Research of tree pollen movement based on genotyping of single pollen grains

• Project/Area Number: 17380095
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 林学・森林工学
• Research Institution: Kyoto University (2006-2007); Hiroshima University (2005)
• Principal Investigator: ISAGI Yuji Kyoto University, Graduate SrhmlofAgricultme, Professor (50325130)
• Co-Investigator(Kenkyū-buntansha): SUYAMA Yoshihisa Tohoku University, Graduate School of Agriculture, Associate Professor (60282315) ; SHIBATA Mitsue Forestry and Foipa Products Research Institute, Tohoku Branch, Head of Research Team (10343807)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥12,910,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥1,110,000); Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2006: ¥3,900,000 (Direct Cost: ¥3,900,000); Fiscal Year 2005: ¥4,200,000 (Direct Cost: ¥4,200,000)
• Keywords: pollination / genetic diversity / reproductive ecology / 適応度 / 繁殖成功 / 生物保全 / 被子植物

Research Abstract

The pollination process of tree species are important for the maintenance and regeneration of tree communities, and the genetic traits of pollen grains that are transported to flowers influence the reproduction and fitness of plants. Despite the importance of insect-pollination systems, understanding of those systems is still larking due to the absence of the genetic analysis of pollen grains. We extracted haploid nuclear DNA from a single pollen grain by using a simple pollen DNA extraction method. Multiple microsatellite genotypes of a single pollen, which are useful for ecological studies, e. g. pollen dispersal pattern, paternity analysis and evaluation of pollinators, were determined simultaneously within a single reaction tube. Using nine pairs of microsatellite primers, more than 90% of genotypes were successfully determined. Using the method developed for the present research, we evaluated the pollination efficiencies of bumblebees, flower beetles that visited the flowers of Magnolia obovata. Most of the pollen adhering to bumblebees was self-pollen. This result suggests that visitation by the insects may cause geitonogamous pollen flow and negatively affect the reproduction of M. obovata, causing inbreeding depression. In contrast, flower beetles transported large amounts of genetically diverse outcross pollen. The present results suggest that certain beetle species contribute to the pollination of M. obovata quantitatively and quantitatively. Direct gentic analysis of pollen grains will advance the understanding of plant mating systems, and may shed light on the mutualism and co-evolution of trees and flower visitors.

Research Products

• [Journal Article] Development of polymorphic microsatellite markers in Acer mono Maxim. (2008)
  • Author(s): Kikuchi, S. and Shibata, M.
  • Journal Title: Molecular Ecology Notes 8 Pages: 339-341
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Development of microsatellite markers for Echinops setifer (Asteraceae), an endangered grassland plant species in Japan (2008)
  • Author(s): Kaneko, S., Isagi, Y., Nakagoshi, N
  • Journal Title: Conservation Genetic 8 Pages: 1231-1233
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development of polymorphic microsatellite markers in Acer mono Maxim (2008)
  • Author(s): Kikuchi, S., Shibata, M.
  • Journal Title: Molecular Ecology Notes 8 Pages: 339-341
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development of polymorphic microsatellite markers in Acer mono Maxim. (2008)
  • Author(s): Kikuchi S, Shibata M
  • Journal Title: Molecular Ecology Resources (印刷中)
  • Peer Reviewed
• [Journal Article] Development of microsatellite markers for Bambusa arnhemica (Poaceae: Bambuseae), a bamboo endemic to northern Australia. (2008)
  • Author(s): Kaneko S, Franklin DC, Yamasaki N, Isagi Y
  • Journal Title: Conservation Genetics (印刷中)
  • Peer Reviewed
• [Journal Article] Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered relict plant on the Ryukyu Islands and Taiwan. (2008)
  • Author(s): Dan T, Mitsui Y, Ikeda H, Isagi Y, Setoguchi H
  • Journal Title: Conservation Genetics (accepted)
  • Peer Reviewed
• [Journal Article] Genetic differentiation among populations in an oceanic island: the case of Metrosideros boninesis, an endangered endemic tree species in the Bonin Islands. (2008)
  • Author(s): Kaneko S, Isagi Y, Nobushima F
  • Journal Title: Conservation Genetics (accepted)
  • Peer Reviewed
• [Journal Article] Genetic and reproductive consequences of forest fragmentation for populations of Magnolia obovata. (2007)
  • Author(s): Isagi, Y., Tateno, R., Matsuki, Y., Hirao, A., Watanabe, S., and Shibata, M.
