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Molecular ecological studies on ectomycorrhizal symbiotic system in pine forests

Research Project

Project/Area Number 17380089
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学・森林工学
Research InstitutionThe University of Tokyo

Principal Investigator

MATSUSHITA Norihisa  The University of Tokyo, Graduate School of Agricultural & Life Science, Lecturer (00282567)

Co-Investigator(Kenkyū-buntansha) FUKUDA Kenji  The University of Tokyo, Graduate school of Frontier Scienses, Professor (30208954)
SUZUKI Kazuo  Nihon University, Faculty of Bioresource Sciences, Professor (80162931)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥15,710,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2006: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2005: ¥6,900,000 (Direct Cost: ¥6,900,000)
Keywordsectomycorrhiza / pine forest / symbiosis / molecular ecology / microsatellite marker / spore germination / 外生菌根菌 / 菌類 / マツ科樹木 / ライゾトロン / 海岸林 / 根圏微生物 / 細菌 / マツタケ
Research Abstract

1. Nine simple sequence repeat (SSR) loci were isolated from an ectomycorrhizal fungus Suillus bovinus by dual-suppression PCR. One of these was confirmed to be species specific and codominant, suggesting applicability for the analysis of belowground population structure and gene flow of S. bovinus.
2. Spore of S. bovinus germinated only by the combination of activated charcoal treatment of media and co-culture with seedlings of Pinus densiflora. Abietic acid, which has been reported to be a genus-specific activator of germination for Suillus species, showed no effect, suggesting some other substances contained in root exudates induced germination of spores of S. bovinus.
3. We investigated the effects of flavonoids on basidiospore germination of S. bovinus by the diffusion gradient assay on water agar plates. Seven out of the 11 flavonoids tested had greater effects than controls. The results suggest that flavonoids play a role as signaling molecules in symbiotic relationships between woody plants and ectomycorrhizal fungi.
4. Results from the sequence data of the rDNA-ITS region suggest that strains of Tricholoma matsutake in broad-leaved forests are genetically the same species as those in coniferous forests.
5. The composition of ectomycorrhizal communities on mature trees and ectomycorrhizal propagule banks in soils showed a successional gradient along the secondary vegetation gradient, but many common morphotypes were present among the different vegetation stages.
6. Results from the survey of ectomycorrhizal fungal fruit bodies in the damaged pine forest caused by pine wilt disease suggest that the diversity of ectomycorrhizal fungi reduces with heavy damage to pines and that the other ectomycorrhizal tree species can provide refugia for ectomycorrhizal fungi when pine trees are damaged.
7. We demonstrate that the roots of mature pine trees can be successfully inoculated with a symbiotic ectomycorrhizal fungus, the valuable matsutake mushroom.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (50 results)

All 2008 2007 2006 2005

All Journal Article (36 results) (of which Peer Reviewed: 17 results) Presentation (14 results)

  • [Journal Article] Effect of aerial spraying of insecticide as a control measure of pine wilt disease.2008

    • Author(s)
      Ugawa, S.
    • Journal Title

      Forest Pathology 38

      Pages: 16-28

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of aerial spraying of insecticide as a control measure of pine wilt disease2008

    • Author(s)
      Ugawa, S.
    • Journal Title

      Forest Pathology 38

      Pages: 16-28

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Development of SSR markers from an ectomycorrhizal fungus Suillus bovinus.2007

    • Author(s)
      Kikuchi, K.
    • Journal Title

      Mycoscience 48

      Pages: 255-258

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Discrimination of Tricholoma species by species-specific ITS primers.2007

    • Author(s)
      Kikuchi, K.
    • Journal Title

      Mycoscience 48

      Pages: 316-320

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Flavonoids induce germination of basidiospores of the ectomycorrhizal fungus Suillus bovinus.2007

    • Author(s)
      Kikuchi, K.
    • Journal Title

      Mycorrhiza 17

      Pages: 563-570

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] ライゾトロンによる樹木根系の観察法.2007

    • Author(s)
      松下範久
    • Journal Title

      樹木医学研究 11

      Pages: 121-124

    • NAID

      110007862609

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Changes in fungal communities in evergreen broad-leaved forests across agradient of urban to rural areas in Japan.2007

    • Author(s)
      Ochimaru, T.
    • Journal Title

      Canadian Journal of Forest Research 37

      Pages: 247-258

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Coexistence of Abies mariesii and A. veitchii in a subalpine fir-wave forest.2007

    • Author(s)
      Ugawa, S.
    • Journal Title

      Canadian Journal of Forest Research 37

      Pages: 2142-2152

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Ectomycorrhizal community on tree roots and in soil propagule banks along a secondary successional vegetation gradient.2007

