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Development of a new viral vector that facilitate the functional genomics of legume species including soybean

Research Project

Project/Area Number 17380197
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionHokkaido University

Principal Investigator

MASUTA Chikara  Hokkaido University, Res.Faculty of Agr, Professor (60281854)

Co-Investigator(Kenkyū-buntansha) ABE Jun  Hokkiado University, Res.Faculty of Agr, Associate Prof. (00192998)
KANAZAWA Akira  Hokkiado University, Res.Faculty of Agr, Associate Prof. (30281794)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥15,640,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2006: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2005: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsVirus / Soybean / Vector / Functional analysis / ウイルスベクター / ファンクショナルゲノミクス / ジーンサイレンシング / ファンクショナルゲノミックス
Research Abstract

We developed the Cucumber mosaic virus (CMV-Sj) vector that can infect soybean and is useful for the functional analysis of soybean genes. Because it is still difficult to make transgenic soybean plants, we need to develop a new system by which virus-induced gene silencing (VIGS) is operated to speculate the function of the target gene. In the first year, we created two infectious clones of CMV-Sj (Sj-D and Sj-C) and used them to make the pseudorecombinant viruses that can infect many soybean varieties. In the second and third years, to prove that our technique is applicable to the soybean genes whose functions are unknown, we selected two genes, the chalcone synthase (CHS) gene, the key gene of the anthocyanin biogenesis and the F3'H gene, the gene involved in the accumulation of a metabolite, quercetin. In the result, the CMV vector successfully induced VIGS against both CHS and F3'H, resulting in the phenotypic changes; the color of the seed coat became yellow from brown in the CHS-silenced plants and the quercetin content in the plant was reduced in the F3'H-silenced plants. These results thus suggest that our system is very useful for the soybean functional genomics.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (99 results)

All 2008 2007 2006 2005

All Journal Article (37 results) (of which Peer Reviewed: 16 results) Presentation (56 results) Book (3 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Veinal necrosis induced by Turnip mosaic virus infection in Arabidopsis is a form of defense response accompanying HR-like cell death.2008

    • Author(s)
      B. Kim
    • Journal Title

      Molcular Plant Microbe Interactions 21

      Pages: 260-268

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Degree of RNA silencing andabilityof a viral suppressor vary depending on the cell species ina protoplast system.2008

    • Author(s)
      H. Shimura
    • Journal Title

      Journal of General Plant Pathology (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Down-regulation of flavonoid 3'-hydroxylase gene expression by virus-induced gene silencing in soybean reveals the presence of a threshold mRNA level associated with pigmentation in pubescence2008

    • Author(s)
      A. Nagamatsu
    • Journal Title

      Journal of Plant Physiology (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Veinal necrosis induced by Turnip mosaic virus infection in Arabidopsis is a form of defense response accompanying HR-like cell death.2008

    • Author(s)
      Kim , et. al.
    • Journal Title

      Molcular Plant Microbe Interactions 21

      Pages: 260-268

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Degree of RNA silencing and ability of a viral suppressor vary depending on the cell species in a protoplast system.2008

    • Author(s)
      Shimura , et. al.
    • Journal Title

      Journal of General Plant Pathology (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Down-regulation of flavonoid 3'-hydroxylase gene expression by virus-induced gene silencing in soybean reveals the presence of a threshold mRNA level associated with pigmentation in pubescence.2008

    • Author(s)
      Nagamatsu , et. al.
    • Journal Title

      Journal of Plant Physiology (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Degree of RNA silencing andabilityof a viral suppressor vary depending on the cell species ina protoplast system. Journal of General Plant Pathology2008

    • Author(s)
      H. Shimura
    • Journal Title

      Journal of General Plant Pathology (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Cucumber mosaic virus as a vector modifiable for different host species to produce therapeutic proteins2007

    • Author(s)
      K. Matsuo
    • Journal Title

      Planta 225

      Pages: 277-286

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Comparative serial analysis of gene expression (SAGE) of transcript profiles of tomato roots infected with cyst nematode2007

