Exploration of the sequential fundamental processes in the early infection stage of Ralstonra solanacearum

• Project/Area Number: 17380031
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kochi University
• Principal Investigator: OHNISHI Kouhei Kochi University, Science Research Center, Professor (50211800)
• Co-Investigator(Kenkyū-buntansha): HIKICHI Yasufumi Kochi University, Faculty of Agriculture, Professor (70291507) ; KIBA Akinori Kochi University, Faculty of Agriculture, Associate professor (50343314)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥16,940,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2006: ¥3,800,000 (Direct Cost: ¥3,800,000); Fiscal Year 2005: ¥8,200,000 (Direct Cost: ¥8,200,000)
• Keywords: gene / plant pathogen / signal transduction / tomato / Solanaceae

Research Abstract

Ralstonia solanacearum first attaches to host plant root surfaces and invades the intercellular spaces of the root cortex. R. solanacearum rapidly multiplies and colonizes within intercellular spaces and vascular parenchyma to finally break into the protoxylem vessels. Plant cell signals are recognized by PrhA on the bacterial outer membrane and transferred to hrpB expression. By using the co-cultivation method of R. solanacearum with Arabidopsis thaliana in the liquid medium, we have investigated the regulation of pathogenicity gene expression, including hrp genes, at these early stages of the infection process. The eps genes responsible for EPS production are known to be induced at the late stage of infection in a cell density-dependent manner by PhcA. While the expression of prhA was constitutive, other genes involved in plant signal cascade and genes in hrp regulon were expressed depending on cell densities. Cell densities of maximal expression for these genes were less than those for eps genes, indicating expression of these genes are repressed after they reach to the maximum. By transposon mutagenesis, PhcA was demonstrated to be a negative regulator of genes in hrp regulon. The expression of phcA itself is known to be activated in a cell density-dependent manner through quorum sensing. Taken all together, we propose the global regulation scheme at early stages of infection involving HrpB and PhcA. After R. solanacearum invades the intercellular spaces, hrpB is induced in response to plant signal and activates hrp regulon, which constructs type III secretion system. R. solanacearum cells can develop with the aid of secreted proteins through the type III system, and cell densities reach to the threshold, in which phcA starts to express. The expressed PhcA represses genes involved in plant signal cascade and hrp regulon, and at the same time activates eps genes and other genes for degradation enzymes.

Research Products

• [Journal Article] Induction of a small heat shock protein and its functional roles in Nicotiana plants in the defense response against Ralstonia solanacearum (2007)
  • Author(s): Maimbo M. Ohnishi K., Hikichi Y., Yoshioka H., Kiba A.
  • Journal Title: Plant Physiol 145 Pages: 1588-1599
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Isolation and expression analysis of candidate genes related to Ralstonia solanacearum-tobacco interaction (2007)
  • Author(s): Kiba A., Maimbo M., Kanda A., Tomiyama H., Ohnishi K., Hikichi Y.
  • Journal Title: Plant Biotechnol. 24 Pages: 409-416
  • NAID: 10021911916
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Global regulation of pathogenicity mechanism of Ralstonia solanacearum (2007)
  • Author(s): Hikichi Y., Yoshimochi T., Tsujimoto S., Shinohara R., Nakaho K., Kanda A., Kiba A., Ohnishi K.
  • Journal Title: Plant Biotechnol. 24 Pages: 149-154
  • NAID: 10021910740
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Amino acid substitutions in GyrA of Burkholderia glumae are implicated in not only oxolinic acid resistance but also fitness on rice plants (2007)
  • Author(s): Maeda Y, Kiba A, Ohnishi K, Hikichi Y.
