The development of methods for evaluating embryo qualitybased on the electrochemical measuring technique

• Project/Area Number: 17380164
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Yamagata University (2008); Tohoku University (2005-2007)
• Principal Investigator: ABE Hiroyuki Yamagata University, 大学院・理工学研究科, 准教授 (10375199)
• Co-Investigator(Kenkyū-buntansha): HITOSHI Shiku 東北大学, 大学院・環境科学研究科, 准教授 (10361164) ; YOKOO Masaki 東北大学, 未来医工学治療開発センター, 助教 (10396541)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,350,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥1,350,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2006: ¥4,200,000 (Direct Cost: ¥4,200,000); Fiscal Year 2005: ¥6,300,000 (Direct Cost: ¥6,300,000)
• Keywords: 電気化学計測 / 細胞機能評価 / 細胞呼吸 / ミトコンドリア / 体外受精 / 細胞呼吸計測 / 走査型電気化学顕微鏡 / 家畜生殖細胞 / 体外培養 / 先端計測機器 / 電気化学イメージング / 体外成熟 / 電気化学イメージング技術 / 体外受精・胚培養 / 先端計測機器開発

Research Abstract

本研究では、電気化学的イメージング技術を基盤とする細胞呼吸計測システムを開発し、これを応用した受精卵品質評価法の開発を目的とした。走査型電気化学顕微鏡をベースに、非侵襲的・高感度で家畜胚や卵子の呼吸量を測定できる「細胞呼吸計測システム」の開発に成功した。この計測システムを用い、呼吸活性を指標に品質の高い家畜受精卵を選別できる新しい評価法を確立した。

Research Products

• [Journal Article] A birth from the transfer of a single vitrified-warmed blastocyst using ICSI with calcium ionophore oocyte activation in a globozoospermic patient (2009)
  • Author(s): Kanako Nakajo
  • Journal Title: Fertility and Sterility (印刷中)
  • Peer Reviewed
• [Journal Article] microfluidic dual capillary probe to collect mRNA from adherent cells and spheroids (2009)
  • Author(s): Hitoshi Shiku
  • Journal Title: Analytical Biochemistry (印刷中)
  • Peer Reviewed
• [Journal Article] Morphometric and ultrastructural changes in ciliated cells of the oviductal epithelium in prolific Chinese Meishan and Large White pigs during the estrous cycle (2008)
  • Author(s): Abe H., Hoshi H.
  • Journal Title: Reprod Domest Anim 43 Pages: 66-73
  • Peer Reviewed
• [Journal Article] Influence of hyaluronan accumulation during cumulus expansion on in vitro porcine oocyte maturation (2008)
  • Author(s): Yokoo M., Kimura N., Abe H., Sato E.
  • Journal Title: Zygote 16 Pages: 309-314
  • Peer Reviewed
• [Journal Article] Evaluating the embryo quality with a non-invasive and highly sensitive measurement of respiration activity by scanning electrochemical microscopy (2008)
  • Author(s): Hiroyuki Abe
  • Journal Title: Journal of Embryo Transfer 23(1) Pages: 29-37
• [Journal Article] Evaluating the quality of human embryos with a measurement of oxygen consumption by scanning electrochemical microscopy (2008)
  • Author(s): Takafumi Utsunomiya
  • Journal Title: Journal of Mammalian Ova Research 25 Pages: 2-7
  • Peer Reviewed
• [Journal Article] Measurement of the respiratory activity of single human embryos by scanning electrochemical microscopy (2008)
  • Author(s): Hiroyuki Abe
  • Journal Title: Trans. of MRS-J 33(3) Pages: 759-762
  • NAID: 130007809493
  • Peer Reviewed
• [Journal Article] Multiple analysis of respiratory activity in the identical oocytes by applying scanning electrochemical microscopy (2008)
  • Author(s): Masaki Yokoo
  • Journal Title: Trans. of MRS-J 33(3) Pages: 763-766
