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Molecular mechanism of gene targeting in insect cells and its applications

Research Project

Project/Area Number 17380037
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionKyushu University

Principal Investigator

KUSAKABE Takahiro  Kyushu University, Graduate School of Agriculture, Associate Professor (30253595)

Co-Investigator(Kenkyū-buntansha) KAWAGUCHI Yutaka  Kyushu University, Graduate School of Agriculture, Professor (80038306)
LEE Jaeman  Kyushu University, Graduate School of Agriculture, Assistant Professor (50404083)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,680,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2007: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2006: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2005: ¥5,800,000 (Direct Cost: ¥5,800,000)
KeywordsSILKWORM / TARGETING / HOMOLOGOUS RECOMBINATION / INSECT / GENE DISRUPTION / CHROMATIN / 相同組換え
Research Abstract

Gene targeting is a method to manipulate a specific genomic loci by using homologous recombination that is one of an intrinsic DNA repair system. Since only the target gene can be disrupted precisely, this is a faithful method as compared with RNAi.
In this project, to improve the gene targeting efficiency, we constructed in vivo homologous recombination (HR) assay system and screened the genes related to HR in insect cells. As a result, we isolated silkworm AGO2 gene as a suppressor of HR at an extra-chromosomal DSB site. Furthermore, our results suggested that the Ago2 protein, at least in part, plays an important role to discriminate whether DSB is on the chromosome or not, through chromatin.
Consequently, we isolated and characterized chromatin regulators from silkworm cells to utilize the improvement of gene targeting efficiency. In silkworm chromatin, histone H3K9 was possible to be mono-, di-, or tri-methylated by. Su(Var)3-9. Furthermore, methylated H3K9 was recognized by HP1a and HP1b. Compared with other organisms, recruitment of the HP1a to the 5'-flanking region, however, is less effective to induce the transcription repression via hetrochromatin formation.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (108 results)

All 2008 2007 2006 2005

All Journal Article (64 results) (of which Peer Reviewed: 29 results) Presentation (44 results)

  • [Journal Article] Construction of gene expression systemsin in sect cell lines using proomoters from the silkworm, Bombyx mori2008

    • Author(s)
      Lee, et. al.
    • Journal Title

      J. Bioteclmol. 133

      Pages: 9-17

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori2008

    • Author(s)
      Lee, J., Takahashi, M., Mon, H., Nakajima, Y., Koga, K., Kawaguchi, Y., Kusakabe, T.
    • Journal Title

      J.Biotechnol 133

      Pages: 9-17

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Construction of gene expression systems in insect cell lines using promoters from the silkworm,Bombyx mori2008

    • Author(s)
      Lee, et. al.,
    • Journal Title

      J.Biotechnol. 133

      Pages: 9-17

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterotrimeric Complex of Replication Protein A Protein, from the. Silkworm, Bombyx mori2007

    • Author(s)
      Sugahara, et. al.
    • Journal Title

      J. Insect Biotech. Seric. 76

      Pages: 129-135

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Molecular Cloning of Silkworm Cdc37 and its Interaction with Hsp90 Chaperon2007

    • Author(s)
      Yamashita, et. al.
    • Journal Title

      J. Insect Biotech. Seric. 76

      Pages: 137-143

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Screening of high-permissive silkworm strains for efficient recombinant protein production in Autographa californica nuclear polyhedrosis virus (AcNPV)2007

    • Author(s)
      Lee, et. al.
    • Journal Title

      J. Insect Biotech. Seric. 76

      Pages: 101-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The gacA gene of Pseudomonas aeruginosa PAO1 is not required for full virulence in Bombvx mori2007

    • Author(s)
      Chieda, et. al.
    • Journal Title

      J. Insect Biotech. Seric. 76

      Pages: 86-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of inactivation of the superoxide dismutase genes on the virulence of Pseudomonas aeruginosa PAO1 to silkworm, Bombvx mori2007

    • Author(s)
      Iiyama, et. al.
    • Journal Title

      Appl. Environ. Microbiol 73

      Pages: 1569-1575

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Characteristic of Se, the white-sided egg mutation in Bombyx mori2007

