Isolation of novel candidate genes in bovine adipocyte differentiation and marbling by subtraction method
| Project/Area Number |
17380165
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Tohoku University |
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Principal Investigator |
ASO Hisashi Tohoku University, Tohoku University, Graduate School of Agricultural Science, Associate Professor (50241625)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Takahiro Tohoku University, Graduate School of Agricultural Science, Professor (20111297)
OHWADA Shuichi Tohoku University, Graduate School of Agricultural Science, Assistant Professor (00183244)
WATANABE Koichi Tohoku University, Graduate School of Agricultural Science, Assistant Professor (80261494)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,840,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2006: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2005: ¥7,300,000 (Direct Cost: ¥7,300,000)
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| Keywords | Intramuscular preadipocyte / Adipogenesis / BIP cell / PIP cell / Bovine / Porcine / pGPx / Subtraction / 牛筋肉内脂肪前駆細胞株 / ブタ筋肉内脂肪前駆細胞株 / オクタン酸 / オレイン酸 / FAT / CD36 / FATP1 / 血清型glutathione peroxidase / C / EBPδ / 脂肪蓄積マーカー / デキサメサゾン / glutathione peroxidase (GOx-P) / α-2-マクログロブリン / stearoyl CoA desaturase / 血清アミロイドA蛋白3 |
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| Research Abstract |
Plasma glutathione peroxidase (pGPx) is an anti-oxidative enzyme. Using the polymerase chain reaction subtraction method, we have previously identified pGPx as a large part of the genes that are expressed following adipocyte differentiation in a bovine intramuscular preadipocyte (BIP) lne. The expression of pGPx and C/EBP δ increases during adipogenesis, with dexamethasone being the main effector of these genes. The expression of pGPx gene has been clearly detected in BIP cells and human adipocytes, but hardly in 3T3-L1 cells. The production of pGPx in bovine tissues is greatest in kidney and in intraperitoneal fat. We consider that the transcriptional control of pGPx in cattle might be carried out by C/EBP δ and that the expression of pGPx might be a characteristic phenomenon of bovine adipogenesis.
Next, to understand the relationship between intramuscular adipogenesis in the pig and the supply fatty acids, we established a clonal porcine intramuscular preadipocyte (PIP) line from the marbling muscle tissue of female Duroc pig. Confluent PIP cells exhibited a fibroblastic appearance. Their adipogenic ability was investigated using confluent PIP cells after exchanging growth medium for adipogenic medium containing 50 ng/mL insulin, 0.25 μM dexamethasone, 2 mM octanoate, and 200 μM oleate. When cells were cultured in differentiation medium for 8 days, large numbers of lipid droplets were observed in differentiated PIP cells, and their cytosolic TG content increased in a time-dependent manner. While oleate only induced the expression of PPARγ mRNA, but not that of C/EBPα, octanoate significantly induced the expression of both PPARγ and C/EBPα mRNA. Octanoate and oleate accelerated the inducing effect of insulin and dexamethasone on the expression of aP2 mRNA. These results indicate that a combination of octanoate and oleate synergistically induced PIP adipogenesis, and that the stimulation of octanoate was essential to the trigger for the adipogenesis in PIP cells.
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Report
(4 results)
Research Products
(56 results)
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[Presentation] Calcification needs the release of ANX II as well as ANX V from chondrocytes2005
Author(s)
Takeshi Minashima, Hisashi Aso, Kouichi Tahara, Tadashi Yamasaki, Syuichi Ohwada, Kouichi Watanabe, and Takahiro Yamaguchi
Organizer
Joint meeting of 2nd IS-AS and 3rd IS-IFS, The Tohoku Journal of Agricultural Research, 56 (1, 2): 42
Place of Presentation
Sendai, JAPAN
Year and Date
2005-11-17
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] The winding road to candidate genes for marbling2005
Author(s)
Hisashi Aso, Koji Tahara, Tadashi Yamasaki, Takeshi Minashima, Masato Sanosaka, Kohtaro Miyazawa, Shinichiro Hayashi, Takashi Kanaya, Koichi Watanabe, Shuichi Ohwada, Takahiro Yamaguchi
Organizer
Joint meeting of 2nd IS-AS and 3rd IS-IFS The Tohoku Journal of Agricultural Research, 56 (1, 2): 27
Place of Presentation
Sendai, JAPAN
Year and Date
2005-11-17
Description
「研究成果報告書概要(欧文)」より
Related Report
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