Study on Patterned Surface by Negative Ion Implantation and Neuron Network by Nerve Cell for Mutual Communication

• Project/Area Number: 17360021
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thin film/Surface and interfacial physical properties
• Research Institution: Kyoto University
• Principal Investigator: TSUJI Hiroshi Kyoto University, Graduate School of Engineering, Assistant Professor (20127103)
• Co-Investigator(Kenkyū-buntansha): ISHIKAWA Junzo Kyoto University, Graduate School of Engineering, Professor (80026278) ; GOTOH Yasuhito Kyoto University, Graduate School of Engineering, Associate Professor (00225666)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥16,320,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥1,020,000); Fiscal Year 2007: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2006: ¥6,200,000 (Direct Cost: ¥6,200,000); Fiscal Year 2005: ¥5,700,000 (Direct Cost: ¥5,700,000)
• Keywords: Negative ion implantation / Surface modification / Biocompatibility / Nerve cell / Align adhesion of cell / Stem cell / Mesenchymal stem cell / Neuron network / パターン化細胞接着制御 / 脳神経細胞 / 体性幹細胞 / 間葉幹細胞 / 分化誘導 / 人為的神経回路網 / 親水性 / 神経細胞接着 / 神経突起伸展 / 人為的神経回路 / 双方向情報伝達 / 神経刺激 / バイオインターフェイス

Research Abstract

1. Self-Adhesion of Mesenchymal Stem Cells on Polymeric Surface Patterned by Negative-Ion Implantation Polystyrene films on glass substrate and silicone rubber sheets were implanted with carbon negative ions at energy in 5-20 keV through a pattern mask which had many slits of 50 μm in width and 5 mm in length. After culturing mesenchymal stem cells derived from rat bone mallow on the samples, we obtained the spontaneous cell-adhesion on the modified region along the implanted pattern The suitable conditions for the self alignment was 3x10^<14> ions/cm^2 for the polystyrene and 1-3 x10^<15> ions/cm^2 for silicone rubber in the implantation energy of 5-20 keV. The reason for the large requirement in dose for silicone rubber is considered to be do to the stronger bonding of Si-O in siloxan main chain in comparison to C-C of polystyrene. Thus, we accomplished to make self alignment of mesenchymal stem cells on polymeric surface by using pattern implantation of carbon negative ions.
2. Neuro n Network Formation from Mesenchymal Stem Cell with Keeping Pattern Adhesion We induced differentiation of mesenchymal stem cells aligned on the carbon-negative-ion implanted area into neurons by using a method with beta-mercaptoethanol. After culturing mesenchymal stem cells in the induction medium for 180 min, all of cells differentiated into neuron-like cells with a round cell body and long filament. The neuron-like cells kept their adhesion position. Therefore, the cell pattern was reserved after the differentiation. The differentiated cells showed neuron specific enolase. This means that differentiated cells were nerve cells and that we can make nerve network with desired pattern by using negative-ion pattern implantation. As for stimulation by an electric pulse, the electrodes equipped under the polystyrene film were melted due to pin holes in the film. By this reason, we could not investigate about signal transfer between the differentiated cells.
3. Neuron Network Formation with Brain Cells by using Negative Ion Implantation Brain cells requires a special substrate for their adhesion on the surface such as poly-Lysine Therefore, we coated poly-D-lysine on the polystyrene, and then, implanted carbon negative ions on the sample for modified surface layer. By this degradation of poly-D-lysine, the neurons of rat embryonic cortex adhered on the unimplanted region. Thus, we accomplished the pattering of brain cells by the degradation method using negative ion implantation.

