Explorations and Functional Analysis of gene related with fat accumulation
| Project/Area Number |
17380168
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Shinshu University |
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Principal Investigator |
ROH Sanggun Shinshu University, Faculty of Agriculture, Associated professor (90322130)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Shin-ichi Shinshu University, Faculty of Agriculture, Professor (00003682)
ONO Tamao Shinshu University, Faculty of Agriculture, Professor (10177264)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,530,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥930,000)
Fiscal Year 2007: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2006: ¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 2005: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | Bovine / Adipogenesis / Adipogenin / GPCR43 / GPCR120 / 脂肪細胞 / 脂肪組織 / GPR120 / Claudin-6 |
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| Research Abstract |
In order to build the system of good beef production of Japanese Black Cattle, understanding of fat accumulation among each adipose tissue is important. Therefore, the candidate genes related with fat depots have investigated in Japanese Black Cattle. (1) The gene of CDC42, prefoldin-5, decorin, phosphate carrier, 12S ribosomal RNA gene, and Kbtbd2 was identified by the analysis of the gene expression profile between subcutaneous and visceral adipose tissue using the differential display RT-PCR method. Expression patterns of these genes in Japanese Black Cattle were different with those of a single stomach animal like a pig or a mouse. (2) GPCR43, Adipogenin and GPCR120 were highly expressed in adipose tissue and up-regulated during adipocyte differentiation. GPCR43, Adipogenin, GPCR120, and Claudin-6 were important genes on adipocyte differentiation and adipose tissue development (4) Bovine Adipogenin isoform cDNA received alternatives splicing as a result of base sequence analysis, and it was confirmed that the base sequence is missing as compared with Adipogenin. Bovine isoform cDNA was highly expressed in adipose tissue and up-regulated during the adipocyte differentiation of bovine. This was similar with the results of the Adipogenin gene. A possibility that the isoform of an Adipogenin gene was involved with adipose tissue developments was suggested. (5) Introducing an Adipogenin gene into a C2C12 cell showed the fat accumulation in cells without the changes of genes related with adipogenesis.
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Report
(4 results)
Research Products
(84 results)
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[Journal Article] Chemerin-A New adipokine that modulates adipogenesis via its own receptor2007
Author(s)
Roh, SG., Song, SH., Choi, KC., Katoh, K., Wittamer, V., Parmentier, M., Sasaki, S
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Journal Title
Biochem. Biophys. Res. Commun 362(4)
Pages: 1013-18
Description
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Kawanishi, T., Takahashi, H., Funai, S., Suzuki, M., Mizuno, A., Goto, C., Roh, SG., Sasaki, S., Tsuji, H., Hamano, K
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Journal Title
Hokushinetsu Journal of Animal Science 94
Pages: 35-41
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[Journal Article] Expression of FSH receptor mRNA in Goat seminiferous Epithelium2007
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Takahashi, H., Funai, S., Kawanishi, T., Goth, C., Rob, SG., Ono, T., Sasaki, S., Tsuji, H., Nakura, Y., Fujita, M., Hamano, K
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Hokushinetsu Journal'of Animal Science 94
Pages: 43-47
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[Journal Article] Roh SG (Corresponding Author), The regulation of adipogenesis through GPR1202007
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Gotoh, C., Hong, YH., Iga, T., Hishikawa, D., Suzuki, Y., Song, SH., Choi, KC., Adachi, T., Hirasawa, A., Tsujimoto, G., Sasaki, S
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Journal Title
Biochem. Biophys. Res. Commun 354(2)
Pages: 591-597
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[Journal Article] Identification of differentially expressed transcripts in bovine rumen and abomasum using a differnutial display method2007
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Roh, SG., Kuno, M., Hishikawa, D., Hong, YH., Katoh, K., Ohara, Y., Hidari, H., Sasaki, S
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Journal Title
J. Anim. Sci 85
Pages: 395-403
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[Journal Article] Z-chromosome specific primers for chicken-quail hybrid blastoderm2007
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Takagi, S., Ono, T., Tsukada, A., Atsumi, Y., Mizushima, S., Saito, N., Shimada, K
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Journal of Poultry Science 44(2)
Pages: 209-212
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[Journal Article] A novel method to isolate primordial germ cells and its use for the generation of germline chimeras in chicken2007
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Yamamoto, Y., Usui, F., Nakamura, Y., Ito, Y., Tagami, T., Nirasawa, K., Matsubara, Y., Ono, T., Kagami, H
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Biology of Reproduction 77(1)
Pages: 115-119
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[Journal Article] Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing a W chromosome2007
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Takagi, S., Ono, T., Tsukada, A., Atsumi, Y., Mizushima, S., Saito, N., Shimada, K
