Regulation of cell differentiation and stimulation on D-aglucse displayed dendrimer surface
| Project/Area Number |
17360398
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
KINO-OKA Masahrio Osaka University, Chemical Engineering, Associate Professor (40234314)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAWASE Masaya Osaka-Otani University, Pharmacy, Professor (90224782)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥11,660,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥660,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2005: ¥6,600,000 (Direct Cost: ¥6,600,000)
|
|---|
| Keywords | Biotechnology and Bionics / Cell and tissue / Regenerative medicine / Desien of culture surface / regulation of cell differentiation / Chondrocyte cell / Epithelial cell / D-glucose display |
|---|
| Research Abstract |
Adhesion of anchorage-dependent cells to underlying substrate is known to modulate a variety of cellular events such as signaling, gene expression and proteogenic changes. In particular, integrin bindings to cellular ligands results in functional consequences such as cell adhesion, shape change, and migration. that is crucial to cellular functions in several cell species. Thus, the development of these substrates for cell adhesion can provide new insights into cell biology as well as sophisticated methodology for controlling morphogenesis of cultured cells and tissues.
This project consisted of three parts; the design of culture surface and characterization, understanding of cell attachment and morphology on D-glucose-displayed surface as the fundamental study, and its application for the cell functions. The first part covered the surface design and characterization. The second part which contained the last two themes deals with the elucidation of mechanism of morphological changes based on glucose-displayed surface; morphological changes of epithelial cells on glucose-displayed surface, morphological regulation of chondrocytes, and subsequently, chondrogenic expression on glucose-displayed surface. In the last part, we proposed that new function arising from culture surface such as targetability and adhesion to glucose-displayed surface could introduce a novel signal-transduction system to be applied for tissue construction.
|
Report
(4 results)
Research Products
(37 results)
