• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Biochemical analysis of glycoproteins involved in gibberellin signal transduction in cereal seeds

Research Project

Project/Area Number 17380071
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

SUZUKI Yoshihito  The University of Tokyo, Graduate School of Agricultural Life Sciences, Associate Professor (90222067)

Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥13,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2007: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2006: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2005: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsArabinogalactan protein / aleurone laver / defense response / gibberellin / 防御応答 / ジベレリン / オオムギ / イネ / アミラーゼ / マイクロアレイ
Research Abstract

Arabinogalactan-proteins (AGPs) are a group of glycoproteins highly glycosylated with arabinogalactan polysaccharide and have been characterized by its specific binding to b-glucosyl Yariv reagent (b-G1cY). Another characteristics of AGPs is glycosylphosphatidylinositol (GPI)-anchoring, suggestive of their possible involvement in signal transduction. We have found that a-amylase induction by GA in barley aleurone protoplasts was inhibited by b-G1cY. Microarray analysis indicated that b-G1cY was largely effective in repressing both the GA-induced-and repressed-gene expressions. In addition, quite a number of defense-related genes were up-reguated by b-G1cY, including specific WRKYs and a protein kinase whose inhibitory effects on GA signaling had been shown. Plant defense signaling triggered by jasmonic acid or chitin elicitor could inhibit GA-inducible genes in aleurone cells. These results indicate that GA signaling in aleurone cells is under regulation of defense-related ding. It is also probable that AGPs are involved in the perception of stimuli causing defense responses.
Using b-G1cY, a novel early nodulin like-protein, designated as OsENODL1, was identified as a specific AGP in aleurone layers of rice. The RNAi knockdown lines of OsENODL1 and its homologous gene were prepared and the possible role of the proteins in seed formation was shown. Yeast two hybrid screening also gave a protein which interacts with early nodulin like domain. The protein was related to defense response in plants and is induced by infection, suggesting that the interaction of the protein with OsENODL1 upon infection trigger defense signaling just as b-G1cY triggered defense signaling, and inhibit GA signaling.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (18 results)

All 2008 2007 2006 2005

All Journal Article (6 results) (of which Peer Reviewed: 2 results) Presentation (12 results)

  • [Journal Article] Defense-Related Signaling by Interaction of Arabinogalactan Proteins and β-Glucosyl Yariv Reagent Inhibits Gibberellin Signaling in Barley Aleurone Cells.2008

    • Author(s)
      K. Mashiguchi
    • Journal Title

      Plant Cell Phyisology 49

      Pages: 178-190

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Defense-Related Signaling by Interaction of Arabinogalactan Proteins and β-Glucosyl Yariv Reagent Inhibits Gibberellin Signaling in Barley Aleurone Cells2008

    • Author(s)
      K. Mashiguchi
    • Journal Title

      Plant Cell Phyisology 49

      Pages: 178-190

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Defense-Related Signaling by Interaction of Arabinogalactan Proteins and b-Glucosyl Yariv Reagent Inhibits Gibberellin Signaling in Barley Aleurone Cells2008

    • Author(s)
      K.Mashiguchi, E.Urakami, M.Hasegawa, K.Sanmiya, I.Matsumoto, L.Yamauchi, T.Asam 1 and Y.Suzuki
    • Journal Title

      Plant Cell Physiol 49

      Pages: 178-190

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高等植物におけるアラビノガラクタン蛋白質の構造と機能2006

    • Author(s)
      鈴木 義人
    • Journal Title

      化学と生物 44・3

      Pages: 178-184

    • NAID

      10017248219

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 高等植物におけるアラビノガラクタン蛋白質の構造と機能について2005

    • Author(s)
      鈴木義人
    • Journal Title

      化学と生物 44

      Pages: 178-184

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Structure and functions of arabinogalactan proteins in higher plants2005

    • Author(s)
      Yoshihito Suzuki
    • Journal Title

      Kagaku to Seibut 44

      Pages: 178-184

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ヤリブ試薬による大麦糊粉層ジベレリン情報伝達の阻害に関する研究2007

    • Author(s)
      増口潔
    • Organizer
      植物化学調節学会
    • Place of Presentation
      静岡
    • Year and Date
      2007-10-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Study on inhibitory mechanism of Yariv reagent on gibberellin signal transduction in barley aleurone layers2007

    • Author(s)
      K. Mashiguch
    • Organizer
      The Japanese Soc. for Chemical Regulation of Plants
    • Year and Date
      2007-10-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Defense-Related Signaling by Interaction of Arabinogalactan Proteins and β-Glucosyl Yariv Reagent Inhibits Gibberellin Signaling in Barley Aleurone Cells.2007

    • Author(s)
      K. Mashiguchi
    • Organizer
      19th Annual Meeting of International Plant Growth Substances Association
    • Place of Presentation
      Puerto Vallarta, Mexico
    • Year and Date
      2007-07-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Defense-Related Signaling by Interaction of Arabinogalactan Proteins and β-Glucosyl Yariv Reagent Inhibits Gibberellin Signaling in Barley Aleurone Cell2007

    • Author(s)
      K. Mashiguchi
    • Organizer
      19th Annual Meeting of International Plant Growth Substances Associatio
    • Place of Presentation
      Puerto Vallarta, Mexico
    • Year and Date
      2007-07-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 穀類種子糊粉層におけるアラビノガラクタン蛋白質群の機能解析2007

    • Author(s)
      増口潔
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Functional Analysis of arabinogalactan proteins in aleurone layers of cereal seeds2007

    • Author(s)
      K. Mashiguchi
    • Organizer
      Japan Society for Biosci., Biotechnol., and Agrochem
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マイクロアレイを用いたオオムギ糊粉層におけるアラビノガラクタン蛋白質機能の解析2006

    • Author(s)
      浦上恵理子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都
    • Year and Date
      2006-03-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Functional analysis of arabinogalactan proteins in barley aleurone layers using microarray analysis2006

    • Author(s)
      E. Urakam
    • Organizer
      Japan Society for Biosci., Biotechnol., and Agrochem
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-03-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Isolation and identification of arabinogalactan proteins and novel β-Glucosyl Yariv-reactive proteins from seeds of rice.2005

    • Author(s)
      K. Mashiguchi
    • Organizer
      The 2005 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2005-12-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Isolation and identification of arabinogalactan proteins and novel β-Glucosyl Yariv-reactive proteins from seeds of rice2005

    • Author(s)
      K. Mashiguchi
    • Organizer
      The 2005 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2005-12-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Glycoproteins having arabinogalactan carbohydrate chains for plant growth.2005

    • Author(s)
      Y. Suzuki
    • Organizer
      Xth France-Japan Workshop on Plant Sciences
    • Place of Presentation
      Toulouse, France
    • Year and Date
      2005-09-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Glycoproteins having arabinogalactan carbohydrate chains for plant growth2005

    • Author(s)
      Y. Suzuki
    • Organizer
      Xth France-Japan Workshop on Plant Sciences
    • Place of Presentation
      Toulouse, France
    • Year and Date
      2005-09-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi