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Medaka mutants of organogenesis : an animal model for human diseases

Research Project

Project/Area Number 17370078
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionTokyo Institute of Technology

Principal Investigator

KUDO Akira  Tokyo Institute of Technology, Biological Information, Professor, 大学院生命理工学研究科, 教授 (70178002)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2006: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2005: ¥7,900,000 (Direct Cost: ¥7,900,000)
Keywordsmedaka / mutant / positional cloning / bone / heart / fin / human diseases / animal model
Research Abstract

We have screened for mutants defective in organogenesis in the medaka, Oryzias latipes. Males were mutagenized with ethylnitrosourea (ENU), and we identified mutants that were defective in the development of blood cells and various organs, including heart, blood vessels, ear, and fins. In addition, larvae were stained with alizarin red, and we isolated bone mutants. Finally we established 53 mutations affecting organogenesis, and some mutants exhibited a unique phenotype that has not been described in zebrafish. After establishing selected 20 mutant clones, we have started the positional cloning and characterization of mutants, and several candidate genes are available. For characterization, GFP transgenic lines specific for bone and blood vessel have been established, and then were mated with mutant clones to analyze their defects. A hypochromic anemia mutant, whiteout (who), that shows an elongated morphology of blood cells and little hemoglobin activity, was caused by a mutation in the gene for □-aminolevulinic acid dehydratase (ALAD). Another mutant, unextended fin (ufi), which shows the defect of fin extension resulting a shrunken and short fin, was caused by a mutation in the gene for hoxb8, displaying non-canonical wnt pathway defects. Medaka mutants possibly represent models for the human diseases.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (38 results)

All 2007 2006 2005 Other

All Journal Article (33 results) Book (5 results)

  • [Journal Article] Dual effects of the membrane-anchored MMP regulator RECK on chondrogenic differentiation of ATDC5 cells.2007

    • Author(s)
      Kondo, S., Shukunami, C., Morioka, Y., Matsumoto, N., Takahashi, R., Oh, J., Atsumi, T., Umezawa, A., Kudo, A., Kitamura, H., Hiraki, Y., Noda
    • Journal Title

      J. Cell Sci. 120

      Pages: 849-857

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cellular and molecular processes of regeneration, with special emphasis on fish fins (review)2007

    • Author(s)
      Nakatani, Y., Kawakami, A., Kudo, A.
    • Journal Title

      Dev. Growth Differ. 49

      Pages: 145-154

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in adult liver and spleen.2007

    • Author(s)
      Moriyama, A., Kii, I., Sunabori, T., Kurihara, S., Takayama, I., Shimazaki, M., Tanabe, H., Oginuma, M., Fukayama, M., Matsuzaki, Y., Saga' Y., Kudo, A.
    • Journal Title

      Genesis 45

      Pages: 90-100

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Multinucleate osteoclasts in medaka as evidence of active bone remodeling.2007

    • Author(s)
      Nemoto, Y., Higuchi, K., Baba, O., Kudo, A^+., Takano, Y.^+ (^+:equal correspondence)
    • Journal Title

      Bone 40

      Pages: 399-408

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dual effects of the membrane-anchored MMP regulator RECK on chondrogenic differentiation of ATDC5 cells.2007

    • Author(s)
      ^*Kondo, S., Shukunami, C., Morioka, Y., Matsumoto, N., Takahashi, R., Oh, J., Atsumi, T., Umezawa, A., Kudo, A., Kitamura, H., Hiraki, Y., Noda
    • Journal Title

      J.Cell Sci. 120

      Pages: 849-857

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cellular and molecular processes of regeneration, with special emphasis on fish fins (review)2007

    • Author(s)
      ^*Nakatani, Y., Kawakami, A., Kudo, A.
    • Journal Title

      Dev.Growth Differ. 49

      Pages: 145-154

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in adult liver and spleen.2007

    • Author(s)
      *Moriyama, A., Kii, I., Sunabori, T., Kurihara, S., Takayama, I.Shimazaki, M., Tanabe, H., Oginuma, M., Fukayama, M., Matsuzaki, Y., Saga' Y., Kudo, A.
    • Journal Title

      Genesis 45

      Pages: 90-100

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Multinucleate osteoclasts in medaka as evidence of active bone remodeling.2007

    • Author(s)
      ^*Nemoto, Y., Higuchi, K., Baba, O., Kudo, A^+., Takano, Y.^+(^+ : equal correspondence)
    • Journal Title

