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Study on Residual Infectivity of Highly Pathogenic Avian Influenza Virus in Food Products

Research Project

Project/Area Number 17380183
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionTottori University

Principal Investigator

ITO Toshihiro  Tottori University, Faculty of Agriculture, Professor (00176348)

Co-Investigator(Kenkyū-buntansha) OTSUKI Koichi  Kyoto Sangyo University, Faculty of Engineering, Professor (00032293)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,590,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥990,000)
Fiscal Year 2007: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2006: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥8,900,000 (Direct Cost: ¥8,900,000)
Keywordshighly pathogenic avian influenza / chicken meat / chicken egg / temperature / infectivity / disinfectant / H5N1 / virus / ワクチン / 鳥インフルエンザ
Research Abstract

In 2007, the outbreaks of highly pathogenic avian influenza virus subtype H5N1 were occurred in Miyazaki and Okayama prefectures At each of the four farms where cases occurred, the outbreak was quickly discovered and reported, and rapid epidemic prevention measures such as culling, burial, and incineration were successful. The outbreaks were kept to a minimum, and no reoccurrences were confirmed. Therefore, the spreading of harmful rumors on food safety was very small. However, we do not know yet how much virus remains in infected chicken meat and eggs, and what kind of processing can be completely removed it, when highly pathogenic avian influenza occurred.
Therefore, in this study, we examined (1)the effect of some disinfectants on avian influenza virus, (2)the survival period of avian influenza virus in lake water, and (3)quantity of avian influenza virus in infected chicken eggs.
As a result,
(1) Following just 10 seconds exposure to 70% ethanol and 70% isopropanol, less than 10 minutes exposure to 0.1% benzalkonium chloride, 0.5% chlorhexidine gluconate and 500 times dilution, no residual infectivity was noted, suggesting usefulness of these disinfectants.
(2) The infectivity of avian influenza virus in lake water at 4C and 20C was tested and no large fall of the infectivity was observed until 30th day, suggesting the stability of this virus in natural condition.
(3) The virus was not detected in an egg of the death day before of infected chickens, although one of two eggs just before the death contained a virus of 2.5 log EID 50/ml. It was suggested that the possibility is very low, that humans having contaminated eggs will be infected with highly pathogenic avian influenza virus.
Results obtained in this study offer useful information for an anti-avian influenza measure in food industry and general family as well as poultry industry.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (18 results)

All 2007 2006 2005

All Journal Article (16 results) (of which Peer Reviewed: 5 results) Presentation (2 results)

  • [Journal Article] Strong antiviral activity of heated and hydrated dolomite-preliminary investigation.2007

    • Author(s)
      Yamana H, Ito H, Ito T, Murase T, Motoike K, Wakabayashi K, Otsuki K.
    • Journal Title

      J. Vet. Med. Sci 69

      Pages: 217-219

    • NAID

      110006201243

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The quail and chicken intestine have sialyl-galactose sugar chains responsible for the hinding of influenza A viruses to human type receptors2007

    • Author(s)
      Guo CT, Takahashi N, Yagi H, Kato K, Takahashi T, Yi SQ, Chen Y, Ito T, Otsuki K, Kida H, Kawaoka Y, Hidari KI, Miyamoto D, Suzuki T, Suzuki Y
    • Journal Title

      Glycobiology 17

      Pages: 713-724

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Adaptation of an H7N7 equine influenza A virus in mice.2007

    • Author(s)
      Shinya K, Watanabe S, Ito T, Kasai N, Kawaoka Y.
    • Journal Title

      J. Gen. Virol. 88

      Pages: 547-553

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Strong antiviral activity of heated and hydrated dolomite-preliminary investigation2007

    • Author(s)
      Yamana, H, Ito, H, Ito, T, Murase, T, Motoike, K, Wakabayashi, K, Otsuki, K
    • Journal Title

      J. Vet. Med. Sci 69

      Pages: 217-219

    • NAID

      110006201243

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The quail and chicken intestine have sialyl-galactose sugar chains responsible for the binding of influenza A viruses to human type receptors2007

    • Author(s)
      Guo, CT, Takahashi, N, Yagi, H, Kato, K, Takahashi, T, Yi, SQ, Chen, Y, Ito, T, Otsuki, K, Kida, H, Kawaoka, Y, Hidari, KI, Miyamoto, D, Suzuki, T, Suzuki, Y
    • Journal Title

