| Project/Area Number |
17370054
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Yokohama National University |
|---|
Principal Investigator |
NAITO Akira Yokohama National University, Graduate School of Engineering, Professor (80172245)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Katsuyuki Yokohama National University, Institute for Molecular Science, Associate Professor (2005,2006) (00334631)
KAWAMURA Izuru Yokohama National University, Graduate School of Engineering, Research Associate (2007) (20452047)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥13,090,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥690,000)
Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥6,600,000 (Direct Cost: ¥6,600,000)
|
|---|
| Keywords | Human calcitonin / Glucagon / Amyloid fibril / Lipid bilayer / Clucmin / High resolution solid-state NMR / Electron microscope / Fibrillation inhibition / 線維形成組害 / カルシトニン / アルツハイマー病 / 脂質二重膜 / 2段階自己触媒反応 / CP-MAS / サケカルシトニン |
|---|
| Research Abstract |
Human calcitonin(hCT)is known as an amyloid forming peptide by taking two steps reaction mechanism such as homogeneous association to form nucleus(k_1)and autocatalytic elongation of the fibrils (k_2). In this study, first, intermediate of the fibril formation of human calcitonin was observed in HEPES buffer solution. We have further observed the transition processes from intermediate to protofibril in the HEPES solution. This finding suggests that a spherical shape of intermediate is commonly occurred in the process of amyloid fibril formation as is observed in the Aβ-amyloid peptide. Second, three types of fibril inhibition mechanisms for hCT were investigated. First, F16L and F19L-hCTs were prepared and the fibrillation kinetics was examined. It turned out that the k_2 was significantly reduced in this mutants. Second, it was revealed that a charged amino acid such as Asp can also inhibit the fibril formation of hCT. In this case, k_1 was significantly reduced, while k_2 was not reduced. Third, we found that polyphenol compound crucmin completely inhibited the fibril formation. Next, we have examined the fibril formation of glucagon in the presence of lipid bilayers to underastand the fibril formation processes in the living cell, since lucagon is the 29-residue peptide hormone and is known to form amyloid fibril under the acidic condition. In this case k_2 was decreased, while k_1 was increased as compared with the fibril formation in the acidic solution, indicating that lipids were affected to form amyloid fibril. It is of interest to note that the structure of glucagons fibril grown in the presence of lipid bilayer is different from that in the absence of lipid bilayers. Namely, N-terminal part remains α-helix in the fibril form, while α-helix changed to β-sheet in the fibril grown in the absence of lipid bilayers.
|