      Bone 40

      Pages: 399-408

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Clinical Calcium[Periostin function in the periodontal ligament and the periosteum]2007

    • Author(s)
      ^*Kii, I., Kudo, A.
    • Journal Title

      Iyaku Ja-narusha. 17-2

      Pages: 62-68

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Y. Formation of acellular cementum-like layers with and without extrinsic fiber insertion along inert bone surfaces of aging c-Src gene knockout mice.2006

    • Author(s)
      Baba, O., Miyata, A., Abe, T., Shibata, S., Nakano, Y., Oda, T., Kudo, A., Takano
    • Journal Title

      Eur. J. Oral Sci. 114

      Pages: 524-534

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Periostin is an extracellular matrix protein required for eruption ofincisors in mice.2006

    • Author(s)
      Kii, I., Amizuka, N., Minqi, L., Kitajima, S., Saga, Y., Kudo, A.
    • Journal Title

      Biochem. Biophys. Res. Com 342

      Pages: 766-772

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Medaka unextented-fin mutants suggest a role for Hoxb8a in cell migration and osteoblast differentiation during appendage formation.2006

    • Author(s)
      Sakaguchi, S., Nakatani, Y., Takamatsu, N., Hori, H., Kawakami, A., Inohaya, K., Kudo, A.
    • Journal Title

      Dev. Biol. 293

      Pages: 426-438

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] The vascular anatomy of the developing medaka, Oryzias latipes : A complementary fish model for the cardiovascular research of vertebrate.2006

    • Author(s)
      Fujita, M., Isogai, S., Kudo, A
    • Journal Title

      Dev Dyn. 235

      Pages: 734-746

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Formation of acellular cementum-like layers with and without extrinsic fiber insertion along inert bone surfaces of aging c-Src gene knockout mice.2006

    • Author(s)
      ^*Baba, 0., Miyata, A., Abe, T., Shibata, S., Nakano, Y., Oda, T., Kudo, A., Takano, Y.
    • Journal Title

      Eur.J.Oral Sci. 114

      Pages: 524-534

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Periostin is an extracellular matrix protein required for eruption ofincisors in mice.2006

    • Author(s)
      ^*Kii, I., Amizuka, N., Minqi, L., Kitajima, S., Saga, Y., Kudo, A.
    • Journal Title

      Biochem.Biophys.Res.Com. 342

      Pages: 766-772

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] unextented-fin mutants suggest a role for Hoxb8a in cell migration and osteoblast differentiation during appendage formation.2006

    • Author(s)
      ^*Sakaguchi, S., Nakatani, Y., Takamatsu, N., Hori, H., Kawakami, A., Inohaya, K., Kudo, A.Medaka
    • Journal Title

      Dev.Biol. 293

      Pages: 426-438

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Vascular anatomy of the developing medaka, Oryzias latipes : A complementary fish model for the cardiovascular research of vertebrate2006

    • Author(s)
      ^*Fujita, M., Isogai, S., Kudo, A.
    • Journal Title

      Dev Dyn. 235

      Pages: 734-746

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Action of the fasciclinl family-Function of periostin in vertebra-2006

    • Author(s)
      ^*Kii, I, Kudo, A.
    • Journal Title

      Seikagaku. 78-1

      Pages: 16-26

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The vascular anatomy of the developing medaka, Oryzias latipes : A complementary fish model for the cardiovascular ressarch of vertebrate.2006

    • Author(s)
      Fujita, M., Isogai, S., Kudo, A.
    • Journal Title

      Dev Dyn. 235

      Pages: 734-746

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Medaka unextented-fin mutants suggest a role for Hoxb8a in cell migration and osteoblast differentiation during appendage formation2006

    • Author(s)
      Sakaguchi, S., Nakatani, Y., Takamatsu, N., Hori, H., Kawakami, A., Inohaya, K., Kudo, A.
    • Journal Title

      Dev.Biol. (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The vascular anatomy of the developing medaka, Oryzias latipes : A complementary fish model for the cardiovascular research of vertebrate.2006

    • Author(s)
      Fujita, M., Isogai, S., Kudo, A.
    • Journal Title

      Dev Dyn. 235

      Pages: 734-746

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Self-assembled RANK induces osteoclastogenesis ligand-independently.2005

    • Author(s)
      Kanazawa, K., Kudo, A.
    • Journal Title

      J. Bone Miner. Res. 20

      Pages: 2053-2060

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Messenger RNA expression of Periostin and Twist transiently decrease by occlusal hypofunction in mouse periodontal ligament.2005