  • NAID: 130007809492
  • Peer Reviewed
• [Journal Article] 走査型電気化学顕微鏡による体外培養胚のクオリティ評価 (2008)
  • Author(s): 伊達安基
  • Journal Title: バイオサイエンスとインダストリー 66(6) Pages: 298-299
• [Journal Article] 畜産領域における呼吸測定による胚品質評価の展望 (2008)
  • Author(s): 阿部宏之
  • Journal Title: 新しい畜産技術-研究開発の成果と展開方法- Pages: 23-24
• [Journal Article] Blastocyst production from in vitro-produced Day-2 bovine embryos classified by cleavage stage, and cytogenetical of the resultant Day-8 blastocysts (2008)
  • Author(s): Carlos Manuel Ulloa Ulloa
  • Journal Title: Journal of Reproduction and Development 54 Pages: 465-474
  • Peer Reviewed
• [Journal Article] Influence of hyaluronan accumulation during cumulus expansion on in vitro porcine oocyte maturation (2008)
  • Author(s): Masaki Yokoo
  • Journal Title: Zygote 16 Pages: 309-314
  • Peer Reviewed
• [Journal Article] Mitochondria are well developed in pink-eyed dilution melanoblasts in the neonatal mouse evidermis (2008)
  • Author(s): Tomohisa Hirobe
  • Journal Title: Zoological Science 25 Pages: 1057-1065
  • Peer Reviewed
• [Journal Article] Morphometric and ultrastructural changes in ciliated cells of the oviductal epithelium in prolific Chinese Meishan and Large White pigs during the oestrus cycle (2008)
  • Author(s): Hiroyuki Abe
  • Journal Title: Reprod. Dom. Anim. 43 Pages: 66-73
  • Peer Reviewed
• [Journal Article] Measurement of the respiratory activity of single human embryos by scanning electrochemical microscopy (2008)
  • Author(s): Hiroyuki Abe
  • Journal Title: Transactions of the Materials Research Society of Japan 31(In press)
  • NAID: 130007809493
  • Peer Reviewed
• [Journal Article] Multiple analysis of respiratory activity in the identical cells by applying electrochemical measuring technology (2008)
  • Author(s): Masaki Yokoo
  • Journal Title: Transactions of the Materials Research Society of Japan 31(In press)
  • Peer Reviewed
• [Journal Article] Analysis of mRNA from single adherent cells and spheroids collected using fast electrical lysis (2007)
  • Author(s): Nashimoto Y., Takahashi Y., YamakawaT., Torisawa Y., Yasukawa T., Ito-SasakiT., Yokoo M., Abe H., Shiku H., Kambara H., Matsue T.
  • Journal Title: Anal.Chem. 79 Pages: 6823-6830
  • Peer Reviewed
• [Journal Article] Excess tyrosine rescues the reduced activity ofproliferation and differentiation of cultured recessive yellow melanocytes derived from neonatal mouse epidermis (2007)
  • Author(s): Hirobe T., Abe H., Wakamatsu K., Ito S.,Kawa Y., Soma Y., Mizoguchi M.
  • Journal Title: Eur J Cell Biol 86 Pages: 315-330
  • Peer Reviewed
• [Journal Article] Regional and cyclic variations in the ultrastructural features of secretory cells in the oviductal epithelium of the Chinese Meishan pig (2007)
  • Author(s): Abe H., Hoshi H.
  • Journal Title: Reprod Domest Anim 42 Pages: 292-298
  • Peer Reviewed
• [Journal Article] A microfluidic chip integrated with amperometric detector array for in situ estimating oxygen consumption characteristics of single bovine embryo (2007)
  • Author(s): Wu C.-C., Saito T., Yasukawa T., Shiku H., Abe H., Hoshi H., Matsue T.
