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Molecular and biological analyses of a homeotic mutant and the regulation of floral organ formation in asparagus

Research Project

Project/Area Number 17380014
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionHokkaido University

Principal Investigator

MASUDA Kiyoshi  Hokkaido University, Field Sci. Cent. For Northern Biosphere, Professor (60157203)

Co-Investigator(Kenkyū-buntansha) YAMAGISHI Masumi  Hokkaido University, Grad. School of Agric., Associate Professor (40210348)
SHIOTSUKI(KANNO) Akira  Tohoku University, Grad. School of Life Science, Associate Professor (10260449)
HOSHINO Yoichiro  Hokkaido University, Field Sci. Cent. For Northern Biosphere, Res. Assi. Prof. (50301875)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,450,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥750,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2006: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2005: ¥8,300,000 (Direct Cost: ¥8,300,000)
Keywordsasparagus / PCR marker / quantitative PCR / supermale line / MADS-box genes / floral organs / 雄性マーカー / 超雄性 / 性判別 / ホメオティック変異 / MADS-Box 遺伝子 / 雌雄性 / ABCモデル / MADS-Box遺伝子 / in situ ハイブダイゼーション
Research Abstract

The gender of asparagus is known to be determined by a male active factor M. Some lines of asparagus cultivars carry a homozygous genotype for M. These lines are practically called supermale, since all of the progeny express male phenotype, when pollens from these lines have been used for pollinaton. Therefore, the establishment of supermale lines of asparagus has been tried, in order to produce seed populations only with male genotype. To isolate the homozygous genotype on M male asparagus plants with andromonoecous phenotype, which are able to seed by self-fertilization, have been used. However, lines with flowers capable of self-fertilization are extremely infrequent, and the establishment of supermale lines strongly depends on cultivars. Asparagus differentiate flowers initially as hermaphroditic morphology, and then mature into female and male flowers by degeneration of the inappropriate floral organ. The identity of floral organs is regulated under the expression of MADS-box genes. Flowers set on a line of 'Gold Schatz' (GS#2) form carpel-like organs at whorl 3, and the reduced expression of class-B genes was found to relate the homeotic change. In addition, floral organs at whorl 3 of 'GS#2' escape the possible degeneration and continue to develop until maturation. The progeny obtained by mating with wild-type asparagus showed only normal phenotype, suggesting that the mutation must be recessive. In future, the establishment of methodology to produce progeny showing the homeotic change may be required. On the other hand, for readily applicable research, we examined diagnostics of female and male asparagus. As a result, the utilization of DNA markers may be efficient for identifying gender of asparagus plants at the juvenile stage. Furthermore, supermale individuals were distinguishable from male individuals by quantitative PCR using the DNA markers, suggesting that supemale carries homozygous genotype on male-activating factor M.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (38 results)

All 2008 2007 2006 2005

All Journal Article (18 results) (of which Peer Reviewed: 10 results) Presentation (20 results)

  • [Journal Article] Selection of 5-methyltryptophan-resistant asparagus with ethyl methanesulfonate treatment2008

    • Author(s)
      T. Sonoda
    • Journal Title

      Acta Horticulturae 776

      Pages: 333-335

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Potential of interspecific hybrids in Asparagaceae.2008

    • Author(s)
      T. Ito
    • Journal Title

      Acta Horticulturae 776

      Pages: 279-284

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Potential of interspecific hybrids in Asparagaceae2008

    • Author(s)
      T. Ito
    • Journal Title

      Acta Horticulturae 776

      Pages: 279-284

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Production and analysis of reciprocal hybrids between Asparagus officinalis L. and A, schoberioides Kunth.2007

    • Author(s)
      T. Ito
    • Journal Title

      Genetic Resources and Crop Evolution 54

      Pages: 1063-1071

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Production and analysis of reciprocal hybrids between Asparagus officinalis L. and A, schoberioides Kunth2007

    • Author(s)
      T. Ito
    • Journal Title

      Genetic Resources and Crop Evolution 54

      Pages: 1063-1071

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Production and analysis of reciprocal hybrids between Asparagus officinalis L. and A. Schoberioides Kunth.2007