  • Journal Title: Ecological Research 22 Pages: 382-389
  • NAID: 10019932770
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effective pollen dispersal is enhanced by the genetic structure of an Aesculus turbinata population. (2007)
  • Author(s): Isagi, Y., Saito, D., Kawaguchi H., Tateno, R., and Watanabe S.
  • Journal Title: Journal of Ecology 95 Pages: 983-990
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 森林で花粉はどの様に動いているか (2007)
  • Author(s): 井鷺裕司
  • Journal Title: 森林科学 50 Pages: 42-45
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Development of microsatellite markers for Echinops setifer(Asteraceae), an endangered grassland plant species in Japan. (2007)
  • Author(s): Kaneko, S., Isagi, Y., and Nakagoshi, N
  • Journal Title: Conservation Genetics 8 Pages: 1231-1233
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Development of microsatellite markers for Metrosideros boninensis(Myrtaceae), an endangered endemic plant species from the Bonin Islands, Japan. (2007)
  • Author(s): Kaneko, S., Isagi, Y., and Nobushima, F.
  • Journal Title: Conservation Genetics 8 Pages: 753-755
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Isolation and characterization of microsatellite loci in Melicope quadrilocularis(Rutaceae), an endemic plant species of the Bonin Islands, Japan, and cross-species amplification in closely related taxa. (2007)
  • Author(s): Katoh, S., Kaneko, S., Isagi, Y., Murakami, N., and Kato, H.
  • Journal Title: Conservation Genetics 8 Pages: 1487-1490
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The determination of multiple microsatellite genotypes and DNA sequences from a single pollen grain. (2007)
  • Author(s): Matsuki, Y., Isagi, Y., and Suyama, Y.
  • Journal Title: Molecular Ecology Notes 7 Pages: 194-198
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Genetic and reproductive consequences of forest fragmentation for populations of Magnolia obovata (2007)
  • Author(s): Isagi, Y., Tateno, R., Matsuki, Y., Hirao, A., Watanabe, S., Shibata, M
  • Journal Title: Ecological Research 22 Pages: 382-389
  • NAID: 10019932770
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effective pollen dispersal is enhanced by the genetic structure of an Aesculus turbinata population (2007)
  • Author(s): Isagi, Y., Saito, D., Kawaguchi, H., Tateno, R., Watanabe, S
  • Journal Title: Journal of Ecology 95 Pages: 983-990
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] How pollen grains move in forest ecosystems (2007)
  • Author(s): Isagi, Y
  • Journal Title: Forest Science 50 Pages: 42-45
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development of microsatellite markers for Metrosideros boninensis (Myrtaceae), an endangered endemic plant species from the Bonin Islands, Japan (2007)
  • Author(s): Kaneko, S., Isagi, Y., Nobushima, F
  • Journal Title: Conservation Genetics 8 Pages: 753-755
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isolation and characterization of microsatellite loci in Melicope quadrilocularis (Rutaceae), an endemic plant species of the Bonin Islands, Japan, and cross-species amplification in closely related taxa (2007)
  • Author(s): Katoh, S., Kaneko, S., Isagi, Y., Murakami, N., Kato, H
  • Journal Title: Conservation Genetics 8 Pages: 1487-1490