    • Author(s)
      Yamashita, S.
    • Journal Title

      Forest Science 53

      Pages: 635-644

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Development of SSR markers from an ectomycorrhizal fungus Suillus hovinus2007

    • Author(s)
      Kikuchi, K.
    • Journal Title

      Mycoscience 48

      Pages: 255-258

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Discrimination of Tricholoma species by species-specific ITS primers2007

    • Author(s)
      Kikuchi, K.
    • Journal Title

      Mycoscience 48

      Pages: 316-320

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Flavonoids induce germination of basidiospores of the ectomycorrhizal fungus Suillus bovinus2007

    • Author(s)
      Kikuchi, K.
    • Journal Title

      Mycorrhiza 17

      Pages: 563-570

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Changes in fungal communities in evergreen broad-leaved forests across a gradient of urban to rural areas in Japan2007

    • Author(s)
      Ochimaru, T.
    • Journal Title

      Canadian Journal of Forest Research 37

      Pages: 247-258

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Coexistence of Abies mariesii and A. veitchii in a subalpine fir-wave forest2007

    • Author(s)
      Ugawa, S.
    • Journal Title

      Canadian Journal of Forest Research 37

      Pages: 2142-2152

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Ectomycorrhizal community on tree roots and in soil propagule banks along a secondary successional vegetation gradient2007

    • Author(s)
      Yamashita, S.
    • Journal Title

      Forest Science 53

      Pages: 635-644

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Flavonoids induce germination of basidiospore of the ectomycorrhizal fungus Suillus bovinus2007

    • Author(s)
      Kikuchi K
    • Journal Title

      Mycorrhiza 17

      Pages: 563-570

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of SSR markers from an ectomycorrhizal fungus Suillus bovinus2007

    • Author(s)
      Kikuchi K
    • Journal Title

      Mycoscience 48

      Pages: 255-258

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discrimination of Tricholoma species by species-specific ITS primers2007

    • Author(s)
      Kikuchi K
    • Journal Title

      Mycoscience 48

      Pages: 316-320

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ライゾトロンによる樹木根系の観察法2007

    • Author(s)
      松下 範久
    • Journal Title

      樹木医学研究 11

      Pages: 121-124

    • NAID

      110007862609

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 九十九里浜クロマツ海岸林における地下部外生菌根菌群集の構造と経時変化2007

    • Author(s)
      近藤那海子
    • Journal Title

      第118回日本森林学会大会学術講演集

    • Related Report
      2006 Annual Research Report
  • [Journal Article] アミタケ胞子発芽に及ぼすフラボノイドの効果2007

    • Author(s)
      菊池研介
    • Journal Title

      第118回日本森林学会大会学術講演集

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 界面活性剤および植物油が外生菌根菌の菌糸成長に及ぼす影響2007

    • Author(s)
      進藤克実
    • Journal Title

      第118回日本森林学会大会学術講演集

      Pages: 3143-3143

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 人工条件下におけるアミタケ胞子の発芽法の検討2006

    • Author(s)
      菊池研介
    • Journal Title

      日本菌学会会報 47

      Pages: 37-40

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 隠れた森の主役「菌根」2006

    • Author(s)
      松下範久
    • Journal Title

      森林技術 770

      Pages: 25-25

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] マツタケの人工栽培に挑戦2006

    • Author(s)
      松下範久
    • Journal Title

      弥生 43

      Pages: 4-4

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] マツタケ栽培の動向2006

    • Author(s)
      鈴木和夫
    • Journal Title

      菌蕈 2006(12)

      Pages: 12-15

    • NAID

      40015201573

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] IKONOSデ-タを利用したマツ枯れ被害林分の抽出2006

    • Author(s)
      龍原 哲
    • Journal Title

      森林計画学会誌 40

      Pages: 203-210

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Successful inoculation of mature pine with Tricholoma matsutake.2005

    • Author(s)
      Guerin-Laguette, A.
    • Journal Title

      Mycorrhiza 15

      Pages: 301-305

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Genetic relationship of Tricholoma matsutake and T. nauseosum from the northern hemisphere based on analyses of ribosomal DNA spacer regions.2005

    • Author(s)
      Matsushita, N.
    • Journal Title

      Mycoscience 46

      Pages: 90-96

    • NAID

      10015667022

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The response of ectomycorrhizal fungi on Pinus densiflora seedling roots to liquid culture2005

    • Author(s)
      Ugawa, S.
    • Journal Title

      Journal of Forest Research 10

      Pages: 233-237

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Successful inoculation of mature pine with Tricholoma matsutak2005

