Eco-genomics of microbial denitrification in agricultural fields : N_2O paradox in rhizobial symbiosis with legumes and soil community genomics

• Project/Area Number: 17380046
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Tohoku University
• Principal Investigator: MINAMISAWA Kiwamu Tohoku University, 大学院・生命科学研究科, 教授 (70167667)
• Co-Investigator(Kenkyū-buntansha): KEISHI Senoo 東京大学, 大学院・農学生命研究科, 教授 (40206652)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,500,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥2,100,000); Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2007: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2006: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 2005: ¥3,800,000 (Direct Cost: ¥3,800,000)
• Keywords: 根粒菌 / 脱窒 / 亜酸化窒素 / 硝酸 / 土壌 / ダイズ / nosZ / 老化 / 多型解析 / 根圏 / napA / ダイズ根粒菌 / 安定同位体

Research Abstract

土壌のない実験室系では根粒はN_2Oを吸収か非発生であるが、圃場ダイズ根粒はN_2Oの発生源であった。圃場を模した土壌栽培系とダイズ根粒菌変異株の実験より、nosZを欠損株は硝酸からN_2O発生を起こし、その硝酸は硝化細菌により供給されていた。ゲノム比較等により農耕地土壌のBJ_2クラスターに属するダイズ根粒菌は、nosZを欠損し、最終産物としてN_2Oを生成した。以上の結果は、少なくともダイズ根粒菌のnosZ遺伝子型と硝化細菌の共同作用により圃場ダイズ根粒からN2_O発生が生ずることを示唆している。

Research Products

• [Journal Article] Genomic comparison of Bradyrhizobium japonicum strawith different symbiotic nitrogen-fixing capabilities and other Bradyrhizobiaceae members (2009)
  • Author(s): Itakura, M., K. Saeki, H. Omori, T. Yokoyama, T. Kaneko, S. Tabata, T. Ohwada, S.Tajima, T. Uchiumi, K. Honnma, K. FujitaIwata, Y. Saeki, Y. Hara, S. Ikeda, S. Eda, H. Mitsui and K. Minamisawa
  • Journal Title: ISME J. 3 Pages: 326-339
• [Journal Article] Nitrous oxide emission and microbial community in the rhizosphere of nodulated soybeans during the late growth period (2009)
  • Author(s): Inaba, S., K. Tanabe, S. Eda, S. Ikeda, A. Higashitani, H. Mitsui, and K. Minamisawa
  • Journal Title: Microbes Environ 24 Pages: 64-67
  • NAID: 110007131141
• [Journal Article] Genomic comparison of Bradyrhizobium japonicum strains with different symbiotic nitrogen-fixing capabilities and other Biadyrhi zobiaceae members (2009)
  • Author(s): Itakura, M.
  • Journal Title: ISME J 3 Pages: 326-339
  • Peer Reviewed
• [Journal Article] Nitrous oxide emission and microbial community in the rhizosphere of nodulated soybeans during the late growth period (2009)
  • Author(s): Inaba, S.
  • Journal Title: Microbes Environ 24(印刷中)
  • Peer Reviewed
• [Journal Article] Microbial community analysis of field-grown soybeans with different nodulation phenotypes (2008)
  • Author(s): Ikeda, S., L.E.E.Rallos, S. Inaba, S. Eda, H. Mitsui, and K. Minamisawa
  • Journal Title: Appl.Environ. Microbiol 74 Pages: 5704-5709
• [Journal Article] Generation of Bradyrhizobium japonicum mutants with increased N_20 reductase activity by selection after introduction of a mutated dnaQ gene (2008)
  • Author(s): Itakura, M., K. Tabata, S. Eda, H. Mitsui, K. Murakami, J. Yasuda, and K. Minamisawa
  • Journal Title: Appl.Environ. Microbiol 74 Pages: 7258-7264
• [Journal Article] ゲノム情報に基づいた植物共生細菌の環境応答と物質循環機能の解明 (2008)
  • Author(s): 南澤究、増田幸子、板倉学、池田成志
  • Journal Title: 土と微生物 62 Pages: 89-92
• [Journal Article] Microbial community analysis of field-grown soybeans with different nodulation phenotypes (2008)
  • Author(s): Ikeda, S.
