr Faculty of Science, Research assistant, 理学部, 助教 (20343569)
TERADA Yasuko  JASRI, Research & Utilization Division, Senior researcher, 利用研究促進部門・XAFS・分析チーム, 副主幹研究員 (90307695)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2006: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥11,600,000 (Direct Cost: ¥11,600,000)
Keywordssynchrotron radiation micro beam / XAFS / chemical speciation analysis / phytoremediation / arsenic / cadmium / high-energy X-ray / elemental mapping / ファイトレメディエーション
Research Abstract

We have developed an in vivo micro-X-ray fluorescence (μ-XRF) imaging technique and X-ray absorption near edge structure (XANES) analysis utilizing synchrotron radiation (SR) to determine the distribution of arsenic and cadmium in the tissues and cells of hyperaccumulator plants and to investigate their physiology and mechanism of heavy-metal accumulations. These plants are expected to be used for phytoremediation.
The arsenic distribution in the arsenic hyper-accumulating fern, Pteris vittata L., was investigated using a SR-XRF microprobe. The focused microbeam (3.5 x 5.5 μm2) produced by Kirkpatrick-Baez optics was applied to the fern to reveal the elemental distribution in plant tissue and cell levels. The results indicated that high levels of arsenic accumulate at the base of sporangium with lamina of pinnae. Also, the fern was subjected to XANES analysis without any sample treatment to elucidate directly the arsenic oxidation state in fern. It was found that arsenic exists as the As(III) form in pinnae, and as a mixture of As(III) and As(V) in rachis, while As(V) is present in cultivated soil. These findings indicate that the fern uptakes arsenic as As(V) from soil and that the As(V) is then partially reduced to As(III) within the plant, and finally accumulated as As(III) in a specific area of the pinna.
On the other hand, μ-XRF imaging using high-energy SR X-ray was carried out on trichomes prepared from the leaves of Arabidopsis halleri ssp. gemmifera, cadmium hyperaccumulating plant. The two-dimensional cellular distribution of cadmium in the trichomes was first observed by in vivo μ-XRF imaging. It was found that both cadmium and zinc accumulated at high level in the base of the bifurcation area of the trichomes, whereas strontium and calcium were mostly distributed throughout the entire upper part of the trichomes. These distributions of cadmium and zinc showed striking subcellular compartmentation.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (23 results)

All 2006 2005 Other

All Journal Article (23 results)

  • [Journal Article] 2-D X-ray Fluorescence Imaging of Cadmium Hyperaccumulating Plants by Using High-energy Synchrotron Radiation X-ray Microbeam2006

    • Author(s)
      Akiko Hokura, Ryoko Onuma, Nobuyuki Kitajima, Yasuko Terada, Hiroyuki Saito, Tomoko Abe, Shigeo Yoshida, Izumi Nakai
    • Journal Title

      CHEMISTRY LETTERS 35(11)

      Pages: 1246-1247

    • NAID

      10018404846

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 放射光蛍光X線分析を用いるヒ素高集積植物モエジマシダの根におけるヒ素の分布及び化学形態分析2006

    • Author(s)
      柏原輝彦, 保倉明子, 北島信行, 小沼亮子, 斉藤宏之, 阿部知子, 中井 泉
    • Journal Title

      分析化学 55(10)

      Pages: 743-748

    • NAID

      110004823097

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Arsenic Distribution and Speciation in the Arsenic Hyperaccumulator Fern by Micro-XRF Imaging and Micro-XANES Analysis2006

    • Author(s)
      Ryoko Onuma, Akiko Hokura, Nobuyuki Kitajima, Izumi Nakai, Yasuko Terada, Tomoko Abe, Hiroyuki Saito, Shigeo Yoshida
    • Journal Title

      Proc. 8th Int. Conf. X-ray Microscopy, IPAP Conference series 7

      Pages: 326-327

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cadmium Distribution in a Cadmium Hyperaccumulator Plant as Determined by Micro-XRF Imaging2006

    • Author(s)
      Akiko Hokura, Ryoko Onuma, Nobuyuki Kitajima, Izumi Nakai, Yasuko Terada, Tomoko Abe, Hiroyuki Saito, Shigeo Yoshida
    • Journal Title

      Proc. 8th Int. Conf. X-ray Microscopy, IPAP Conference series 7

      Pages: 323-325

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Distribution and XANES of Arsenic in Root of Hyperaccumulator Fern (Pteris vittata L.) Measured by μ-SR-XRF Analysis2006

    • Author(s)
      Nobuyuki Kitajima, Ryoko Onuma, Akiko Hokura, Izumi Nakai, Yasuko Terada
    • Journal Title

      Proc. 8th Int. Conf. X-ray Microscopy, IPAP Conference series 7

      Pages: 321-322

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 放射光X線マイクロビームを用いた重金属蓄積植物の蛍光X線イメージング2006

    • Author(s)
      小沼亮子, 中井 泉
    • Journal Title

      化学と生物 44(12)

      Pages: 860-864

    • NAID

      10018410331

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 2-D X-ray Fluorescence Imaging of Cadmium Hyperaccumulating Plants by Using High-energy Synchrotron Radiation X-ray Microbeam2006