Research Products

• [Journal Article] Modification Limit Width of Carbon Negative-Ion Implantation to Polystyrene Surface for Nerve-Cell Adhesion and Neurite Outgrowth (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Journal Title: Transactions of Materials Research Society of Japan 32 Pages: 917-920
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Mesenchymal Stem Cell Attachment Properties on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): P. Sommani, H. Tsuji, H. Sato, M. Hattori, T. Yamada, Y. Gotoh and J. Ishikawa
  • Journal Title: Transactions of Materials Research Society of Japan 32 Pages: 921-924
  • NAID: 130007922967
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Protein Adsorption Properties on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): P. Sommani, H. Tsuji, H. Sato, M. Hattori, T. Yamada, Y. Gotoh and J. Ishikawa
  • Journal Title: Transactions of Materials Research Society of Japan 32 Pages: 925-928
  • NAID: 130007923034
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Nerve-Cell Attachment Properties of Polystyrene and Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Journal Title: Surface and Coatings Technology 201 Pages: 8123-8126
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Minimum Line Width of Ion Beam-Modified Polystyrene by Negative Carbon Ions for Nerve-Cell Attachment and Neurite Extension (2007)
  • Author(s): P. Sommani, H. Tsuji, H. Sato, M. Hattori, T. Yamada, Y. Gotoh and J. Ishikawa
  • Journal Title: Nuclear Instruments and Methods in Physics Research B 257 Pages: 118-121
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 炭素負イオン注入処理を施した高分子材料上におけるタンパク質吸着性の評価 (2007)
  • Author(s): 山田哲也、辻 博司、服部光高、ソムマニ・ピヤヌット、佐藤弘子、後藤康仁、石川順三
  • Journal Title: 真空 50 Pages: 579-582
  • NAID: 10019835925
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Enhanced Adhesion Properties of Mesenchymal Stem Cell on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Journal Title: Proceedings of the 3rd WACBE World Congress on Bioengineering(WACBE 2007) Conf102a115
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Modification Limit Width of Carbon Negative-Ion Implantation to Polystyrene Surface for Nerve-Cell Adhesion and Neurite Outgrowth (2007)
  • Author(s): H., Tsuji, P., Sommani, M., Hattori, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Journal Title: Transactions of Materials Research Society of Japan Vol.32 Pages: 917-920
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mesenchymal Stem Cell Attachment Properties on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): P., Sommani, H., Tsuji, H., Sato, M., Hattori, T., Yamada, Y., Gotoh, J., Ishikawa
  • Journal Title: Transactions of Materials Research Society of Japan Vol.32 Pages: 921-924
  • NAID: 130007922967
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Protein Adsorption Properties on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): P., Sommani, H., Tsuji, H., Sato, M., Hattori, T., Yamada, Y., Gotoh, J., Ishikawa
  • Journal Title: Transactions of Materials Research Society of Japan Vol.32 Pages: 925-928
  • NAID: 130007923034
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Nerve-Cell Attachment Properties of Polystyrene and Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H., Tsuji, P., Sommani, M., Hattori, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Journal Title: Surface and Coatings Technology Vol.201 Pages: 8123-8126
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Minimum Line Width of Ion Beam-Modified Polystyrene by Negative Carbon Ions for Nerve-Cell Attachment and Neurite Extension (2007)
  • Author(s): P., Sommani, H., Tsuji, H., Sato, M., Hattori, T., Yamada, Y., Gotoh, J., Ishikawa
  • Journal Title: Nuclear Instruments and Methods in Physics Research B Vol.257 Pages: 118-121
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evaluation of Absorption Properties of Protein on Polymeric Surface Modified by Carbon Negative-Ion Implantation (in Japanese) (2007)
  • Author(s): T., Yamada, H., Tsuji, M., Hattori, P., Sommani, H., Sato, Y., Gotoh, J., Ishikawa
  • Journal Title: Journal of Vacuum Society of Japan ("Shin-Kuu") Vo.50 Pages: 579-582
