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Functional and expression analysis of genes involving sugar metabolism in forage grasses

Research Project

Project/Area Number 17380163
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionHokkaido University

Principal Investigator

YAMADA Toshihiko  Hokkaido Univ., Field Science Center for Northern Biosphere, Professor, 北方生物圏フィールド科学センター, 教授 (70343952)

Co-Investigator(Kenkyū-buntansha) TAMURA Ken-ichi  National Agriculture and Food Research Organization, National Agricultural Research Center for Hokkaido Region, Researcher, 食品産業総合技術研究機構・北海道農業研究センター, 研究員 (10414749)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 2006: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2005: ¥4,900,000 (Direct Cost: ¥4,900,000)
Keywordsforage grasses / sugar metabolism / RNAi / winter hardiness / transcriptional factor / transgenic / DNA marker / 転写遺伝子 / フルクタン
Research Abstract

The objectives of the present research are as follows: 1) Production of gene-silencing plants of forage grass, Lolium perenne with respect to genes involving fructan biosynthesis using RNA interference (RNAi) procedure to clarify the mechanism of functions of fructan biosynthesis in grasses, 2) Isolation of transcriptional factors for cool-inducible genes and elucidation of their function in grasses, 3) Development of available DNA markers for breeding programs of grasses based on genomic information of genes of sugar metabolism.
We obtained some results from two years research. For RNAi experiment, we made dsRNA expression vectors for three genes involving fructan biosynthesis which were isolated from L. perenne. The vectors were introduced into embryogenic calli derived from seeds of L. perenne using particle bombardment or Agrobacterium methods. Then calli with antibiotic resistance were obtained. Analysis of gene expression confirmed RNAi in calli, however such calli were not regenerated into plants. Ten novel putative CBFcDNAs of L. perenne (LpCBF genes) were isolated from cold-treated leaf tissue. Some CBF homolog genes were mapped on LG5 forming a cluster. Based on comparative genetic studies, conserved synteny for CBF gene family was observed between the Triticeae cereals and perennial ryegrass. RT-PCR analysis revealed that the expression of LpCBF genes was rapidly induced in response to low temperature and that the expression pattern under the low temperature conditions for a long period was different between the various LpCBF genes. Genes involving sucrose synthase and sucrose phosphate synthase as well as some cold inducible genes were isolated and sequence analysis made DNA markers distinguishable genotypes.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (18 results)

All 2007 2006 2005

All Journal Article (16 results) Book (2 results)

  • [Journal Article] A perennial ryegrass CBF gene cluster is located in a region predicted by conserved synteny between Poaceae species2007

    • Author(s)
      K.Tamura, T.Yamada
    • Journal Title

      Theoretical and Applied Genetics 114

      Pages: 273-283

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A perennial ryegrass CBF gene cluster is located in a region predicted by conserved synteny between Poaceae species.2007

    • Author(s)
      K.Tamura, T.Yamada
    • Journal Title

      Theoretical and Applied Genetics 114

      Pages: 273-283

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] CBF genes and QTLs for cold hardiness in perennial ryegrass2007

    • Author(s)
      K.Tamura, S.Kobayashi, T.Yamada
    • Journal Title

      Proceedings of XXVI Meeting of the EUCARPIA Fodder Crops and Amenity Grasses Section and XVI Meeting of the EUCARPIA Medicago spp. Group

      Pages: 284-289

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A perennial ryegrass CBF gene cluster is located in a region predicted by conserved synteny between Poaceae species2007

    • Author(s)
      K.Tamura, K.Tase, Y.Sanada, T.Komatsu, T.Yamada
    • Journal Title

      Plant & Animal Genome XV, Jan 13-17, 2006, San Diego, USA

      Pages: 270-270

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Gene expression and genetic mapping analyses of a perennial ryegrass glycine-rich RNA-binding protein gene suggest a role in cold adaptation2006

    • Author(s)
      H.Shinozuka, H.Hisano, S.Yoneyama, Y.Shimamoto, T.Yamada, A.Kanazawa
    • Journal Title

      Molecular General Genomics 275

      Pages: 399-408

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Sucrose metabolism of perennial ryegrass in relation to cold acclimation2006

    • Author(s)
      P.K.Bhowmik, K.Tamura, Y.Sanada, K.Tase, T.Yamada
    • Journal Title

