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Evaluation of health checking and vital activity on the basis of functional profiles of the host defense molecules of the cultured oysters in the three species of the genera Crassostrea

Research Project

Project/Area Number 17380112
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionTohoku University

Principal Investigator

TAKAHASHI Keisuke G  Tohoku University, Tohoku University, Graduate School of Agricultural Science, Associate Professor (80240662)

Co-Investigator(Kenkyū-buntansha) SUZUKI Tohru  Tohoku University, Graduate School of Agricultural Science, Professor (70344330)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,000,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥900,000)
Fiscal Year 2007: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2006: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2005: ¥7,100,000 (Direct Cost: ¥7,100,000)
KeywordsHost defense mechanisms / Crassostrea gigas / Oyster / Vital ability / Lysozyme / Hemocyte / Phagocytosis / Gene expression / 貧食能 / 液性生体防御因子
Research Abstract

The authors investigated for the host defense mechanisms in bivalve molluscs, especially, phagocytosis and generation of the reactive oxygen intermediates (ROIs) by hemocytes, and determined activities of lysozyme and lectins in the three species of the genera Crassostrea, C. gigas, C. nippona, and C. sikamea. Oyster hemocytes were divided into two morphological types: granulocytes and agranulocytes. The ratios between the granulocytes and agranulocytes varied during the year, however, the number of agranulocytes was always greater than that of granulocytes; approximately 70% of the total cell population was composed of agranulocytes. Granulocytes were more active phagocytes against three species of bacteria tested. Yeast cells were also extensively phagocytized by the granulocytes, but the agranulocytes produced a little phagocytosis of yeast cells. Granulocytes produced large amount of superoxide anion after phorbol stimulation or zymosan phagocytosis, but agranulocytes did not or on … More ly slightly produced superoxide anion. A simple method called the phagocytic plaque assay was used to visually present and estimate the phagocytic ability of the hemocytes from the giant Pacific oyster, Crassostrea gigas, using adherent Staphylococcus aureus cells on a plastic dish.
Crassostrea gigas hemocytes might phagocytose yeast cells by recognizing and binding to polysaccharides on the cell wall surface. This study was conducted to determine the differences in phagocytic capacity of C. gigas hemocytes for heat-killed yeast, zymosan, and zymocel, which have similar in size and shape, but which differ in their compositions of polysaccharides on the particle surface. Both agranulocytes and granulocytes exerted strong phagocytic capacity for all tested particles. Especially, zymosan was greatly phagocytosed by some of the granulocytes. To determine whether the phagocytosis of these respective particles depended on their recognition by specific polysaccharide receptors on the hemocyte surface, C. gigas hemocytes were pretreated with soluble a-mannan or laminarin and made to phagocytose the three types of the particles. The percentage of phagocytic cells of laminarin-treated granulocytes decreased significantly for zymosan and zymocel, but not for yeast. These results suggest that C. gigas might have multiple types of hemocytes with phagocytic capacity, some of which depend on β-glucan receptor(s) for phagocytosis.
For this study, we purified a novel isoform of the Manila clam lectin (designated MCL-4) from the plasma of the Manila clam, Ruditapes philippinarum, using affinity chromatography and gel filtration. Native PAGE results showed that the MCL-4 consisted of 70 kDa protein. The MCL-4 was found to be composed of 58-kDa and 43-kDa bands when examined using SDS-PAGE under reducing and non-reducing conditions. The native MCL-4 was revealed as a 147 kDa molecular mass protein by gel filtration. The purified MCL-4 agglutinates calcium-dependently in the erythrocytes of sheep and rabbit, but not in cells of the three species of marine bacteria tested. However, the phagocytic ability of the R. philippinarum hemocytes for the MCL-4-opsonized Vibrio tubiashii cells was significantly greater than that for the BSS-treated bacterial cells. Addition of purified MCL-4 markedly suppressed Alteromonas haloplanktis growth. These results suggest that MCL-4, because of its opsonizing and bacteriostatic properties, might contribute to the host defense mechanisms against invading microorganisms in R. philippinarum. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (58 results)

