The regulation of transcription factors by an insect steroid hormone and its role on molting and metamorphosis

• Project/Area Number: 17380033
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Hirosaki University
• Principal Investigator: HIRUMA Kiyoshi Hirosaki University, 農学生命科学部, 教授 (70374816)
• Co-Investigator(Kenkyū-buntansha): TOMITA Shuichiro , 農業生物資源研究所, 主任研究員 (30360457)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,560,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥1,560,000); Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2006: ¥4,500,000 (Direct Cost: ¥4,500,000); Fiscal Year 2005: ¥5,300,000 (Direct Cost: ¥5,300,000)
• Keywords: 遺伝子 / 応用動物 / 昆虫 / 発生・分化 / 昆虫生理学 / ホルモンレセプター / 幼若ホルモン / エクダイソン / カイコ / 器官培養 / 転写因子 / コミットメント / タバコスズメガ / 細胞培養 / ドーパデカルボキシラーゼ

Research Abstract

(1) 昆虫皮膚のメラニン化、硬化現象に重要な酵素、ドーパデカルボキシラーゼ(DDC)発現の分子メカニズムを解明した。DDCはエクダイソンにより制御されているが、エクダイソンレセプターからその発現までのカスケードを明らかにした。またその中の1つの転写因子HR4がエクダイソンにより制御されているばかりでなく、その合成に関与していることを見出した。この系は哺乳類には存在せず、昆虫に特異的な新規薬剤開発のヒントになると思われる。(2) 蛹発育時に不要なcrochet細胞の細胞死がホルモンばかりでなく、栄養が重要な役割を持っていることを明らかにした。(3) 幼若ホルモン(JH)が成虫原基の成長を積極的に抑制するという新規作用を見出し、そのうえインスリンシグナル経路以外の栄養を必要としない経路が存在し、細胞の発育を支配していることを見出した。

Research Products

• [Journal Article] The molecular mechanisms of cuticular melanization (2009)
  • Author(s): Hiruma, K., and Riddiford, L.M
  • Journal Title: the ecdysone cascade leading to dopa decarboxylase expression in Manduca sexta. Insect Biochem. Mol. Biol 39 Pages: 245-253
  • Peer Reviewed
• [Journal Article] The molecular mechanisms of cuticular melanization : The ecdysone cascade leading to dopa decarboxylase expression in Manduca sexta (2009)
  • Author(s): Hiruma, K., Riddiford, L. M.
  • Journal Title: Insect Biochemistry and Molecular Biology 39 Pages: 245-253
  • Peer Reviewed
• [Journal Article] 昆虫皮膚のメラニン化の分子機構 (2008)
  • Author(s): 比留間潔
  • Journal Title: 化学と生物 46 Pages: 571-578
  • NAID: 10024334319
• [Journal Article] The role of 20-hydroxyecdysone and juvenile hormone in pupal commitment of the epidermis of the silkworm (2008)
  • Author(s): Muramatsu, D., Kinjoh, T., Shinoda, T. and Hiruma, K.
  • Journal Title: Bombyx mori. Mech. Dev 125 Pages: 411-420
  • Peer Reviewed
• [Journal Article] The coordination of the sequential appearance of MHR4 and dopa decarboxylase during the decline of the ecdysteroid titer at the end of the molt (2007)
  • Author(s): Hiruma, K., and Riddiford, L.M.
  • Journal Title: Mol. Cell. Endocrinol 276 Pages: 71-79
  • Peer Reviewed
• [Journal Article] The coordination of the sequential appearance of MHR4 and dopadecarboxylase during the decline of the ecdysteroid titer at the end of the molt. (2007)
  • Author(s): Hiruma, K. and Riddiford, L. M.
  • Journal Title: Molecular and Cellular Endocrinology 276 Pages: 71-79
  • Peer Reviewed
• [Journal Article] Developmental expression and hormonal regulation of different isoforms of the transcription factor E75 in the tobacco hornworm (2006)
  • Author(s): Keshan, B., Hiruma, K., and Riddiford, L.M.