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The determination of multiple microsatellite genotypes and DNA sequences from a single pollen grain (2007)
  • Author(s): Matsuki, Y., Isagi, Y., Suyama, Y
  • Journal Title: Molecular Ecology Notes 7 Pages: 194-198
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genetic and reproductive consequences of forest fragmentation for populations of Magnolia obovata. (2007)
  • Author(s): Isagi Y, Tateno R, Matsuki Y, Hirao A, Watanabe S, Shibata M
  • Journal Title: Ecological Research 22 Pages: 382-389
  • NAID: 10019932770
  • Peer Reviewed
• [Journal Article] Development of microsatellite markers for Metrosideros boninensis (Myrtaceae), an endangered endemic plant (2007)
  • Author(s): Kaneko S, Isagi Y, Nobushima F
  • Journal Title: Conservation Genetics 8 Pages: 753-755
  • Peer Reviewed
• [Journal Article] Development of microsatellite markers for Echinops setifer (Asteraceae), an endangered grassland plant (2007)
  • Author(s): Kaneko S, Isagi Y, Nakagoshi N
  • Journal Title: Conservation Genetics 8 Pages: 1231-1233
  • Peer Reviewed
• [Journal Article] Isolation and characterization of microsatellite loci in Melicope quadrilocularis (Rutaceae), an endemic plant species of the Bonin Islands Japan, and cross-species amplification in closely related taxa. (2007)
  • Author(s): Katoh S, Kaneko S, Isagi Y, Murakami N, Kato H
  • Journal Title: Conservation Genetics 8 Pages: 1487-1490
  • Peer Reviewed
• [Journal Article] Effective pollen dispersal is enhanced by the genetic structure of an Aesculus turbinata population. (2007)
  • Author(s): Isagi Y, Saito D, Kawaguchi H, Tateno R, Watanabe S
  • Journal Title: Journal of Ecology 95 Pages: 983-990
  • Peer Reviewed
• [Journal Article] 森林で花粉はどの様に動いているか (2007)
  • Author(s): 井鷺 裕司
  • Journal Title: 森林科学 50 Pages: 42-45
• [Journal Article] The determination of multiple microsatellite genotypes and DNA sequences from a single pollen grain. (2007)
  • Author(s): Matsuki Y, Isagi Y, Suyama Y
  • Journal Title: Molecular Ecology Notes 7 Pages: 194-198
• [Journal Article] Microsatellite loci for a sparsely distributed species, Kalopanax pictus(Araliaceae)in Japanese temperate forests. (2006)
  • Author(s): Fujimori, N., Kenta, T., Kondo, T., Kameyama, Y., Isagi, Y., and Nakashizuka, T.
  • Journal Title: Molecular Ecology Notes 6 Pages: 468-469
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Reproductive success and distance to conspecific adults in the sparsely distributed tree Kalopanax pictus. (2006)
  • Author(s): Fujimori, N., Samejima, H., Kenta, T., Ichie, T., Shibata, M., Iida, S., and Nakashizuka, T.
  • Journal Title: Journal of Plant Research 119 Pages: 195-203
  • NAID: 10019164674
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Seasonal changes in pollinator activity influence the pollen dispersal and seed production of the alpine shrub Rhododendron aureum(Ericaceae). (2006)
  • Author(s): Hirao, A. S., Kameyama, Y., Ohara, M., Isagi, Y., and Kudo G.
  • Journal Title: Molecular Ecology 15 Pages: 1165-1173
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effect of gene flow on spatial genetic structure in the riparian canopy tree Cercidiphyllum japonicum revealed by microsatellite analysis. (2006)
  • Author(s): Sato, T., Isagi, Y., Sakio, H., Osumi, K., and Goto, S.