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enhanced Adhesion Properties of Mesenchymal Stem Cell on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H., Tsuji, P., Sommani, M., Hattori, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Journal Title: Proceedings of the 3rd WACBE World Congress on Bioengineering, WACBE 2007 No.Conf102a115 Pages: 761-764
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enhanced Adhesion Properties of Mesenchymal Stem Cell on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Journal Title: Proceedings of the 3rd WACBE World Congress on Bioengineering (WACBE 2007) Conf102a115
• [Journal Article] Neurite Outgrowth Properties on Spin-coated Polystyrene Modified by Carbon Negative-ion Implantation (2006)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, T.Kitamura, M.Hattori, Y.Gotoh, J.Ishikawa
  • Journal Title: Transactions of the Materials Research Society of Japan 31・3 Pages: 669-672
• [Journal Article] Nerve Cell Attachment Properties on Spin-coated Polystyrene Modified by Carbon Negative-ion Implantation (2006)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, T.Kitamura, M.Hattori, Y.Gotoh, J.Ishikawa
  • Journal Title: Transactions of the Materials Research Society of Japan 31・3 Pages: 673-676
• [Journal Article] Basic Fibrobrast Growth Factor Promotes Nerve Regeneration in a C-Ion Implantated Silicone Chamber (2006)
  • Author(s): R.Ikeguchi, R.Kakinoki, T.Matsumoto, T.Yamakawa, K.Nakayama, Y.Morimoto, H.Tsuji, J.Ishikawa, T.Nakamura
  • Journal Title: Brain Research 1090 Pages: 51-57
• [Journal Article] Immobilization of extracellular matrix on polymeric materials by carbon-negative-ion implantation (2005)
  • Author(s): H.Tsuji
  • Journal Title: Nuclear Instruments and Methods in Physics Research B B237 Pages: 459-464
• [Presentation] Avidin Pattering on Polystyrene Surface by Using Carbon Negative-Ion Implantation (2008)
  • Author(s): Piyanuch Sommani、辻 博司、山田哲也、小嶌裕之、後藤康仁、石川順三
  • Organizer: 2008年春季 第55回応用物理学関係連合講演会
  • Place of Presentation: 日本大学理工学部、船橋、日本
  • Year and Date: 2008-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 炭素負イオン注入処理によるシリコーンラバー上での間葉系幹細胞の接着限界幅 (2008)
  • Author(s): 小嶌裕之、辻 博司、ピヤヌット・ソムマニ、山田哲也、後藤康仁、石川順三
  • Organizer: 2008年春季 第55回応用物理学関係連合講演会
  • Place of Presentation: 日本大学理工学部、船橋、日本
  • Year and Date: 2008-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Avidin Pattering on Polystyrene Surface by Using Carbon Negative-Ion Implantation (2008)
  • Author(s): Piyanuch, Sommani, H., Tsuji, T., Yamada, H., Kojima, Y., Gotoh, J., Ishikawa
  • Organizer: The 55th Spring Meeting, 2008, The Japan Society of Applied Physics and Related Societies
  • Place of Presentation: Nihon University, Funahashi, Japan
  • Year and Date: 2008-03-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Minimum Line Width for Cell Adhesion of Mesenchymal Stem Cells on Silicone Rubber Surface by Carbon Negative-Ion Implantation (2008)
  • Author(s): H., Kojima, H., Tsuji, P., Sommani, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Organizer: The 55th Spring Meeting, 2008, The Japan Society of Applied Physics and Related Societies
  • Place of Presentation: Nihon University, Funahashi, Japan
  • Year and Date: 2008-03-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Avidin Pattering on Polystyrene Surface by Using Carbon Negative-Ion Implantation (2008)
  • Author(s): Piyanuch Sommani、辻 博司、山田哲也、小嶌裕之、後藤康仁、石川順三
  • Organizer: 2008年春季第55回応用物理学関係連合講演会(平成20年3月27〜30日)
  • Place of Presentation: 日本大学理工学部、船橋、日本
  • Year and Date: 2008-03-29
• [Presentation] 炭素負イオン注入処理によるシリコーンラバー上での間葉系幹細胞の接着限界幅 (2008)
  • Author(s): 小嶌裕之、辻 博司、ピヤヌット・ソムマニ、山田哲也、後藤康仁、石川順三
  • Organizer: 2008年春季第55回応用物理学関係連合講演会(平成20年3月27〜30日)
  • Place of Presentation: 日本大学理工学部、船橋、日本
  • Year and Date: 2008-03-29
• [Presentation] Adhesion Patterning of Mesenchymal Stem Cells on Polymeric Surfaces by Carb on Negative-Ion Implantation through a Mask and Their Differentiation into Neurons with Keeping Cell Alignment (2008)
  • Author(s): Hiroshi Tsuji
  • Organizer: Special Seminar of Max Planck Institute on Membrane and Neurophysics
  • Place of Presentation: Max Planck Institute for Biochemistry, Martinsreid, Germany