  • Journal Title: Appl. Environ. Microbiol. 73(4) Pages: 1114-1119
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Induction of a small heat shock protein and its functional roles in Nicotiana plants in the defense response against Ralstonia solanacearum (2007)
  • Author(s): Maimbo, M. Olmishi, K., Hikichi, Y., Yoshioka, H., Kiba, A
  • Journal Title: Plant Physiol 145 Pages: 1588-1599
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isolation and expression analysis of candidate genes related to Ralstonia solanacearum-tobacco interaction (2007)
  • Author(s): Kiba, A., Maimbo, M., Kanda, A., Tomiyama, H., Ohnishi K., Hikichi, Y
  • Journal Title: Plant Biotechnol 24 Pages: 409-416
  • NAID: 10021911916
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Global regulation of pathogenicity mechanism of Ralstonia solanacearum. (2007)
  • Author(s): Hikichi, Y., Yoshimochi, T., Tsujimoto, S., Shinohara, R., Nakaho, K., Kanda A., Kiba, A., Ohnishi, K
  • Journal Title: Plant Bioteclnol 24 Pages: 149-154
  • NAID: 10021910740
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Amino acid substitutions in GyrA of Burkholderia glunme are implicated in not only oxolinic acid resistance but also fitness on rice plants (2007)
  • Author(s): Maeda, Y., Kiba, A., Olmishi., K, Hikichi, Y
  • Journal Title: Appl. Environ. Microbiol 73 Pages: 1114-1119
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Induction of a small heat shock protein and its functional roles in Nicotiana plants in the defense response against Ralstonia solanacearum (2007)
  • Author(s): Maimbo M.Ohnishi K., Hikichi Y., Yoshioka H., Kiba A.
  • Journal Title: Plant Physiol 145 Pages: 1588-1599
  • Peer Reviewed
• [Journal Article] Isolation and expression analysis of candidate genes related to Ralstonia solanacearum-tobacco interaction (2007)
  • Author(s): Kiba A., Maimbo M., Kanda A., Tomiyama H., Ohnishi K., Hikichi Y.
  • Journal Title: Plant Biotechnol 24 Pages: 409-416
  • NAID: 10021911916
  • Peer Reviewed
• [Journal Article] Global regulation of pathogenicity mechanism of Ralstonia solanacearum (2007)
  • Author(s): Hikichi Y., Yoshimochi T., Tsujimoto S., Shinohara R., Nakaho K., Kanda A., Kiba A., Ohnishi K.
  • Journal Title: Plant Biotechnol 24 Pages: 149-154
  • NAID: 10021910740
  • Peer Reviewed
• [Journal Article] Amino acid substitutions in GyrA of Burkholderia glumae are implicated in not only oxolinic acid resistance but also fitness on rice plants. (2007)
  • Author(s): Maeda Y, Kiba A, Ohnishi K, Hikichi Y
  • Journal Title: Appl Environ Microbiol 73(4) Pages: 1114-1119
• [Journal Article] Induction of apoptotic cell death leads to the development of bacterial rot caused by Pseudomonas cichorii (2006)
  • Author(s): Kiba A., Sangawa Y., Ohinishi K., Yao N., Park P., Nakayashiki H., Tosa Y., Mayama S., Hikichi, Y.
  • Journal Title: Mol. Plant-Microbe Interact. 19(2) Pages: 112-122
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Phylogenetic study and multiplex PCR-based detection of Burkholderia plantarii,Burkholderia glumae and Burkholderia gladioli using gyrB and rpoD sequences. (2006)
  • Author(s): Maeda Y, Shinohara H, Kiba A, Ohnishi K, Furuya N, Kawamura Y, Ezaki T, Vandamme P, Tsushima S, Hikichi Y.
  • Journal Title: Int. J. Syst. Evol. Microbiol. 56(5) Pages: 1031-1038
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Comparative analysis of induction pattern of programmed cell death and defense-related responses during hypersensitive cell death and development of bacterial necrotic leaf spots in eggplant (2006)
  • Author(s): Kiba A, Takata O, Ohnishi K, Hikichi Y.
  • Journal Title: Planta 224(5) Pages: 981-994
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Directed evolution of extradiol dioxygenase by a novel in vivo DNA shuffling (2006)
  • Author(s): Xu S., Ju J. Misono H. Ohnishi K.
  • Journal Title: Gene 368 Pages: 126-137
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Induction of apoptotic cell death leads to the development of bacterial rot caused by Pseudomonas cichorii (2006)
  • Author(s): Kiba, A., Sangawa, Y., Olmishi, K., Yao N., Park, E., Nakayashiki, H., Tosa, Y., Mayama, S., Hikichi, Y
  • Journal Title: Mol. Plant-Microbe Interact 19 Pages: 112-122
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phylogenetic study and multiplex PCR-based detection of Burkholderia plantarii. Burkholderia glumae and Burkholderia gladioli using gyrB and rpoD sequences (2006)
  • Author(s): Maeda, Y., Shinohara, H., Kiba, A., Ohnishi, K., Furuya, N. Kawamura, Y., Ezaki, T., Vandanune, P., Tsushima, S., Hikichi, Y
  • Journal Title: Int. J. Syst. Evol. Microbiol 56 Pages: 1031-1038
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparative analysis of induction pattern of programmed cell death and defense-related responses during hypersensitive cell death and development of bacterial necrotic leaf spots in eggplant (2006)
  • Author(s): Kiba, A., Takata, 0., Olmishi, K., Hikichi, Y
  • Journal Title: Planta 224 Pages: 981-994
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Directed evolution of extradiol dioxygenase by a novel in vivo DNA shuffling (2006)
  • Author(s): Xu, S., Ju, J., Misono, H., Ohnishi, K
  • Journal Title: Gene 368 Pages: 126-137
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Induction of apoptotic cell death leads to the development of bacterial rot caused by Pseudomonas cichorii. (2006)
  • Author(s): Kiba A., Sangawa Y., Ohnishi K., Yao N., Park P., Nakayashiki H., Tosa Y., Mayama S., Hikichi Y.