  • Journal Title: Heredity 96 Pages: 79-84
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Microsatellite loci for a sparsely distributed species, Kalopanax pictus (Araliaceae) in Japanese temperate forests (2006)
  • Author(s): Fujimori, N., Kenta, T., Kondo, T., Kameyama, Y., Isagi, Y., Nakashizuka, T
  • Journal Title: Molecular Ecology Notes 6 Pages: 468-469
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Reproductive success and distance to conspecific adults in the sparsely distributed tree Kalopanax pictus (2006)
  • Author(s): Fujimori, N., Samejima, H., Kenta, T., Ichie, T., Shibata, M., lida, S., Nakashizuka, T
  • Journal Title: Journal of Plant Research 119 Pages: 195-203
  • NAID: 10019164674
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Seasonal changes in pollinator activity influence the pollen dispersal and seed production of the alpine shrub Rhododendron aureum (Ericaceae) (2006)
  • Author(s): Hirao, AS., Kameyama, Y., Ohara, M., Isagi, Y., Kudo, G
  • Journal Title: Molecular Ecology 15 Pages: 1165-1173
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of gene flow on spatial genetic structure in the riparian canopy tree Cercidiphyllum japonicum revealed by microsatellite analysis (2006)
  • Author(s): Sato, T., Isagi, Y., Sakio, H., Osumi, K., Goto S
  • Journal Title: Heredity 96 Pages: 79-84
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Microsatellite loci for a sparsely distributed species, Kalopanax pictus (Araliaceae) in Japanese temperate (2006)
  • Author(s): Fujimori N, Kenta T, Kondo T, Kameyama Y, Isagi Y, Nakashizuka
  • Journal Title: Molecular Ecology Notes 6 Pages: 468-469
• [Journal Article] Seasonal changes in pollinator activity influence pollen dispersal and seed production in the alpine shrub Rhododendron aureum (Ericaceae). (2006)
  • Author(s): Hirao AS, Kameyama Y, Ohara M, Isagi Y, Kudo G
  • Journal Title: Molecular Ecology 15 Pages: 1165-1173
• [Journal Article] Effect of gene flow on spatial genetic structure in the riparian canopy tree Cercidiphyllum japonicum revealed by microsatellite analysis. (2006)
  • Author(s): Sato T
  • Journal Title: Heredity 96 Pages: 79-84
• [Journal Article] Polymorphic microsatellite DNA markers for a relict angiosperm Cercidiphyllum japonicum Sieb. et Zucc. and their utility for Cercidiphyllum magnificum. (2005)
  • Author(s): Isagi, Y., Kudo, M., Osumi, K., Sato, T., and Sakio, H.
  • Journal Title: Molecular Ecology Notes 5 Pages: 596-598
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Ancient DNA from pollen: a genetic record of population history in Scots pine. (2005)
  • Author(s): Parducci, L., Suyama, Y., Lascoux M., and Bennett, K. D.
  • Journal Title: Molecular Ecology 14 Pages: 2873-2882
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 森林の広域・長期的な試験地から得られる成果と生き残りのための条件. (2005)
  • Author(s): 正木隆, 柴田銃江
  • Journal Title: 日本生態学会誌 55 Pages: 359-369
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Polymorphic microsatellite DNA markers for a relict angiosperm Cercidiphyllum japonicum Sieb. et Zucc. and their utility for Cercidiphyllum magnificum (2005)
  • Author(s): Isagi, Y., Kudo, M., Osumi, K., Sato, T., Sakio, H
  • Journal Title: Molecular Ecology Notes 5 Pages: 596-598
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ancient DNA from pollen : a genetic record of population history in Scots pine (2005)
  • Author(s): Parducci, L., Suyama, Y., Lascoux, M., Bennett, KD
  • Journal Title: Molecular Ecology 14 Pages: 2873-2882
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Examples of long-term and large-scale monitoring of forests : outcomes and required conditions to continue (2005)
  • Author(s): Masaki, T., Shibata, M
  • Journal Title: Japanese Journal of Ecology 55 Pages: 259-269
  • NAID: 110004746527
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Polymorphic microsatellite DNA markers for a relict angiosperm Cercidiphyllum japonicum Sieb. et Zucc and their utility for C. magnificum. (2005)
  • Author(s): Isagi Y
  • Journal Title: Molecular Ecology Notes 5 Pages: 596-598
• [Journal Article] Isolation and characterization of microsatellite loci in Shortia rotundifolia(Diapensiaceae),an endangered relict plant on the Ryukyu Islands and Taiwan.
  • Author(s): Dan, T., Mitsui, Y., Ikeda, H., Isagi, Y., and Setoguchi, H.