    • Author(s)
      Takuro Ito, et. al.
    • Journal Title

      Genetic Resources and Crop Evolution 54

      Pages: 1063-1071

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A vegetative line of asparagus (Asparagus officinalis) with a homeotic change in flower development is correlated with a functional deficiency in class-B MADS-box genes.2006

    • Author(s)
      Y. Asada
    • Journal Title

      Journal of Horticultural Science and Biotechnology 81

      Pages: 874-882

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The structure and expression of SEPALLATA-like genes in Asparagus species (Asparagaceae).2006

    • Author(s)
      A. Kanno
    • Journal Title

      Sexual Plant Reproduction 19

      Pages: 133-144

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Development of male-specific primers in garden asparagus (Asparagus officinalis L.).2006

    • Author(s)
      H. Nakayama
    • Journal Title

      Breeding Science 56

      Pages: 327-330

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A vegetative line of asparagus (Asparagus officinalis) with a Homeotic change in flower development is correlated with a functional deficiency in class-B MADS-box genes2006

    • Author(s)
      Y. Asada
    • Journal Title

      Journal of Horticultural Science and Biotechnology 81

      Pages: 874-882

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The structure and expression of SEPALLATA-like genes in Asparagus species (Asparagaceae)2006

    • Author(s)
      A. Kanno
    • Journal Title

      Sexual Plant Reproduction 19

      Pages: 133-144

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Development of male-specific primers in garden asparagus (Asparagus officinalis L.)2006

    • Author(s)
      H. Nakayama
    • Journal Title

      Breeding Science 56

      Pages: 327-330

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A vegetative line of asparagus (Asparagus officinalis) with a homeotic change in flower development is correlated with a functional deficiency in class-B MADS-box genes.2006

    • Author(s)
      Y.Asada, et al.
    • Journal Title

      Journal of Horticultural Science and Biotechnology 81

      Pages: 874-882

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genomic organization of the AODEF gene in Asparagus officinalis L.2005

    • Author(s)
      T. Ito
    • Journal Title

      Genes & Genetic Systems 80

      Pages: 95-103

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Molecular phylogeny of the genus Asparagus (Asparagaceae) inferred from plastid petBintron and petD-rpoA intergenic spacer sequences.2005

    • Author(s)
      T. Fukuda
    • Journal Title

      Plant Species Biology 20

      Pages: 121-132

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Molecular identification of Tiandong derived from Asparagus cochinchinensis (Lour.) Merrill by two typical deletions in cpDNA2005

    • Author(s)
      T. Fukuda
    • Journal Title

      Natural Medicines 59

      Pages: 91-94

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Molecular phylogeny of the genus Asparagus (Asparagaceae) inferred from plastid petB intron and petD-rpoA intergenic spacer sequences2005

    • Author(s)
      T. Fukuda
    • Journal Title

      Plant Species Biology 20

      Pages: 121-132

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Construction of a micro-library enriched with genomic replication origins of carrot somatic embryos by laser microdissection2005

    • Author(s)
      N.Murata et al.
    • Journal Title

      Plant Physiology and Biochemistry 43

      Pages: 513-519

    • Related Report
      2005 Annual Research Report
  • [Presentation] アスパラガスホメオティック変異系統‘GS2'の交雑後代における変異回復について2007

    • Author(s)
      安達 由美子
    • Organizer
      園芸学会
    • Place of Presentation
      香川大学(高松市)
    • Year and Date
      2007-09-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] Flower morphogenesis in progenies of 'GS2', a homeotic mutant of asparagus2007

    • Author(s)
      Y. Adachi
    • Organizer
      Horticultural Congress, Heisei 19-nendo Autumn Meeting
    • Place of Presentation
      Kagawa Univ.
    • Year and Date
      2007-09-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アスパラガスホメオティック突然変異'GS2'の交雑後代における変異回復について2007

    • Author(s)
      安達由美子
    • Organizer
      園芸学会平成19年度秋季大会
    • Place of Presentation
      香川大学(高松市)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アスパラガス(Asparagus officinalils)における雌雄判別マーカーの改変2006

    • Author(s)
      中山北斗
    • Organizer
      園芸学会平成18年度春季大会
    • Place of Presentation
      千葉大学(千葉市)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アスパラガス(Asparagus officinalils L)とキジカクシ(A schoberioides)の交配特性2006