    • Author(s)
      Kawaguchi, et. al.
    • Journal Title

      J. Insect Biotech. Seric 76

      Pages: 71-78

    • NAID

      130004935561

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis2007

    • Author(s)
      Zhang, et. al.
    • Journal Title

      J. Proteome res. 6

      Pages: 2295-2303

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Characteristic of Se the white-sided egg mutation in Bombyx mori2007

    • Author(s)
      Kawaguchi, Y., Yoshida, Y., Kusakabe, T., Lee, J., Koga, K.
    • Journal Title

      J.Insect Biotech. Seric 76

      Pages: 71-78

    • NAID

      130004935561

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The gacA gene of Pseudomonas aerugmosa PA01 is not required for full virulence in Bombyx mori. J.Insect Biotech.Seric.76, 86-962007

    • Author(s)
      Chicda, Y., Iiyama, K., Lee, J., Kusakabe, T., Yasunaga-Aoki, C., Lee, J., Kusakabe, T., Shimizu, S.
    • Journal Title

      J.Insect Biotech. Serie 76

      Pages: 86-96

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of inactivation of the superoxide dismutase genes on the virulence of Pseudomonas aeruginosa PAO1 to silkwonn, Bombyx mori2007

    • Author(s)
      Iiyama, K., Chieda, Y., Lee, J., Kusakabe, T., Yasunaga-Aoki, C., Lee, J., Kusakabe, T., Shimizu, S.
    • Journal Title

      Appl.Environ. Microbiol 73

      Pages: 1569-1575

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Screening of high-permissive silkworm strains for efficient recombinant protein production in Autographa californica nuclear polyhedrosis virus (AcNPV)2007

    • Author(s)
      Lee, J., Mon, H., Takahashi, M., Kawakami, N., Aoki, Y., Yoshida, Y., Banno, Y., Koga, K., Kawaguchi, Y., Kusakabe, T.
    • Journal Title

      J.Insect Biotech. Seric 76

      Pages: 101-106

    • NAID

      130004935558

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis2007

    • Author(s)
      Zhang, P., Aso, Y., Jikuya, H., Kusakabe, T., Lee, J., Kawaguchi, Y., Yamamoto, K., Banno, Y., Fujii, H.
    • Journal Title

      J.Proteome res 6

      Pages: 2295-2303

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Molecular Cloning of Silkworm Cdc37 and its Interaction with Hsp90 Chaperon2007

    • Author(s)
      Yamashita, J., Miyagawa, Y., Sugahara, R., Mon, H., Mitsunobu, H., Lee, J., Kawaguchi, Y., Kusakabe, T.
    • Journal Title

      J.Insect Biotech.Seric 76

      Pages: 137-143

    • NAID

      10019767230

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Heterotrimeric Complex of Replication Protein A, a Single-Stranded DNABinding Protein, from the Silkworm, Bombyx mori2007

    • Author(s)
      Sugahara, R., Mon, H., Yamashita, J., Mitsunobu, H., Lee, J., Kawaguchi, Y., Koga, K., Kusakabe, T.
    • Journal Title

      J.Insect Biotech.Seric 76

      Pages: 129-135

    • NAID

      10019767207

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Heterotrimeric Complex of Replication Protein A,a Single-Stranded DNABinding Protein,from the Silkworm,Bombyx mori2007

    • Author(s)
      Sugahara, et. al.
    • Journal Title

      J.Insect Biotech.Seric. 76

      Pages: 129-135

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Cloning of Silkworm Cdc37 and its Interaction with Hsp90 Chaperon2007

    • Author(s)
      Yamashita, et. al.,
    • Journal Title

      J.Insect Biotech.Seric. 76

      Pages: 137-143

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Screening of high-permissive silkworm strains for efficient recombinant protein production in Autographa californica nuclear polyhedrosis virus(AcNPV)2007

    • Author(s)
      Lee, et. al.
    • Journal Title

      J.Insect Biotech.Seric. 76

      Pages: 101-106

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The gacA gene of Pseudomonas aeruginosa PAO1 is not required for full virulence in Bombyx mori2007

