Molecular Mechanism of Cell Wall Integrity Checkpoint

• Project/Area Number: 17370060
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: OHYA Yoshikazu The University of Tokyo, Graduate School of Frontier Sciences, Integrated Biosciences, Professor (20183767)
• Co-Investigator(Kenkyū-buntansha): SONOIKE Kintake The University of Tokyo, Graduate School of Frontier Sciences, Integrated Biosciences, Associate Professor (30226716)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥14,500,000 (Direct Cost: ¥14,500,000); Fiscal Year 2006: ¥7,300,000 (Direct Cost: ¥7,300,000); Fiscal Year 2005: ¥7,200,000 (Direct Cost: ¥7,200,000)
• Keywords: Cell cycle / checkpoint / dynactin / cell wall / cyclin / spindle / 細胞周期 / チェックポイント / ダイナクチン複合体 / 細胞内局在 / フォークヘッドタンパク質

Research Abstract

Cell wall remodeling and cell morphogenesis are tightly coordinated with progression of the cell cycle in many organisms. In the budding yeast, Saccharomyces cerevisiae, a major cell wall component, 1,3-β-glucan is synthesized at the budding site in the early stage of the cell cycle, and constructed in the primary septum before cytokinesis. Yeast possesses both forward and feedback system to achieve temporal and spatial regulation of cell wall construction, Forward system is composed of a cell cycle dependent morphological pathway that is regulated by small GTPase, Rholp. In addition, the feedback regulatory system, called cell wall integrity checkpoint was recently studied and characterized in our laboratory.
We found that yeast cells stop growing at the specific stage of the cell cycle after perturbation of 1,3-β-glucan synthesis. These cells arrest with post-replicative DNA but quite low level of Clb2p, and without forming bipolar spindles. wacl (wall-checkpoint defective) mutation that abolishes this arrest causes the accumulation of C1b2p and CLB2 mRNA and leads to formation of bipolar spindles despite 1,3-β-glucan-synthesis perturbation. These results indicate the existence of a novel cell cycle checkpoint to coordinate entry into mitosis, and suggest that Waclp is required to achieve this checkpoint function through a transcriptional regulation of CLB2.