    • Author(s)
      T. Uehara
    • Journal Title

      Plant Molecular Biology 63

      Pages: 185-194

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Infection of Cucumber mosaic virus to soybean as determined by viral movementprotein.2007

    • Author(s)
      J.S. Hong
    • Journal Title

      Archives of Virology 152

      Pages: 321-328

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Charactrization of silencing suppressor 2b of Cucumber mosaic virus based on examination of its small RNA-binding abilities2007

    • Author(s)
      K. Goto
    • Journal Title

      Plant and Cell Physiology 48

      Pages: 1050-1060

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing.2007

    • Author(s)
      A. Nagamatsu
    • Journal Title

      Plant Biotechnology Journal 5

      Pages: 778-790

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 総説 ジーンサイレンシングによる植物のウイルス抵抗性2007

    • Author(s)
      増田 税
    • Journal Title

      BRAINテクノニュース 121

      Pages: 14-17

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] 総説 植物のウイルスに対する防御機構2007

    • Author(s)
      増田 税
    • Journal Title

      蛋白質核酸酵素

      Pages: 1221-1226

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] Development of Cucumber mosaic virus as a vector modifiable for different host species to produce therapeutic proteins.2007

    • Author(s)
      Matsuo , et. al.
    • Journal Title

      Planta 225

      Pages: 277-286

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Comparative serial analysis of gene expression (SAGE) of transcript profiles of tomato roots infected with cyst nematode.2007

    • Author(s)
      Uehara , et. al.
    • Journal Title

      Plant Molecular Biology 63

      Pages: 185-194

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Infection of Cucumber mosaic virus to soybean as determined by viral movement protein.2007

    • Author(s)
      Hong , et. al.
    • Journal Title

      Archives of Virology 152

      Pages: 321-328

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Charactrization of silencing suppressor 2b of Cucumber mosaic virus based on examination of its small RNA-binding abilities.2007

    • Author(s)
      Goto , et. al.
    • Journal Title

      Plant Cell Physiology 48

      Pages: 1050-1060

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A. Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing.2007

    • Author(s)
      Nagamatsu , et. al.
    • Journal Title

      Plant Biotechnology Journal 5

      Pages: 778-790

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Resistance against plant viruses by gene silencing.2007

    • Author(s)
      Masuta C. Review
    • Journal Title

      Brain Techno News 121

      Pages: 14-17

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Resistance mechanism against plant viruses.2007

    • Author(s)
      Masuta C. Review
    • Journal Title

      PNE

      Pages: 1221-1226

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Pathogenesity of microbes and resistant response of plant.2007

    • Author(s)
      Masuta, C. (edited by Uyeda I.)
    • Journal Title

      Hokkaido University Press, Sapporo

      Pages: 213-219

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Comparative serial analysis of gene expression(SAGE)of transcript profiles of tomato roots infected with cyst nematode2007

    • Author(s)
      T. Uehara
    • Journal Title

      Plant Molecular Biology 63

      Pages: 185-194

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Infection of Cucumber mosaic virus to soybean as determined by viral movementprotein.2007

    • Author(s)
      J. S. Hong
    • Journal Title

      Archives of Virology 152

      Pages: 321-328

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Cucumber mosaic virus as a vector modifiable for different host species to produce therapeutic proteins.2007

    • Author(s)
      Matsuo, K., et al.
    • Journal Title

      Planta 225

      Pages: 277-286

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Rapid induction of transcriptional and post-transcriptional gene silencing using a novel Cucumber mosaic virus vector2006

    • Author(s)
      S. Otagaki
    • Journal Title

      Plant Biotechnology 23

      Pages: 259-265

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Rapid induction of transcriptional and post-transcriptional gene silencing using a novel Cucumber mosaic virus vector.2006

    • Author(s)
      Otagaki , et. al.
    • Journal Title

      Plant Biotechnology 23

      Pages: 259-265

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Recombination in plant viruses.2006

    • Author(s)
      Masuta, C. and Suzuki, M.
    • Journal Title

      In Handbooku of Plant Virology Edited by J.A. Khan and J. Dijkstra, Food Products Press (The Haworth Press, Inc.), NY, USA