  • Journal Title: Appl. Environ. Microbiol 74 Pages: 5704-5709
  • Peer Reviewed
• [Journal Article] Generation of Bradyrhizobium japonicum mutants with increased N 2O reductase activity by selection after introduction of a mutated dnaQ gene (2008)
  • Author(s): Itakura, M.
  • Journal Title: Appl. Environ. Microbiol 74 Pages: 7258-7264
  • Peer Reviewed
• [Journal Article] Microbial community analysis of the phytosphere using culture-independent methodologies (2007)
  • Author(s): Saito, A., S. Ikeda, H. Ezura, and K. Minamisawa
  • Journal Title: Microbes Environ 22(2) Pages: 93-105
  • NAID: 110006290371
• [Journal Article] RISA法による微生物多様性評価 (2007)
  • Author(s): 齋藤朝美、池田成志、則武ちあき、赤坂真理子、藤城圭輔、安藤勝彦、南澤究
  • Journal Title: 日本微生物生態学会誌 22(2) Pages: 59-71
  • NAID: 110006381189
• [Journal Article] A Mesorhizobium loti mutant with reduced glucan content shows defective invasion of its host plant Lotus japonicus. (2007)
  • Author(s): Kawaharada Y.
  • Journal Title: Microbiology 153 Pages: 3983-3993
  • Peer Reviewed
• [Journal Article] Microbial community analysis of the phytosphere using culture-independent methodologies. (2007)
  • Author(s): Saito, A.
  • Journal Title: Microbes Environ. 22 Pages: 93-105
  • Peer Reviewed
• [Journal Article] Rhizobitoxine-induced chlorosis occurs in coincidence with methionine deficiency in soybeans. (2007)
  • Author(s): Okazaki, S.
  • Journal Title: Botany 100 Pages: 55-59
  • Peer Reviewed
• [Journal Article] Correlation of denitrifying capability with the existence of nap, nir, nor and nos genes in diverse strains of soybean bradyrhizobia (2006)
  • Author(s): Sameshima-Saito, R., K. Chiba, and K. Minamisawa
  • Journal Title: Microbes Environ 21(3) Pages: 174-184
  • NAID: 110004809525
• [Journal Article] Symbiotic Bradyrhizobium japonicum reduces N_2O surrounding the soybean root system via nitrous oxide reductase (2006)
  • Author(s): Sameshima-Saito, R., K. Chiba, J. Hirayama, M. Itakura, H. Mitsui, S. Eda, and K. Minamisawa
  • Journal Title: Appl. Environ. Microbiol 72 Pages: 2526-2532
• [Journal Article] 耕地からの地球温暖化ガスN_2O発生とその抑制 (2006)
  • Author(s): 南澤究、犬伏和之
  • Journal Title: 水環境学会誌 Vol.29, No.2 Pages: 67-71
• [Journal Article] Rhizobitoxine modulates plant-microbe interactions by ethylene inhibition (2006)
  • Author(s): Sugawara, M., S.Okazaki, N.Nukui, H.Ezura, H.Mitsui, K.Minamisawa
  • Journal Title: Biotechnology Advances. 24 Pages: 382-388
• [Journal Article] Evaluation of nitrogen fixation capability of endophytic clostridia by acetylene reduction and reverse transcription-PCR targeted to nifH transcript and ribosomal RNA. (2006)
  • Author(s): Saito, A., K.Minamisawa
  • Journal Title: Microbes Environ. 21 Pages: 23-35
• [Journal Article] Symbiotic Bradyrhizobium japonicum reduces N_2O surrounding the soybean root system via nitrous oxide reductase. (2006)
  • Author(s): Sameshima-Saito, R., K.Chiba, J.Hirayama, M.Itakura, H.Mitsui, S.Eda, K.Minamisawa
  • Journal Title: Appl. Environ. Microbiol. 72 Pages: 2526-2532
• [Journal Article] Expression of 1-aminocyclopropane-1-carboxylic acid deaminase gene expression requires symbiotic nitrogen-fixing regulator gene nifA2 in Mesorhizobium loti MAFF303099. (2006)
  • Author(s): Nukui, N., K.Minamisawa, S.Ayabe, T.Aoki
  • Journal Title: Appl. Environ. Microbiol. 72 Pages: 4964-4969
• [Journal Article] Correlation of denitrifying capability with the existence of nap, nir, nor and nos genes in diverse strains of soybean bradyrhizobia (2006)
  • Author(s): Sameshima-Saito, R., K.Chiba, K.Minamisawa
  • Journal Title: Microbes Environ. 21 Pages: 174-184
  • NAID: 110004809525
• [Journal Article] A milestone for endophyte biotechnology (2006)
  • Author(s): Minamisawa, K.