  • Journal Title: Sens Actuat B 125 Pages: 680-687
  • Peer Reviewed
• [Journal Article] A non-invasive and sensitive method for measuring cellular respiration with a scanning electrochemical microscopy to evaluate embryo quality (2007)
  • Author(s): Hiroyuki Abe
  • Journal Title: J. Mamm. Ova Res. 24 Pages: 70-78
  • NAID: 10019804045
  • Peer Reviewed
• [Journal Article] Regional and cyclic variations in the ultrastructural features of secretory cells in the oviductal epithelium of the Chinese Meishan pig (2007)
  • Author(s): Hiroyuki Abe
  • Journal Title: Reprod. Dom. Anim. 42 Pages: 292-298
  • Peer Reviewed
• [Journal Article] 電気化学的イメージング法を応用した単一ヒト胚の呼吸能解析 (2007)
  • Author(s): 阿部宏之
  • Journal Title: 産婦人科の実際 56 Pages: 2053-2057
• [Journal Article] Estimation of respiratory activity of individual human embryos by scanning electrochemical microscopy (2007)
  • Author(s): Hiroyuki Abe
  • Journal Title: International Proceedings of ISIVF2007 Pages: 171-174
• [Journal Article] Respiratory activity and ultrastructural features of bovine somatic nuclear transfer embryos (2007)
  • Author(s): Hiroyuki Abe
  • Journal Title: Reproduction, Fertility and Development 19・1 Pages: 129-130
• [Journal Article] The efficient maintenance of respiratory activity of oocytes relates to the high competence of oocyte maturation in pigs (2007)
  • Author(s): Masaki Yokoo
  • Journal Title: Reproduction, Fertility and Development 19・1 Pages: 295-295
• [Journal Article] Morphometric and ultrastructural changes in ciliated cells of the oviductal epithelium in prolific Chinese Meishan and Large White pigs during the estrous cycle (2007)
  • Author(s): Hiroyuki Abe
  • Journal Title: Reproduction in Domestic Animals (In press)
• [Journal Article] Role of hyaluronan receptor CD44 during in vitro maturation in pigs (2007)
  • Author(s): Masaki Yokoo
  • Journal Title: Journal of Reproduction and Development (In press)
• [Journal Article] A microfluidic chip integrated with amperometric detector array for in situ estimating oxygen consumption characteristics of single bovine embryo (2007)
  • Author(s): C.-C.Wu
  • Journal Title: Sensors and Actuators B (In press)
• [Journal Article] Effects of hyaluronan on oxygen consumption and ATP content in blastocysts produced in vitro (2007)
  • Author(s): Koji Yoshioka
  • Journal Title: Reproduction, Fertility and Development 19・1 Pages: 215-215
• [Journal Article] Evaluation of mitochondrial function in single cloned miniature pig embryos by measuring oxygen consumption (2007)
  • Author(s): Satoru Moriyasu
  • Journal Title: Reproduction, Fertility and Development 19・1 Pages: 219-219
• [Journal Article] Evaluation of mitochondrial function in single cloned miniature pig embryos by measuring oxygen consumption (2007)
  • Author(s): Satoshi Sugimura
  • Journal Title: Reproduction, Fertility and Development 19・1 Pages: 161-161
• [Journal Article] Oxygen consumption of cell suspension in a poly(dimethlsiloxane)(PDMS)microchannel estimated by scanning electrochemical microscopy (2006)
  • Author(s): Saito T., Wu C.-C., Shiku H., Yasukawa T., Yokoo M., Sasaki T., Abe H., Hoshi H.,Matsue T.