      Pages: 185-197

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Rapid induction of transcriptional and post-transcriptional gene silencing using a novel Cucumber mosaic virus vector2006

    • Author(s)
      Otagaki, S. et al.
    • Journal Title

      Plant Biotechnology (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Catalog of Micro-Tom tomato responses to common funagal, bacterial and viral pathogens.2005

    • Author(s)
      H. Takahashi
    • Journal Title

      Journal of General Plant Pathology 71

      Pages: 8-22

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Mapping of the sequence domain for systemic infection in edible lily on the viral genome of Cucumber mosaic virus.2005

    • Author(s)
      N. Yamaguchi
    • Journal Title

      Jounal of General Plant Pathology 71

      Pages: 373-376

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Genetic mapping of the compatibility between a lily isolate of Cucumber mosaic virus and a satellite RNA2005

    • Author(s)
      N. Yamaguchi
    • Journal Title

      Journal of General Virology 86

      Pages: 2359-2369

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The star-type color pattern in Petunia hybrida'Red Star'flowers is induced by the sequence-specific degradation of the chalcone synthase RNA2005

    • Author(s)
      M. Koseki
    • Journal Title

      Plant and Cell Physiology 46

      Pages: 1879-1883

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Catalog of Micro-Tom tomato responses to common funagal, and viral pathogens.2005

    • Author(s)
      Takahashi , et. al.
    • Journal Title

      Jounal of General Plant Pathology 71

      Pages: 8-22

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mapping of the sequence domain for systemic infection in edible lily on the viral genome of Cucumber mosaic virus.2005

    • Author(s)
      Yamaguchi , et. al.
    • Journal Title

      Jounal of General Plant Pathology 71

      Pages: 373-376

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Genetic mapping of the compatibility between a lily isolate of Cucumber mosaic virus and a satellite RNA.2005

    • Author(s)
      Yamaguchi , et. al.
    • Journal Title

      Journal of General Virology 86

      Pages: 2359-2369

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The star-type color pattern in Petunia hybrida 'Red Star' flowers is induced by the sequence-specific degradation of the chalcone synthase RNA.2005

    • Author(s)
      Koseki , et. al.
    • Journal Title

      Plant and Cell Physiology 46

      Pages: 1879-1883

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] RNA-directed DNA methylationのトリガーとなるプロモーター領域の長さと外来遺伝子の効率的な転写不活性化誘導の関連性2008

    • Author(s)
      太田垣駿吾
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌コンベンションセンター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Virus-induced gene silencingを利用したダイズ種子におけるフラボノイド成分の量的改変2008

    • Author(s)
      永松 敦
    • Organizer
      日本育種学会第113回講演会
    • Place of Presentation
      川崎市明治大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Link between the length of the transgene promoter sequence as an inducer for RNA-directed DNA methylation and inactivation of transcription.2008

    • Author(s)
      Otagaki , et. al.
    • Organizer
      Japanese Society of Plant Physiology annual meeting
    • Place of Presentation
      Sapporo Convention Center
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Alteration in the level of a flavonoid in soybean seed by virus-induced gene silencing2008

    • Author(s)
      Nagamatsu , et. al.
    • Organizer
      Japanese Society of Breeding 113th meeting
    • Place of Presentation
      Meiji University
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 植物におけるウイルスベクターを介した外来性プロモーターのメチル化の誘導効率の違いが転写活性に及ぼす影響2007

    • Author(s)
      太田垣駿吾
    • Organizer
      日本分子生物学会第30回年会
    • Place of Presentation
      横浜市、パシフィコ横浜
    • Year and Date
      2007-12-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] Virus-induced gene silencingを利用したダイズにおけるフラボノイドの生合成に関わる遺伝子の機能解析と組成の改変2007

    • Author(s)
      永松 敦
    • Organizer
      日本分子生物学会第30回年会
    • Place of Presentation
      横浜市、パシフィコ横浜
    • Year and Date
      2007-12-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] ダイズにおいてF3' H mRNA蓄積量が毛茸の着色に関与する2007

    • Author(s)
      永松 敦
    • Organizer
      札幌農林学会平成19年度大会
    • Place of Presentation
      札幌市北海道大学
    • Year and Date
      2007-12-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Accumulation of mRNA of the F3'H gene is involved in the color of trichome in soybean.2007

