Molecular dissection of regulatory mechanism that controls cell-type specific cell wall construction using XTH family of genes as a model system
| Project/Area Number |
17370012
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Tohoku University |
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Principal Investigator |
NISHITANI Kazuhiko Tohoku UnTversity., Graduate School of Life Sciences, Professor, 大学院生命科学研究科, 教授 (60164555)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOYAMA Ryusuke Tohoku University, Graduate School of Life Sciences, Research Associate, 大学院生命科学研究科, 講師 (90302083)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2006: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2005: ¥10,000,000 (Direct Cost: ¥10,000,000)
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| Keywords | plant / cell wall / morphogenesis / gene family / XTH / xyloglucan / Arabidopsis / vascular / ゲノム / 発現制御 / 発生・分化 / 遺伝子ファミリー / 根 / 維管束形成 / 生殖器官 |
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| Research Abstract |
Xyloglucan endotransglucosylases/hydrolases (XTHs) are a class of enzymes capable of catalyzing the molecular grafting between xyloglucans and/or the endotype hydrolysis of a xyloglucan molecule. They are encoded by 33 genes in Arabidopsis. Whereas recent studies have revealed temporally and spatially specific expression profiles for individual members of this family in plants, their biological roles are still to be clarified. To identify the role of each member of this gene family, we examined phenotypes of mutants in which each of the Arabidopsis XTH genes was disrupted.
Two loss-of-function mutants for the AtXTH27 gene, xth27-1 and xth27-2 exhibited short-shaped tracheary elements in tertiary veins, and reduced the number of tertiary veins in the first leaf. In mature rosette leaves of the mutant, yellow lesion-mimic spots were also observed. Upon genetic complementation by introducing the wild-type XTH27 gene into xth27-1 mutant plants, the number of tertiary veins was restored, and
… More
the lesions disappeared completely. Extensive expression of the pXTH27::GUS fusion gene was observed in immature tracheary elements in the rosette leaves. The highest level of AtXTH27 mRNA expression in the rosette leaves was observed during leaf expansion, when the tracheary elements were elongating. These findings indicate that AtXTH27 plays an essential role during the generation of tracheary elements in the rosette leaves of Arabidopsis.
The Arabidopsis thaliana XTH genes AtXTH17, 18, 19, and 20 are phylogenetically closely related to one another and are preferentially expressed in the roots. However, they exhibit different expression profiles within the root and respond to hormonal signals differently. To investigate their functions in root growth, we examined phenotypes of loss-of-function mutants for these genes using T-DNA insertion lines and RNAi plants. These functional analyses disclosed a principal role for the AtXTH18 gene in primary root elongation. Of the four XTH genes, AtXTH18 exhibits the highest level of mRNA expression. We also determined auxin-signaling pathways for these genes using a mutant with a defect in the AXR2/IAA7 gene and found that the expression of AtXTH19 in the elongation/maturation region of the root is under the control of the AXR2/IAA7 signaling pathway. Less
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Report
(3 results)
Research Products
(36 results)
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[Journal Article] An isoflavone conjugate-hydrolyzing β -glucosidase from the roots of soybean (Glycine max) seedlings. Purification, gene cloning, phylogenetics and cellular localization.2006
Author(s)
Suzuki, H., Takahashi.S., Watanabe.R., Fukushima, Y., Fujita, N., Noguch, A., Yokoyama.R., Nishitani, K., Nishino.T., Nakayama, T.
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Journal Title
J Biol Chem 281
Pages: 30251-30259
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Carbohydrate-binding module of a rice endo- β -1.4-glycanase, OsCel9A. expressed in auxin-induced lateral root primordial. is post-translationally truncated.2006
Author(s)
Yoshida, K., Imaizumi, N., Kanako, S., Kawagoe.Y., Tagiri.A., Tanaka, H., Nishitani, K., Komae.K.
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Journal Title
Plant Cell Physiol. 47
Pages: 1555-1571
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] An isoflavone conjugate-hydrolyzing β-glucosidase from the roots of soybean (Glycine max) seedlings. Purifiocation, gene cloning, phylogenetics and cellular localization.2006
Author(s)
Suzuki H, Takahashi S, Watanabe R, Fukushima Y, Fujita N, Noguch A, Yokoyama R, Nishitani K, Nishino T, Nakayama T.
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Journal Title
J Biol Chem 281
Pages: 30251-30259
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Carbohydrate-binding module of a rice endo-β-1,4-glycanase, OsCel9A expressed in auxin-induced lateral root primordial, is post-translationally truncated2006
Author(s)
Yoshida K, Imaizumi N, Kanako S, Kawagoe Y, Tagiri A, Tanaka H, Nishitani K, Komae K.
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Journal Title
Plant Cell Physiol. 47
Pages: 1555-1571
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Kappa-View : the web-based, plant metabolic pathway analysis tool for integration of transcript and metabolite data.2005
Author(s)
Tokimatsu, T., Sakurai, N., Suzuki, H., Ohta, H., Nishitani, K., Koyama, T., Umezawa, T., Misawa, N., Saito.K., Shibata, D.
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Journal Title
Plant Physiol. 138
Pages: 1289-1300
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Kappa-View : the web-based, plant metabolic pathway analysis tool for integration of transcript and metabolite data.2005
Author(s)
Tokimatsu, T., Sakurai, N., Suzuki, H., Ohta, H., Nishitani, K., Koyama, T., Umezawa, T., Misawa, N., Saito, K.Shibata, D.
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Journal Title
Plant Physiol. 138
Pages: 1289-1300
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Differential expression of AtXTH17, -18, -19 and -20 genes in Arabidopsis roots. Physiological roles in specification in cell wall construction2005
Author(s)
Vissenberg, K., Oyama, M., Osato, Y., Yokoyama, R., Verbelen, J-P, Nishitani, K.
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Journal Title
Plant Cell Physiol 46
Pages: 192-200
Related Report
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[Journal Article] AtXTH27 plays an essential role in cell wall modification during the development of tracheary elements2005
Author(s)
Matsui, A., Yokoyama, R., Ito, T., Seki, M., Shinozaki, K., Takahashi, T., Komeda, Y., Nishitani, K.
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Journal Title
Plant J 42
Pages: 525-534
Related Report
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[Journal Article] Kappa-View : the web-based, plant metabolic pathway analysis tool for integration of transcript and metabolite data2005
Author(s)
Tokimatsu, T., Sakurai, N., Suzuki, H., Ohta, H., Nishitani, K., Koyama, T., Umezawa, T., Misawa, N., Saito, K., Shibata, D
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Journal Title
Plant Physiol 138
Pages: 1289-1300
Related Report
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