    • Author(s)
      Chieda, et. al.,
    • Journal Title

      J.Insect Biotech.Seric. 76

      Pages: 86-106

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of inactivation of the superoxide dismutase genes on the virulence of Pseudomonas aeruginosa PAO1 to silkworm,Bombyx mori2007

    • Author(s)
      Iiyama, et. al.,
    • Journal Title

      Appl.Environ.Microbiol 73

      Pages: 1569-1575

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristic of Se,the white-sided egg matation in Bombyx mori2007

    • Author(s)
      Kawaguchi, et. al.,
    • Journal Title

      J.Insect Biotech.Seric. 76

      Pages: 71-78

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic Profiling of the silkworm muscle proteins during larval-pupal metamorphosis2007

    • Author(s)
      Zhang, et. al.,
    • Journal Title

      J.Proteome res. 6

      Pages: 2295-2303

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Site-specific gene integeration in cultured silkworm cells mediated by fC31 integrase2006

    • Author(s)
      Nakayama G., et. al.
    • Journal Title

      Mol.Genet.Genomics 275

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cell Cycle Arrest induced by Radiation in Culutured Silkworm Cells2006

    • Author(s)
      Takahashi M., et. al.
    • Journal Title

      J.Insect Biotech.Seric. 75

      Pages: 23-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Micropylar structure of chorion of the female sterile mutation,bd, in,Bombyx mori2006

    • Author(s)
      Kawaguchi, Y., et. al.
    • Journal Title

      J.Insect Biotech.Seric. 75

      Pages: 9-14

    • NAID

      10017316105

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Role of the Silkworm Argonaute2 Homologue Gene in Double-Strand Break Repair of Extrachromosomal DNA2006

    • Author(s)
      Tsukioka H., et. al.
    • Journal Title

      Nucleic Acids Res. 34

      Pages: 1092-1101

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Radiation resistance and its inheritance in silkworm,Bombyx mori2006

    • Author(s)
      Takahashi M., et. al.
    • Journal Title

      J.Fac.agr., Kyushu Univ. 51

      Pages: 261-264

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Construction of gateway-based destination vectors for detecting subcellular localizatio n of proteins in the silkworm,Bonbyx mori2006

    • Author(s)
      Mitsunobu, H., et. al.
    • Journal Title

      J.Insect Biotech.Seric. 75

      Pages: 141-146

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Site-specific gene integration in culturedsilkworm cells mediated by fC31 integrase2006

    • Author(s)
      Nakayama, G., Kawaguchi, Y., Koga, K., Kusakabe, T.
    • Journal Title

      Mol. Genet.Genomics 275

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cell Cycle Arrest induced by Radiation in Cultured Silkworm Cells2006

    • Author(s)
      Takahashi, M., Lee, J., Mon, H., Kawaguchi, Y., Koga, K., Kusakabe, T.
    • Journal Title

      J.Insect Biotech.Seric 75

      Pages: 23-30

    • NAID

      10017316146

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Micropylar structure of cborjon of the female sterile mutation, bd, in, Bombyx mori2006

    • Author(s)
      Kawaguchi, Y., Kusakabe, T., Lee, J., Nakajima, Y., Koga, K.
    • Journal Title

      J.Insect Biotech.Seric 75

      Pages: 9-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Role of the Silkworm Argonaute2 Homologue Gene in Double-Strand Break Repair of Extrachromosomal DNA2006

    • Author(s)
      Tsukioka, H., Takahashi, M., Mon, H., Okano, K., Mita, K., Shimada, T., Lee, J., Kawaguchi, Y., Koga, K., Kusakabe, T.
    • Journal Title

      Nucleic Acids Res 34

      Pages: 1092-1101

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Radiation resistance and its inheritance in the silkworm, Bombyx mori2006

    • Author(s)
      Takahashi, M., Lee, J., Mon, H., Yoshida, H., Kawaguchi, Y., Maekawa, H., Koga, K., Kusakabe, T.
    • Journal Title