  • Journal Title: Conservation Genetics (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effects of population density, sex morph, and tree size on reproduction in a heterodichogamous maple, Acer mono, in a temperate forest of Japan.
  • Author(s): Shibata, M., Kikuchi S., Tanaka, H., Suweyoshi M., Yoshimaru H., and Niiyama K.
  • Journal Title: Ecological Research (In press)
  • NAID: 10025354528
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Genetic differentiation among populations in an oceanic island: the case of Metrosideros boniensis, an endangered endemic tree species in the Bonin Islands.
  • Author(s): Kaneko, S., Isagi, Y., and Nobushima, F.
  • Journal Title: Plant Species Biology (Accepted)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Pollination efficiencies of flower-visiting insects as determined by direct genetic analysis of pollen origin.
  • Author(s): Matsuki, Y., Tateno, R., Shibata, M., and Isagi, Y.
  • Journal Title: American Journal of Botany (Accepted)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered elict plant on the Ryukyu Islands and Taiwan
  • Author(s): Dan, T., Mitsui, Y., Ikeda, H., Isagi, Y., Setoguchi, H
  • Journal Title: Conservation Genetics (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of population density, sex morph, and tree size on reproduction in a heterodichogamous maple, Acer mono, in a temperate forest of Japan
  • Author(s): Shibata, M., Kikuchi, S., Tanaka, H., Suweyoshi, M., Yoshimaru, H., Niiyama, K
  • Journal Title: Ecological Research (in press)
  • NAID: 10025354528
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genetic differentiation among populations in an oceanic island : the case of Metrosideros boniensis, an endangered endemic tree species in the Bonin Islands
  • Author(s): Kaneko, S., Isagi, Y., Nobushima, F
  • Journal Title: Plant Species Biology (accepted)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Pollination efficiencies of flower-visiting insects as determined by direct genetic analysis of pollen origin
  • Author(s): Matsuki, Y., Tateno, R., Shibata, M., Isagi, Y
  • Journal Title: American Journal of Botany (accepted)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development of microsatellite markers for Metrosideros boninensis (Myrtaceae), an endangered endemic plant species from the Bonin Islands, Japan.
  • Author(s): Kaneko S, Isagi Y, Nobushima F
  • Journal Title: Conservation Genetics (in press)
• [Journal Article] Genetic and reproductive consequences of forest fragmentation for populations of Magnolia obovata.
  • Author(s): Isagi Y, Tateno R, Matsuki Y, Hirao A, Watanabe S, Shibata M
  • Journal Title: Ecological Research (in press)
  • NAID: 10019932770
• [Journal Article] Development of microsatellite markers for Echinops setifer (Asteraceae), an endangered grassland plant species in Japan.
  • Author(s): Kaneko S, Isagi Y, Nakagoshi N
  • Journal Title: Conservatin Genetics (in press)
• [Journal Article] Isolation and characterization of microsatellite loci in Melicope quadrilocularis (Rutaceae), an endemic plant species of the Bonin Islands Japan, and cross-species amplification in closely related
  • Author(s): Katoh S, Kaneko S, Isagi Y, Murakami N, Kato H
  • Journal Title: Conservation Genetics (accepted)
• [Presentation] トチノキ種子の花粉親の果実内および果実間での比較 (2008)
  • Author(s): 川口英之, 櫻澤如一, 名波哲, 松木悠, 井鷺裕司
  • Organizer: 日本森林学会第119回大会
  • Place of Presentation: 東京農工大学府中キャンパス体育館
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Comparison of pollen parents of seed set of Aesculus turbinata among fruits and individuals (2008)
  • Author(s): Kawaguchi, Y., Sakurazawa, Y., Nanami, S., Matsuki, Y., Isagi, Y
  • Organizer: The 119th Annual Meeting of the Japanese Forest Society
  • Place of Presentation: Tokyo University of Agriculture and Technology, Fuchu campus
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ホオノキ果実内の種子自殖率・遺伝的多様性の個体・果実による変動 (2008)
  • Author(s): 松木悠, 舘野隆之輔, 柴田銃江, 井鷺裕司
  • Organizer: 日本生態学会第55回大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 三宅島の噴火がヤブツバキの繁殖をめぐる花粉媒介系に与えた影響-鳥類が運ぶ花粉粒の直接遺伝解析 (2008)
  • Author(s): 阿部晴恵, 上野真義, 山本裕, 陶山佳久, 津村義彦, 長谷川雅美
  • Organizer: 日本生態学会第55回大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Variation of selfing rate and genetic diversity of Magnolia obovata among individuals and fruits (2008)
  • Author(s): Matsuki, Y., Tateno, R., Shibata, M., Isagi, Y
  • Organizer: The 54th Annual Meeting of the Ecological Society of Japan
  • Place of Presentation: Fukuoka International Congress Center
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of eructation of Mt. Miyake on pollination system of Cameria japonica-direct genetic analysis of pollen grains transported by birds (2008)
  • Author(s): Abe, H., Ueno, M., Yamamoto, Y., Suyama, Y., Tsumura, Y., Hasegawa, M.
