Site-recognition of RNA editing in chloroplasts
| Project/Area Number |
17370020
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Nagoya City University |
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Principal Investigator |
SUGIURA Masahiro Nagoya City Univ., Graduate School of Natural Sciences, Professor Emeritus, 大学院システム自然科学研究科, 名誉教授 (80027044)
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| Co-Investigator(Kenkyū-buntansha) |
YUKAWA Yasushi Nagoya City Univ., Graduate School of Natural Sciences, Associate Professor, 大学院システム自然科学研究科, 助教授 (70381902)
NAKAMURA Masayuki Nagoya City Univ., Graduate School of Natural Sciences, Research Associate, 大学院システム自然科学研究科, 研究員 (60322145)
KURODA Hiroshi Nagoya City Univ., Graduate School of Natural Sciences, Research Associate, 大学院システム自然科学研究科, 研究員 (80381903)
森山 昭彦 名古屋市立大学, 大学院・システム自然科学研究科, 教授 (50145744)
小保方 潤一 名古屋大学, 遺伝子実験施設, 助教授 (50185667)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 2006: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 2005: ¥5,900,000 (Direct Cost: ¥5,900,000)
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| Keywords | tobacco / chloroplast / RNA editing / RNA editing site / ndhD / ndhF / cis-element / in vitro system |
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| Research Abstract |
1. RNA editing sites
(1) Two additional sites were identified in the ndhD mRNA from tobacco (N. tabacum) chloroplasts. The total number of sites is thus 38, the most so far found in higher plant chloroplasts.
(2) ACG to AUG RNA editing in ndhD mRNAs from tobacco progenitors (N sylvestris and N. tomentosiformis) was reexamined, and this editing in the latter species occurred depending on culture conditions.
2. Improvement of the in vitro RNA editing assay
A rapid and simple in vitro assay was established using fluorescent dideoxynucleotides.
3. Cis-elements for RNA editing
Using the above assay, editing activities of 19 sites in five tobacco ndh mRNA species were measured. A cis-element in the ndhB mRNA was defined to be 5 nucleotides (nt) from-6 to-10, whereas ndhF mRNAs have two separate cis-elements, 15 nt from-1 to-15 and 5 nt from-36 to-40.
4. Trans-factors
Using fuorescent mRNAs, a method to detect trans-factors for editing was tried.
5. Analysis of site-recognition factors
The 56 kD trans-factor for psbE mRNA editing was isolated, and its partial amino acid sequences were determined. No similar sequence was found in databases.
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Report
(3 results)
Research Products
(27 results)
