Development of blastocysts from porcine primordial follicles following xenografting to nude mice

• Project/Area Number: 17380170
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: KANEKO Hiroyuki National Institute of Agrobiological Sciences, 生殖機構研究ユニット, 上級研究員 (60343993)
• Co-Investigator(Kenkyū-buntansha): KIKUCHI Kazuhiro 独立行政法人農業生物資源研究所, 動物科学研究領域・生殖機構研究ユニット, 主任研究員 (20360456) ; 柏崎 直巳 麻布大学, 獣医学部, 教授 (90298232)
• Co-Investigator(Renkei-kenkyūsha): KASHIWAZAKI Naomi 麻布大学, 獣医学部, 教授 (90298232)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥15,360,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥1,560,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2006: ¥5,200,000 (Direct Cost: ¥5,200,000); Fiscal Year 2005: ¥3,400,000 (Direct Cost: ¥3,400,000)
• Keywords: 発生工学 / 原始卵胞卵 / 異種間移植 / 体外培養系 / 胚発生 / 応用動物 / 発生・分化 / 未成熟生殖細胞 / 核置換 / ホルモン処理 / 原始卵胞

Research Abstract

卵巣に存在する原始卵胞卵は雌側の遺伝情報を伝える重要な資源であるが、未だ活用されていない。この研究では、ブタをモデルとして異種間移植、体外培養および顕微操作を組み合わせて、原始卵胞卵を人為的に発育させ、さらに初期胚へと発生させることに成功した。これら一連の成果は、稀少な動物あるいは家畜の原始卵胞卵から個体を再生する上で、必要不可欠な手法である。