    • Author(s)
      Nagamatsu , et. al.
    • Organizer
      Sapporo Agricultural Society
    • Place of Presentation
      Hokkaido University
    • Year and Date
      2007-12-01
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ダイズにおいてF3'H mRNA蓄積量が毛茸の着色に関与する2007

    • Author(s)
      永松 敦
    • Organizer
      札幌農林学会平成19年度大会
    • Place of Presentation
      札幌市、北海道大学
    • Year and Date
      2007-12-01
    • Related Report
      2007 Annual Research Report
  • [Presentation] キュウリモザイクウイルスサテライトRNAは宿主RNAサイレンシングを促進する2007

    • Author(s)
      小暮 勇樹
    • Organizer
      平成19年度日本植物病理学会北海道部会
    • Place of Presentation
      札幌市北方圏センター
    • Year and Date
      2007-10-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] キュウリモザイクウイルスベクターを用いたペチュニアの内在性遺伝子プロモーターへのメチル化誘導2007

    • Author(s)
      稲場 純一
    • Organizer
      平成19年度日本植物病理学会北海道部会
    • Place of Presentation
      札幌市北方圏センター
    • Year and Date
      2007-10-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] キュウリモザイクウイルスのタバコでの全身移行に及ぼす2bタンパク質の影響2007

    • Author(s)
      辺見 一広
    • Organizer
      平成19年度日本植物病理学会北海道部会
    • Place of Presentation
      札幌市北方圏センター
    • Year and Date
      2007-10-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Satellite RNA of Cucumber mosaic virus can promote RNA silencing of the host plant.2007

    • Author(s)
      Kogure , et. al.
    • Organizer
      Japanese Society of Plant Pathology annual meeting
    • Place of Presentation
      Northern Regions Center, Sapporo
    • Year and Date
      2007-10-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Methylation induction on the promoter of an endogenous gene in petunia by Cucumber mosaic virus vector.2007

    • Author(s)
      Inaba , et. al.
    • Organizer
      Japanese Society of Plant Pathology annual meeting
    • Place of Presentation
      Northern Regions Center, Sapporo
    • Year and Date
      2007-10-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of the 2b protein on the systemic movement of Cucumber mosaic virus in tobacco2007

    • Author(s)
      Henmi , et. al.
    • Organizer
      Japanese Society of Plant Pathology annual meeting
    • Place of Presentation
      Northern Regions Center, Sapporo
    • Year and Date
      2007-10-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] キュウリモザイクウイルスサテライトRNAは宿主RNAサイレンシングを促進する2007

    • Author(s)
      小暮 勇樹
    • Organizer
      平成19年度日本植物病理学会北海道部会
    • Place of Presentation
      札幌市、北方圏センター
    • Year and Date
      2007-10-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] キュウリモザイクウイルスベクターを用いたぺチュニアの内在性遺伝子プロモーターへのメチル化誘導2007

    • Author(s)
      稲場 純一
    • Organizer
      平成19年度日本植物病理学会北海道部会
    • Place of Presentation
      札幌市、北方圏センター
    • Year and Date
      2007-10-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] キュウリモザイクウイルスのタバコでの全身移行に及ぼす2bタンパク質の影響2007

    • Author(s)
      辺見 一広
    • Organizer
      平成19年度日本植物病理学会北海道部会
    • Place of Presentation
      札幌市、北方圏センター
    • Year and Date
      2007-10-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] RNAを介してCaMV 35Sプロモーターに誘導したシトシンのメチル化の動態と転写効率の関連性2007

    • Author(s)
      太田垣駿吾
    • Organizer
      日本育種学会第111回講演会
    • Place of Presentation
      水戸市茨城大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Virus-induced gene silencingを利用したダイズフラボノイド生合成の改変2007

    • Author(s)
      永松 敦
    • Organizer
      日本育種学会第112回講演会
    • Place of Presentation
      鶴岡市山形大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 植物におけるウイルスベクターを介した外来性プロモーターのメチル化の誘導効率の違いが転写活性に及ぼす影響2007

