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分子育種基盤としてのストレス制御因子の解析

Research Project

Project/Area Number 17380002
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionThe University of Tokyo

Principal Investigator

UCHIMIYA Hirofumi  The University of Tokyo, Institute of Molecular and Cellular Biosciences, Professor (50142229)

Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥17,230,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2007: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2006: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 2005: ¥5,500,000 (Direct Cost: ¥5,500,000)
Keywordsstress response / cell death / rice / arabidopsis / co-factor / molecular breeding / シグナル因子 / NDA / ストレス / 酸化還元因子 / 代謝物 / シロイヌナズナ / 活性酸素 / メタボローム / プログラム細胞死 / NAD代謝 / ミトコンドリア
Research Abstract

Plants habituating one place possess the ability to grow in the environment where various constrains are exists. Thus, plants may have homeostasis to adjust environment by regulating ideal organ and tissue development. In this study, stress responses of plants were examined in view of cell death processes. Thus, I used many transgenic plants for the investigation along this line. Namely, stress responses and metabolites regulating oxidative and reductive factors, and the interaction to cell death factors were analyzed. Results obtained in this investigation indicated that DFR overproducers regulated the response to stresses. Analysis of internal metabolites suggested that organic substances were most altered in such plants, whereas amino acids levels and other metabolites ware not changed. In cultured cells, glutathione level was highly up-regulated, which may indicates the basis of stress tolerance. In the case of DFR transgenic line, molecular basis for the stress resistance may be applied to other breeding strategies to improve plant quality.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (43 results)

All 2007 2006 2005 Other

All Journal Article (34 results) (of which Peer Reviewed: 11 results) Presentation (7 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Mutual Regulation of Arabidopsis thaliana Ethylene-responsive Element Binding Protein and a Plant Floral Homeotic Gene, APETALA22007

    • Author(s)
      Ogawa, T., H. Uchimiya, M. Kawai-Yamada
    • Journal Title

      Ann. Bot. 99

      Pages: 239-244

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltran sferase (AtNMNAT) is required for the growth of pollen tub2007

    • Author(s)
      Hashida, S., H. Takahashi, M. Kawai-Yamada, H. Uchimiya
    • Journal Title

      Plant Journal 49

      Pages: 694-703

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cell death suppressor, Arabidopsis BI-1, is associated with calmodulin-binding and ion homeostasis.2007

    • Author(s)
      Ihara-Ohori, Y., M. Nagano, S. Muto, H. Uchimiya, M. Kawai-Yamada
    • Journal Title

      Plant Phys. 143

      Pages: 650-660

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Mutual Regulation of Arabidopsis thaliana Ethylene-responsive Element Binding Protein and a Plant Floral Homeotic Gene, APETALA22007

    • Author(s)
      Ogawa, T., et al.
    • Journal Title

      Ann. Bot. 99

      Pages: 239-244

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase (AtNMNAT) is required for the growth of pollen tub2007

    • Author(s)
      Hashida, S., et al.
    • Journal Title

      Plant Journal 49

      Pages: 694-703

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cell death suppressor, Arabidopsis BI-1, is associated with calmodulin-binding and ion homeostasis.2007

    • Author(s)
      Ihara-Ohori, Y., et al.
    • Journal Title

      Plant Phys. 143

      Pages: 650-660

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase (AtNMNAT) is required for the growth of pollen tub2007

    • Author(s)
      Hashida, S., H. Takahashi, M. Kawai-Yamada, H. Uchimiya
    • Journal Title

      Plant Journal 49

      Pages: 694-703

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell death suppressor, Arabidopsis BI-1, is associated with calmodulin-binding and ion homeostasis2007

    • Author(s)
      Ihara-Ohori, Y., M. Nagano, S. Muto, H. Uchimiya, M. Kawai-Yamada
    • Journal Title

      Plant Phys. 143

      Pages: 650-660

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Arabidopsis D-type cyclin CYCD4 controls cell division in the stomatal lineage of the hypocotyl epidermis2007

    • Author(s)
      Kono, A., C. Umeda-Hara, S. Adachi, N. Nagata, M. Konomi, T. Nakagawa, H. Uchimiya, M. Umeda
    • Journal Title

      Plant Cell 19

      Pages: 1265-1277

    • Related Report
      2007 Annual Research Report
  • [Journal Article] The Bax Inehibitor-1 needs a functional electron transport chain for cell death Suppression2007

    • Author(s)
      Oshima, R., K. Yoshinaga, Y. Ihara-Ohori, R. ZFukuda, A. Ohta, H. Uchimiya, M. Kawai-Yamada
    • Journal Title

      FEBS Letters 581

      Pages: 4627-4632

    • Related Report
      2007 Annual Research Report
  • [Journal Article] The Mitochondrial Fission Regulator DRP3B Does Not Regulate Cell Death in Plants2006

    • Author(s)
      Yoshinaga, K., M. Fujimoto, S. Arimura, N. Tsutsumi, H. Uchimiya, M. Kawai-Yamada
    • Journal Title

