Molecular neurophysiology of neural mechanisms of taste reception in Drosophila
| Project/Area Number |
17370027
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Kyushu University |
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Principal Investigator |
TANIMURA Teiichi Kyushu University, Department of Biology, Graduate School of Sciences, Associate Professor (20142010)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Akira Kyushu University, Center for Research and Advancement in Higher Education, Research Professor (40229539)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥14,600,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥900,000)
Fiscal Year 2007: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2006: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2005: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | Drosophila / taste / electrop hysiolo gy / receptor / projection pattern / G-protein |
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| Research Abstract |
(1) We showed that a G-protein gamma subunit is partially required for sugar taste reception in Drosophila. The gene for the G-protein gamma subunit is expressed mainly in one of the gustatory receptor neurons. Behavioral and electrophysiological responses of the flies to sucrose were reduced by the targeted neural suppression of Gel gamma subunit-expressing cells utilizing Ga14/UAS system. We also demonstrated that responses to sugars were lowered in Gcl gamma null mutant.
(2) Insects have specialized gustatory cells responding to water. We found a Ga14 enhancer trap strain in which GAL4 is expressed in a single taste receptor cell in each sensillum on the labellum. The neural function of these cells was blocked by expressing shibire [ts1] and behavioral tests and electrophysiological recordings indicated that they are the water taste cells. We show that axons of the water receptor cells project to a specific region in the subesophageal ganglion.
(3) Sex pheromones are volatile or non-volatile in Drosophila. Volatile pheromones are sensed by olfactory neurons and we found that labellar Gr66a-expressing taste neurons respond to a male specific non-volatile pheromone, 7-tricosene. This was done by a newly developed tungsten electrode recording method. 7-Tricosene is probably sensed by tarsal taste sensilla during courtship and we observed projection patterns from Gr66a-expressing neurons in tarsi. By using a Flp-out technique, we generated mosaic flies in which only a few Gr66a-expressing neurons can be labeled and observed their projection patterns. Results showed that projection patterns of Gr66a-expressing neurons from sensilla that might sense the sex-pheromone and those from other sensilla that are involved in feeding behavior are different. We suggest that information from Gr66a-expressing neurons is sent to a different region depending on their related behavior.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] A functional genomics strategy reveals clockwork orange as a transcriptional regulator in the Drosophila circadian clock.2007
Author(s)
Matsumoto, A., Ukai-Tadenuma, M., Yamada, R.G., Houl, J., Uno, K.D., Kasukawa, T., Dauwalder, B., Itoh, TQ., Takahashi, T., Ueda, R., Hardin, PE., Tanimura, T. &, Ueda, H.R.
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Journal Title
Genes Dev 21
Pages: 1687-1700
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] An inhibitory sex pheromone tastes bitter for Drosophila males2007
Author(s)
Lacaille, F., Hiroi, M., Twele, R., Inoshita, T., Umemoto, D., Maniere, G., Marion-Poll, F., Ozaki, M., Francke, W., Everaerts, C., Tanimura, T. &., Ferveur, J.-F.
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Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Drosophila has two circadian clocks with different responsiveness to light2005
Author(s)
Yoshii, T., Funada, Y., Murata', M., Ibuki', T., Matsumoto, A., Tanimura, T., Tomioka
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Journal Title
Eur. J. Neurosci 22
Pages: 1176-1184
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Drosophila has two circadian clocks with different responsiveness to light.2005
Author(s)
Yoshii, T., Funada, Y., Murata M., Ibuki T., Matsumoto, A., Tanimura, T., Tomioka, K.
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Journal Title
European Journal of Neuroscience 22
Pages: 1176-1184
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[Presentation] Overlapping processing of sex pheromone and bitterness in Drosophila2006
Author(s)
Fabien, Lacaille, Makoto, Hiroi, Robert, Twele, Tsuyoshi, Inoshita, Daisuke, Umemoto, Gerard, Maniere, Frederic, Marion-Poll, Mamiko, Ozaki, Wittko, Francke, Matthew, Cobb, Claude, Everaerts, Teiichi, Tanimura, Jean-Francois, Ferveur
Organizer
17th conference of the European Chemoreception Research Organization
Place of Presentation
Granada, Spain
Description
「研究成果報告書概要(欧文)」より
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