  • Journal Title: Nature Biotechnology 24 Pages: 1357-1358
• [Journal Article] Symbiotic Bradyrhizobium japonicum reduces N_2O surrounding the soybean root system via nitrous oxide reductase (2006)
  • Author(s): Sameshima-Saito, R., K.Chiba, J.Hirayama, M.Itakura, H.Mitsui, S.Eda, K.Minamisawa
  • Journal Title: Appl.Environ.Microbiol. 72(in press)
• [Journal Article] Rhizobitoxine modulates plant-microbe interactions by ethylene inhibition. (2006)
  • Author(s): Sugawara, M., Okazaki, S., Nukui, N., Ezura, H., Mitusi, H., Minamisawa, K.
  • Journal Title: Biotechnology Advances. (in press)
• [Journal Article] Soil microbial community analysis in the environmental risk assessment of transgenic plants (2006)
  • Author(s): Ikeda, S., N.Ytow, H.Ezura, K.Minamisawa, T.Fujimura
  • Journal Title: Plant Biotechnology (in press)
  • NAID: 10021908153
• [Journal Article] Evaluation of nitrogen fixation capability of endophytic clostridia by acetylene reduction and reverse transcription-PCR targeted to nifH transcript and ribosomal RNA (2006)
  • Author(s): Saito, A., K.Minamisawa
  • Journal Title: Microbes Environ. (in press)
• [Journal Article] 微生物と植物の共生相互作用の科学特集 : 微生物バイジー (2005)
  • Author(s): 南澤究
  • Journal Title: 学術月報 Vol.58, No.10 Pages: 19-24
• [Journal Article] 土壌微におけるポストゲノム研究の現状と将来 (2005)
  • Author(s): 福井学、南澤究、笠原康裕、町田雅之、早津雅仁、妹尾啓史
  • Journal Title: 日本土壌肥料学雑誌 Vol.76, No.4 Pages: 523-529
• [Journal Article] Expression of the nifH gene of a Herbaspirillum endophyte in wild rice species : daily rhythum during the light-dark cycle. (2005)
  • Author(s): You, M., Nishiguchi, T., Saito, A., Isawa, T., Mitsui, H., Minamisawa, K.
