Development of oviduct specific promoter system for transgenic chicken bioreactors
| Project/Area Number |
17360396
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KAMIHIRA Masamichi Kyushu University, Faculty of Engineering, Professor, 大学院工学研究院, 教授 (40202022)
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| Co-Investigator(Kenkyū-buntansha) |
IIJIMA Shinji Nagoya University, Graduate School of Engineering, Professor, 大学院工学研究科, 教授 (00168056)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 2006: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 2005: ¥7,900,000 (Direct Cost: ¥7,900,000)
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| Keywords | Bioengineering / Biotechnology / Expression control / Applied animals / Viral vector / Transgenic chicken / Promoter |
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| Research Abstract |
To establish a transgenic bioreactor system using chickens for production of recombinant proteins into their eggs, a specific-expression system of target protein was developed. Although it is necessary to construct an oviduct-specific expression system, whole control region of an oviduct-specific promoter such as ovalbumin promoter cannot be introduced into a retroviral vector for gene delivery, since retroviral vectors have size limitation of an insert gene. Thus, a synthetic promoter system, in which a minimum control region for the tissue-specific expression and an artificial induction system for high-level expression were combined, was designed. First of all, tissue-specific control regions of ovalbumin promoter were divided into DNase hyper-sensitive regions according to a previous study, and an effective region for oviduct-specific expression was determined. During this study, it was found that a transcription factor, YY1 binds to a negative-control region of ovalbumin promoter. The synthetic promoter system, firstly designed in this study, including a positive feed-back of expression of the transactivator could induce a target protein with high level. However, leak expression had occurred and modification of the synthetic promoter system was required. By replacing the minimum promoter included in the synthetic promoter with a small region of ovalbumin promoter including TATA box and proximal region, leak expression could be minimized. These results indicate that the synthetic promoter construct for oviduct-specific and high-level expression can be used for transgenic chicken bioreactors..
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Report
(3 results)
Research Products
(7 results)