All 2008 2007 2006 2005

All Journal Article (37 results) (of which Peer Reviewed: 19 results) Presentation (21 results)

  • [Journal Article] Mechanisms and immunological roles of apoptosis in molluscs.2008

    • Author(s)
      K. Terahara and K. G. Takahashi
    • Journal Title

      Cur. Pharm. Des. 14

      Pages: 131-137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Identification and tissue expression analysis of C-type lectin and gale ctin in the Pacific oyster, Crassostrea gigas.2008

    • Author(s)
      K. Yamaura, K. G. Takahashi, and T. Suzuki
    • Journal Title

      Comp. Biochem. Physiol. 149B

      Pages: 168-175

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Purification and antibacterial characterization of a novel isoform of the Manila clam lectin (MCL-4) from the plasma of the Manila clam, Ruditapes philippinarum.2008

    • Author(s)
      K. G. Takahashi, T. Kuroda, and K. Muroga
    • Journal Title

      Comp. Biochem. Physiol. 150B

      Pages: 45-52

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 二枚貝の細胞性生体防御機構.2008

    • Author(s)
      高橋計介・室賀清邦
    • Journal Title

      魚病研究 43

      Pages: 1-17

    • NAID

      10021214575

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Phagocytic capacity for yeast and yeast cell wall derivatives by hemo cytes from the Pacific oyster, Crassostrea gigas.2008

    • Author(s)
      K. G. Takahashi
    • Journal Title

      Aquaculture 276(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Distribution of multiple peptidoglycan recognition proteins in the tissues of Pacific oyster, Crassostrea gigas.2008

    • Author(s)
      N. Itoh and K. G. Takahashi
    • Journal Title

      Comp. Biochem. Physiol. 151B(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Mechanisms and immunological roles of apoptosis in molluscs2008

    • Author(s)
      K. Terahara and K. G. Takahashi
    • Journal Title

      Cur. Pharm. Des. 14

      Pages: 131-137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification and tissue expression analysis of C-type lectin and galectin in the Pacific oyster, Crassostrea gigas2008

    • Author(s)
      K. Yamaura, K. G. Takahashi, and T. Suzuki
    • Journal Title

      Comp. Biochem. Physiol. 149B

      Pages: 168-175

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Purification and antibacterial characterization of a novel isoform of the Manila clam lectin (MCL-4) from the plasma of the Manila clam, Ruditapes philippinarum2008

    • Author(s)
      K. G. Takahashi, T. Kuroda, and K. Muroga
    • Journal Title

      Comp. Biochem. Physiol. 150B

      Pages: 45-52

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cellular defense mechanisms in bivalve molluscs2008

    • Author(s)
      K. G. Takahashi and K. Muroga
    • Journal Title

      Fish Pathol. 43

      Pages: 1-17

    • NAID

      10021214575

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Phagocytic capacity for yeast and yeast cell wall derivatives by hemocytes from the Pacific oyster, Crassostrea gigas2008

    • Author(s)
      K. G. Takahashi
    • Journal Title

      Aquaculture 276(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Distribution of multiple peptidoglycan recognition proteins in the tissues of Pacific oyster, Crassostrea gigas2008

    • Author(s)
      N. Itoh and K. G. Takahashi
    • Journal Title

      Comp. Biochem. Physiol. 151B(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification and tissue expression analysis of C-type lectin and galectin in the Pacific oyster, Crassostrea gigas.2008

    • Author(s)
      K. Yamaura, K. G. Takahashi, and T. Suzuki
    • Journal Title

      Comp. Biochem. Physiol. 149B

      Pages: 168-175

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and antibacterial characterization of a novel isoform of the Manila clam lectin(MCL-4) from the plasma of the Manila clam, Ruditapes philippinarum.2008

    • Author(s)
      K. G. Takahashi, T. Kuroda, and K. Muroga
    • Journal Title

      Comp. Biochem. Physiol. 150B

      Pages: 45-52

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phagocytic capacity for yeast and yeast cell wall derivatives by hemocytes from the Pacific oyster, Crassostrea gigas.2008