      Zeitschrift fur Naturforschung 61C

      Pages: 99-104

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sucrose metabolism of perennial ryegrass in relation to cold acclimation.2006

    • Author(s)
      P.K.Bhowmik, K.Tamura, Y.Sanada, K.Tase, T.Yamada
    • Journal Title

      Zeitschrift fur Naturforschung 61C

      Pages: 99-104

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ペレニアルライグラスから単離された低温応答転写因子CBF遺伝子2006

    • Author(s)
      田村健一, 山田敏彦, 田瀬和浩, 眞田康治, 小松敏憲
    • Journal Title

      日本草地学会誌 52(別2)

      Pages: 270-271

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genetic association between winter hardiness and sugar content in perennial ryegrass (Lolium perenne L.)2006

    • Author(s)
      T.Yamada, S.Kobayashi, H.Hisano, K.Tamura, Y.Sanada, P.K.Bhowmik, M.Yoshida, I.P.Armstead, M.O.Humphreys
    • Journal Title

      Plant & Animal Genome XIV, Jan 14-18, 2006, San Diego, USA

      Pages: 15-15

    • Related Report
      2005 Annual Research Report
  • [Journal Article] イネ科牧草の越冬性に関するQTL解析2006

    • Author(s)
      山田敏彦, 小林創平, 久野裕, 眞田康治, 田村健一
    • Journal Title

      日本草地学会大会(H18年3月発表予定) (H18年3月発表予定)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular cloning and genetic mapping of perennial ryegrass casein protein kinase 2 α subunit genes2005

    • Author(s)
      H.Shinozuka, H.Hisano, R.C.Ponting, N.O.I.Cogan, E.S.Jones, J.W.Forster, T.Yamada
    • Journal Title

      Theoretical and Applied Genetics 112

      Pages: 167-177

    • NAID

      120000956150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Genetics and Molecular Breeding in Lolium/Festuca grass species complex2005

    • Author(s)
      T.Yamada, T., J.W.Forster, M.W.Humphreys, T.Takamizo
    • Journal Title

      Grassland Science 51

      Pages: 89-106

    • NAID

      80017607140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular cloning and *genetic mapping of perennial ryegrass casein protein kinase 2 < subunit genes.2005

    • Author(s)
      H.Shinozuka, H.Hisano, R.C.Ponting, N.O.I.Cogan, E.S.Jones, J.W.Forster, T.Yamada
    • Journal Title

      Theoretical and Applied Genetics 112

      Pages: 167-177

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetics and Molecular Breeding in Lolium/Festuca grass species complex.2005

    • Author(s)
      T.Yamada, T., J.W.Forster, M.W.Humphreys, T.Takamizo
    • Journal Title

      Grassland Science 51

      Pages: 89-106

    • NAID

      80017607140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetics and Molecular Breeding in Lolium/Festuca grass, species complex2005

    • Author(s)
      T.Yamada, T., J.W.Forster, M.W.Humphreys, T.Takamizo
    • Journal Title

      Grassland Science 51

      Pages: 89-106

    • NAID

      80017607140

    • Related Report
      2005 Annual Research Report
  • [Journal Article] QTL analysis and trait dissection in ryegrasses (Lolium spp.)2005

    • Author(s)
      T.Yamada, F.W.Forster
    • Journal Title

      The 4^<th> International Symposium on the Molecular Breeding of Forage and Turf, July 3-7, 2005, Aberystwyth, UK

    • NAID

      120000954954

    • Related Report
      2005 Annual Research Report
  • [Book] QTL analysis and trait dissection in ryegrasses (Lolium spp.). Molecular Breeding for the genetic improvement of forage crops and turf (Ed M.O. Humphreys)2005

    • Author(s)
      T.Yamada, F.W.Forster
    • Total Pages
      286
    • Publisher
      Wageningen Academic Publishers
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] QTL analysis and trait dissection in ryegrasses (Lolium spp.)(Ed. M.O.Humphreys)Molecular Breeding for the Genetic Improvement of Forage Crops and Turf2005

    • Author(s)
      T.Yamada, F.W.Forster
    • Total Pages
      286
    • Publisher
      Wageningen Academic Publishers
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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