| Project/Area Number |
17380042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Hokkaido University |
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Principal Investigator |
SHINANO Takuro Hokkaido University, Research Faculty of Agriculture., Associate Professor (20235542)
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| Co-Investigator(Kenkyū-buntansha) |
OKAZAKI Keiki National Agricultural Research Center for Hokkaido Region, 農業生物系特定産業技術研究機構・北海道農業研究センター, Researcher (40414750)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥15,110,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2006: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥6,700,000 (Direct Cost: ¥6,700,000)
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| Keywords | Metabolome / GC-MS / Cellular organelle / Gramineae / Leguminosae / Perimary photosynthate / LC-MS / MS / 一次代謝産物 / フラボノイド / UPLC-MS |
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| Research Abstract |
When comparing the behavior of primary photosynthate between rice and soybean, a large amount of primary photosynthate is distributed to starch fraction especially in soybean. As the metabolic pathway regarding photosynthesis, glycolysis, TCA cycle, etc. are same between rice and soybean, the observed difference is assumed to be derived from the different size of the pool of assimilated compounds. To confirm the idea, several genes' expression which are working on metabolites partitioning are investigated at different growth stage. The obtained results support the photosynthate distribution pattern. A large distribution of primary phosynhtate into starch fraction indicate the importance of delivery system among cytosol, chloroplast, and vacuole, thus a new method is developed to separate these organelle without further enzyme reaction during extraction process. Non aqueous fractionation method was developed and applied to soybean leaf. Each fraction was further analyzed by metabolomics approach using gas chromatography mass spectrometry (GC-MS). The metabolomics approach allows us to identify and quantify about 130 compounds in the tissue.
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