| Project/Area Number |
17370069
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Kyoto University |
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Principal Investigator |
UEMURA Tadashi Kyoto Univ., Graduate School of Biostudies, Professor, 大学院生命科学研究科, 教授 (80213396)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2006: ¥9,200,000 (Direct Cost: ¥9,200,000)
Fiscal Year 2005: ¥5,300,000 (Direct Cost: ¥5,300,000)
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| Keywords | cofilin / actin / epithelium / neuronal process / mouse |
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| Research Abstract |
Cofilin, a key regulator of actin filament dynamics, is inactivated by phosphorylation at Ser-3 by LIM-kinases and is reactivated by dephosphorylation by a family of protein phosphatases, termed Slingshot (SSH). The human or mouse genome has three copies of Ssh genes (Ssh1-Ssh3). Previous in situ hybridization analyses revealed characteristic patterns of expression of each of the mouse ssh genes in both neuronal and non-neuronal tissues. Here we studied levels of endogenous expression of individual gene products and showed that expression of Ssh-2 and that of Ssh-3 are high in neural and epithelial tissues, respectively. To explore roles of Ssh-3 in epithelial tissues, we isolated derivatives of MDCK cells in which Ssh-3 can be knocked down upon addition of doxycycline. Because we detected Ssh-1 in MDCK cells, we further isolated sublines in which both Ssh-1 and Ssh-3 are knocked down and have been studying phenotypes of cell shape. To examine in vivo roles of Ssh-3 in epithelial morphogenesis, we generated Ssh-3-knock out mice. The Ssh-3-knock out mice are viable and fertile in two different backgrounds and we have been hunting for phenotypes of various epithelial tissues, such as mammary glands and kidney.
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