      J.Fac.Agr., (Kyushu Univ) 51

      Pages: 261-264

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Construction of gateway-based destination vectors for detecting subcellular localization of proteins in the silkworm, Bombyx mori2006

    • Author(s)
      Mitsunobu, H., Sakashita, K., Mon, H., Lee, J., Kawaguchi, Y., Koga, K., Kusakabe, T.
    • Journal Title

      J.Insect Biotech. Seric 75

      Pages: 141-146

    • NAID

      10020516432

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Site-specific gene integration in cultured silkworm cells mediated by ΦC31 integrase.2006

    • Author(s)
      Nakayama G.et al.
    • Journal Title

      Mol.Genet.Genomics 275

      Pages: 1-8

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cell Cycle Arrest induced by Radiation in Cultured Silkworm Cells.2006

    • Author(s)
      Takahashi M.et al.
    • Journal Title

      J.Insect Biotech.Seric. 75

      Pages: 23-30

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Micropylar structure of chorion of the female sterile mutation,bd,in,Bombyx mori.2006

    • Author(s)
      Kawaguchi Y.et al.
    • Journal Title

      J.Insect Biotech.Seric. 75

      Pages: 9-14

    • NAID

      10017316105

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Role of the Silkworm Argonaute2 Homologue Gene in Double-Strand Break Repair of Extrachromosomal DNA.2006

    • Author(s)
      Tsukioka H.et al.
    • Journal Title

      Nucleic Acids Res. 34

      Pages: 1092-1101

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Radiation resistance and its inheritance in the silkworm,Bombyx mori.2006

    • Author(s)
      Takahashi M.et al.
    • Journal Title

      J.Fac.Agr.,Kyushu Univ.51 51

      Pages: 261-264

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Construction of gateway-based destination vectors for detecting subcellular localization of proteins in the silkworm,Bombyx mori.2006

    • Author(s)
      Mitsunobu H.et al.
    • Journal Title

      J.Insect Biotech.Seric. 75

      Pages: 141-146

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Efficient nonviral gene transfer mediated by polyethylenimine in an insect cell line2005

    • Author(s)
      Maeda, T., et. al.
    • Journal Title

      J.Insect Biotech. Seric. 74

      Pages: 21-26

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Inheritance of gon, a body color mutation of newly hatched larvae in Bombyx mori2005

    • Author(s)
      Kawaguchi, Y., et. al.
    • Journal Title

      J.Insect Biotech. Seric. 74

      Pages: 35-37

    • NAID

      10015434007

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell lineBmN42005

    • Author(s)
      Ohsaki, A., et. al.
    • Journal Title

      Mol. Biol. Reports 32

      Pages: 25-34

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cloning and Characterization of a Ribonuclease L Inhibitor from the Silkworm,Bombyx mori2005

    • Author(s)
      Maeda, T., et. al.
    • Journal Title

      DNA Sequence 16

      Pages: 21-27

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Differential expression of a Bombyx mori AHA1 homologue during spermatogenesis2005

    • Author(s)
      Miyagawa, Y., et. al.
    • Journal Title

      Insect Mol. Biol. 14

      Pages: 245-253

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Evidence of defect in fibroin secretion in the posterior silk gland cells of the tic mutant of Bonbyx mori2005

    • Author(s)
      Sakaguchi, B., et. al.
    • Journal Title

      J.Insect Biotech. Seric. 74

      Pages: 75-78

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Efficient gene transfer into silkworm larval tissues by a combination of sonoporationand lipofection2005

    • Author(s)
      Lee, J., et. al.
    • Journal Title

      Cell Biol. Int. 29

      Pages: 976-979

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Efficient nonviral gene transfer mediated by polyethylenimine in an insect cell line2005

    • Author(s)
      Madea, T., Kusakabe, T., Lee, J., Miyagawa, Y., Kawaguchi, Y., Koga, k.
    • Journal Title

      J. Insect Biotech.Seric 74

      Pages: 21-26

    • NAID

      10015433963

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inheritance of gon, a body color mutation of newly hatched larvae in Bombyx mori2005