  • Journal Title: Mol. Plant-Microbe Interact 19(2) Pages: 112-122
• [Journal Article] Phylogenetic study and multiplex PCR-based detection of Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli using gyrB and rpoD sequences. (2006)
  • Author(s): Maeda Y, Shinohara H, Kiba A, Ohnishi K, Furuya N, Kawamura Y, Ezaki T, Vandamme P, Tsushima S, Hikichi Y
  • Journal Title: Int J Syst Evol Microbiol 56(5) Pages: 1031-1038
• [Journal Article] Comparative analysis of induction pattern of programmed cell death and defense-related responses during hypersensitive cell death and development of bacterial necrotic leaf spots in eggplant. (2006)
  • Author(s): Kiba A, Takata O, Ohnishi K, Hikichi Y.
  • Journal Title: Planta 224(5) Pages: 981-994
• [Journal Article] Induction of apoptotic cell death leads to the development of bacterial rot caused by Pseudomonas cichorii. (2006)
  • Author(s): Kiba A., Sangawa Y., Ohnishi K., Yao N., Park P., Nakayashiki H., Tosa Y., Mayama S., Hikichi, Y.
  • Journal Title: Mol.Plant-Microbe Interact. 19(2) Pages: 112-122
• [Journal Article] Directed evolution of extradiol dioxygenase by a novel in vivo DNA shuffling. (2006)
  • Author(s): Xu S., Ju J., Misono H., Ohnishi K.
  • Journal Title: Gene (印刷中)
• [Journal Article] N-terminal signal region of FliK is dispensable for length control of the flagellar hook (2005)
  • Author(s): Hirano T., Shibata S., Ohnishi K., Tani T., Aizawa, S. I.
  • Journal Title: Mol. Microbiol. 56(2) Pages: 346-360
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Contribution of folate biosynthesis to Ralstonia solanacearum proliferation in intercellular spaces (2005)
  • Author(s): Shinohara R., Kanda A., Ohnishi K., Kiba A., Hikichi Y.
  • Journal Title: Appl. Environ. Microbiol. 71(1) Pages: 417-22
  • NAID: 120001129726
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Catalog of Micro-Tom tomato responses to common fungal,bacterial,and viral pathogens (2005)
  • Author(s): Takahashi H., Shimizu A., Arie. T., Rosmalawati S., Fukushima S., Kikuchi M., Hikichi Y., Kanda A., Takahashi A., Kiba a., Ohnishi K., Ichinose Y., Taguchi F., Yasuda C., Kodama M., Egusa M., Masuta C., Sawada H., Shibata D., Watanabe Y.
  • Journal Title: J. Gen. Plant. Pathol. 71(1) Pages: 8-22
  • NAID: 10014471856
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] N-terminal signal region of FIiK is dispensable for length control of the flagellar hook (2005)
  • Author(s): Hirano, T., Shibata, S., Olmishi, K., Tani, T., Aizawa, S.I
  • Journal Title: Mol. Microbiol 56 Pages: 346-360
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Contribution of folate biosynthesis to Ralstonia solanacearum proliferation in intercellular spaces (2005)
  • Author(s): Shinohara, R., Kanda, A., Ohnishi, K., Kiba, A., Hikichi, Y
  • Journal Title: Appl. Environ. Microbiol 71 Pages: 417-422
  • NAID: 120001129726
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Catalog of Micro-Torn tomato responses to common fungal, bacterial, and viral pathogens (2005)
  • Author(s): Takahashi, H., Shimizu, A., Arie, T., Rosmalawati, S., Fukushima S., Kikuchi, M., Hikichi, Y., Kanda, A., Takahashi, A., Kiba, A., Ohnishi, K., Ichinose Y., Taguchi, F., Yasuda, C., Kodama, M., Egusa, M., Masuta, C., Sawada, H., Shibata, D., Watanabe, Y
  • Journal Title: J. Gen. Plant. Pathol 71 Pages: 8-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Catalog of Micro-Tom tomato responses to common fungal, bacterial, and viral pathogens. (2005)
  • Author(s): Takahashi H., Shimizu A., Arie T., Rosmalawati S., Fukushima S., Kikuchi M., Hikichi Y., Kanda A., Takahashi A., Kiba a., Ohnishi K., Ichinose Y., Taguchi F., Yasuda C., Kodama M., Egusa M., Masuta C., Sawada H., Shibata D., Watanabe Y.