      Annals of Botany 97

      Pages: 1145-1149

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase.2006

    • Author(s)
      Takahashi, H., M. Hayashi, F. Goto, S. Sato, T. Soga, T. Nishioka, M. Tomita, M. Kawai-Yamada, H. Uchimiya
    • Journal Title

      Ann. Bot. 98

      Pages: 819-825

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Chloroplast NAD kinase is essential for energy transduction through xanthophyll cycle in photosynthesis.2006

    • Author(s)
      Takahashi, H., A. Watanabe, A. Tanaka, S. Hashida, M. Kawai-Yamada., K. Sonoike, Uchimiya, H.
    • Journal Title

      Plant Cell Phys. 47

      Pages: 1678-1682

    • NAID

      10018809609

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The Mitochondrial Fission Regulator DRP3B Does Not Regulate Cell Death in Plants2006

    • Author(s)
      Yoshinaga, K., et al.
    • Journal Title

      Annals of Botany 97

      Pages: 1145-1149

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Evaluation of metabolic alteration in transgenic rice dihydroflavonol-4-reductase.2006

    • Author(s)
      Takahashi, H., et al.
    • Journal Title

      Ann. Bot. 98(Overexpressing)

      Pages: 819-825

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Chloroplast NAD kinase is essential for energy transduction through xanthophyll cycle in photosynthesis.2006

    • Author(s)
      Takahashi, H., et al.
    • Journal Title

      Plant Cell Phys. 47

      Pages: 1678-1682

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The Mitochondrial Fission Regulator DRP3B Does Not Regulate Cell Death in Plants2006

    • Author(s)
      Yoshinaga, K., M.Fujimoto, S.Arimura, N.Tsutsumi, H.Uchimiya, M.Kawai-Yamada
    • Journal Title

      Annals of Botany 97

      Pages: 1145-1149

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Abolition of tapetum suicide program ruins microsporogenesis.2006

    • Author(s)
      Kawanabe, T., T.Ariizumi, M.Kawai-Yamada, H.Uchimiya, K.Toriyama
    • Journal Title

      Plant Cell Physiology 47

      Pages: 784-787

    • NAID

      10017602383

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase.2006

    • Author(s)
      Takahashi, H., M.Hayashi, F.Goto, S.Sato, T.Soga, T.Nishioka, M.Tomita, M.Kawai-Yamada, H.Uchimiya
    • Journal Title

      Annals of Botany 98

      Pages: 819-825

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Chloroplast NAD kinase is essential for energy transduction through xanthophylls cycle in photosynthesis.2006

    • Author(s)
      Takahashi, H., A.Watanabe, A.Tanaka, S.Hashida, M.Kawai-Yamada, K.Sonoike, Uchimiya, H.
    • Journal Title

      Plant Cell Physiology 47

      Pages: 1687-1682

    • NAID

      10018809609

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A small acidic protein 1(SMAP1) mediates responses of the Arabidopsis root to the synthetic auxin 2,4-dichlorophenoxyacetic acid2006

    • Author(s)
      Rahman, A., A.Nakasone, T.Chhun, C.Ooura, K.K.Biswas, H.Uchimiya, S.Tsurumi, T.I.Baskin, A.Tanaka, Y.Oono
    • Journal Title

      Plant Journal 47

      Pages: 788-801

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Analysis of the Arabidopsis thaliana Cell Growth Defect Factor 2 (Cdf2) Suppressing Yeast Cell Proliferation2006

    • Author(s)
      Kim, K-M., Y-S.Lim, I-W.Sul, A.Hirata, M.Kawai-Yamada, H.Uchimiya
    • Journal Title

      Korean J. Genetics 28

      Pages: 201-206

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice.2005

    • Author(s)
      Hayashi, M., H. Takahashi, K. Tamura, J-R. Huang, L-H. Yu, M. Kawai-Yamada, T. Tezuka, H. Uchimiya
    • Journal Title

      Proc. Nat. Acad. Sci. U.S.A. 102

      Pages: 7020-7025

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Functional Analysis of Arabidopsis Ethylene-Responsive Element Binding Protein Conferring Resistance to Bax and Abiotic Stress-Induced Plant Cell Death2005

    • Author(s)
      Ogawa, Taro. L. Pan, M. Kawai-Yamada, L-H. Yu, S. Yamamura, T. Koyama, S. Kitajima, M. Ohme-Takagi, F. Sato, H. Uchimiya
    • Journal Title

      Plant Physiology 138

      Pages: 1436-1445

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A novel Arabidopsis gene causes Bax-like lethality in Saccharomyces cerevisiae2005

    • Author(s)
      Kawai-Yamada, Maki., Y. Saito, L-H. Jin, T. Ogawa, K-M. Kim, L-H. Yu, Y. Tone, A. Hirata, M. Umeda, H. Uchimiya
    • Journal Title