    • Author(s)
      Kawaguchi, Y., Doira, H., Ohta, K., Kusakabe, T., Koga K.
    • Journal Title

      J.Insect Biotech. Seric 74

      Pages: 35-37

    • NAID

      10015434007

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori2005

    • Author(s)
      Chieda, Y., Iiyama, K., Yasunaga-Aoki, C., Lee, J., Kusakabe, T., Shimizu, S.
    • Journal Title

      FEMS Microbiol. Lett 244

      Pages: 181-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN42005

    • Author(s)
      Ohsaki, A., Iiyama, K., Miyagawa, Y., Kawaguchi, Y., Koga, K., Kusakabe, T.
    • Journal Title

      Mol.Biol.Reports 32

      Pages: 25-34

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cloning and Characterization of a Ribonuclease L Inhibitor from the Silkworm, Bombyx Mori2005

    • Author(s)
      Maeda, T., Kusakabe, T., Lee, J., Miyagawa, Y., Kawaguchim, Y., Koga, K.
    • Journal Title

      DNA Sequence 16

      Pages: 21-27

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Differential expression of a Bombyx mori AHA1 homologue during spermatogenesis. Insect Mol.Biol.14, 245-2532005

    • Author(s)
      Miyagawa, Y., Lee, J., Maeda, T., Koga, K., Kawaguchi, Y., Kusakabe, T.
    • Journal Title

      Insect Mol.Biol 14

      Pages: 245-253

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Efficient gene transfcr into silkworm larval tissues by a combination of sonoporation and lipofection2005

    • Author(s)
      Lee, J., Takahashi, M., Mon, H., Koga, K., Kawaguchi, Y., Kusakabe, T.
    • Journal Title

      Cell Biol.Int 29

      Pages: 976-979

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Evidence of defect in fibroin secretion in the posterior silk gland cells of the flc mutant of Bombyx mori.J.Insect Biotech.Seric.74,75-782005

    • Author(s)
      Sakaguchi, B., Kawaguchi, Y., Koga, K., Kusakabe, T.
    • Journal Title

      J.Insect Biotech. Seric 74

      Pages: 75-78

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Efficient nonviral gene transfer mediated by polyethylenimine in an insect cell line.2005

    • Author(s)
      Maeda, T. et al.
    • Journal Title

      J.Insect Biotech.Seric. 74

      Pages: 21-26

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Inheritance of gon, a body color mutation of newly hatched larvae in Bombyx mori.2005

    • Author(s)
      Kawaguchi, Y. et al.
    • Journal Title

      J.Insect Biotech.Seric. 74

      Pages: 35-37

    • NAID

      10015434007

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN4.2005

    • Author(s)
      Ohsaki, A. et al.
    • Journal Title

      Mol.Biol.Reports 32

      Pages: 25-34

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cloning and Characterization of a Ribonuclease L Inhibitor from the Silkworm, Bombyx mori.2005

    • Author(s)
      Maeda, T. et al.
    • Journal Title

      DNA Sequence 16

      Pages: 21-27

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Differential expression of a Bombyx mori AHA1 homologue during spermatogenesis.2005

    • Author(s)
      Miyagawa, Y. et al.
    • Journal Title

      Insect Mol.Biol. 14

      Pages: 245-253

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Evidence of defect in fibroin secretion in the posterior silk gland cells of the flc mutant of Bombyx mori.2005

    • Author(s)
      Sakaguchi, B. et al.
    • Journal Title

      J.Insect Biotech.Seric. 74

      Pages: 75-78

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection.2005

    • Author(s)
      Lee, J.et al.
    • Journal Title

      Cell Biol.Int. 29

      Pages: 976-979

    • Related Report
      2005 Annual Research Report
  • [Presentation] カイコにおける融合型GAL4転写因子の利用2007

    • Author(s)
      小林, ら
    • Organizer
      日本分子生物学会本会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] カイコにおけるHPlaとメチル化ピストンH3の相互作用2007

