STUDY ON THE NERVE-IMMUNE SYSTEMS AND CELLULAR FACTORS INFLUENCE TO THE PATHOGENICTTY AND DISTRIBUTION OF PRP^<Sc>IN ANIMAL PRION DISEASES
| Project/Area Number |
17380180
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Gifu University |
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Principal Investigator |
ISHIGURO Naotaka Gifu University, FACULTY OF APPLIED BIOLOGICAL SCIENCES, PROFESSOR (00109521)
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| Co-Investigator(Kenkyū-buntansha) |
YANAI Takuma GIFU UNIVERSITY, FACULTY OF APPLIED BIOLOGICAL SCIENCES, PROFESSOR (10242744)
KUWATA Kazuo GIFU UNIVERSITY, CENTER FOR EMERGING INFECTIOUS DISEASES, PROFESSOR (00170142)
HORIUCHI Motohiro HOKKAIDO UNIVERSITY, GRADUATE SCHOOL OF VETERINARY MEDICINE, PROFESSOR (30219216)
丸尾 幸嗣 岐阜大学, 応用生物科学部, 教授 (40124276)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥14,170,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥870,000)
Fiscal Year 2007: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2006: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2005: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | Prion / Immunology / Nerve / Transport / Mouse / BSE / Scrapie / Ileum / 体内伝播 / 回腸遠位部 |
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| Research Abstract |
The pathogenesis of animal prion diseases is influenced by prion strain, genetic factors, natural immunology and several cellular factors in the affected hosts. In this study, genetic factors, nerve-immune system and uptake of PrP^<Sc> by macrophages were mainly studied. The following results were obtained.
1. Polymorpbisms of PrP gene: Polymorphisms of the 863 cattle, 118 caprine and 136 sika dear (Cervus Nippon) PrP genes in Japan were examined. Several single-nucleotide polymorphisms (SNPs) were found in cattle and sika dear, but not find any amino acid substitutions. Two polymorphisms associated with the resistance to scrapie were found in 118 caprine.
2. Bovine and sheep nerve systems: To determine the location, namber and segmental distribution of sympathetic, parasympathetic and sensory neurons innervating the ileum in cattle and sheep, tracer experiments with horseradish peroxidase were conducted. The labeled neurons were detected in the celiac and cranial mesenteric ganglion complex in both cattle and sheep. Only a few labeled neurons existed in the caudal mesenteric ganglion and the paravertebral ganglia.
3. Uptake PrP^<Sc> by immune cells: To clarify the uptake the PrP^<Sc> by immune cells, the involvement of bovine macrophage in the early stage of prion infection was studied. Brain homogenates of scrapie and BSE were degraded sequentially in bovine macrophage and bovine peripheral immune cells.
A part of this research has been published in the papers, the other parts are now prepared in the papers.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Sequence variation of bovine prion protein gene in Japanese cattle (Holstein and Japanese Black)2006
Author(s)
Satoshi, Nakamitsu, Takayuki, Miyazawa, Motohiro, Horiuchi, Sadao, Onoe, Yasunori, Ohoba, Hitoshi, Kitagawa, Naotaka, Ishiguro
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Journal Title
The Journal of Veterinary Medical Science 68(1)
Pages: 27-33
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Inhibition of PrP^<Sc> formation by synthetic O-sulfated glycopyranosides and their polymers
Author(s)
Satoko, Yamaguchi, Yoshihiro, Nishida, Kenji, Sasaki, Mikie, Kambara, Chan-Lan, Kim, Naotaka, Ishiguro, Takehiro, Nagatsuka, Hirotaka, Uzawa, Motohiro, Horiuchi
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Journal Title
Biochemical and Biophysical Research Communications 349
Pages: 485-491
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Frequencies of PrP genotypes in meat breeds of Japnese sheep and trail of selective breeding in experimental sheep flock
Author(s)
Jiro, Ohara, Tetsuro, Togari, Aya, Kurokawa, Junko, Maeda, Naotaka, Ishiguro, Hidefumi, Furuoka, Motohiro, Horiuchi
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Journal Title
The Journal of Veterinary Medical Science 69(12)
Pages: 1325-1329
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Localization of foreign nerves cells controlling bovine ileum nerves2009
Author(s)
Yasushige, Ohmori, Yasurou, Atoji, Shouichiro, Saito, Hirosi, Ueno, Yasuo, Inoshima, Naotaka, Ishiguro
Organizer
The 142 annual meeting of Japanese Society of Veterinary Science
Place of Presentation
Yamaguchi
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] ウシ回腸での物質の取り込み機構2008
Author(s)
石黒直隆, ら
Organizer
第145回日本獣医学会学術集会
Place of Presentation
東京 麻布大学
Year and Date
2008-03-28
Description
「研究成果報告書概要(和文)」より
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[Presentation] Nerves control in intestine : comparative study of distribution of nerve system between bovine and sheep2007
Author(s)
Yasushige, Ohmori, Yasuro, Atoji, Shouichiro, Saito, Hiroshi, Ueno, Yasuo, Inoshima, Naotaka, Ishiguro
Organizer
Prion Symposium in 2006
Place of Presentation
Niigata
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Uptake system in bovine ileum considering prion infections2007
Author(s)
Lwine, Sein, Yukiko, Sassa, Naotaka, Ishiguro, Yasuo, Inoshima, Hiroshi, Ueno, Yasushige Ohmori
Organizer
Prion Symposium in 2006
Place of Presentation
Niigata
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Comparative nerve systems among bovine ileum and duodenum, and Sheep ileum2007
Author(s)
Yasushige, Ohmori, Yasuro, Atoji, Shouichiro, Saito, Hiroshi, Ueno, Yasuo, Inoshima, Naotaka, Ishiguro
Organizer
The 144 annual meeting of Japanese Society of Veterinary Science
Place of Presentation
Ebetsu
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Immunohistochemistry of PrP^<c> distribution in bovine digestion tube2007
Author(s)
Nobuo Kitamura, Toru, Izawa, Motoki, Sasaki, Hidefumi, Furuoka, Kiyoshi, Okachi, Daisuke, Hayakawa, Naotaka, Ishiguro
Organizer
The 144 annual meeting of Japanese Society of Veterinary Science
Place of Presentation
Ebetsu
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Uptake and degradation, of PrP^<Sc>by immune cells isolated from cattle and mouse2006
Author(s)
Yukiko, Sassa, Shino, Ito, Michi, Ito, Yasuo, Inoshima, Naotaka, Ishiguro
Organizer
Prion Symposium in 2006
Place of Presentation
Iwate
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Localization of foreign nerve cells controlling bovine ileum nerves2006
Author(s)
Yasushige, Ohmori, Yasurou, Atoji, Shouichiro, Saito, Hiroshi, Ueno, Yasuo, Inoshima, Naotaka, Ishiguro
Organizer
Prion Symposium in 2006
Place of Presentation
Iwate
Description
「研究成果報告書概要(欧文)」より
Related Report
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