    • Author(s)
      Guerin-Laguette, A.
    • Journal Title

      Mycorrhiza 15

      Pages: 301-305

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Genetic relationship of Tricholoma matsutake and T nauseosum from the northern hemisphere based on analyses of ribosomal DNA spacer regions2005

    • Author(s)
      Matsushita, N.
    • Journal Title

      Mycoscience 46

      Pages: 90-96

    • NAID

      10015667022

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The response of ectomycorrhizal fungi on Pin us d''ensitlora seedling roots to liquid culture2005

    • Author(s)
      Ugawa, S.
    • Journal Title

      Journal of Forest Research 10

      Pages: 233-237

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification of Armillaria species in Japan using PCR-RFLP analysis of rDNA intergenic spacer region and comparisons of Armillaria species in the world2005

    • Author(s)
      Matsushita, N.
    • Journal Title

      Journal of Forest Research 10

    • NAID

      10016447298

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetic relationship of Tricholoma matsutake and T.nauseosum from the northern hemisphere based on analyses of ribosomal DNA spacer regions2005

    • Author(s)
      Matsushita, N.
    • Journal Title

      Mycoscience 46

      Pages: 90-96

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Successful inoculation of mature pine with Tricholoma matsutake2005

    • Author(s)
      Guerin-Laguette, A.
    • Journal Title

      Mycorrhiza 15

      Pages: 301-305

    • Related Report
      2005 Annual Research Report
  • [Presentation] 外生菌根菌性子実体直下における群集構造の経時変化2008

    • Author(s)
      近藤那海子
    • Organizer
      第119回日本森林学会大会
    • Place of Presentation
      東京農工大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 富士山火山荒原における外生菌根菌ハマニセショウロのジェネット変動2008

    • Author(s)
      丸山 永
    • Organizer
      第119回日本森林学会大会
    • Place of Presentation
      東京農工大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 中国雲南省産のマツタケとブナ科樹木との菌根共生2008

    • Author(s)
      進藤克実
    • Organizer
      第119回日本森林学会大会
    • Place of Presentation
      東京農工大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アミタケの胞子発芽に及ぼすフラボノイドの効果2007

    • Author(s)
      菊池研介
    • Organizer
      第118回日本森林学会大会
    • Place of Presentation
      九州大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] クロマツ海岸林における外生菌根菌群集の種組成と空間構造2007

    • Author(s)
      近藤那海子
    • Organizer
      第118回日本森林学会大会
    • Place of Presentation
      九州大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 界面活性剤および植物油が外生菌根菌の菌糸成長に及ぼす影響2007

    • Author(s)
      進藤克実
    • Organizer
      第118回日本森林学会大会
    • Place of Presentation
      九州大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Reduction of diversity in ectomycorrhizal fungal flora caused by the damaged from pine wilt disease2006

    • Author(s)
      Ugawa, S.
    • Organizer
      IUFRO UNIT 7.03.12 Inaugural Meeting
    • Place of Presentation
      Jedlnia, Poland
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of aerial spraying of insecticide as the control measure of pine wilt disease.2006

    • Author(s)
      Ugawa, S.
    • Organizer
      International Symposium Pine wilt: a worldwide threat to forest ecosystems
    • Place of Presentation
      Lisbon, Portugal
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アミタケの胞子接種により形成された菌根の遺伝子型と核相.2006

    • Author(s)
      菊池研介
    • Organizer
      第117回日本森林学会大会
    • Place of Presentation
      東京農業大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 九十九里浜海岸クロマツ林における外生菌根菌群集構造2006

    • Author(s)
      近藤那海子
    • Organizer
      第117回日本森林学会大会
    • Place of Presentation
      東京農業大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 海岸林におけるニセアカシアを宿主とした根粒菌の多様性2006

    • Author(s)
      白石彩水
    • Organizer
      第117回日本森林学会大会
    • Place of Presentation
      東京農業大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of aerial spraying of insecticide as the control measure of pine wilt disease2006

    • Author(s)
      Ugawa, S.
    • Organizer
      International Symposium Pine wilt : a worldwide threat to forest ecosystems
    • Place of Presentation
      Lisbon, Portugal
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genotyping of mycorrhizas synthesized by spore inoculation of Suillus bovinus2005

    • Author(s)
      Kikuchi, K.
    • Organizer
      The Mycological Society of America/The Mycological Society of Japan Joint Meeting
    • Place of Presentation
      Hilo, Hawaii
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Fungal flora of evergreen broad-leaved forest and its change caused by urbanization2005

    • Author(s)
      Ochimaru, T.
    • Organizer
      XXII IUFRO World Congress
    • Place of Presentation
      Brisbane, Queensland
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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