      Glycobiology 17

      Pages: 713-724

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Adaptation of an H7N7 equine influenza A virus in mice2007

    • Author(s)
      Shinya, K, Watanabe, S, Ito, T, Kasai, N, Kawaoka, Y
    • Journal Title

      J. Gen. Virol 88

      Pages: 547-553

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The quail and chicken intestine have sialyl-galactose sugar chains responsible for the binding of influenza A viruses to human type receptors2007

    • Author(s)
      Guo CT, Takahashi N, Yagi H, Kato K, Takahashi T, Yi SQ, Chen Y, Ito T, Otsuki K, Kida H, Kawaoka Y, Hidari
    • Journal Title

      Glycobiology 17

      Pages: 713-724

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adaptation of an H7N7 equine influenza A virus in mice2007

    • Author(s)
      Shinya K, Watanabe S, Ito T, Kasai N, Kawaoka Y.
    • Journal Title

      J. Gen. Virol. 88

      Pages: 547-553

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adaptation of an H7N7 equine influenza A virus in mice.2007

    • Author(s)
      Shinya, K., Watanabe, S., Ito, T., Kasai, N., Kawaoka, Y.
    • Journal Title

      J. Gen. Virol. 88

      Pages: 547-553

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Strong Antiviral Activity of Heated and Hydrated Dolomite Preliminary Investigation.2007

    • Author(s)
      Yamana, H., Ito, H., Ito, T., Murase, T., Motoike, K., Miyoshi, N., Wakabayashi, K.et al.
    • Journal Title

      J. Vet. Med. Sci (印刷中)

    • NAID

      110006201243

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The quail and chicken intestine have sialy-Gal sugar chains responsible for the binding of influenza A viruses to human type receptors.2007

    • Author(s)
      Guo, C.-T., Takahashi, N., Yagi, H., Kato, K., Takahashi, T., Yi, S.O., Chen, Y., Ito, T.et al.
    • Journal Title

      Glycobiology (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The development and characterization of H5 influenza virus vaccines derived from a 2003 human isolate.2006

    • Author(s)
      Horimoto, T., Takada, A., Fujii K., Goto, H.et al.
    • Journal Title

      Vaccine 24

      Pages: 3669-3676

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Human trachea primary epithelial cells express both sialyl-2-3-Gal receptor for human parainfluenza virus type 1 and avian influenza viruses, and sialyl-2-6-Gal receptor for human influenza viruses.2006

    • Author(s)
      Kogure, T., Suzuki, T., Takahashi, T., et al.
    • Journal Title

      Glycoconjugate J. 23

      Pages: 99-104

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Outbreak of highly pathogenic avian influenza in Japan and anti-influenza virus activity of povidone-iodine products.2006

    • Author(s)
      Ito H., Ito T., Hikida, M., Yashiro, J., Otsuka, A.et al.
    • Journal Title

      Delmatology 212

      Pages: 109-112

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Neurovirulence of H7N7 influenza A virus : Brain stem encephalitis accompanied with aspiration pneumonia in mice.2005

    • Author(s)
      Shinya, K., Sutou, A., Kawakami, M., Sakamoto, H.et al.
    • Journal Title

      Arch.Virol. 150

      Pages: 1653-1660

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Outbreak and successful control of highly pathogenic avian influenza in Japan in 2004 caused by an H5N1 subtype.2005

    • Author(s)
      Nishiguchi, A., Yamamoto, T., Tsutsui, T.et al.
    • Journal Title

      Scientific and Technical Review of the OIE 24

      Pages: 933-944

    • Related Report
      2005 Annual Research Report
  • [Presentation] 野鳥によるH5N1ウイルスの伝播について2007

    • Author(s)
      伊藤壽啓
    • Organizer
      第143回日本獣医学会学術集会緊急シンポジウム「新たな高病原性鳥インフルエンザの発生をうけて」
    • Place of Presentation
      つくば市
    • Year and Date
      2007-04-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] H5N1 virus transmission by wild birds2007

    • Author(s)
      Toshihiro, Ito
    • Organizer
      The 143th of Meeting of the Japanese Society of Veterinary Science
    • Place of Presentation
      Tsukuba
    • Year and Date
      2007-04-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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