  • Organizer: The 54th Annual Meeting of the Ecological Society of Japan
  • Place of Presentation: Fukuoka International Congress Center
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ホオノキ果実内の種子自殖率・遺伝的多様性の個体・年による変動 (2008)
  • Author(s): 松木悠、舘野隆之輔、柴田銃江、井鷺裕司
  • Organizer: 第55回日本生態学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-03-17
• [Presentation] クリの訪花昆虫に付着した花粉一粒ずつのDNA分析による送粉パターン発表の解析 (2008)
  • Author(s): 長谷川陽一, 陶山佳久, 清和研二
  • Organizer: 日本生態学会第55回大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-03-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Analysis of pollination patterns of Castanea crenata as determined by DNA analysis of single pollen grains adhered to insects (2008)
  • Author(s): Hasegawa, Y., Suyama, Y., Seiwa, K
  • Organizer: The 54th Annual Meeting of the Ecological Society of Japan
  • Place of Presentation: Fukuoka International Congress Center
  • Year and Date: 2008-03-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] クリの訪花昆虫に付着した花粉一粒ずつのDNA分析による送粉パターンの解析 (2008)
  • Author(s): 長谷川陽一・陶山佳久・清和研二
  • Organizer: 第55回日本生態学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-03-16
• [Presentation] 三宅島の噴火がヤブツバキの繁殖をめぐる花粉媒介系に与えた影響-鳥類が運ぶ花粉粒の直接遺伝解析. (2008)
  • Author(s): 阿部晴恵・上野真義・山本裕・陶山佳久・津村義彦・長谷川雅美
  • Organizer: 第55回日本生態学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-03-15
• [Presentation] 森林の断片化がホオノキの繁殖成功に与える影響 (2007)
  • Author(s): 舘野隆之輔, 松木悠, 井鷺裕司, 柴田銃江
  • Organizer: 日本森林学会第118回大会
  • Place of Presentation: 九州大学箱崎キャンパス国際ホール
  • Year and Date: 2007-04-03
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effect of forest fragmentation on reproduction success of Magnolia obovata (2007)
  • Author(s): Tetano, R., Matsuki, Y., Isagi, Y., Shibata, M
  • Organizer: The 118th Annual Meeting of the Japanese Forest Society
  • Place of Presentation: Kyushu University, Hakozald campus
  • Year and Date: 2007-04-03
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 花粉1粒の直接遺伝解析から何を見るか-1粒でもわかる事と1粒だからわかる事 (2007)
  • Author(s): 井鷺裕司
  • Organizer: 日本生態学会第54回大会
  • Place of Presentation: 愛媛大学共通教育講義棟
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 花粉一粒のDNA分析方法 (2007)
  • Author(s): 陶山佳久
  • Organizer: 日本生態学会第54回大会
  • Place of Presentation: 愛媛大学共通教育講義棟
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 花粉1粒の直接遺伝解析に基づくホオノキ訪花昆虫の送粉効率評価 (2007)
  • Author(s): 松木悠, 舘野隆之輔, 柴田銃江, 陶山佳久, 井鷺裕司
  • Organizer: 日本生態学会第54回大会
  • Place of Presentation: 愛媛大学共通教育講義棟
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] クリ花粉一粒ずつのマイクロサテライト分析による花粉流動解析 (2007)
  • Author(s): 長谷川陽一, 陶山佳久, 清和研二
  • Organizer: 日本生態学会第54回大会
  • Place of Presentation: 愛媛大学共通教育講義棟
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ハイマツ・キタゴヨウ交雑帯における交配パターン:花粉分散から種子形成まで (2007)
  • Author(s): 綿野泰行, 朝川毅守, 伊東めぐみ, 陶山佳久
  • Organizer: 日本生態学会第54回大会
  • Place of Presentation: 愛媛大学共通教育講義棟
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 昆虫付着花粉の遺伝解析を用いた熱帯林一斉開花における送粉システムの解明 (2007)
  • Author(s): 近藤俊明, 西村千, 内藤洋子, 津村義彦, 井鷺裕司, 奥田敏統, Lee Soon Leong, Norwati Muhammad
  • Organizer: 日本生態学会第54回大会
  • Place of Presentation: 愛媛大学共通教育講義棟
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] What can we show using the method of direct genetic analysis of single pollen grain (2007)