    • Author(s)
      光延, ら
    • Organizer
      日本分子生物学会本会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] カイコのヘテロクロマチン形成におけるζストンメチルトランスフェラーゼSu(var)3-9の機能解析2007

    • Author(s)
      竹野, ら
    • Organizer
      日本分子生物学会本会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] カイコにおけるMnd1とHop2の機能解析2007

    • Author(s)
      戸畑, ら
    • Organizer
      日本分子生物学会本会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] カイコにおけるFA複合体の解析2007

    • Author(s)
      管原, ら
    • Organizer
      日本分子生物学会本会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] カイコBrca2の機能解析2007

    • Author(s)
      門, ら
    • Organizer
      日本分子生物学会本会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] カイコのヘテロクロマチン形成におけるヒストンメチルトランスフェラーゼSu(var)3-9の機能解析2007

    • Author(s)
      竹野, ら
    • Organizer
      日本分子生物学会本会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] Molecular cloning and characterization of HPla, HPlb in the sillcworm, Bombyx mori2007

    • Author(s)
      Mitsunobu, et. al.
    • Organizer
      International congress of insect biotechnology & industry
    • Place of Presentation
      Daegu; Korea
    • Year and Date
      2007-08-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Moleculer Cloning of Piwi and Aubergine homolog genes from the silkworm, Bombyx mori2007

    • Author(s)
      Tatsuke, et. al.
    • Organizer
      International congress of insect biotechnology & industry
    • Place of Presentation
      Daegu; Korea
    • Year and Date
      2007-08-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular cloning and characterization of HP1a,HP1b in the silkworm,Bombyx mori2007

    • Author(s)
      Mitsunobu, et. al.
    • Organizer
      International congress of insect biotechnology & industry
    • Place of Presentation
      Daegu;Korea
    • Year and Date
      2007-08-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] Moleculer Cloning of Piwi and Aubergine homolog genes from the silkworm,Bombyx mori2007

    • Author(s)
      Tatsuke, et. al.
    • Organizer
      International congress of insect biotechnology & industry
    • Place of Presentation
      Daegu;Korea
    • Year and Date
      2007-08-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] カイコにおける相同組換えに関わる遺伝子の機能解析2007

    • Author(s)
      門, ら
    • Organizer
      蚕糸学会本会
    • Place of Presentation
      茨城
    • Year and Date
      2007-04-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] カイコHPlaの機能解析2007

    • Author(s)
      光延, ら
    • Organizer
      蚕糸学会本会
    • Place of Presentation
      茨城
    • Year and Date
      2007-04-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] カイコにおけるFANCLのクローニングと機能解析2007

    • Author(s)
      管原, ら
    • Organizer
      蚕糸学会本会
    • Place of Presentation
      茨城
    • Year and Date
      2007-04-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 高効率的組換えタンィくク質発現用宿主としてのカイコ系統の開発2007

    • Author(s)
      李, ら
    • Organizer
      蚕糸学会本会
    • Place of Presentation
      茨城
    • Year and Date
      2007-04-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 高効率的組換えタンパク質発現用宿主としてのカイコ系統の開発2007

    • Author(s)
      李, ら
    • Organizer
      蚕糸学会本会
    • Place of Presentation
      茨城
    • Year and Date
      2007-04-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] Reciprocal Expression of the Bombyx innexin gene family.Sun Mee Hong, Si Kab Nho, Seok Woo Kang, Jae Man Lee2007

    • Author(s)
      Sun Mee, Hong, Si Kab, Nho, Seok Woo, Kang, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      International Congress of Insect Biotechnology & Industry
    • Place of Presentation
      Daegu ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular cloning and characterization of HP1a, HP1b in the silkworm, Bombyx mori. Hitoshi Mitsunobu, Hiroaki Mon, Jae Man Lee2007

    • Author(s)
      Hitoshi, Mitsunobu, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      International Congress of Insect Biotechnology & Industry
    • Place of Presentation
      Daegu ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Moleculer Cloning of Piwi and Aubergine homolog genes from the silkworm, Bombyx mori.Tsuneyuki Tatsuke, Kousuke Sakashita, Ryouhei Sugahara, JaeMan Lee2007