  • Journal Title: Gen.Plant.Pathol. 71(1) Pages: 8-22
  • NAID: 10014471856
• [Journal Article] Contribution of folate biosynthesis to Ralstonia solanacearum proliferation in intercellular spaces. (2005)
  • Author(s): Shinohara R., Kanda A., Ohnishi K., Kiba A., Hikichi Y.
  • Journal Title: Appl.Environ.Microbiol. 71(1) Pages: 417-422
  • NAID: 120001129726
• [Journal Article] N-terminal signal region of FliK is dispensable for length control of the flagellar hook. (2005)
  • Author(s): Hirano T., Shibata S., Ohnishi K., Tani T., Aizawa, S.I.
  • Journal Title: Mol.Microbiol. 56(2) Pages: 346-360
• [Presentation] Ralstonia solanacearumの感染初期過程における病原性遺伝子発現解析 (2008)
  • Author(s): 大西 浩平、吉用 武史、木場 章範、曵地 康史
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都府
  • Year and Date: 2008-03-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Ralstonia solanacearumのhrpシグナルカスケード発現調節機構 (2008)
  • Author(s): 吉用 武史、木場 章範、大西 浩平、曵地 康史
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都府
  • Year and Date: 2008-03-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pseudomonas cichoriiのpathogenicity islandと病原性の分化 (2008)
  • Author(s): 小柳 諒、田中 将之、大西 浩平、木場 章範、曵地 康史
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都府
  • Year and Date: 2008-03-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 低分子熱ショックタンパク質は植物の細菌感染に対する生体防御に関与する (2008)
  • Author(s): 木場 章範、大西 浩平、吉岡 博文、曵地 康史、
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都府
  • Year and Date: 2008-03-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 低分子熱ショックタンパク質は青枯病菌感染に対する生体防御に関与する (2008)
  • Author(s): Maimbo, M.、大西 浩平、吉岡 博文、曵地 康史、木場 章範
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌市
  • Year and Date: 2008-03-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 青枯病菌感染に対する防御応答におけるアスパラギンリッチタンパク質の関与 (2008)
  • Author(s): 小森 大輔、大西 浩平、曵地 康史、吉岡 博文、木場 章範
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌市
  • Year and Date: 2008-03-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Burkholderia glumae in vitro変異株のoxolinic acid耐性機構とイネにおける生存適応能 (2007)
  • Author(s): 曵地 康史、前田 由紀子、大西 浩平。木場 章範
  • Organizer: 植物微生物研究会第17回研究交流会
  • Place of Presentation: 鹿児島市
  • Year and Date: 2007-09-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pseudomonas cichoriiのhrp遺伝子群はナスに対する病原性に必須であるが、レタスに対する病原性には必須でない (2007)
  • Author(s): 小柳 諒、田中 将之、大西 浩平、木場 章範、曵地 康史
  • Organizer: 植物微生物研究会第17回研究交流会
  • Place of Presentation: 鹿児島市
  • Year and Date: 2007-09-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 青枯病菌のhrp regulonの培養条件依存的発現調節機構の解明 (2007)
  • Author(s): 吉用 武史、木場 章範、大西 浩平、曵地 康史
  • Organizer: 日本遺伝学会第79回大会
  • Place of Presentation: 岡山市
  • Year and Date: 2007-09-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Silencing of small heat shock protein results in suppression of defense-related responses to bacterial wilt pathogen Ralstonia solanacearum (2007)
  • Author(s): Maimbo, M., Ohnishi, K., Hikichi, Y., Yoshioka, H., Kiba, A.
