| Project/Area Number |
17380168
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Shinshu University |
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Principal Investigator |
ROH Sanggun Shinshu University, Faculty of Agriculture, Associated professor (90322130)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Shin-ichi Shinshu University, Faculty of Agriculture, Professor (00003682)
ONO Tamao Shinshu University, Faculty of Agriculture, Professor (10177264)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,530,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥930,000)
Fiscal Year 2007: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2006: ¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 2005: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | Bovine / Adipogenesis / Adipogenin / GPCR43 / GPCR120 / 脂肪細胞 / 脂肪組織 / GPR120 / Claudin-6 |
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| Research Abstract |
In order to build the system of good beef production of Japanese Black Cattle, understanding of fat accumulation among each adipose tissue is important. Therefore, the candidate genes related with fat depots have investigated in Japanese Black Cattle. (1) The gene of CDC42, prefoldin-5, decorin, phosphate carrier, 12S ribosomal RNA gene, and Kbtbd2 was identified by the analysis of the gene expression profile between subcutaneous and visceral adipose tissue using the differential display RT-PCR method. Expression patterns of these genes in Japanese Black Cattle were different with those of a single stomach animal like a pig or a mouse. (2) GPCR43, Adipogenin and GPCR120 were highly expressed in adipose tissue and up-regulated during adipocyte differentiation. GPCR43, Adipogenin, GPCR120, and Claudin-6 were important genes on adipocyte differentiation and adipose tissue development (4) Bovine Adipogenin isoform cDNA received alternatives splicing as a result of base sequence analysis, and it was confirmed that the base sequence is missing as compared with Adipogenin. Bovine isoform cDNA was highly expressed in adipose tissue and up-regulated during the adipocyte differentiation of bovine. This was similar with the results of the Adipogenin gene. A possibility that the isoform of an Adipogenin gene was involved with adipose tissue developments was suggested. (5) Introducing an Adipogenin gene into a C2C12 cell showed the fat accumulation in cells without the changes of genes related with adipogenesis.
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