  • Year and Date: 2008-03-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Adhesion Patterning of Mesenchymal Stem Cells on Polymeric Surfaces by Carbon Negative-Ion Implantation through a Mask and Their Differentiation into Neurons with Keeping Cell Alignment (2008)
  • Author(s): Hiroshi, Tsuji
  • Organizer: Neurophysics Seminar in Max Planck Institute for Biochemistry
  • Place of Presentation: Max Planck Institute, Martinsreid, Munich, Germany
  • Year and Date: 2008-03-06
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Adhesion Patterning of Mesenchymal Stem Cells on Polymeric Surfaces by Carbon Negative-Ion Implantation through a Mask and Their Differentiation into Neurons with Keeping Cell Alignment (2008)
  • Author(s): Hiroshi Tsuji
  • Organizer: Special Seminor of Max Planck Institute on Membrane and Neurophysis (March 6, 2008)
  • Place of Presentation: Max Planck Institute for Biochemistry, Martinsreid, Germany
  • Year and Date: 2008-03-06
• [Presentation] Negative-Ion Sources and Negative Ion Beam Applications-Adhesion Patterning of Mesenchymal Stem Cells on Polymeric Surfaces by Carbon Negative-Ion Implantation through a Pattern Mask and Their Differentiation into Neuron with Keeping Cell Alignment (2008)
  • Author(s): Hiroshi Tsuji,
  • Organizer: Special Seminar of Labnoratoire des Solides Irradies in Ecole Polytechnique,
  • Place of Presentation: Ecole Polytechnque, Palaiseau, Feance
  • Year and Date: 2008-03-03
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Negative-Ion Sources and Negative Ion Beam Applications - Adhesion Patterning of Mesenchymal Stem Cells on Polymeric Surfaces by Carbon Negative-Ion Implantation through a Pattern Mask and Their Differentiation into Neuron with Keeping Cell Alignment (2008)
  • Author(s): Hiroshi, Tsuji
  • Organizer: Special Seminar of Labnoratoire des Solides Irradiess
  • Place of Presentation: Ecole Polytechnique, Palasiseau, France
  • Year and Date: 2008-03-03
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Pattern Arrangement of Nerve Cells Differentiated from Mesenchymal Stem Cells Selectively Adhered on Polystyrene Surface Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hatori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: International Academic Symposium of Materials Research Society of Japan(MRS-J2007)
  • Place of Presentation: Nihon University, Tokyo, Japan
  • Year and Date: 2007-12-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pattern Arrangement of Nerve Cells Differentiated from Mesenchymal Stem Cells Selectively Adhered on Polystyrene Surface Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H., Tsuji, P., Sommani, M., Hattori, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Organizer: International Academic Symposium of Materials Research Society of Japan (MRS-J2007)
  • Place of Presentation: Nihon University, Tokyo, Japan
  • Year and Date: 2007-12-09
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Pattern Arrangement of Nerve Cells Differentiated from Mesenchymal Stem Cells Selectively Adhered on Polystyrene Surface Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: International Academic Symposium of Materials Research Society of Japan
  • Place of Presentation: Nihon University, Tokyo, Japan
  • Year and Date: 2007-12-09
• [Presentation] Patterning of Neuron Adhesion from Differentiation of Mesenchymal Stem Cells on Silicone Rubber Modified by Carbon Negative-lon Implantation (2007)
  • Author(s): P. Sommani, H. Tsuji, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: International Academic Symposium of Materials Research Society of Japan(MRS-J2007)
  • Place of Presentation: Nihon University, Tokyo, Japan
  • Year and Date: 2007-12-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Patterning of Neuron Adhesion from Differentiation of Mesenchymal Stem Cells on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): P., Sommani, H., Tsuji, M., Hattori, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Organizer: International Academic Symposium of Materials Research Society of Japan (MRS-J2007)
  • Place of Presentation: Nihon University, Tokyo, Japan
  • Year and Date: 2007-12-08
