Appearance of causative dinoflagellates for diarrheatic shellfish poisoning following the blooms of certain cryptophyte species
| Project/Area Number |
17380118
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hiroshima University (2007)
Kitasato University (2005-2006) |
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Principal Investigator |
KOIKE Kazuhiko Hiroshima University, Graduate School of Biosphere Science, Associate Professor (30265722)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥12,880,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥9,100,000 (Direct Cost: ¥9,100,000)
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| Keywords | diarrheatic shellfish poisoning / Dinophysis / Sanriku Coast / cryptophyte / Teleaulax / intracellular symbiont / toxic dinoflagellate / Amylax / 三陸 / Dinophysis fortii / 貝毒 / 葉緑体 / 定量PCR / クレプトプラスト / in situハイブリダイゼーション |
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| Research Abstract |
Study aim of this research is to know whether bloom of a causative dinoflagellate for diarrheatic shellfish poisoning (Dinophysis fortii) could follow the appearance of its plastid-source microalga. In our previous study, it has been clarified that the plastid (chloroplast) of D. fortii can be originated from a cryptophyte alga, namely Teleaulax sp.
Cells of Teleaulax were detected by a technique of fluorescent in situ hybridization (FISH) using specific molecular probe, and with an automated epifluorescent microscope. On the two-years surveys in Okkirai Bay, Sanriku Coast, the bloom of D. forth always occurred after that of Teleaulax. From the analysis of water temperature and salinity ranges which support Teleaulax blooming, the species can grow at the water formed by Tsugaru Warm Water Current and coastal surface water. In 2008, quantitative real-time PCR to detect the Teleaulax occurrence was developed and applied to the field survey. From these studies, the prediction of D. fortii bloom and subsequent toxin contamination to shellfish is now achieved by monitoring the Teleaulax occurrence.
During the above surveys, the following results, which may apart from the study topic but relate to the incidence of shellfish poisoning or Dinophysis itself, were obtained and reported; (1) Occurrence of yessotoxin producing dinoflagellate, Protoceratium reticulatum in Okkirai Bay, and related environmental factors, (2) Teleaulax vestiges in a gonyaulacalean dinoflagellate Amylax buxus and A. triacantha.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Yessotoxin analogues in several strains of .Protoceratium reticulatum in Japan determined by liquid chromatography-hybrid triple quadrupole/linear ion trap mass spectrometry2007
Author(s)
Suzuki, T., Hone, Y., Koike, K., Satake, M., Oshima, Y., Iwataki, M., Yoshimatsu, S
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Journal Title
J. Chrornatogr. A 1142
Pages: 172-177
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Mechanism of gamete fusion in Dinophysis fortii (Dinophyceae, Dinophyta) : light microscopic and ultrastructural observations2006
Author(s)
Koike, K., Nishiyama, A, Saitoh, K., Imai, K., Koike, K., Kobiyama, A., Ogata, T
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Journal Title
J. Phycol 42
Pages: 1247-1256
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A novel type of kleptoplastidy in Dinophysis (Dinophceae) : presence of haptophyte-type plastid in Dinophysis mitra2005
Author(s)
Koike, K., Sekiguchi, H., Kobiyama, A., Takishita, K., Kawachi, M., Koike, K., Ogata, T
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Journal Title
Protist 156
Pages: 225-237
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Confirmation of yessotoxin production by the dinoflagellate, Protoceratium reticulatum, in Mutsu Bay2005
Author(s)
Eiki, K., Satake, M., Koike, K., Ogata, T., Mitsuya, T., Oshima, Y
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Journal Title
Fish. Sci 71
Pages: 633-638
NAID
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Mechanisms of gamete fusion in Dinophysis fortii2006
Author(s)
A., Nishiyama, K., Saitoh, K., Imai, K., Koike, A., Kobiyama, T., Ogata
Organizer
Annual meeting of Japanese Fisheries Society
Place of Presentation
Kochi University
Year and Date
2006-04-01
Description
「研究成果報告書概要(欧文)」より
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