    • Author(s)
      K. G. Takahashi
    • Journal Title

      Aquaculture 277(in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] cDNA cloning and in situ hybridization of a novel lysozyme in the Pacific oyster, Crassostrea gigas.2007

    • Author(s)
      N. Itoh and K. G. Takahashi
    • Journal Title

      Comp. Biochem. Physiol. 148B

      Pages: 160-166

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 二枚貝の液性生体防御因子に関するに関する研究の歴史と現状.2007

    • Author(s)
      室賀清邦・高橋計介
    • Journal Title

      魚病研究 42

      Pages: 1-17

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] cDNA cloning and in situ hybridization of a novel lysozyme in the Pacific oyster, Crassostrea gigas2007

    • Author(s)
      N. Itoh and K. G. Takahashi
    • Journal Title

      Comp. Biochem. Physiol. 148B

      Pages: 160-166

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Overview on the studies of humoral defense factors in bivalve molluscs2007

    • Author(s)
      K. Muroga and K. G. Takahashi
    • Journal Title

      Fish Pathol. 42

      Pages: 1-17

    • NAID

      10018909102

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Norovirus contamination in wild oysters and mussels in Shiogama Bay, northeastern Japan.2007

    • Author(s)
      Maekawa, Miura, Kato, K. G. Takahashi and K. Muroga
    • Journal Title

      J. Shellfish Res. 26

      Pages: 281-286

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 二枚貝の液性生体防御因子に関するに関する研究の歴史と現状.2007

    • Author(s)
      室賀清邦・高橋計介
    • Journal Title

      魚病研究. 42

      Pages: 1-17

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] cDNA cloning and in situ hybridization of a novel lysozyme in the Pacific oyster, Crassostrea gigas.2007

    • Author(s)
      Itoh and K. G. Takahashi
    • Journal Title

      Comp. Biochem. Physiol. 148B

      Pages: 160-166

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Difference in integrin-dependent phagocytosis among three hemocyte subpopulations from the Pacific oyster Crassostrea gigas.2006

    • Author(s)
      K. Terahara, K. G. Takahashi, A. M. Nakamura, M. Osada, M. Yoda, T. Hiroi, M. Hirasawa, and K. Mori
    • Journal Title

      Dev. Comp. Immunol. 30

      Pages: 667-683

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cloning of cDNAs and hybirdization analysis of lysozymes from two oyster species, Crassostrea gigas and Ostrea edulis.2006

    • Author(s)
      T. Matsumoto, A. M. Nakamura, and K. G. Takahashi
    • Journal Title

      Comp. Biochem. Physiol. 145B

      Pages: 325-330

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Difference in integrin-dependent phagocytosis among three hemocyte subpopulations from the Pacific oyster Crassostrea gigas2006

    • Author(s)
      K. Terahara, K. G. Takahashi, A. M. Nakamura, M. Osada, M. Yoda, T. Hiroi, M. Hirasawa, and K. Mori
    • Journal Title

      Dev. Comp. Immunol. 30

      Pages: 667-683

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cloning of cDNAs and hybridization analysis of lysozymes from two oyster species, Crassostrea gigas and Ostrea edulis2006

    • Author(s)
      T. Matsumoto, A. M. Nakamura, and K. G. Takahashi
    • Journal Title

      Comp. Biochem. Physiol. 145B

      Pages: 325-330

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Phagocytic ability of oyster hemocytes measured by a simple method using bacterial thin-layer2006

    • Author(s)
      Takahashi, KG, et al.
    • Journal Title

      The Tohoku J Agr. Res. 56

      Pages: 85-91

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Differences in integrin-dependent phagocytosis among three hemocyte subpopulations of the Pacific oyster "Crassostrea gigas"2006

    • Author(s)
      Takahashi, KG, et al.
    • Journal Title

      Dev. Comp. Immunol. 30

      Pages: 667-683

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cloning of cDNAs and hybridization analysis of lysozymes from two oyster species, Crassostrea gigas and Ostrea edulis2006