    • Author(s)
      林有子
    • Organizer
      園芸学会平成18年度春季大会
    • Place of Presentation
      千葉大学(千葉市)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 定量PCRによる雌雄および超雄性アスパラガス個体の判別2006

    • Author(s)
      安達由美子
    • Organizer
      園芸学会平成18年度秋季大会
    • Place of Presentation
      長崎大学(長崎市)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNAマーカーを用いたアスパラガス雌雄判別法の簡便化と近縁種への応用2006

    • Author(s)
      菅野明
    • Organizer
      園芸学会平成18年度秋季大会
    • Place of Presentation
      長崎大学(長崎市)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Flower development in a vegetative line of asparagus (Asparagus officinalis L.) that represents a putative homeotic mutation2006

    • Author(s)
      K. Masuda
    • Organizer
      27th International Horticultural Congress & Exhibition
    • Place of Presentation
      Seoul, Korea (COEX Convention & Exhibition Center)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of male-specific primers in garden asparagus (Asparagus officinalis L.)2006

    • Author(s)
      H. Nakayama
    • Organizer
      Horticultural Congress, Heisei 18-nendo Spring Meeting
    • Place of Presentation
      Chiba Univ.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Crossing characteristic of Asparagus officinalis L. and A. schoberioides2006

    • Author(s)
      Y. Hayashi
    • Organizer
      Horticultural Congress, Heisei 18-nendo Spring Meeting
    • Place of Presentation
      Chiba Univ.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of female, male and super-male asparagus plants by quantitative real-time PCR method2006

    • Author(s)
      Y. Adachi
    • Organizer
      Horticultural Congress, Heisei 18-nendo Autumn Meeting
    • Place of Presentation
      Nagasaki Univ.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Simple method for identification of male and female asparagus and its application to other Asparagus species2006

    • Author(s)
      A. Kanno
    • Organizer
      Horticultural Congress, Heisei 18-nendo Autumn Meeting
    • Place of Presentation
      Nagasaki Univ.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] エチルメタンスルホン酸処理によるアスパラガスの5-メチルトリプトファン耐性株の選抜2005

    • Author(s)
      林有子
    • Organizer
      園芸学会平成17年度春季大会
    • Place of Presentation
      筑波大学(つくば市)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 食用アスパラガスと日本在来種ハマタマボウキ(Asparagus kiusianus)の種間雑種作出2005

    • Author(s)
      伊藤卓朗
    • Organizer
      園芸学会平成17年度秋季大会
    • Place of Presentation
      東北大学(仙台市)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Potential of interspecific hybrids in the genus Asparagus2005

    • Author(s)
      T. Ito
    • Organizer
      XIth International Asparagus Symposum
    • Place of Presentation
      Horst, Netherland (Hotel Asteria)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Selection of 5-methyltryptophan-resistant asparagus with ethyl methanesulfonate treatment2005

    • Author(s)
      T. Sonoda
    • Organizer
      XIth International Asparagus Symposum
    • Place of Presentation
      Horst, Netherland (Hotel Asteria)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Selection of 5-methyltryptophan-resistant asparagus treated with ethyl methanesulphonate2005

    • Author(s)
      Y. Hayashi
    • Organizer
      Horticultural Congress, Heisei 17-nendo Spring Meeting
    • Place of Presentation
      Tsukuba Univ.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Interspecific hybrid between garden asparagus and Asparagus kiusianus, whichi is domestic species in Japan2005

    • Author(s)
      T. Ito
    • Organizer
      Horticultural Congress, Heisei 17-nendo Autumn meeting
    • Place of Presentation
      Tohoku Univ.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Potential of interspecific hybrids in the genus Asparagus2005

    • Author(s)
      T. Ito
    • Organizer
      Xith International Asparagus Symposum
    • Place of Presentation
      Horst, Netherlands (Hotel Asteria)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Selection of 5-methyltryptophan-resistant asparagus with ethyl methanesulfonate treatment2005

    • Author(s)
      T. Sonoda
    • Organizer
      Xith International Asparagus Symposum
    • Place of Presentation
      Horst, Netherlands (Hotel Asteria)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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