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Patterning of Neuron Adhesion from Differentiation of Mesenchymal Stem Cells on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: International Academic Symposium of Materials Research Society of Japan
  • Place of Presentation: Nihon University, Tokyo, Japan
  • Year and Date: 2007-12-08
• [Presentation] 炭素負イオン注入によるポリスチレン上での間葉幹細胞のパターン接着と神経細胞への分化誘導 (2007)
  • Author(s): 辻 博司、ピヤヌット・ソムマニ、山田哲也、小嶌裕之、佐藤弘子、後藤康仁、石川順三
  • Organizer: 第48回真空に関する連合講演会
  • Place of Presentation: 学習院大学、東京、日本
  • Year and Date: 2007-11-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 炭素負イオン注入によるシリコーンラバー上での間葉幹細胞のパターン接着 (2007)
  • Author(s): 小嶌裕之、辻 博司、ピヤヌット・ソムマニ、山田哲也、佐藤弘子、後藤康仁、石川順三
  • Organizer: 第48回真空に関する連合講演会
  • Place of Presentation: 学習院大学、東京、日本
  • Year and Date: 2007-11-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pattern Adhesion of Mesenchymal Stem Cells on Polystyrene Surface by using Carbon Negative-Ion Implantation (2007)
  • Author(s): H., Tsuji, P., Sommani, T., Yamada, H., Kojima, H., Sato, Y., Gotoh, J., Ishikawa
  • Organizer: The 47th Annual Meeting of The Vacuum Society of Japan
  • Place of Presentation: Gakushuin University, Tokyo, Japan
  • Year and Date: 2007-11-15
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Pattern Adhesion of Mesenchymal Stem Cells on Silicone Rubber Surface by Carbon Negative-Ion Implantation (2007)
  • Author(s): H., Kojima, H., Tsuji, P., Sommani, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Organizer: The 47th Annual Meeting of The Vacuum Society of Japan
  • Place of Presentation: Gakushuin University, Tokyo, Japan
  • Year and Date: 2007-11-15
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 炭素負イオン注入によるポリスチレン上での間葉幹細胞のパターン接着と神経細胞への分化誘導 (2007)
  • Author(s): 辻 博司、ピヤヌット・ソムマニ、山田哲也、小嶌裕之、佐藤弘子、後藤康仁、石川順三
  • Organizer: 第48回真空に関する連合講演会(平成19年11月14〜16日)
  • Place of Presentation: 学習院大学、東京、日本
  • Year and Date: 2007-11-15
• [Presentation] 炭素負イオン注入によるシリコーンラバー上での間葉幹細胞のパターン接着 (2007)
  • Author(s): 小嶌裕之、辻 博司、ピヤヌット・ソムマニ、山田哲也、佐藤弘子、後藤康仁、石川順三
  • Organizer: 第48回真空に関する連合講演会(平成19年11月14〜16日)
  • Place of Presentation: 学習院大学、東京、日本
  • Year and Date: 2007-11-15
• [Presentation] Negative Ion Implantation for Pattering Mesenchymal Stem Cell Adhesion on Silicone Rubber and Differentiation into Nerve Cells with Preserving Their Adhesion Pattern (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: The 15th International Conference on Surface Modification of Materials by Ion Beams(SMMIB2007)
  • Place of Presentation: Tata Institute of Fundamental Research, Mumbai, India
  • Year and Date: 2007-10-02
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Negative Ion Implantation for Pattering Mesenchymal Stem Cell Adhesion on Silicone Rubber and Differentiation into Nerve Cells with Preserving Their Adhesion Pattern (2007)
  • Author(s): H., Tsuji, P., Sommani, M., Hattori, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Organizer: The 15th International Conference on Surface Modification of Materials by Ion Beams (SMMIB2007)
  • Place of Presentation: Tata Institute of Fundamental Research, Mumbai India
  • Year and Date: 2007-10-02
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Negative Ion Implantation for Pattering Mesenchymal Stem Cell Adhesion on Silicone Rubber and Differentiation into Nerve Cells with Preserving Their Adhesion Pattern (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: The 15th International Conference on Surface Modification of Materials by Ion Beams (SMMIB2007, Sept. 30-Oct. 5, Mumbai)
  • Place of Presentation: Tata Institute of Fundamen-tal Research, Mumbai, India
  • Year and Date: 2007-10-02
• [Presentation] Surface Modification by Negative-Ion Implantation (2007)
  • Author(s): J. Ishikawa, H. Tsuji, and Y. Gotoh
  • Organizer: The 15th International Conference on Surface Modification of Materials by Ion Beams(SMMIB2007)
  • Place of Presentation: Tata Institute of Fundamental Research, Mumbai, India
  • Year and Date: 2007-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Surface Modification by Negative-Ion Implantation (2007)
  • Author(s): J., Ishikawa, H., Tsuji, Y., Gotoh
  • Organizer: The 15th International Conference on Surface Modification of Materials by Ion Beams (SMMIB2007)
