| Project/Area Number |
17380126
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Fisheries chemistry
|
|---|
| Research Institution | Fisheries Research Agency |
|---|
Principal Investigator |
CHO Naritoshi Fisheries Research Agency, National Researth Institute Fisheries Science, 研究室長 (70360766)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKEYAMA Haruko Waseda University, 理工学術院, 教授 (60262234)
OOHATA Ichiro National Researth Institute Fisheries Science, 中央水産研究所・水産遺伝子解析センター, 上席研究員 (40371843)
UENO Yasuhiro Tohoku Regional Fisheries Research Institute, 東北区水産研究所・資源生態研究室, 研究室長 (50371810)
YOSHIMURA Taku Seikai Regional Fisheries Researth Institute, 西海区水産研究所・沿岸資源研究室, 研究室長 (90371997)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥7,140,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
|
|---|
| Keywords | simultaneous identification / multi-species / fisheries animlas / ITS region / DNA chip |
|---|
| Research Abstract |
Sample size collected from wide variety of marine animals reached to 1,000 species at the end of 2007. Among them, we attempted to amplify the homologous. ITS1 region in 775 individuals from 600 species and observed successful single fragment amplification in 688 individuals. We determined entire or partial nucleotide sequences of 315 individuals from 273 species. All these sequences determined have been deposited to the database. We investigated the length and GC content of the ITS1 region that may have significant correlation with hybridization efficiency. No species was found to have ITS1shorter than 100 bp. In general, ITS1 of vertebrate(fishes) was GC richer(56.8 to 78 %) and longer(318 to 2300 bp) than invertebrates. Specifically, gelatinous animal(Cnidana and Ctenophora) were observed to have shorter ITS1(118 to 422 bp) with lower GC content(35.8 to 61.7 %) than the other animal taxa. Mollusca and Crustacea were diverse group in ITSl size, ranging from 108 to 1118 bp for the former and from 182 to 1613 bp for the later. No apparent relationship between the length and GC content was observed. The prototype chip designed last year was spotted with PCR products, and we could not get better discrimination results. In this year, we designed oligo probes(c. a. 60 nt) from 200 species, which do not share any similar nucleotide sequences one another, and these probes were spotted on the chip. Hybridization experiment using PCR products with this chip indicated the efficiency for species-specific hybridization was considerably improved.
|
