| Project/Area Number |
17380079
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Hiroshima University |
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Principal Investigator |
KATO Norihisa Hiroshima University, Graduate School of Biosphere Science, Professor (20144892)
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| Co-Investigator(Kenkyū-buntansha) |
YANAKA Noriyuki Hiroshima University, Graduate School of Biosphere Science, Associate Professor (70346526)
MATSUBARA Kiminori Hiroshima University, Graduate School of Erboaton, Associate Professor (90254565)
OKA Tatsuzo Kagoshima University, Faculty of Agriculture, Professor (50116795)
MIZUSHINA Yoshiyuki Kobe-Gakuin University, Departrnent of Nutritional Science, Associate Professor (20307705)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥12,320,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2007: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2006: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | Vitamin B6 / ageing / cancer / NFkB / autophagy / DNA microarray / oxidative stress / obese / 大腸癌 / ホモシステイン / 銅イオン / PPARγ / IMP脱水素酵素 / 糖尿病 / 皮膚がん / 乳がん / SAMPマウス / 炎症 / 脳疾患 |
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| Research Abstract |
Recently, vitamin B6 (B6) has been reported to prevent several diseases. This study was performed to examine the functions of 136 as an anti-ageing factor. Recent studies elucidate the important roles of NFkB-dependent inflammation both in the development of several diseases and in the ageing. Thus, we found the inhibitory effect of B6 on the NFkB activation in LPS-stimulated macrophages. We next examined if anti-tumor effect of dietary B6 can be observed in the development of mammary and skin cancer. The results indicated that consumption of B6 suppressed mammary carcinogenesis in DMBA-treated rats, although, unexpectedly, consumption of B6 elevated skin carcinogenesis in UVB-treated hairless mice. Recently, autophagy has been suggested to be involved in the progression of cancers. In this study, we discovered the inhibitory effect of 86 on autophagy, implying the involvement of lower autophagy in the mechanisms of the anti-tumor effect of 86 in the colon and mammary cancers. To elucidate the molecular mechanisms of anti-tumor effect of B6, DNA microarray analysis was conducted for the comprehensive screening of colon factors which response to dietary B6. As a result, several genes were obtained to response to B6. During study on the mechanisms of anti-oxidant function of B6, we found that the presence of copper ion greatly stimulates the anti-oxidant function of B6, implying the copper-dependent anti-oxidant mechanisms of B6. Finally, to study the interaction of B6 and metabolic syndrome, we investigated the status of B6 metabolism in obese-diabetic mice. The results showed the characteristic alteration of B6 metabolites especially in serum and liver of the obese mice.
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