  • Journal Title: Manduca sexta. Dev. Biol 295 Pages: 623-632
  • Peer Reviewed
• [Journal Article] Juvenile hormone is required to couple imaginal disc formation with nutrition in insects (2006)
  • Author(s): Truman, J. W., Hiruma, K., Alle, J.P. MacWhinnie, S.G.B., Champlin, D.T. and Riddiford, L.M.
  • Journal Title: Science 312 Pages: 1385-1388
  • Peer Reviewed
• [Journal Article] Juvenile hormone is required to couple imaginal disc formation with nutrition in insects (2006)
  • Author(s): Truman, J.W, Hiruma, K., Allee, J.P., MacWhinnie, S.G.B., Champlin, D.T., Riddiford, L.M.
  • Journal Title: Science 312 Pages: 1385-1388
• [Journal Article] Developmental expression and hormonal regulation of different isoforms of the transcription factor E75 in the tobacco hornworm, Manduca sexta (2006)
  • Author(s): Keshan, B., Hiruma, K., Riddiford, L.M.
  • Journal Title: Developmental Biology 295 Pages: 623-632
• [Presentation] Bisacylhydrazine ecdysone agonistと内在幼若ホルモンの相互作用による過剰脱皮誘導 (2009)
  • Author(s): 比留間潔・Lynn M. Riddiford・Stuart E. Reynolds
  • Organizer: 第53回日本応用動物昆虫学会
  • Place of Presentation: 北海道大学
• [Presentation] 実験形態学に始まり再び実験形態学へ (2009)
  • Author(s): 比留間潔
  • Organizer: 第53回日本応用動物昆虫学会
  • Place of Presentation: 北海道大学
• [Presentation] カイコcrochet細胞における形成能力のホルモン制御および細胞死のメカニズム (2009)
  • Author(s): 秋元真理・金児 雄・比留間 潔
  • Organizer: 第53回日本応用動物昆虫学会
  • Place of Presentation: 北海道大学
• [Presentation] コミットメントはall-or-noneで起こるか-Verson's glandを用いた単一細胞のコミットメント機構の解析 (2009)
  • Author(s): 村松大輔・比留間潔
  • Organizer: 第53回日本応用動物昆虫学会
  • Place of Presentation: 北海道大学
• [Presentation] カイコの転写因子BHR4発現と血中エクダイソン濃度との関連 (2009)
  • Author(s): 横山拓彦・比留間潔・冨田秀一郎
  • Organizer: 第53回日本応用動物昆虫学会
  • Place of Presentation: 北海道大学
• [Presentation] Bombyx neuropeptide GPCR transcriptome reveals authentic neuroendocrine network (2008)
  • Author(s): Yamanaka, N., -it-an, D., Kaneko, Y., Hiruma, K., Tanaka, Y., Shinoda, T., and Kataoka, H
  • Organizer: XXIII International Congress of Entomology
  • Place of Presentation: International Convention Centre, Durban, South Africa
• [Presentation] Hormonal control of pupal commitment of the epidermis of the silkworm (2008)
  • Author(s): Hiruma, K., and Muramatsu, D.
  • Organizer: Bombyx mori : comparison with that of Manduca sexta. Ecdysone Workshop 2008
  • Place of Presentation: Ulm, Germany
• [Presentation] 幼若ホルモンの形態形成抑制の役割とその栄養シグナルとの相互作用 (2008)
  • Author(s): 比留間潔・小山貴司・鈴木裕一郎・Riddiford, L.M.・Truman, J.W.
  • Organizer: 第52回日本応用動物昆虫学会
  • Place of Presentation: 宇都宮大学
• [Presentation] カイコ皮膚の幼虫-蛹コミットメントのホルモン制御 (2008)
  • Author(s): 村松大輔・比留間 潔
  • Organizer: 第52回日本応用動物昆虫学会
  • Place of Presentation: 宇都宮大学
• [Presentation] カイコcrochetにおけるdeathcommitmentの制御機構 (2008)
  • Author(s): 秋元真理・村松大輔・金児 雄・比留間 潔
  • Organizer: 第52回日本応用動物昆虫学会
  • Place of Presentation: 宇都宮大学
• [Presentation] カイコの転写因子BHR4の過剰発現による蛹化脱皮への影響 (2008)
  • Author(s): 横山拓彦・比留間潔・冨田秀一郎
  • Organizer: 第52回日本応用動物昆虫学会
  • Place of Presentation: 宇都宮大学
• [Presentation] カイコ培細胞株の幼若ホルモンに対する応答 (2008)
  • Author(s): 神村学・松本均・志村幸子・今西重雄・三田和英・篠田徹郎・金城輝則・比留間潔
  • Organizer: 第52回日本応用動物昆虫学会
  • Place of Presentation: 宇都宮大
• [Presentation] Hormonal control of pupal commitment of the epidermis of the silkworm, Bombyx mori : comparison with that of Manduca sexta (2008)
  • Author(s): Hiruma,K. and Muramatsu, D.
  • Organizer: Ecdysone Workshop 2008
  • Place of Presentation: University of Ulm(ドイツ)
• [Presentation] カイコcrochetにおけるdeath commitmentの制御機構 (2008)
  • Author(s): 秋元真理・村松大輔・金児 雄・比留間 潔
  • Organizer: 第52回 日本応用動物昆虫学会
  • Place of Presentation: 宇都宮大学
• [Presentation] カイコの転写因子BHR4の過剰発現による蛹化脱皮への影響 (2008)
  • Author(s): 横山拓彦・比留間潔・冨田秀一郎
  • Organizer: 第52回 日本応用動物昆虫学会
  • Place of Presentation: 宇都宮大学
• [Presentation] 幼若ホルモンの形態形成抑制の役割とその栄養シグナルとの相互作用 (2008)
  • Author(s): 比留間潔・小山貴司・鈴木裕一郎・Riddiford, L. M.・Truman, J. W.
  • Organizer: 第52回 日本応用動物昆虫学会
  • Place of Presentation: 宇都宮大学
• [Presentation] The alteration in the expression level of ecdysteroid-induced early gene (2007)
  • Author(s): Keshan, B., Hiruma, K., and Riddiford, L.M.
  • Organizer: E75, by juvenile hormone. Regional Symposium on Current Research Thrust in Animal Sciences
  • Place of Presentation: North-Eastern Hill University, India
• [Presentation] 成虫原器の形成と成長に及ぼす幼若ホルモンの役割 (2007)
  • Author(s): 比留間潔, Truman, J.W., Champlin, D.T., Riddiford, L.M.
  • Organizer: 日本応用動物昆虫学会第51回大会
• [Presentation] カイコ皮膚の培養系における幼虫-蛹コミットメントの制御 (2007)
  • Author(s): 村松大輔、金城輝則、金児雄、比留間潔
  • Organizer: 日本応用動物昆虫学会第51回大会
• [Presentation] 転写因子BHR3とBHR4カイコの発育と変態に及ぼす役割-遺伝子組み換えカイコの作成 (2007)
  • Author(s): 横山拓彦, 比留間潔, 冨田秀一郎
  • Organizer: 日本応用動物昆虫学会第51回大会
• [Presentation] Regulation of juvenile hormone biosynthesis by the corpora allata in Bombyx mori (2007)
  • Author(s): Hiruma, K., Kaneko, Y., Kinjoh, T., and Shinoda, T
  • Organizer: 9th International Conference on Juvenile Hormone
  • Place of Presentation: York, England
• [Presentation] Dopamine is a regulator of juvenile hormone biosynthesis by the corpora allata in Bombyx mori (2007)
  • Author(s): Kaneko, Y., and Hiruma, K
  • Organizer: 9th International Conference on Juvenile Hormone
  • Place of Presentation: York, England
• [Presentation] The morphostatic role of juvenile hormone and its interaction with nutrient-dependent signaling (2007)
  • Author(s): Truman, J.W., Koyama, T., Suzuki, Y., Hiruma, K., and Riddiford, L.M.