Research Products

• [Journal Article] Nuclear replacement of in vitro-matured porcine oocytes by a serial centrifugation and fusion method. (2009)
  • Author(s): Maedomari N, Kikuchi K, Nagai T, Fahrudin M, Kaneko H, Noguchi J, Nakai M, Ozawa M, Somfai T, Nguyen LV, Ito J, Kashiwazaki N.
  • Journal Title: Reprod Domest Anim (in press)
  • Peer Reviewed
• [Journal Article] Generation of porcine diploid blastocysts after injection of spermatozoa grown in nude mice. (2009)
  • Author(s): Nakai M, Kaneko H, Somfai T, Maedomari N, Ozawa M, Noguchi J, Kashiwazaki N, Kikuchi K
  • Journal Title: Theriogenology (in press)
  • Peer Reviewed
• [Journal Article] Generation porcine diploid blastocysts after injection of spermatozoa grown in nude mice (2009)
  • Author(s): Nakai, M, et al.
  • Journal Title: Theriogenology (in press)
  • Peer Reviewed
• [Journal Article] Endocrine status and development of porcine testicular tissues in host mice. (2008)
  • Author(s): Kaneko H, Kikuchi K, Nakai M, Noguchi J.
  • Journal Title: J Reprod Dev 54 Pages: 480-485
  • NAID: 10024940463
  • Peer Reviewed
• [Journal Article] Selected aspects of advanced porcine reproductive technology. (2008)
  • Author(s): Kikuchi K, Kashiwazaki N, Nagai T, Nakai M, Somfai T, Noguchi J, Kaneko H.
  • Journal Title: Reprod Domest Anim 43(Suppl 2) Pages: 401-406
  • Peer Reviewed
• [Journal Article] Endocrine status and development of porcine testicular tissues in host mice (2008)
  • Author(s): Kaneko, H, et al.
  • Journal Title: Journal of Reproduction and Development 54 Pages: 480-485
  • NAID: 10024940463
  • Peer Reviewed
• [Journal Article] Selected aspects of advanced porcine reproductive technology (2008)
  • Author(s): Kikuchi, K, et al.
  • Journal Title: Reproduction in Domestic Animals 43(Suppl. 2) Pages: 401-406
  • Peer Reviewed
• [Journal Article] Xenografting of porcine immature germ cells and their developmental competence. (2007)
  • Author(s): Kaneko H, Kikuchi K, Nakai M, Maedomari N, Kashiwazaki N.
  • Journal Title: J Reprod Engineering 10(Suppl) Pages: 436-448
  • Peer Reviewed
• [Journal Article] Xenografting of porcine immature germ cells and their developmental competence (2007)
  • Author(s): Kaneko, H., et. al.
  • Journal Title: Journal of Reproduction and Engineering 10(Suppl.) Pages: 436-448
  • Peer Reviewed
• [Journal Article] Effects of gonadotrophin treatments on meiotic and developmental competence of oocytes in porcine primordial follicles following xenografting to nude mice. (2006)
  • Author(s): Kaneko H, Kikuchi K, Noguchi J, Ozawa M, Ohnuma K, Maedomari N, Kashiwazaki N.
  • Journal Title: Reproduction 131 Pages: 279-288
  • Peer Reviewed
• [Journal Article] In vitro and in vivo developmental ability of oocytes derived from porcine primordial follicles xenografted into nude mice. (2006)
  • Author(s): Kikuchi K, Kaneko H, Nakai M, Noguchi J, Ozawa M, Ohnuma K, Kashiwazaki N.
  • Journal Title: J Reprod Dev 52 Pages: 51-57
  • NAID: 130000054653
  • Peer Reviewed
• [Journal Article] Effects of gonadotrophin treatments on meiotic and developmental competence of oocytes in porcine primordial follicles following xenografting to nude mice. (2006)
  • Author(s): Kaneko et al.
  • Journal Title: Reproduction 131・2 Pages: 279-288
• [Journal Article] In vitro and in vivo developmental ability of oocytes derived from porcine primordial follicles xenografted into nude mice. (2006)
  • Author(s): Kikuchi et al.
  • Journal Title: Journal of Reproduction and Development 52・1 Pages: 51-57
  • NAID: 130000054653
• [Presentation] 幼若ブタ精巣を移植したヌードマウスの内分泌的特徴と移植精巣の発育 (2007)
  • Author(s): 金子 浩之, ら
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2007-10-20
• [Presentation] 遠心・融合核置換ブタ卵の細胞質量増加による体外受精および胚発生の改善 (2007)
  • Author(s): 前泊 直樹, ら
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2007-10-20
• [Presentation] In vitro embryo production and the related technologies for conservation of porcine genetic resources (2007)
  • Author(s): Kikuchi K., et. al.
  • Organizer: 動物遺伝資源管理韓・日共同Symposium
  • Place of Presentation: 韓国農村振興庁 畜産科学院
  • Year and Date: 2007-10-11
• [Presentation] 幼若ブタ精巣を移植したヌードマウスの内分泌的特徴と移植精巣の発育 (2007)
  • Author(s): 金子浩之、菊地和弘、中井美智子、野口純子
  • Organizer: 第100回日本繁殖生物学会大会
  • Place of Presentation: 東京大学
• [Presentation] 遠心・融合核置換ブタ卵の細胞質量増加による体外受精および胚発生の改善 (2007)
  • Author(s): 前泊直樹、菊地和弘、中井美智子、小沢学、ソムファイタマス、 伊藤潤哉、 野口純子、金子浩之、 永井卓、柏崎直巳
  • Organizer: 第100回日本繁殖生物学会大会
  • Place of Presentation: 東京大学
• [Presentation] Parthenogenetic development of porcine oocytes reconstructed by centrifugation and electrofusion after metaphase-II chromosome transfer. (2007)
  • Author(s): Maedomari N, Kikuchi K, Fahrudin M, Nakai M, Ozawa M, Noguchi J, Kaneko H, Nagai T, Kashiwazaki N.
  • Organizer: 国際胚移植学会(IETS)
  • Place of Presentation: 京都
• [Presentation] 異種間移植を利用した未成熟生殖細胞の成熟と胚発生 (2007)
  • Author(s): 金子浩之、菊地和弘、中井美智子、前泊直樹
  • Organizer: 第9回SSREシンポジウム
  • Place of Presentation: 明治大学
• [Presentation] 遠心・融合による核置換により作出したブタ再構築卵の発生能 (2006)
  • Author(s): 前泊直樹、柏崎直巳、中井美智子、Fahrudin, M、野口純子、金子浩之、菊地和弘
  • Organizer: 第99回日本繁殖生物学会大会
  • Place of Presentation: 名古屋大学
• [Presentation] ヌードマウスへ移植したブタ精巣組織から得られた精子を用いての胚生産 (2006)
  • Author(s): 中井美智子、菊地和弘、柏崎直巳、ソムファイタマス、小沢学、前泊直樹、野口純子、金子浩之
  • Organizer: 第99回日本繁殖生物学会大会
  • Place of Presentation: 名古屋大学
• [Presentation] Porcine blastocysts derived from in vitro matured oocytes injected intra -cytoplasmically with a sperm from testicular tissue xenografted into nude mice. (2006)
  • Author(s): Kikuchi K, Nakai M, Kashiwazaki N, Ozawa M, Noguchi J, Ohnuma K, Kaneko H.
  • Organizer: 国際胚移植学会(IETS)
  • Place of Presentation: オーランド
• [Presentation] Effects of hormone treatments on the developmental competence of porcine primordial follicles following xenografting to nude mice. (2005)
  • Author(s): Kaneko H, Kikuchi K, Noguchi J, Nakai M, Kashiwazaki N, Ozawa M, Ohnuma K, Maedomori N
  • Organizer: 第38回米国繁殖学会(SSR)
  • Place of Presentation: ケベック
• [Presentation] 異種間移植によるブタ原始卵胞卵子からの初期胚発生の試み (2005)
  • Author(s): 金子浩之、菊地和弘、野口純子、小沢学、大沼克彦、前泊直樹
  • Organizer: 第98回日本繁殖生物学会大会
  • Place of Presentation: 静岡大学