  • Organizer: 平成19年度日本植物病理学会
  • Place of Presentation: 栃木県
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Ralstonia solanacearumにおけるPhcAによるprhIRの発現抑制 (2007)
  • Author(s): 吉用 武史、木場 章範、大西 浩平、曵地 康史
  • Organizer: 平成19年度日本植物病理学会
  • Place of Presentation: 栃木県
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Inventory analysis of genes affecting the hrp regulon expression in Ralstonia solanacearum (2007)
  • Author(s): Zhang, Y., Yoshimochi, T., Kiba, A., Hikichi, Y., Ohnishi, K.
  • Organizer: 平成19年度日本植物病理学会
  • Place of Presentation: 栃木県
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 青枯病菌のhrp遺伝子群の株間における比較解析 (2007)
  • Author(s): Liu Yingqin、曵地 康史、木場 章範、大西 浩平
  • Organizer: 平成19年度日本植物病理学会
  • Place of Presentation: 栃木県
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pseudomonas cichoriiによるレタス腐敗病の発病過程におけるMAPKカスケードの関与 (2007)
  • Author(s): 川上 拓、木場 章範、大西 浩平、曵地 康史
  • Organizer: 平成19年度日本植物病理学会
  • Place of Presentation: 栃木県
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pseudomonas cichoriiSPC9018株のhrpクラスターと新たな病原性遺伝子 (2007)
  • Author(s): 田中 将之、小柳 諒、梶原 茂、北條 広、大西 浩平、木場 章範、曵地 康史
  • Organizer: 平成19年度日本植物病理学会
  • Place of Presentation: 栃木県
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 植物病原菌の病原性制御機構の解明-Ralstonia solanacearumを中心に- (2007)
  • Author(s): 曵地 康史、吉用 武史、木場 章範、大西 浩平
  • Organizer: 第80回日本細菌学会総会
  • Place of Presentation: 大阪府
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Ralstonia solanacearumのhrp遺伝子群の発現制御 (2007)
  • Author(s): 吉用 武史、木場 章範、大西 浩平、曵地 康史
  • Organizer: 第80回日本細菌学会総会
  • Place of Presentation: 大阪府
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 植物細菌Burkholderia glumaeのoxolinic acid耐性機構と耐性株の生存適応能の解析 (2007)
  • Author(s): 前田 由紀子、木場 章範、大西 浩平、曵地 康史
  • Organizer: 第80回日本細菌学会総会
  • Place of Presentation: 大阪府
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] レタス腐敗病の発病過程におけるアポトーシス様プログラム細胞死の関与 (2007)
  • Author(s): 木場 章範、大西 浩平、曵地 康史
  • Organizer: 第80回日本細菌学会総会
  • Place of Presentation: 大阪府
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 青枯病菌の持つLysRタイプ転写調節因子PhcAの機能解析 (2006)
  • Author(s): 吉用 武史、大西 浩平、木場 章範、曵地 康史
  • Organizer: 日本遺伝学会第78回大会
  • Place of Presentation: 茨城県
  • Year and Date: 2006-09-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Global regulation of pathogenicity genes at early stages of the infection process of Ralstonia solanacearum. (2006)
  • Author(s): K. Ohnishi, T. Yoshimochi, M. Adachi, A. Kiba and Y. Hikichi
  • Organizer: The 4th International Bacterial Wilt Symposium
  • Place of Presentation: York, UK
  • Year and Date: 2006-07-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The type II secretion system contributes to invasion of Ralstonia solanacearum into xylem vessels, leading to systemic infection of the bacteria. (2006)
  • Author(s): Y. Hikichi, S. Tsujimoto, K. Nakaho, K. Ohnishi and A. Kiba
  • Organizer: The 4th International Bacterial Wilt Symposium
  • Place of Presentation: York, UK
  • Year and Date: 2006-07-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Global regulation of pathogenicity genes at early stages of the infection process of Ralstonia solanacearum (2006)
  • Author(s): K. Olmishi, T. Yoshimochi, M. Adachi, A. Kiba, Y. Hikichi
  • Organizer: The 4th International Bacterial Wilt Symposium
  • Place of Presentation: York, UK
  • Year and Date: 2006-07-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The type II secretion system contributes to invasion of Ralston/a solanaceanan into xylem vessels, leading to systemic infection of the bacteria (2006)
  • Author(s): Y. Hikichi, S. Tsujimoto, K. Nakaho, K. Ohnishi, A. Kiba
  • Organizer: The 4th International Bacterial Wilt Symposium
  • Place of Presentation: York, UK