    • Author(s)
      Takahashi, KG, et al.
    • Journal Title

      Com. Biochem. Physiol. 145

      Pages: 325-330

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Differences in integrin-dependent phagocytosis among three hemocyte subpopulation of the pacific oyster "Crassostrea gigas"2006

    • Author(s)
      Terahara, Takahashi, et al.
    • Journal Title

      Dev.Comp.Immunol. (Article in Press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Phagocytic ability of oyster hemocytes measured by a simple method using bacterial thin-layer.2005

    • Author(s)
      K. G. Takahashi
    • Journal Title

      Tohoku J. Agricult. Res. 55

      Pages: 85-92

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] イワガキおよびマガキ血球の形態と貪食能2005

    • Author(s)
      高橋計介・矢内秀和・室賀清邦・森 勝義
    • Journal Title

      水産増殖 53

      Pages: 53-59

    • NAID

      10015597216

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Pacific oyster hemocytes undergo apoptosis following cell-adhesion mediated by integrin-like molecules.2005

    • Author(s)
      K. Terahara, K. G. Takahashi, and K. Mori
    • Journal Title

      Comp. Biochem. Physiol. 141A

      Pages: 215-222

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Phagocytic ability of oyster hemocytes measured by a simple method using bacterial thin-layer2005

    • Author(s)
      K. G. Takahashi
    • Journal Title

      Tohoku J. Agricult. Res. 55

      Pages: 85-92

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Morphology and phagocytic activity of hemocytes from the Japanese rock oyster Crassostrea nippona and the Pacific oyster C. gigas.2005

    • Author(s)
      K. G. Takahashi, H. Yanai, K. Muroga, and K. Mori
    • Journal Title

      Aquaculture Sci. 53

      Pages: 53-59

    • NAID

      10015597216

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Pacific oyster hemocytes undergo apoptosis following cell-adhesion mediated by integrin-like molecules2005

    • Author(s)
      K. Terahara, K. G. Takahashi, and K. Mori
    • Journal Title

      Comp. Biochem. Physiol. 141A

      Pages: 215-222

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] pacific oyster hemocytes undergo apoptosis following cell-adhesion mediated by integrin-like molecules.2005

    • Author(s)
      Terahara, Takahashi, et al.
    • Journal Title

      Comp.Biochem.Physiol. 141(2)

      Pages: 215-222

    • Related Report
      2005 Annual Research Report
  • [Presentation] Protein kinase C regulation of cell spreading the Pacific oyster hemocytes2007

    • Author(s)
      K. G. Takahashi
    • Organizer
      The Annual Meeting of the Japanese Society of Fisheries Science Autumn 2007
    • Place of Presentation
      Hakodate
    • Year and Date
      2007-09-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マガキ血球の接着・伸展に対するプロテインキナーゼCの関与2007

    • Author(s)
      高橋計介
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      北海道大学水産学部
    • Year and Date
      2007-09-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] マガキ血球の接着・伸展に対するプロテインキナーゼCの関与.2007

    • Author(s)
      高橋計介
    • Organizer
      日本水産学会 2007年度秋季大会
    • Place of Presentation
      函館
    • Year and Date
      2007-09-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マガキ血球による酵母および酵母細胞壁成分の貪食機構.2007

    • Author(s)
      高橋計介
    • Organizer
      第4回東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2007-06-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マガキの消化盲嚢における複数のリゾチーム発現.2007

    • Author(s)
      伊藤直樹・高橋計介
    • Organizer
      第4回東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2007-06-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Phagocytic capacity for yeast and yeast cell wall derivatives by hemocytes from the Pacific oyster, Crassostrea gigas2007

    • Author(s)
      K. G. Takahashi
    • Organizer
      The 4^<th> Bioscience Symposium of Tohoku University
    • Place of Presentation
      Sendai
    • Year and Date
      2007-06-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Gene expression and tissue distribution of multiple lysozymes in the Pacific oyster2007