  • Organizer: 9th International Conference on Juvenile Hormone
  • Place of Presentation: York, England
• [Presentation] A direct role of JH in the control of imaginal disc formation and growth in Manduca. Fly Meeting (2006)
  • Author(s): Truman, J.W., Hiruma, K., Allee, J.P., Mac Whinnie, S.G.B., Champlin, D., and Riddiford, L. M
  • Organizer: 2006 Ecdysone Workshop Wednesday
  • Place of Presentation: Lanier Grand Ballroom A, Hilton Americas, Houston, TX.
  • Year and Date: 2006-03-29
• [Presentation] An overview of the molecular aspect of Manducaendocrinology (2006)
  • Author(s): Hiruma, K.
  • Organizer: Japan-USA Joint Symposium. Hornworms as Experimental Insects : In Retrospect and Prospect
  • Place of Presentation: Tokyo Silk Hall
  • Year and Date: 2006-01-28
• [Presentation] Regulation of enzymes involved in juvenile hormone biosynthesis (2006)
  • Author(s): Hiruma, K
  • Organizer: The 54th Annual Meeting of the Entomological Society of America
  • Place of Presentation: Indianapolis, USA
• [Presentation] Differential regulation of ecdysone-induced early gene E75 by juvenile hormone during molting and metamorphosis in the tobacco hornworm, Manduca sexta (2006)
  • Author(s): Keshan, B., Hiruma, K., and Riddiford, L.M.
  • Organizer: The 54th Annual Meeting of the Entomological Society of America
  • Place of Presentation: Indianapolis, USA
• [Presentation] エクダイソンに誘導される核レセプター、MHR3とE75B、は転写因子MHR4の調節因子であると共に、dopa decarboxylase発現の阻害因子である (2006)
  • Author(s): 比留間潔, Riddiford, L.M
  • Organizer: 日本応用動物昆虫学会第50回大会
  • Place of Presentation: 筑波大学
• [Presentation] A direct role of JH in the control of imaginal disc formation and growth in Manduca (2006)
  • Author(s): Truman, J.W., Hiruma, K., Allee, J.P., Mac Whinnie, S.G.B., Champlin, D., and Riddiford, L.M.
  • Organizer: The 5th International Symposium on Molecular Insect Science
  • Place of Presentation: Tucson, AZ
• [Presentation] The coordination of the sequential appearance of MHR4 and dopa decarboxylase during the decline of the ecdysteroid titer at the end of the molt (2006)
  • Author(s): Hiruma, K., and Riddiford, L.M
  • Organizer: The 16^<th> International Ecdysone Workshop
  • Place of Presentation: Ghent, Belgium
• [Presentation] Cloning and expression analysis of juvenile hormone biosynthetic enzymes and E75 isoforms in the silkworm, Bombyx mori (2006)
  • Author(s): Shinoda, T., Kinjoh, T., Mita, K., and Hiruma, K.
  • Organizer: The 7th International workshop on Molecular Biology and Genetics of the Lepidoptera
  • Place of Presentation: Kolympari, Crete, Greece
• [Presentation] Manduca sexta皮膚におけるmoling 遺伝子の発現誘導機構 (2006)
  • Author(s): 水口智江可、比留間潔、Riddiford, L.M.
  • Organizer: 昆虫ワークショップ06金沢
  • Place of Presentation: 石川県青少年総合研修センター
• [Presentation] Regulation of the ecdysone-induced cascade of transcription factors that lead to the induction of dopa decarboxylase expression (2005)
  • Author(s): Hiruma, K.
  • Organizer: NIAS/COE International Symposium : Present Status of Studies for Utilization of Insect Properties
  • Place of Presentation: Tsukuba International Congress Center
• [Presentation] The ecdysone-induced early gene E75 in Manduca sexta : its developmental regulation and hormonal control in epidermis and developing wing (2005)
  • Author(s): Keshan, B., Hiruma, K., and Riddiford, L.M.
  • Organizer: Frontiers in Molecular Endocrinology
  • Place of Presentation: Hyderabad, India