  • Place of Presentation: Tata Institute of Fundamental Research, Mumbai, India
  • Year and Date: 2007-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Surface Modification by Negative-Ion Implantation (2007)
  • Author(s): J. Ishikawa, H. Tsuji, and Y. Gotoh
  • Organizer: The 15th International Conference on Surface Modification of Materials by Ion Beams (SMMIB2007, Sept. 30-Oct. 5, Mumbai)
  • Place of Presentation: Tata Institute of Fundamen-tal Research, Mumbai, India
  • Year and Date: 2007-10-01
• [Presentation] Neuron Arrangement from Mesenchymal Stem Cells on Polymers Patterned by Negative-Ion Implantation (2007)
  • Author(s): Piyanuch Sommani、辻 博司、山田哲也、小嶌裕之、佐藤弘子、後藤康仁、石川順三
  • Organizer: 2007年秋季 第68回応用物理学会学術講演会
  • Place of Presentation: 北海道工業大学、札幌、日本
  • Year and Date: 2007-09-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Neuron Arrangement from Mesenchymal Stem Cells on Polymers Patterned by Negative-Ion Implantation (2007)
  • Author(s): P., Sommani, H., Tsuji, T., Yamada, H., Kojima, H., Sato, Y., Gotoh, J. Ishikavva
  • Organizer: The 68th Autumn Meeting, 2007, The Japan Society of Applied Physics
  • Place of Presentation: Hokkaido Institute of Technology, Sapporo, Japan
  • Year and Date: 2007-09-05
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Neuron Arrangement from Mesenchymal Stem Cells on Polymers Patterned by Negative-Ion Implantation (2007)
  • Author(s): Piyanuch Sommani、辻 博司、山田哲也、小嶌裕之、佐藤弘子、後藤康仁、石川順三
  • Organizer: 2007年秋季第68回応用物理学会学術講演会(平成19年9月4〜8日)
  • Place of Presentation: 北海道工業大学、札幌、日本
  • Year and Date: 2007-09-05
• [Presentation] 炭素負イオン注入処理によるシリコーンゴム表面のポリDリジン吸着性の向上 (2007)
  • Author(s): 辻 博司、小嶌裕之、ピヤヌット・ソムマニ、山田哲也、佐藤弘子、後藤康仁、石川順三
  • Organizer: 2007年秋季 第68回応用物理学会学術講演会
  • Place of Presentation: 北海道工業大学、札幌、日本
  • Year and Date: 2007-09-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 炭素負イオンパターン注入によるシリコーンラバー上での間葉幹細胞のパターン接着及び分化誘導 (2007)
  • Author(s): 山田哲也、辻 博司、小嶌裕之、ソムマニ・ピヤヌット、後藤康仁、石川順三
  • Organizer: 2007年秋季 第68回応用物理学会学術講演会
  • Place of Presentation: 北海道工業大学、札幌、日本
  • Year and Date: 2007-09-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Poly-D-Resin Absorption Property on Silicone Rubber Surface Improved by Carbon Negative-Ion Implantation (2007)
  • Author(s): H., Tsuji, H., Kojima, P., Sommani, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Organizer: The 68th Autumn Meeting, 2007, The Japan Society of Applied Physics
  • Place of Presentation: Hokkaido Institute of Technology, Sapporo, Japan
  • Year and Date: 2007-09-04
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Pattern Adhesion of Mesenchymal Stem Cells on Silicone Rubber Modified by Carbon Negative-Ion Implantation and Their Differentiation into Neurons (2007)
  • Author(s): T., Yamada, H., Tsuji, H., Kojima, P., Sommani, Y., Gotoh, J., Ishikawa
  • Organizer: The 68th Autumn Meeting, 2007, The Japan Society of Applied Physics
  • Place of Presentation: Hokkaido Institute of Technology, Sapporo, Japan
  • Year and Date: 2007-09-04
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 炭素負イオン注入処理によるシリコーンゴム表面のポリDリジン吸着性の向上 (2007)
  • Author(s): 辻 博司、小嶌裕之、ピヤヌット・ソムマニ、山田哲也、佐藤弘子、後藤康仁、石川順三
  • Organizer: 2007年秋季第68回応用物理学会学術講演会(平成19年9月4〜8日)
  • Place of Presentation: 北海道工業大学、札幌、日本
  • Year and Date: 2007-09-04
• [Presentation] 炭素負イオンパターン注入によるシリコーンラバー上での間葉幹細胞のパターン接着及び分化誘導 (2007)
  • Author(s): 山田哲也、辻 博司、小嶌裕之、ソムマニ・ピヤヌット、後藤康仁、石川順三
  • Organizer: 2007年秋季第68回応用物理学会学術講演会(平成19年9月4〜8日)
  • Place of Presentation: 北海道工業大学、札幌、日本
  • Year and Date: 2007-09-04
• [Presentation] Adhesion Pattering of Mesenchymal Stem Cells on Polystyrene Surface by Carbon Negative-Ion Implantation and Neuron Differentiation on the Position (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: The 14th International Conference on Radiation Effects on Insulators(REI-14)
  • Place of Presentation: Caen Basse Normandie University, Caen, France
  • Year and Date: 2007-08-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Adhesion Pattering of Mesenchymal Stem Cells on Polystyrene Surface by Carbon Negative-Ion Implantation and Neuron Differentiation onthe Position (2007)
  • Author(s): H., Tsuji, P., Sommani, M., Hattori, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Organizer: The 14th International Conference on Radiation Effects on Insulators (REI-14)