    • Author(s)
      Tsuneyuki, Tatsuke, Kousuke, Sakashita, Ryouhei, Sugahara, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      International Congress of Insect Biotechnology & Industry
    • Place of Presentation
      Daegu ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular cloning and characterization of histone methyltransferase Su(var)3-9 in Bombyx mori2007

    • Author(s)
      Natsumi, Takeno, Hitoshi, Mitsunobu, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      International Congress of Insect Biotechnology & Industry
    • Place of Presentation
      Daegu ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Virulence of phospholipase C mutants of Psudomonas Aeruginosa against Bombyx mori2007

    • Author(s)
      Kazuhiro, Iiyama, Yuuka, Chieda, Jae Man, Lee, Takahiro, Kusakabe Chisa, Yasunaga-Aoki, Susumu, Shimizu
    • Organizer
      International Congress of Insect Biotechnology & Industry
    • Place of Presentation
      Daegu ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Exotoxin is an important virulence factor of Pseudomonas aeruginosa against Bombyx mori2007

    • Author(s)
      Yuuka, Chieda, Kazuhiro, liyama, Jaeman, Lee, Takahiro, kusakabe, Chisa, Yasunaga-Aoki, Susumu, Shimizu
    • Organizer
      International Congress of Insect Biotechnology & Industry
    • Place of Presentation
      Daegu ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Characterizing of novel protein similar to HSF(heat shock transcription factor) in the silkworm2007

    • Author(s)
      Bombyx, Mori
    • Organizer
      International Congress of Insect Biotechnology & Industry
    • Place of Presentation
      Daegu ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA repair and recombination in insect cells2006

    • Author(s)
      Takahiro, Kusakabe, Hiroaki, Mon, Haruna, Tsukioka, Lee, JaeMan, Yutaka, Kawaguchi
    • Organizer
      International Symposium on Insect Genetics & Genomics
    • Place of Presentation
      Hyderabad ; India
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Double-strand break repair by homologous recombination in silkworm cells2006

    • Author(s)
      Hiroaki, Mon, Ryohei, Sugahara, JaeMan, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto ; Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular cloning and characterization of HP1a, HP1b in the silkworm, Bombyx mori2006

    • Author(s)
      Hitoshi, Mitsunobu, Hiroaki, Mori, Masateru, Takahashi, Jac Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A proteomic approach to silkworm proteins responding to g-irradiation2006

    • Author(s)
      Hiroko, Yoshida, Masateru, Takahashi, Pingbo, Zhang, Yoshinon, Ueno, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahtro, Kusakabe
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto ; Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular cloning and characterization of FANCD2 in Bombyx mori Ryohei Sugahara2006

    • Author(s)
      Hiroaki, Mon, Jae Man, Lee, Takahiro, Kusakabe, Yutaka, Kawaguchi
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto ; Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular cloning and characterization of HSP90 cochaperones in the silkworm Bombyx mori2006

    • Author(s)
      Jun, Yamashita, Yoshitaka, Miyagawa, Hiroaki, Mon, Hitoshi, Mistunobu, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto ; Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cellular response to DNA double strand beraks(DSBs) in cultured silkworm cells2006

    • Author(s)
      Takahiro, Kusakabe, Hiroaki, Mon, Masateru, Takahashi, JaeMan, Lee, Yutaka, Kawaguchi
    • Organizer
      The Workshop on Lepidopteran Molecular Biology and Genetics
    • Place of Presentation
      Crete ; Greek
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Double-strand break repair by homologous recombination in silkworm cells2006

    • Author(s)
      Hiroaki, Mori, Ryohei, Sugahara, JaeMan, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      The Workshop on Lepidopteran Molecular Biology and Genetics
    • Place of Presentation
      Crete ; Greek
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A Large Amount of dsRNA affects on Gene Expression in Cultured Silkworm Cells2006

    • Author(s)
      Kousuke, Sakashita, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      Asta-Pacific Congress of Sericulture & Insect Biotechnology
    • Place of Presentation
      Sangju ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Proteomic Analysis of Silkworm Proteins Responding to DNA Damage Induced by Ionizing Radiation2006