      J. Biol. Chem. 280(47)

      Pages: 39468-39473

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice.2005

    • Author(s)
      Hayashi, M., et al.
    • Journal Title

      Proc. Nat. Acad. Sci. U.S.A. 102

      Pages: 7020-7025

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Functional Analysis of Arabidopsis Ethylene-Responsive Element Binding Protein Conferring Resistance to Bax and Abiotic Stress-Induced Plant Cell Death2005

    • Author(s)
      Ogawa, Taro., et al.
    • Journal Title

      Plant Physiology 138

      Pages: 1436-1445

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A novel Arabidopsis gene causes Bax-like lethality in Saccharomyces cerevisiae2005

    • Author(s)
      Kawai-Yamada, Maki, et al.
    • Journal Title

      J. Biol. Chem. 289

      Pages: 39468-39473

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice.2005

    • Author(s)
      Hayashi, M. et al.
    • Journal Title

      Proc.Nat.Acad.Sci.U.S.A. 107

      Pages: 7020-7025

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Proteome approach to characterize the methylmalonate-semialdehyde dehydrogenase that is regulated by gibberellin2005

    • Author(s)
      Tanaka, N. et al.
    • Journal Title

      Journal of Proteome Research 4・5

      Pages: 1575-1582

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Functional Analysis of Arabidopsis Ethylene-Responsive Element Binding Protein Conferring Resistance to Bax and Abiotic Stress-Induced Plant Cell Death2005

    • Author(s)
      Ogawa, T. et al.
    • Journal Title

      Plant Physiology 138

      Pages: 1436-1445

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mitochondrial Behaviour in the Early Stages of ROS Stress Leading to Cell Death in Arabidopsis thaliana2005

    • Author(s)
      Yoshinaga, K. et al.
    • Journal Title

      Annals of Botany 96

      Pages: 337-342

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A novel Arabidopsis gene causes Bax-like lethality in Saccharomyces cerevisiae2005

    • Author(s)
      Kawai-Yamada, M. et al.
    • Journal Title

      The Journal of Biological Chemistry 280・47

      Pages: 39468-39473

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mammalian Bax initiates plant cell death through organelle destruction2005

    • Author(s)
      Yoshinaga, K. et al.
    • Journal Title

      Plant Cell Reports 24

      Pages: 408-417

    • Related Report
      2005 Annual Research Report
  • [Presentation] Molecular and metabolic signals in oxidative stress tolerance2007

    • Author(s)
      Uchimiya H., Hayashida S., Takahashi H., Kawai-Yamada M.
    • Organizer
      国際嫌気生物学会
    • Place of Presentation
      Matsushima, Japan
    • Year and Date
      2007-11-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] NAD代謝改変による代謝亢進の機構2007

    • Author(s)
      川合真紀, 高橋秀行, 橋田慎之介, 内宮博文
    • Organizer
      第48回日本植物生理学会 シンポジウム 栄養シグナルと植物機能制御
    • Place of Presentation
      愛媛大学(松山)
    • Related Report
      2007 Annual Research Report
  • [Presentation] シロイヌナズナNAD生合成遺伝子の機能解析2007

    • Author(s)
      橋田慎之介, 伊丹勇人, 高橋秀行, 川合真紀, 内宮博文
    • Organizer
      第48回日本植物生理学会
    • Place of Presentation
      愛媛大学(松山)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Nicotinate/nicotinamide mononucleotide adenyltransferase, a key enzyme in the NAD biosynthetic pathway, is essential for pollen tube growth in Arabidopsis thaliana2006

    • Author(s)
      Shin-nosuke Hashida, Hideyuki Talahashi, Maki Kawai-Yamad a, Hirofiimi Uchimiya
    • Organizer
      第20回国際生化学・分子生物学会議/第11回アジア・オセアニア生化学者・分子生物学者連合会議(the 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress)
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Nicotinate/nicotinamide mononucleotide Biology adenyltransferase, a key enzyme in the NAD biosynthetic pathway, is essential for pollen tube growth in Arabidopsis thaliana2006

    • Author(s)
      Shin-nosuke Hashida, et al.
    • Organizer
      the 20th IUBMB International of Biochemistry and Molecular and 11th FAOBMB Congress)
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イネにおけるトウモロコシHm1様遺伝子(YK1)の機能解析2005

    • Author(s)
      林光紀, 高橋秀行, 内宮博文
    • Organizer
      第46回日本植物生理学会年会
    • Place of Presentation
      新潟コンベンションセンター朱鷺メッセ
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 酸化ストレス耐性とNAD代謝解析2005

    • Author(s)
      渡辺綾子, 高橋秀行, 橋田慎之介, 川合真紀, 園池公毅, 内宮博文
    • Organizer
      第23回日本植物細胞分子生物学会京都大会・シンポジウム
    • Place of Presentation
      京都大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.iam.u-tokyo.ac.jp/plantlab/index.html

    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] AtBI-1を用いた雄性不稔植物の製造方法2005

    • Inventor(s)
      鳥山 欽哉, 川邊 隆大, 内宮 博文, 山田 真紀
    • Industrial Property Rights Holder
      国立大学法人東北大学
    • Industrial Property Number
      2005-174312
    • Filing Date
      2005-06-14
    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

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