  • Author(s): Isagi, Y
  • Organizer: The 54th Annual Meeting of the Ecological Society of Japan
  • Place of Presentation: Ehime University
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The Method of DNA analysis of single pollen gain (2007)
  • Author(s): Suyama, Y
  • Organizer: The 54th Annual Meeting of the Ecological Society of Japan
  • Place of Presentation: Ehime University
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Pollination efficiencies of insects that visited to Magnolia obovata as determined by direct genetic analysis of pollen grains (2007)
  • Author(s): Matsuki, Y., Tateno, R., Shibata, M., Suyama, Y., Isagi, Y
  • Organizer: The 54th Annual Meeting of the Ecological Society of Japan
  • Place of Presentation: Ehime University
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Analysis of pollen dispersal pattern using microsatellite analysis of pollen grains of Castanea crenata (2007)
  • Author(s): Hasegawa, Y., Suyama, Y., Seiwa, K
  • Organizer: The 54th Annual Meeting of the Ecological Society of Japan
  • Place of Presentation: Ehime University
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Mating pattern in hybrid zone of Pinus pumila and Pinus parviflora var. pentaphylla-from pollen dispersal to seed production (2007)
  • Author(s): Watano, Y., Asakawa, T., Ito, M., Suyama, Y
  • Organizer: The 54th Annual Meeting of the Ecological Society of Japan
  • Place of Presentation: Ehime University.
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Clarification of pollination system in masting of tropical forest using the genetic analysis of insect adherent pollen grains (2007)
  • Author(s): Kondo, T., Nishimura, S., Naito, Y., Tsumura, Y., Isagi, Y., Okuda, T., Leong, LS., Muhammad, N
  • Organizer: The 54th Annual Meeting of the Ecological Society of Japan
  • Place of Presentation: Ehime University
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 体表付着花粉の遺伝解析によるホオノキ訪花昆虫の送粉特性解析 (2006)
  • Author(s): 松木悠, 井鷺裕司, 陶山佳久, 舘野隆之輔, 柴田銃江
  • Organizer: 日本森林学会第117回大会
  • Place of Presentation: 東京農業大学世田谷キャンパス1号館
  • Year and Date: 2006-04-03
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Analysis of pollination efficiencies of insects that visited to Magnolia obovata using genetic analysis of pollen grain. (2006)
  • Author(s): Matsuki, Y., Isagi, Y., Suyama, Y., Tateno, R., Shibata, M
  • Organizer: The 117th Annual Meeting of the Japanese Forest Society
  • Place of Presentation: Tokyo University of Agriculture, Setagaya campus
  • Year and Date: 2006-04-03
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 花粉一粒ずつを対象としたDNA分析によるクリの送粉パターンの解明 (2006)
  • Author(s): 長谷川陽一, 陶山佳久, 清和研二
  • Organizer: 日本生態学会第53回大会
  • Place of Presentation: 新潟コンベンションセンター
  • Year and Date: 2006-03-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Analysis of pollination pattern of Castanea crenata using DNA analysis of single pollen grains (2006)
  • Author(s): Hasegawa, Y., Suyama, Y., Seiwa, K
  • Organizer: The 53rd Annual Meeting of the Ecological Society of Japan
  • Place of Presentation: Niigata Convention Center
  • Year and Date: 2006-03-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 体表付着花粉の遺伝解析でホオノキ訪花昆虫の送粉効率を評価する (2006)