  • Place of Presentation: Caen Basse Normandie University, Caen, France
  • Year and Date: 2007-08-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Adhesion Pattering of Mesenchymal Stem Cells on Polystyrene Surface by Carbon Negative-Ion Implantation and Neuron Differentiation on the Position (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: The 14th International Conference on Radiation Effects on Insulators (REI-14, August 28-September 1, Caen, France)
  • Place of Presentation: Caen Basse Normandie Univesity, Caen, France
  • Year and Date: 2007-08-30
• [Presentation] Adhesion Properties of Mesenchymal Stem Cell on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: The 3rd WACBE World Congress on Bio-Engineering(WACBE2007)
  • Place of Presentation: The Twin Tower Hotel, Bangkok, Thailand
  • Year and Date: 2007-07-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Fine Pattern Immobilization of Nerve Cells and Neurite Extension on Polystyrene Films Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): P. Sommani, H. Tsuji, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: The 3rd WACBE World Congress on Bio-Engineering(WACBE2007)
  • Place of Presentation: The Twin Tower Hotel, Bangkok, Thailand
  • Year and Date: 2007-07-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Adhesion Properties of Mesenchymal Stem Cell on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H., Tsuji, P., Sommani, M., Hattori, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Organizer: The 3rd WACBE World Congress on Bio-Engineering (WACBE2007)
  • Place of Presentation: The TwinTower Hotel, Bangkok, Thailand
  • Year and Date: 2007-07-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Fine Pattern Immobilization of Nerve Cells and Neurite Extension on Polystyrene Films Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): P., Sommani, H., Tsuji, M., Hattori, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Organizer: The 3rd WACBE World Congress on Bio-Engineering (WACBE2007)
  • Place of Presentation: The Twin Tower Hotel. Bangkok,Thailand
  • Year and Date: 2007-07-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Adhesion Properties of Mesenchymal Stem Cell on Silicone Rubber Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: The 3rd WACBE World Congress on Bio-Engineering (WACBE2007, July 8-10, Bangkok, Thailand)
  • Place of Presentation: The Twin Tower Hotel, Bangkok, Thailand
  • Year and Date: 2007-07-10
• [Presentation] Fine Pattern Immobilization of Nerve Cells and Neurite Extension on Polystyrene Films Modified by Carbon Negative-Ion Implantation (2007)
  • Author(s): P. Sommani, H. Tsuji, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: The 3rd WACBE World Congress on Bio-Engineering (WACBE2007, July 8-10, Bangkok, Thailand)
  • Place of Presentation: The Twin Tower Hotel, Bangkok, Thailand
  • Year and Date: 2007-07-10
• [Presentation] Adhesion Control of Mesenchymal Stem Cells on Polystyrene Surface by Carbon Negative-Ion Implantation through Pattering Mask (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: The 17th International Vacuum Congress(IVC-17)
  • Place of Presentation: International Fairs, Stockholm, Sweden
  • Year and Date: 2007-07-02
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Adhesion Control of Mesenchymal Stem Cells on Polystyrene Surface by Carbon Negative-Ion Implantation through Pattering Mask (2007)
  • Author(s): H., Tsuji, P., Sommani, M., Hattori, T., Yamada, H., Sato, Y., Gotoh, J., Ishikawa
  • Organizer: The 17th International Vacuum Congress (IVC-17)
  • Place of Presentation: International Fairs, Stockholm, Sweden
  • Year and Date: 2007-07-02
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Adhesion Control of Mesenchymal Stem Cells on Polystyrene Surface by Carbon Negative-Ion Implantation through Pattering Mask (2007)
  • Author(s): H. Tsuji, P. Sommani, M. Hattori, T. Yamada, H. Sato, Y. Gotoh and J. Ishikawa
  • Organizer: The 17th International Vacuum Congress (IVC-17, July1-6, Stockholm, Sweden)
  • Place of Presentation: International Fairs, Stockholm, Sweden
  • Year and Date: 2007-07-02