Research Products

• [Journal Article] Homologous subunit of yeast 1, 3-beta-glucan synthase is important for spore wall rigidity in Saccharomycescerevisiae. (2007)
  • Author(s): Ishihara, S.
  • Journal Title: Eukaryotic Cell 6 Pages: 143-56
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Homologous subunit of yeast 1,3-betaglucan synthase is important for spore wall rigidity in Saccharomyces cerevisiae (2007)
  • Author(s): Ishihara, S., Hirata, A., Nogami, S., Latge, J.-P., Ohya, Y.
  • Journal Title: Eukaryotic Cell 6 Pages: 143-156
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Homologous subunit of yeast 1,3-beta-glucan synthase is important forspore wall rigidity in Saccharomyces cerevisiae (2007)
  • Author(s): Ishihara, S
  • Journal Title: Eukaryotic Cell 6(2) Pages: 143-156
• [Journal Article] Involvement of small GTPase Rholp in cell size control in yeast. (2007)
  • Author(s): Kikuchi, Y
  • Journal Title: FEMS Yeast Res 7(4): Pages: 569-78
• [Journal Article] Genetic complexity and QTL mapping of yeast morphological traits. (2007)
  • Author(s): Nogami, S.
  • Journal Title: PLOS Genet. 3(2)
• [Journal Article] Multiple Ca2+ regulatory pathways revealed by morphological phenotyping of calcium-sensitive mutants. (2007)
  • Author(s): Ohnuki, S.,
  • Journal Title: Eukaryotic Cell 6(5) Pages: 817-30
• [Journal Article] Polo-like kinase Cdc5 controls the local activation of Rhol to promote cytokinesis. (2006)
  • Author(s): Yoshida, S
  • Journal Title: Science 313 Pages: 108-111
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Exploring the Mode-of-Action of Bioactive Compounds by Chemical-Genetic Profiling in Yeast. (2006)
  • Author(s): Parsons, A. B.
  • Journal Title: Cell 126 Pages: 611-25
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Pellman, D. Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis. (2006)
  • Author(s): Yoshida, S., Kono, K., Lowery, D. M., Bartolini, S., Yaffe, M. B., Ohya, Y.
  • Journal Title: Science 313 Pages: 108-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Exploring the Mode-of-Action of Bioactive Compounds by Chemical-Genetic Profiling in Yeast. (2006)
  • Author(s): Parsons, A. B., Lopez, A., Givoni, I. E., Williams, D. E., Gray, C. A., Porter, J., Chua, G., Sopko, R., Brost, R. L., Ho, C.-H., Wang, J., Ketela, T., Brenner, C., Brill, J. A., Fernandez, G. E., Lorenz, T. C., Payne, G. S., Ishihara, S., Ohya, Y., Andrews, B., Hughes, T. R., Frey, B. J., Graham, T. R., Andersen, R. J., Boone, C.
  • Journal Title: Cell 126 Pages: 611-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Polo-like kinase Cdc5 controls the local activation of Rhol to promotecytokinesis. (2006)
  • Author(s): Yoshida, S
  • Journal Title: Science 313 Pages: 108-11
• [Journal Article] Investigation of the mechanism of meiotic DNA cleavage by VMA1-derived endonuclease uncovers a meiotic alteration in chromatin structure around the target site. (2006)
  • Author(s): Fukuda T
  • Journal Title: Eukaryot Cell. 6(5) Pages: 981-90
• [Journal Article] Evaluation of image processing programs for accurate measurement of budding and fission yeast morphology (2006)
  • Author(s): Suzuki, G
  • Journal Title: Curr Genet. 49 Pages: 237-247
• [Journal Article] Molecular dissection of ARPl regions required for nuclearmigration and cell wall integri ty checkpoint functionsin Saccharomyces cerevisiae. (2005)
  • Author(s): Igarashi, R.
  • Journal Title: Cell Struct Funct. 30 Pages: 57-67
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Involvement of actin and polarisome in morphologicalchange during spore germination of Saccharomcescerevisiae. (2005)
  • Author(s): Kono, K.
  • Journal Title: Yeast 30 Pages: 129-139
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] High-dimensional and large-scale phenotyping of yeastmutants. (2005)
  • Author(s): Ohya, Y.
  • Journal Title: Proc Natl Acad Sci USA. 102 Pages: 19015-20
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular dissection of ARP1 regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae. (2005)
  • Author(s): Igarashi, R., Suzuki, M., Nogami, S., Ohya, Y.
  • Journal Title: Cell Struct Funct. 30 Pages: 57-67
  • NAID: 130004137499
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] (2005) Involvement of act in and polarisome in morphological change during spore germination of Saccharomyces cerevisiae. (2005)
  • Author(s): Kono, K., Matsunaga, R., Hirata, A., Suzuki, G., Abe, M., Ohya, Y.
  • Journal Title: Yeast 30 Pages: 129-139
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High-dimensional and large-scale phenotyping of yeast mutants. (2005)
  • Author(s): Ohya, Y., Sese, J., Yukawa, M., Sano, F., Nakatani, Y., Saito, TL., Saka. A., Fukuda, T., Ishihara, S., Oka, S., Suzuki, G., Watanabe, M., Hirata, A., Ohtani, M., Sawai, H., Fraysse, N., Latge, JP., Francois, JM., Aebi, M., Tanaka, S., Muramatsu, S., Araki, H., Sonoike, K., Nogami, S., Morishita, S.
  • Journal Title: Proc Natl Acad Sci USA. 102(52) Pages: 19015-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High-dimensional and large-scale phenotyping of yeast mutants (2005)
  • Author(s): Ohya, Y.
  • Journal Title: Proc.Natl.Acad.Sci.USA 102 Pages: 19015-19020
• [Journal Article] Molecular dissection of ARP1 regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae (2005)
  • Author(s): Igarashi, R
  • Journal Title: Cell Struct Funct 30 Pages: 57-67
• [Journal Article] Recruitment of Rec Ahomologs Dmc1p and Rad51p to the double-strandbreak repair site initiated by meiosis-specific endonuclease VDE (PI-SceI). (2005)
  • Author(s): Fukuda, T.
  • Journal Title: Mol Genet Genomics 275 Pages: 204-214
• [Journal Article] Involvement of actin and polarisomein morphological change during spore germination of Saccharomyces cerevisiae. (2005)
  • Author(s): Kono, K
  • Journal Title: Yeast 30 Pages: 129-139
• [Journal Article] Hoxcode in embryos of Chinese soft-shelled turtle Pelodiscussinensis correlates with the evolutionary innovation in the turtle. (2005)
  • Author(s): Umeda
  • Journal Title: J Biol Chem. 280 Pages: 1613-1924
• [Book] 実験医学増刊号Vol. 23 「出芽酵母の細胞壁合成をモニターする新規チェックポイント機構」(分担執筆) (2007)
  • Author(s): 関谷瑞穂
  • Total Pages: 5
  • Publisher: 羊土社
  • Description: 「研究成果報告書概要(和文)」より