    • Author(s)
      Afanador, E., Yokozeki, M., Oba, Y., Kitase, Y., Takahashi, T., Kudo, A., Moriyama, K
    • Journal Title

      Arc. Oral Biol. 50

      Pages: 1023-1031

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] TRAF2 is essential for TNF□-induced osteoclastogenesis.2005

    • Author(s)
      Kanazawa, K., Kudo, A.
    • Journal Title

      J. Bone Miner. Res. 20

      Pages: 840-847

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Y. Retionic acid-metabolizing enzyme Cyp26a1 is essential for determining territories of hindbrain and spinal cord in zebrafish.2005

    • Author(s)
      Emoto, Y., Wada, H., Okamoto, H., Kudo, A., Imai
    • Journal Title

      Dev. Biol. 278

      Pages: 415-427

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Self-assembled RANK induces osteoclastogenesis ligand-independently.2005

    • Author(s)
      ^*Kanazawa, K., Kudo, A.
    • Journal Title

      J.Bone Miner.Res. 20

      Pages: 2053-2060

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Messenger RNA expression of Periostin and Twist transiently decrease by occlusal hypofunction in mouse periodontal ligament.2005

    • Author(s)
      ^*Afanador, E., Yokozeki, M., Oba, Y., Kitase, Y., Takahashi, T., Kudo, A., Moriyama, K.
    • Journal Title

      Arc.Oral Biol. 50

      Pages: 1023-1031

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] TRAF2 is essential for TNF□-induced osteoclastogenesis.2005

    • Author(s)
      ^*Kanazawa, K., Kudo, A.
    • Journal Title

      J.Bone Miner.Res. 20

      Pages: 840-847

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Retionic acid-metabolizing enzyme Cyp26al is essential for determining territories of hindbrain and spinal cord in zebrafish.2005

    • Author(s)
      ^*Emoto, Y., Wada, H., Okamoto, H., Kudo, A., Imai, Y.
    • Journal Title

      Dev.Biol. 278

      Pages: 415-427

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Self-assembled RANK induces osteoclastogenesis ligand-independently.:2005

    • Author(s)
      Kanazawa, K., Kudo, A.
    • Journal Title

      J.Bone Miner.Res. 20

      Pages: 2053-2060

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Messenger RNA expression of Periostin and Twist transiently decrease by occlusal hypofunction in mouse periodontal ligament.:2005

    • Author(s)
      Afanador, E., Yokozeki, M., Oba, Y., Kitase, Y., Takahashi, T., Kudo, A., Moriyama, K.
    • Journal Title

      Arc.Oral Biol. 50

      Pages: 1023-1103

    • Related Report
      2005 Annual Research Report
  • [Journal Article] TRAF2 is essential for TNFα-induced osteoclastogenesis.:2005

    • Author(s)
      Kanazawa, K., Kudo, A.
    • Journal Title

      J.Bone Miner.Res. 20

      Pages: 840-847

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Periostin is an extracellular matrix protein required for eruption ofincisors in mice

    • Author(s)
      Kii, I., Amizuka, N., Minqi, L., Kitajima, S., Saga, Y., Kudo, A.
    • Journal Title

      Biochem.Biophys.Res.Com. (in press)

    • Related Report
      2005 Annual Research Report
  • [Book] Clinical Calcium 「ペリオスチンと歯根膜・骨外膜」2007

    • Author(s)
      喜井勲, 工藤明
    • Total Pages
      7
    • Publisher
      医薬ジャーナル社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Clinical Calcium「ペリオスチンと歯根膜・骨外膜」2007

    • Author(s)
      喜井勲, 工藤明
    • Publisher
      医薬ジャーナル社
    • Related Report
      2006 Annual Research Report
  • [Book] ファシクリンIファミリータンパク質の働き-脊椎動物に存在するペリオスチンの機能解析-2006

    • Author(s)
      喜井勲, 工藤明
    • Total Pages
      11
    • Publisher
      生化学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] ファミクリンIファミリータンパク質の働き-脊椎動物に存在するペリオスチンの機能解析-2006

    • Author(s)
      喜井勲, 工藤明
    • Publisher
      生化学
    • Related Report
      2006 Annual Research Report
  • [Book] ファシクリンIファミリータンパク質の働き-脊椎動物に存在するペリオスチンの機能解析-2006

    • Author(s)
      喜井勲, 工藤明
    • Publisher
      日本生化学会
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2021-12-17  

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