    • Author(s)
      N. Itoh and K. G. Takahashi
    • Organizer
      The 4^<th> Bioscience Symposium of Tohoku University
    • Place of Presentation
      Sendai
    • Year and Date
      2007-06-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マガキが有する3種類のレクチン遺伝子の塩基配列と組織発現.2007

    • Author(s)
      山浦邦宏・高橋計介・鈴木 徹
    • Organizer
      日本水産学会 2007年度大会
    • Place of Presentation
      東京
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ムラサキイガイにおけるリゾチーム活性の季節変動と体内分布.2007

    • Author(s)
      高橋計介・齋藤彩華・室賀清邦
    • Organizer
      日本水産学会 2007年度大会
    • Place of Presentation
      東京
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification and tissue expression analysis of C-type lectin and galectin in the Pacific oyster, Crassostrea gigas.2007

    • Author(s)
      K. Yamaura, K. G. Takahashi, and T. Suzuki
    • Organizer
      The Annual Meeting of the Japanese Society of Fisheries Science Spring 2007
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Seasonal changes and tissue distribution of lysozyme in the blue mussel2007

    • Author(s)
      K. G. Takahashi, A. Saitoh, and K. Muroga
    • Organizer
      The Annual Meeting of the Japanese Society of Fisheries Science Spring 2007
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 細菌刺激によるマガキ組織のリゾチーム、血リンパのレクチンおよび酵素活性の変動.2007

    • Author(s)
      高橋計介・鈴木兵衛・室賀清邦
    • Organizer
      日本水産学会 2007年度大会
    • Place of Presentation
      東京
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Changes in lysozyme, lectin, and enzymatic activities in the hemolymph of the Pacific oyster2007

    • Author(s)
      K. G. Takahashi, H. Suzuki, and K. Muroga
    • Organizer
      The Annual Meeting of the Japanese Society of Fisheries Science Spring 2007
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マガキ血リンパにおける酵素活性の測定.2006

    • Author(s)
      木原智彦・高橋計介・室賀清邦
    • Organizer
      日本水産学会 2006年度大会
    • Place of Presentation
      高知
    • Year and Date
      2006-03-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マガキにおける殺菌活性の季節変動とリゾチームの体内分布.2006

    • Author(s)
      高橋計介・伊木誠一郎・伊藤淑恵・室賀清邦
    • Organizer
      日本水産学会 2006年度大会
    • Place of Presentation
      高知
    • Year and Date
      2006-03-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Enzymatic activity in the hemolymph of the Pacific oyster2006

    • Author(s)
      T. Kihara, K. G. Takahashi, and K. Muroga
    • Organizer
      The Annual Meeting of the Japanese Society of Fisheries Science 2006
    • Place of Presentation
      Kochi
    • Year and Date
      2006-03-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Seasonal changes and tissue distribution of lysozyme in the Pacific oyster2006

    • Author(s)
      K. G. Takahashi, S. Iki, Y. Ito, and K. Muroga
    • Organizer
      The Annual Meeting of the Japanese Society of Fisheries Science 2006
    • Place of Presentation
      Kochi
    • Year and Date
      2006-03-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mass mortalities and defense mechanisms in oysters.2005

    • Author(s)
      K. G. Takahashi
    • Organizer
      The 1^<st> International Oyster Symposium
    • Place of Presentation
      Tokyo
    • Year and Date
      2005-07-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Enzymatic activity in the hemocytes and hemolymph of the Pacific oyster.2005

    • Author(s)
      K. G. Takahashi, T. Kihara, T. Kuroda, and K. Muroga
    • Organizer
      The 1^<st> International Oyster Symposium
    • Place of Presentation
      Tokyo
    • Year and Date
      2005-07-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mass mortalities and defense mechanisms in oysters2005

    • Author(s)
      K. G. Takahashi
    • Organizer
      The 1^<st> International Oyster Symposium
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2005-07-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Enzymatic activity in the hemocytes and hemolymph of the Pacific oyster2005

    • Author(s)
      K. G. Takahashi, T. Kihara, T. Kuroda, and K. Muroga
    • Organizer
      The 1^<st> International Oyster Symposium
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2005-07-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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