    • Author(s)
      Hiroko, Yoshida, Masateru, Takahashi, Hiroaki, Mon, Pingbo, Zhang, Yoshinori, Ueno, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      Asia-Pacific Congress of Sericulture & Insect Biotechnology
    • Place of Presentation
      Sangiu ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular Cloning and Functional Characterization of the Fanconi Anemia Protein, FANCD2 in Bombyx mori2006

    • Author(s)
      Ryohei, Sugahara, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      Asia-Pacific Congress of Sericulture & Insect Biotechnology
    • Place of Presentation
      Sangiu ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular Cloning and Characterization of Hsp70, Hsc70-4, and Hsp40 in the silkworm2006

    • Author(s)
      Bombyx, mori Jun, Yamashita, Yoshitaka, Miyagawa, Hitoshi, Mitsunobu, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      Asia-Pacific Congress of Sericulture & Insect Biotechnology
    • Place of Presentation
      Sangiu ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Bombyx mori as a model host for Pseudomonas aerugmosa infection2006

    • Author(s)
      Kazuhiro, Yuuka, Chieda, Jaeman, Lee, Takahiro, kusakabe, Chisa, Yasunaga-Aoki, Susumu, Shimizu
    • Organizer
      Asia-Pacific Congress of Sericulture & Insect Biotechnology
    • Place of Presentation
      Sangju ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] uvrC is necessary for Pseudomonas aeruginosa PAO1 to be highly virulent in Bombyx mori2006

    • Author(s)
      Yuuka, Chieda, Kazuhiro, Iiyama, Jaeman, Lee, Takahiro, kusakabe, Chisa, Yasunaga-Aoki, Susumu, Shimizu
    • Organizer
      Asia-Pacific Congress of Sericulture & Insect Biotechnology
    • Place of Presentation
      Sangiu ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Comparison of productive and non-productive Bombyx mori nucleopolyhedrovirus infection in selected insect cell lines2006

    • Author(s)
      Naoya, Kawakami, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      Asia-Pacific Congress of Sericulture & Insect Biotechnology
    • Place of Presentation
      Sangm ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Establishment of fluorescent proteins-fusion systems in the silkworm, Bombyx mori2005

    • Author(s)
      Hitoshi, Mitsunobu, Hiroaki, Mon, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      5th Asia-Pacific Congress of Entomology
    • Place of Presentation
      Jeju ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Comparison of gene transfer efficiency into insect cells and tissues with different methods2005

    • Author(s)
      Jae Man, Lee, Masateru, Takahashi, Hiroaki, Mon, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      5th Asia-Pacific Congress of Entomology
    • Place of Presentation
      Jeju ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Double strand break repair by homologous recombination in silkworm cells2005

    • Author(s)
      Hiroaki, Mon, Ryohei, Sugahara, Takahiro, Kusakabe, Yutaka, Kawaguchi
    • Organizer
      5th Asia-Pacific Congress of Entomology
    • Place of Presentation
      Jeju ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Construction of silkworm gene silencing systems using bacurovirus mediated gene transfer2005

    • Author(s)
      Yoshito, Kojin, Jae Man, Lee, Takahiro, Kusakabe, Yutaka, Kawaguchi
    • Organizer
      5th Asia-Pacific Congress of Entomology
    • Place of Presentation
      Jeju ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Construction of Tet-on gene expression system in silkworm, Bombyx mori2005

    • Author(s)
      Noriko, Karasaki, Hiroaki, Mon, Takahiro, Kusakabe, Yutaka, Kawaguchi, Katumi, Koga
    • Organizer
      5th Asia-Pacific Congress of Entomology
    • Place of Presentation
      Jeju ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The expression profiles of silkworm RAD51 and DMC1 genes during the process of meiotic recombination2005

    • Author(s)
      Yukiko, Ishikawa, Yoshiaki, Kosaka, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
    • Organizer
      5th Asia-Pacific Congress of Entomology
    • Place of Presentation
      Jeju ; Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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