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Journal Title
Poultry Science 86(5)
Pages: 937-943
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[Journal Article] Migration and proliferation of primordial germ cells in the early chicken embryo2007
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Nakamura, Y., Yamamoto, Y., Mushika, T., Usui, F., Ono, T., Seitoko, AR., Takeda, K., Nirasawa, K., Kagami, H., Tagami, T
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Poultry Science 86(10)
Pages: 2182-2193
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[Journal Article] Differential susceptibility of rat embryos to methyl methanesulfonate during the pregastrulation period2007
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Yokoi, R., Suda, S., Kobayashi K., Kuroda, J., Kusama, H., Kagami, H., Ono, T
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Journal of Toxicological Sciences 32(5)
Pages: 495-503
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[Journal Article] Production of lean beef containing a high content of trans-10, cis-12 conjugated linoleic acid by feeding high-temperature-micro-time treated diet with extruded soybean2006
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Xu, CX., Lee, HG., Lee, BK., Wang, JH., Hong, ZS., Kim, TG., Kang, SK., Choi, NG., Roh, SG., Choi, Y.J
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Biosci Biotechnol. Biochem 70(11)
Pages: 2589-2597
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[Journal Article] Molecular cloning and expression analysis of cDNA encoding bovine adipogenin2006
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Hong, YH., Ogihara, Y., Hishikawa, D., Gotoh, C.,Iga, T., Suzuki, Y., Song, SH., Nakajima, K., Kozakai, T., Sasaki, S., Roh, SG
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Journal Title
Animal Science Journal 77
Pages: 613-619
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[Journal Article] The response of plasma leptin and feed intake to growth hormone administration in Holstein calves with different planes of nutrition2006
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Lee, HG., Hong, ZS., Kim, MK., Kang, SK., Xu, CX., Cho, JS., Seo, KS., Roh, SG., Choi, YJ
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Journal Title
Can. J. Anim. Sci 86
Pages: 225-233
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[Journal Article] Reduction in voltage-gated K+ currents in primary cultured rat pancreatic β-cells by linoleic acids2006
Author(s)
Feng, DD., Luo, Z., Roh, SG., Hernandez, M., Tawadoros, N., Chen, C
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Journal Title
Endocrinology 147
Pages: 674-682
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[Journal Article] Selective decrease of chick embryonic primordial germ cells in vivo and in vitro by soft X-ray irradiation2006
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Lim, JM., Kwon, HM., Kim, DK., Kim, JN., Park, TS., Ono, T., Han, JY
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Journal Title
Animal Reproduction Science 95(1-2)
Pages: 67-74
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[Journal Article] Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR432005
Author(s)
Hong, YH., Nishimura, Y., Hishikawa, D., Tsuzuki, H., Miyahara, H., Gotoh, C., Choi, KC., Fung, D., Chen, C., Lee, HG., Katoh, K., Roh, SG., Sasaki, S
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Endocrinology 146
Pages: 5092-5099
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[Journal Article] Up-regulation of the claudin-6 gene in adipogenesis2005
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Hong, YH., Hishikawa, D., Miyahara, H., Nishimura, Y., Tsuzuki, H., Gotoh, C., Iga, T., Suzuki, Y., Song, SH., Choi, KC., Lee, HG., Sasaki, S., Roh, SG., (Corresponding Author)
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Biosci. Biotechnol. Biochem 69(11)
Pages: 2117-2121
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[Journal Article] Identification of genes expressed differentially in subcutaneous and visceral fat of cattle, pig and mouse2005
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Hishikawa, D., Hong, YH., Roh, SG., Miyahara, H., Nishimura, Y., Tomimatsu, A., Tsuzuki, H., Gotoh, C., Kuno, M., Choi, KC., Lee, HG., Cho, KK., Hidari, H., Sasaki, S
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Physiological Genomics 21(3)
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[Journal Article] Altered cytokine expression in mesenteric lymph nodes in a rat strain (MES) that spontaneously develops hypereosinophilia2005
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Immunology 116(3)
Pages: 373-380
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[Journal Article] Up-regulation of adipogenin, an adipocyte plasma transmembrane protein, during adipogenesis2005
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Hong, YH., Hishikawa, D., Miyahara, H., Tsuzuki, H., Nishimura, Y., Gotoh, C., Choi, KC., Hokari, Y., Takagi, Y., Lee, HG., Cho, KK., Roh, SG., Sasaki, S
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Journal Title
Molecular and Cellular Biochemistry 276
Pages: 133-141
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[Journal Article] Inhibition of GH secretagogue receptor antagonist, [D-Lys-3]-GHRP-6, in adipogenesis of ovine and rat adipocytes2005
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Roh, SG., Hong, YH., Hishikawa, D., Tsuzuki, H., Miyahara, H., Nishimura, Y,. Gotoh, C., Suzuki, Y., Choi, KC., Lee, HG., Sasaki, S
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Animal Science Journal 76
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