Investigation of the Mechanism of the Production and the Evaluation as a Cell Culture Support of Polymer Porous Medium with Controlled 3-Dimensional Structure
| Project/Area Number |
17360381
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Reaction engineering/Process system
|
|---|
| Research Institution | Yokohama National University |
|---|
Principal Investigator |
NISHI Kazuhiko Yokohama National University, Graduate School of Engineering, Associate Professor, 大学院工学研究院, 助教授 (20262412)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAMINOYAMA Meguru Yokohama National University, Graduate School of Engineering, Professor, 大学院工学研究院, 教授 (50233945)
MISUMI Ryuta Yokohama National University, Graduate School of Engineering, Assistant, 大学院工学研究院, 助手 (40334635)
WATANABE Masatoshi Yokohama National University, Graduate School of Engineering, Professor, 大学院工学研究院, 教授 (90273383)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥8,200,000 (Direct Cost: ¥8,200,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2005: ¥6,100,000 (Direct Cost: ¥6,100,000)
|
|---|
| Keywords | polymerization / supercritical fluid / reaction, separation engineering / cell, cellular tissue / chemical engineering |
|---|
| Research Abstract |
In this study, the production of the polymer porous medium by polymerization in supercritical carbon dioxide was investigated.
1.Control of 3-dimensional structure of the polymer porous medium
The cross linked polymerization of styrene and acrylics nitril was performed in supercritical carbon dioxide, and the control method of the 3-dimensional structure of the polymer porous medium was investigated. Polymerization was performed on various conditions of pressure, temperature and concentration. The relation between the polymerization conditions and the diameter of the porous medium, the diameter of a primary particle, specific surface area and the diameter distribution of fine pores was shown.
2.Mechanism of the polymer porous medium production
It is guessed that the solubility of the polymer in polymerizing field influences on the mechanism of polymer porous medium production. Based on time until polymer deposits out of polymerization solution, the solubility of polymer was shown quantitat
… More
ively, and the influence polymerization condition on structure was explained.
3.Production of the polymer porous medium using stirred type supercritical polymerization reactor
The industrial production of the polymer porous medium using stirred type supercritical polymerization reactor was considered. The procedure which produces a styrene porous medium was established. The influence of the impeller rotations number on the rate of polymerization and structure was shown.
4.Molding to polymer porous medium of the polymer particles and surface treatment, washing of the porous medium
The method of molding polymer particles on the porous medium by treating with supercritical carbon dioxide was discussed. Furthermore, the surface treatment and washing of the polymer porous medium using supercritical carbon dioxide were studied.
5.Cultivation of human prostatic cancer cell using polymer porous medium
The human prostatic cancer cell culture using the polystyrene and the poly acrylics nitrile porous medium as a culture support was performed. In all porous mediums, the cell was increasing to the inside of the porous medium, and it was confirmed that the polymer porous medium in this study can use it as a cell a culture support. Less
|
Report
(3 results)
Research Products
(3 results)