  • Journal Title: Analyst 131 Pages: 1006-1011
  • Peer Reviewed
• [Journal Article] Evaluating the quality of individual embryos with a non-invasive and highly sensitive measurement of oxygen consumption by scanning electrochemical microscopy (2006)
  • Author(s): Hiroyuki Abe
  • Journal Title: Journal of Reproduction and Development 55・suppl
• [Journal Article] Oxygen consumption of cell suspension in a poly(dimethlsiloxane) (PDMS) microchannel estimated by scanning electrochemical microscopy (2006)
  • Author(s): Takeshi Saito
  • Journal Title: Analyst 131 Pages: 1006-1011
• [Journal Article] 呼吸量測定によるウシ体外培養胚の品質評価と半自動計測装置の開発 (2006)
  • Author(s): 青柳重夫
  • Journal Title: 分析化学 55・11 Pages: 847-854
• [Journal Article] Estimation of respiratory activity of mouse embryos by scanning electrochemical microscopy (2006)
  • Author(s): Takahiro Sasaki
  • Journal Title: Zoological Science 23・12 Pages: 1185-1185
• [Journal Article] Analysis of respiratory activity of single bovine oocytes by scanning electrochemical microscopy (2006)
  • Author(s): Hiroyuki Abe
  • Journal Title: Proceeding of the 4^<th> International Forum on Post-genome Technologies Pages: 19-22
• [Journal Article] Microsystem surveying three dimensional (3D) culture and embryo development (2006)
  • Author(s): Hitoshi Shiku
  • Journal Title: Proceeding of the 4^<th> International Forum on Post-genome Technologies Pages: 221-223
• [Journal Article] A quality evaluation system for embryos and oocytes using scanning electrochemical microscopy (2006)
  • Author(s): Hiroyuki Abe
  • Journal Title: Proceedings CD of The XII^<th> AAAP Animal Congress 2006 C03-LS-01 Pages: 1-6
• [Journal Article] Oxygen permeability of surface-modified poly(dimethylsiloxane) characterized by scanning electrochemical microscopy (2006)
  • Author(s): Hitoshi Shiku
  • Journal Title: Chemistry Letters 35 Pages: 235-236
  • NAID: 10017167481
• [Journal Article] Oxygen consumption of bovine cumulus cells and oocytes cultured in different culture systems for oocyte maturation (2006)
  • Author(s): Hiroyuki Abe
  • Journal Title: Reproduction, Fertility and Development 18 Pages: 267-267
• [Journal Article] 電気化学的イメージング技術を応用した胚のクオリティー評価 (2006)
  • Author(s): 阿部宏之
  • Journal Title: 産婦人科の実際 55・2 Pages: 207-216
• [Journal Article] Relationship between oxygen consumption and sex of bovine in vitro fertilized embryos (2005)
  • Author(s): B.Argung
  • Journal Title: Reproduction in Domestic Animals 40 Pages: 51-56
• [Journal Article] Metabolic and enzymatic activity of individual cells, spheroids, and embryos as a function of the sample size (2005)
  • Author(s): Hitoshi Shiku
  • Journal Title: Sensors and Actuators B 108 Pages: 597-602
• [Journal Article] Ordered progress of spermiogenesis to the fertilizable sperm of the medaka fish, Oryzias latipes, in cell culture (2005)
  • Author(s): Takahiro Sasaki
  • Journal Title: Development, Growth and Differentiation 47 Pages: 87-97
• [Journal Article] Respiration activity of bovine embryos cultured in serum-free and serum-containing media (2005)
  • Author(s): Hiroyuki Abe