    • Author(s)
      Akiko Hokura, et al.
    • Journal Title

      CHEMISTRY LETTERS 35(11)

      Pages: 1246-1247

    • NAID

      10018404846

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Distribution and Oxidation State of Arsenic in Root of Arsenic-Hyperaccumulator Fern,2006

    • Author(s)
      Teruhiko Kashiwabara, et al.
    • Journal Title

      BUNSEKI KAGAKU 55(10)

      Pages: 743-748

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] by Using Synchrotron Radiation X-ray Fluorescence Analysis2006

    • Author(s)
      Pteris vittata L.
    • Journal Title

      Proc. 8th Int. Conf. X-ray Microscopy, IPAP Conference series 7

      Pages: 326-327

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Arsenic Distribution and Speciation in the Arsenic Hyperaccumulator Fern by Micro-XRF Imaging and Micro-XANES Analysis2006

    • Author(s)
      Ryoko Onuma, et al.
    • Journal Title

      Proc. 8th Int. Conf. X-ray Microscopy, IPAP Conference series 7

      Pages: 323-325

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cadmium Distribution in a Cadmium Hyperaccumulator Plant as Determined by Micro-XRF Imaging2006

    • Author(s)
      Akiko Hokura, et al.
    • Journal Title

      Proc. 8th Int. Conf. X-ray Microscopy, IPAP Conference series 7

      Pages: 321-322

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Distribution and XANES of Arsenic in Root of Hyperaccumulator Fern (Pteris vittata L.) Measured by μ-SR-XRF Analysis2006

    • Author(s)
      Nobuyuki Kitajima, et al.
    • Journal Title

      Kagaku To Seibutsu 44(12)

      Pages: 860-864

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 2-D X-ray Fluorescence Imaging of Cadmium Hyperaccumulating Plants by Using High-energy Synchrotron Radiation X-ray Microbeam2006

    • Author(s)
      Akiko Hokura
    • Journal Title

      CHEMISTRY LETTERS 35(11)

      Pages: 1246-1247

    • NAID

      10018404846

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 放射光蛍光X線分析を用いるヒ素高集積植物モエジマシダの根におけるヒ素の分布及び化学形態分析2006

    • Author(s)
      柏原輝彦
    • Journal Title

      分析化学 55(10)

      Pages: 743-748

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Arsenic Distribution and Speciation in the Arsenic Hyperaccumulator Fern by Micro-XRF Imaging and Micro-XANES Analysis2006

    • Author(s)
      Ryoko Onuma
    • Journal Title

      Proc. 8th Int. Conf. X-ray Microscopy, IPAP Conference series 7

      Pages: 326-327

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cadmium Distribution in a Cadmium Hyperaccumulator Plant as Determined by Micro-XRF Imaging2006

    • Author(s)
      Akiko Hokura
    • Journal Title

      Proc. 8th Int. Conf. X-ray Microscopy, IPAP Conference series 7

      Pages: 323-325

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Distribution and XANES of Arsenic in Root of Hyperaccumulator Fern (Pteris vittata L.) Measured by μ-SR-XRF Analysis2006

    • Author(s)
      Nobuyuki Kitajima
    • Journal Title

      Proc. 8th Int. Conf. X-ray Microscopy, IPAP Conference series 7

      Pages: 321-322

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 放射光X線マイクロビームを用いた重金属蓄積植物の蛍光X線イメージング2006

    • Author(s)
      小沼亮子
    • Journal Title

      化学と生物 44(12)

      Pages: 860-864

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Arsenic distribution and speciation in an arsenic hyperaccumulator fern by X-ray spectrometry utilizing a synchrotron radiation source2006

    • Author(s)
      A.Hokura, R.Onuma, Y.Terada, N.Kitajima, T.Abe, H.Saito, S.Yoshida, I.Nakai
    • Journal Title

      Journal of Analytical Atomic Spectrometry 21

      Pages: 321-328

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cd Kα線を用いた蛍光X線分析法による玄米中sub-ppmレベルのCdの迅速定量2005

    • Author(s)
      永山裕之, 小沼亮子, 保倉明子, 中井 泉, 松田賢士, 水平 学, 赤井孝夫
    • Journal Title

      X線分析の進歩 36

      Pages: 235-247

    • NAID

      40006919961

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 放射光マイクロビームを用いた蛍光X線分析とXAFSによるモエジマシダのヒ素蓄積機構の研究2005

    • Author(s)
      保倉明子, 中井 泉
    • Journal Title

      ぶんせき 11

      Pages: 622-625

    • NAID

      10016772365

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 分析科学分野における放斜光結晶学-微小部分析,微量分析の極限にむけて2005

    • Author(s)
      中井 泉
    • Journal Title

      こんなことができる放斜光(7) 日本結晶学会誌 47

      Pages: 305-315

    • Related Report
      2005 Annual Research Report
  • [Journal Article] X-ray fluorescence imaging for Heavy metal Hyperaccumulator plants by using Synchrotron radiation X-ray micro beam

    • Author(s)
      Ryoko Onuma, et al.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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