| Project/Area Number |
17350087
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | Konan University |
|---|
Principal Investigator |
SUGIMOTO Naoki Konan University, Faculty of Science and Engineering, Professor (60206430)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKANO Shu-ichi Konan University, FIBER, Assistant Professor (70340908)
MIYOSHI Daisuke Konan University, FIBER, Assistant Professor (50388758)
KOMOTO Kazuya Konan University, FIBER, Assistant Professor (60388759)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥15,490,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥690,000)
Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2006: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2005: ¥9,900,000 (Direct Cost: ¥9,900,000)
|
|---|
| Keywords | Molecular crowding / Polyethylene glycol / Non-aqueous solution / Thermodynamics / Molecular interaction / Conformational change / Functional nucleotide / PCR / リボザイム / RNA加水分解 / 人工DNA / DNA高次構造 / 二次構造変化 / 四重鎖構造 / DNAポリメラーゼ / 論理素子 / 分子環境応答性 / 分子センシング / DNA / Watson-Crick / Hoogsteen / テロメア配列 |
|---|
| Research Abstract |
The purposes of this study are construction of a database for nucleotide interactions and development of functional nucleotides that can be applied for a molecular sensing in the non-aqueous media such as in a cell and on a sensor surface. We found the DNA hybridization energy on a gold nanoparticle differed from that obtained in a homogeneous aqueous solution. A model system that mimics the molecular crowding environment supposedly occurring in a cell and on a gold nanoparticle was constructed by using PEG (polyethylene glycol) and other cosolutes. A large amount of cosolutes added in an aqueous solution substantially decreases the water activity and large-sized cosolutes generate large excluded volume effect. Investigations of oligonucleotide structures and their thermodynamic parameters in the cosolute-containing solutions revealed that the molecular crowding by PEG or other cosolute decreased the stability of Watson-Crick base pairs but stabilized the triplex, G-quadruplex, and lef
… More
t-handed duplex (Z-form) structures. To understand the molecular crowding effect, we analyzed the thermodynamic and kinetic parameters and obtained the notations that water molecules participating in a nucleotide structure formation significantly influence the thermodynamic stability and the kinetics under the molecular crowding conditions. According to the nucleotide interaction data, we developed DNA molecules that changed the conformation when binding to polyamines or basic peptides, and those formed a DNA-wire by transferring into the molecular crowding media. Moreover, we constructed the DNA molecules with a logic gate function that responded to the molecular environment. We also demonstrated that the molecular crowding effect was used for up-or down-regulation of an enzyme activity and an efficient PCR amplification of a GC-rich DNA sequence. We also successfully synthesized several types of functional nucleotides. The researches that focus on the molecular crowding are very important from the standpoint of medical and engineering aspects. Less
|