  • Journal Title: Reproduction, Fertility and Development 17 Pages: 215-215
• [Journal Article] Estimation of oxygen consumption of mouse oocytes during maturation by scanning electrochemical microscopy (2005)
  • Author(s): Takahiro Sasaki
  • Journal Title: Zoological Science 22・6 Pages: 1453-1453
• [Journal Article] Estimation of oxygen consumption of Medaka (Oryzias latipes) embryos by scanning electrochemical microscopy (2005)
  • Author(s): Hiroyuki Abe
  • Journal Title: Zoological Science 22・6 Pages: 1460-1460
• [Journal Article] 受精卵呼吸測定装置の開発と応用 (2005)
  • Author(s): 阿部宏之
  • Journal Title: 化学と生物 43 Pages: 709-711
• [Journal Article] Regional and cyclic variations in the ultrastructural features of secretory cells in the oviductal epithelium of the Chinese Meishan pig
  • Author(s): Hiroyuki Abe
  • Journal Title: Reproduction in Domestic Animals (In press)
• [Presentation] Non-invasive and highly sensitive measurement of the respiratory activity of individual human embryos by scanning electrochemical microscopy (2008)
  • Author(s): Hiroyuki Abe
  • Organizer: The 64^<th> Annual Meeting of American Society for Renroductive Medicine
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-11-10
• [Presentation] Evaluating the quality of single human embryos with a non-invasive and highly sensitive measurement of respiration activity by scanning electrochemical microscopy (2008)
  • Author(s): Hiroyuki Abe
  • Organizer: The 3^rd International Workshop on Approaches to Single-Cell-Analysis
  • Place of Presentation: Zurich, Switzerland
  • Year and Date: 2008-09-12
• [Presentation] Evaluating the quality of single human embryos with a non-invasive and highly sensitive measurement of respiration activity by scanning electrochemical microscopy (2008)
  • Author(s): Abe H
  • Organizer: The 3rd International Workshop on Approaches to Single-Cell-Analysis
  • Place of Presentation: Zurich, Switzerland(invited)
  • Year and Date: 2008-09-11
• [Presentation] ブタ体外受精胚の呼吸活性と胚発生能 (2008)
  • Author(s): 横尾正樹
  • Organizer: 日本動物学会平成20年度東北支部大会
  • Place of Presentation: 盛岡市・盛岡市観光文化交流センター
  • Year and Date: 2008-07-26
• [Presentation] valuating the embryo quality with a non-invasive and highly sensitive measurement of respiration activity by scanning electrochemical microscopy (2008)
  • Author(s): Hiroyuki Abe
  • Organizer: The Korean Society of Embryo Transfer 2008
  • Place of Presentation: Korea
  • Year and Date: 2008-05-23
• [Presentation] 新しい培養評価法-受精卵呼吸解析による培養液の性能評価について- (2008)
  • Author(s): 阿部宏之
  • Organizer: 第49回日本哺乳動物卵子学会
  • Place of Presentation: 名古屋市・名古屋国際会議場
  • Year and Date: 2008-05-18
• [Presentation] マウス卵成熟および胚発生過程におけるCytochrome c oxidase遺伝子の発現変化 (2008)
  • Author(s): 横尾正樹
  • Organizer: 第49回日本哺乳動物卵子学会
  • Place of Presentation: 名古屋市・名古屋国際会議場
  • Year and Date: 2008-05-18
• [Presentation] 新しい培養評価法-受精卵呼吸解析による培養液の性能評価について- (2008)
  • Author(s): 阿部宏之
  • Organizer: 第49回日本哺乳動物卵子学会
  • Place of Presentation: 名古屋国際会議場(招待講演)
• [Presentation] Effects of bovine plasma lipoproteins on accumulationof cytoplasmic lipid droplets and mitochondrial morphology in bovine embryos (2008)