  • Author(s): 松木悠, 井鷺裕司, 陶山佳久, 舘野隆之輔, 柴田銃江
  • Organizer: 日本生態学会第53回大会
  • Place of Presentation: 新潟コンベンションセンター
  • Year and Date: 2006-03-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Evaluation of pollination efficiency by direct genetic analysis of pollen grains adhering to insects (2006)
  • Author(s): Matsuki, Y., Isagi, Y., Suyama, Y., Tateno, R., Shibata, M
  • Organizer: The 53rd Annual Meeting of the Ecological Society of Japan
  • Place of Presentation: Niigata Convention Center.
  • Year and Date: 2006-03-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Sustanability and Diversity of Forest Ecosystems -An Interdisciplinary Approach (2008)
  • Author(s): Isagi, Y., Tateno, R., Matsuki, Y., Hirao, A., Watanabe, S., and Shibata, M.
  • Total Pages: 86
  • Publisher: Springer
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Sustainability and Biodiversity Assessment on Forest Utilization Options (2008)
  • Author(s): Matsuki, Y. and Isagi, Y.
  • Total Pages: 258
  • Publisher: Research Institute for Humanity and Nature
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Sustainability and Biodiversity Assessment on Forest Utilization Options (2008)
  • Author(s): Tateno, R., Matsuki, Y., Isagi, Y., Shibata, M.
  • Total Pages: 258
  • Publisher: Research Institute for Humanity and Nature
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Sustainability and Biodiversity Assessment on Forest Utilization Options (2008)
  • Author(s): Shibata, M., Kikuchi, S., Tanaka, H., Yoshimaru, H., Niiyama, K.
  • Total Pages: 258
  • Publisher: Research Institute for Humanity and Nature
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Genetic and reproductive consequences of forest fragmentation for populations of Magnolia obovata (2008)
  • Author(s): Isagi, Y., Tateno, R., Matsuki Y, Hirao, A., Watanabe, S., Shibata, M
  • Total Pages: 86
  • Publisher: Nakashizuka, T. (ed.) Sustanability and Diversity of Forest Ecosystems -An Interdisciplinary Approach
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Direct genetic analysis of single pollen grains in pollination studies (2008)
  • Author(s): Matsuki, Y., Isagi, Y
  • Total Pages: 25
  • Publisher: In : M. Ichikawa, S Yamashita, T. Nakashizuka (eds.) Sustainability and Biodiversity Assessment on Forest Utilization Options
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] The effects of forest fragmentation on population structure and reproductive output in populations of Magnolia obovata (2008)
  • Author(s): Tateno, R., Matsuki, Y., Isagi, Y., Shibata, M
  • Total Pages: 258
  • Publisher: M. Ichikawa, S Yamashita, T. Nakashizuka Sustainability and Biodiversity Assessment on Forest Utilization Options
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Effects of human disturbance of forest on reproduction of a heterodichogamous maple, Acer mono (2008)
  • Author(s): Shibata, M., Kikuchi, S., Tanaka, H., Yoshimaru, H., Niiyama, K
  • Total Pages: 258
  • Publisher: In : M. Ichikawa, S Yamashita, T. Nakashizuka Sustainability and Biodiversity Assessment on Forest Utilization Options
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 「森の芽生えの生態学」 (2008)
  • Author(s): 陶山佳久
  • Total Pages: 258
  • Publisher: 文一総合出版