  • Journal Title: Appl.Environ.Microbiol. 71 Pages: 8183-8190
• [Journal Article] 微生物と植物の共生相互作用の科学 (2005)
  • Author(s): 南澤 究
  • Journal Title: 学術月報 58(10) Pages: 747-752
• [Presentation] 変異型dnaQプラスミドを用いたBradyrhizobium japonicum高N_2O還元酵素活性変異株の取得 (2009)
  • Author(s): 板倉学, 田畑和文, 江田志磨, 三井久幸, 村上紀里子, 安田淳一, 南澤究
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
• [Presentation] 植物生息微生物の群集構造に対する根粒共生系の影響 (2009)
  • Author(s): 南澤究、池田成志、大久保卓、金子貴一、Lynn Ester E. Rallos、江田志摩、三井久幸、佐藤修正、中村保一、田畑哲之
  • Organizer: 第3回ゲノム微生物学会
  • Place of Presentation: 東京
• [Presentation] ダイズ根粒根圏からのN_2O発生における根粒菌の寄与 (2008)
  • Author(s): 稲葉尚子、田邉謙二、菊池真和、江田志磨、南澤究
  • Organizer: 日本土壌肥料学会東北支部会宮城大会
  • Place of Presentation: 仙台
  • Year and Date: 2008-07-02
• [Presentation] 異なる根粒形成表現型ダイズにおける微生物多様性解析 (2008)
  • Author(s): 池田成志, Lynn Rallos, 大久保卓, 江田志磨, 稲葉尚子, 三井久幸, 南澤究
  • Organizer: 本微生物生態学会第24回大会
  • Place of Presentation: 札幌
• [Presentation] ダイズ根粒菌変異型daaQプラスミドを用いた高N_2O還元酵素活性変異株の取得 (2008)
  • Author(s): 板倉学, 田畑和文, 江田志磨, 三井久幸, 村上紀里子, 安田淳一, 南澤究
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 札幌
• [Presentation] 老化ダイズ根粒からのN_2O発生における根粒菌の寄与 (2008)
  • Author(s): 稲葉尚子、田邉謙二、菊池真和、江田志磨、南澤究
  • Organizer: 日本微生物生態学会第24回大会
  • Place of Presentation: 札幌
• [Presentation] ゲノム情報に基づいた植物共生細菌の環境応答と物質循環機能の解明 (2008)
  • Author(s): 南澤究
  • Organizer: 日本土壌微生物学会2008年度大会シンポジウム
  • Place of Presentation: 静岡
• [Presentation] 老化ダイズ根粒からのN 2O発生における根粒菌の寄与 (2008)
  • Author(s): 稲葉尚子
  • Organizer: 日本微生物生態学会第24回大会
  • Place of Presentation: 札幌
• [Presentation] 農業におけるゲノム情報の利用、豊橋技術科学大学・農業環境技術研究所共催公開シンポジウム「未来型農業都市社会と食農産業クラスターの構築に向けて」 (2007)
  • Author(s): 南澤究
  • Organizer: Invitedspeaker
  • Place of Presentation: 豊橋
  • Year and Date: 2007-03-12
• [Presentation] 微生物生態学における環境ゲノミクス (2007)
  • Author(s): 南澤究
  • Organizer: 第23回日本微生物生態学会シンポジウム
  • Place of Presentation: 松山
• [Presentation] ダイズ根粒根圏のN_2O発生に関わる微生物の解明 (2007)
  • Author(s): 菊池真和、田邉謙二、池田成志、江田志磨、三井久幸、南澤究
  • Organizer: 第23回微生物生態学会
  • Place of Presentation: 松山
• [Presentation] ダイズ根粒菌Bradyrhizobium japonicum2におけるN_2O還元酵素高発現株の作成 (2007)
  • Author(s): 植竹佑輔、千葉芳里、平山潤太、江田志磨、三井久幸、南澤究
  • Organizer: 第23回微生物生態学会
  • Place of Presentation: 松山
• [Presentation] ダイズ根粒根圏のN_2O発生と生物群集構造解析 (2006)
  • Author(s): 田邉謙二、池田成志、江田志磨、南澤究
  • Organizer: 日本土壌肥料学会2006年度大会
  • Place of Presentation: 秋田
• [Presentation] ダイズ根粒菌のN_2Oパラドックス (2005)
  • Author(s): 南澤究、千葉芳里、平山潤太、鮫島玲子
  • Organizer: 日本土壌微生物学会2005年度大会
  • Place of Presentation: 名古屋
• [Remarks]
  • URL: http://www.ige.tohoku.ac.jp/chiken/publication/new.html