  • Author(s): Hiroyuki Abe
  • Organizer: 34th Annual Meeting of the International Embryo Transfer Society
  • Place of Presentation: ハイアットリージェンシーデンバー
• [Presentation] ブタ卵成熟過程における卵子ミトコンドリア機能の解析 (2007)
  • Author(s): 横尾正樹
  • Organizer: 第100回日本繁殖生物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2007-10-31
• [Presentation] 電気化学計測法による卵子呼吸能解析とクオリティー評価への応用 (2007)
  • Author(s): 阿部宏之
  • Organizer: 第52回日本生殖医学会
  • Place of Presentation: 秋田大学
  • Year and Date: 2007-10-25
• [Presentation] Estimation of respiratory activity of single human embryos by scanning electrochemical microscopy (2007)
  • Author(s): Abe H
  • Organizer: The 5th International Forum on Post-genome Technologies
  • Place of Presentation: Suzhou, China(invited)
  • Year and Date: 2007-10-11
• [Presentation] 呼吸代謝能解析による子卵子・胚の品質評価と応用 (2007)
  • Author(s): 阿部宏之
  • Organizer: 第25回日本受精着床学会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2007-08-30
• [Presentation] 微細加工技術と電気化学計測の複合システムによる精子呼吸能測定 (2007)
  • Author(s): 阿部宏之
  • Organizer: 第26回日本アンドロロジー学会
  • Place of Presentation: ディズニーアンバサダー
  • Year and Date: 2007-07-06
• [Presentation] マウス胚発生過程におけるミトコンドリア機能解析 (2007)
  • Author(s): 岡崎直人
  • Organizer: 第48回日本哺乳動物卵子学会
  • Place of Presentation: アピオ甲府
  • Year and Date: 2007-05-27
• [Presentation] 電気化学的細胞呼吸測定によるヒト胚用発生培地の性能評価 (2007)
  • Author(s): 横尾正樹
  • Organizer: 第48回日本哺乳動物卵子学会
  • Place of Presentation: アピオ甲府
  • Year and Date: 2007-05-27
• [Presentation] 無侵襲的呼吸計測技術を応用した多項目呼吸能解析 (2007)
  • Author(s): 横尾正樹
  • Organizer: 第46回日本生体医工学会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2007-04-25
• [Presentation] Non-invasive quality assessment of IVP embryos Post Conference Satellite Symposium (2007)
  • Author(s): Abe H
  • Organizer: The 33^<rd> Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: Kyoto, Japan(invited)
  • Year and Date: 2007-01-10
• [Presentation] 呼吸代謝能解析による卵子・胚の品質評価と応用 (2007)
  • Author(s): 阿部宏之
  • Organizer: 第25回日本受精着床学会
  • Place of Presentation: 仙台国際センター(招待講演)
• [Presentation] 電気化学計測法による卵子呼吸能解析とクオリティー評価への応用 (2007)
  • Author(s): 阿部宏之
  • Organizer: 第52回日本生殖医学会
  • Place of Presentation: 秋田県民会館(招待講演)
• [Book] 「マイクロ電極を用いた受精卵呼吸測定」、動物実験代替のためのバイオマテリアル開発 (2007)
  • Author(s): 阿部宏之
  • Publisher: シーエムシー出版
• [Book] 胚の呼吸能の測定、図説よくわかる臨床不妊症学入門・生殖補助医療編 (2007)
  • Author(s): 阿部宏之
  • Publisher: 中外医学社
• [Book] 動物実験代替のためのバイオマテリアル開発 (2007)
  • Author(s): 阿部宏之
  • Publisher: シーエムシー出版
• [Book] 胚の呼吸能の測定 (2007)
  • Author(s): 阿部宏之
  • Publisher: 中外医学社
• [Remarks] 2007年11月22日朝日新聞「不妊治療で元気な受精卵選別」
• [Remarks] 2009年1月15日山形新聞「元気な受精卵は呼吸が活発・不妊治療の成績向上に期待」
• [Patent(Industrial Property Rights)] 哺乳動物受精卵の呼吸活性測定方法及びその電気化学デバイス (2008)
  • Inventor(s): 珠玖仁、末永智一、高野真一郎、伊達安基、齋藤剛史、横尾正樹、伊藤隆広、阿部宏之
  • Industrial Property Rights Holder: クリノ(株)、東北大学、山形大学
  • Industrial Property Number: 2008-293117
  • Filing Date: 2008-11-15
• [Patent(Industrial Property Rights)] 哺乳動物受精卵の呼吸活性測定方法及びその電気化学デバイス (2008)
  • Inventor(s): 珠玖仁
  • Industrial Property Rights Holder: 東北大学・山形大学
  • Industrial Property Number: 2008-293117
  • Filing Date: 2008-11-15