  • Year and Date: 2006-07-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Induction of programmed cell death by common and different mechanisms during hypersensitive cell death and development of bacterial necrotic leaf spots in eggplant. (2006)
  • Author(s): A Kiba, O Takata, K Ohnishi, Y Hikichi
  • Organizer: 11th International Conference on Plant Pathogenic Bacteria
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2006-07-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Interaction of type II and type III secretion systems in Ralstonia solanacearum. (2006)
  • Author(s): K Ohnishi, M Adachi, S Tsujimoto, T Yoshimochi, A Kiba, Y Hikichi
  • Organizer: 11th International Conference on Plant Pathogenic Bacteria
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2006-07-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Oxolinic acid-resistance mechanism of Burkholderia glumae and survival fitness of oxolinic acid-resistant bacteria in rice plants. (2006)
  • Author(s): Y Hikichi, Y Maeda, K Ohnishi, Akiba
  • Organizer: 11th International Conference on Plant Pathogenic Bacteria
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2006-07-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Induction of programmed cell death by common and different mechanisms during hypersensitive cell death and development of bacterial necrotic leaf spots in eggplant (2006)
  • Author(s): A. Kiba, O. Takata, K. Olmishi, Y. Hikichi
  • Organizer: llth International Conference on Plant Pathogenic Bacteria
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2006-07-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Interaction of type II and type III secretion systems it Ralstonia solanacearum (2006)
  • Author(s): K. Olutislu, M. Adachi, S. Tsujimoto, T. Yoshimochi, A. Kiba, Y. Hikichi
  • Organizer: llth International Conference on Plant Pathogenic Bacteria
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2006-07-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Oxolinic acid-resistance mechanism of Burkholderia glumoe and survival fitness of oxolinic acid-resistant bacteria in rice plants (2006)
  • Author(s): Y. Hikichi, Y. Maeda, K. Olmishi, A. Kiba
  • Organizer: llth International Conference on Plant Pathogenic Bacteria
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2006-07-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Ralstonia solanacearumのhrp遺伝子群の発現制御 (2005)
  • Author(s): 吉用 武史、木場 章範、大西 浩平、曵地 康史
  • Organizer: 平成17年度日本微生物生態学会
  • Place of Presentation: 福岡市
  • Year and Date: 2005-10-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 植物病原菌Ralstonia solanacearumのhrpレギュロンは植物シグナル伝達系によって調節されている (2005)
  • Author(s): 吉用 武史、足立 雅典、木場 章範、曵地 康史、大西 浩平
  • Organizer: 日本遺伝学会第77回大会
  • Place of Presentation: 東京都
  • Year and Date: 2005-09-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 植物病原糸状菌・細菌およびウイルス感染に対するナス科モデル植物"マイクロトム"トマトの応答 (2005)
  • Author(s): 渡辺 雄一郎・堀孝 一・有江 力・Syofi Rosmalawati・福島 すみれ・菊池 麻里・曵地 康史・神田 絢美・高橋 彰子・木場 章範・大西 浩平・一瀬 勇規・田口 富美子・安田 千裕・児玉 基一郎・江草 真由美・増田 税・澤田 宏之・柴田 大輔・高橋 英樹・清水 綾乃
  • Organizer: 平成17年度日本植物病理学会
  • Place of Presentation: 静岡市
  • Year and Date: 2005-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] gyrBとrpoDの塩基配列を基にしたBurkholderia glumae, B.plantariiおよびB.gladioliの系統解析 (2005)
  • Author(s): 前田 由紀子、篠原 弘亮、大西 浩平、木場 章範、古谷 成入、封馬 誠也、曵地 康史
  • Organizer: 平成17年度日本植物病理学会
  • Place of Presentation: 静岡市
  • Year and Date: 2005-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pseudomonas cichoriiのhrp遺伝子群に挿入されたDNA領域の病原性への関与 (2005)
  • Author(s): 梶原 茂、北條 広、木場 章範、大西 浩平、曵地 康史
  • Organizer: 平成17年度日本植物病理学会
  • Place of Presentation: 静岡市
  • Year and Date: 2005-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Ralstonia solanacearumのhrpレギュロンの発現はPhcAによって制御されている (2005)
  • Author(s): 吉用 武史、木場 章範、大西 浩平、曵地 康史
  • Organizer: 平成17年度日本植物病理学会
  • Place of Presentation: 静岡市
  • Year and Date: 2005-03-29
  • Description: 「研究成果報告書概要(和文)」より