    • Author(s)
      太田垣駿吾
    • Organizer
      日本分子生物学会第30回年会
    • Place of Presentation
      横浜パシフィコ横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Virus-induced gene silencingを利用したダイズにおけるフラボノイドの生合成に関わる遺伝子の機能解析と組成の改変2007

    • Author(s)
      永松 敦
    • Organizer
      日本分子生物学会第30回年会
    • Place of Presentation
      横浜パシフィコ横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Link between the transcription efficiency of cytocine methylation on the 35S promoter induced by RNA.2007

    • Author(s)
      Otagaki , et. al.
    • Organizer
      Japanese Society of Breeding 111th meeting
    • Place of Presentation
      Ibaragi University
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Alteration in the flavonoid content in soybean by virus-induced gene silencing.2007

    • Author(s)
      Nagamatsu , et. al.
    • Organizer
      Japanese Society of Breeding 112th meeting
    • Place of Presentation
      Yamagata University
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of the methylation status on the transgene promoter induced by a viral vector on the transcription activity.2007

    • Author(s)
      Otagaki , et. al.
    • Organizer
      The Molecular Biology Society of Japan 30th meeting
    • Place of Presentation
      Pacifico Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Functional analysis of the gene involved in the flavonoid biosynthesis and alteration of its content by virus-induced gene silencing.2007

    • Author(s)
      Nagamatsu , et. al.
    • Organizer
      The Molecular Biology Society of Japan 30th meeting
    • Place of Presentation
      Pacifico Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] RNAを介してCaMV 35Sプロモーターに誘導したシトシンのメチル化の動態と転写効率の関連性2007

    • Author(s)
      太田垣駿吾
    • Organizer
      日本育種学会第111回講演会
    • Place of Presentation
      水戸市、茨城大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] Virus-induced gene silencingを利用したダイズフラボノイド生合成の改変2007

    • Author(s)
      永松 敦
    • Organizer
      日本育種学会第112回講演会
    • Place of Presentation
      鶴岡市、山形大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] 植物における外来性プロモーター領域のメチル化状態と転写型ジーンサイレンシングの安定性2006

    • Author(s)
      太田垣駿吾
    • Organizer
      札幌農林学会平成18年度大会
    • Place of Presentation
      札幌市北海道大学
    • Year and Date
      2006-12-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Methylation status on the transgene promoter and stability of transcriptional gene silencing.2006

    • Author(s)
      Otagaki , et. al.
    • Organizer
      Sapporo Agricultural Society
    • Place of Presentation
      Hokkaido University
    • Year and Date
      2006-12-02
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] RNAサイレンシングのサプレッサータンパク質の大腸菌発現2006

    • Author(s)
      目黒文乃
    • Organizer
      平成18年度日本植物病理学会北海道部会
    • Place of Presentation
      札幌市北海道大学
    • Year and Date
      2006-10-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] プロトプラストで誘導されるRNAサイレンシングに及ぼす植物ウイルスサプレッサーの影響2006

    • Author(s)
      志村華子
    • Organizer
      平成18年度日本植物病理学会北海道部会
    • Place of Presentation
      札幌市北海道大学
    • Year and Date
      2006-10-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] キュウリモザイクウイルスのsiRNA蓄積に対する2bタンパク質の影響2006

    • Author(s)
      小暮勇樹
    • Organizer
      平成18年度日本植物病理学会北海道部会
    • Place of Presentation
      札幌市北海道大学
    • Year and Date
      2006-10-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] E. coli expression of RNA silencing suppressor proteins.2006

    • Author(s)
      Meguro , et. al.
    • Organizer
      Japanese Society of Plant Pathology annual meeting
    • Place of Presentation
      Hokkaido University
    • Year and Date
      2006-10-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of viral silencing suppressor on RNA silencing in protoplasts.2006

    • Author(s)
      Shimura , et. al.
    • Organizer
      Japanese Society of Plant Pathology annual meeting
    • Place of Presentation
      Hokkaido University
    • Year and Date
      2006-10-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of the 2b protein on the accumulation of siRNAs of Cucumber mosaic virus.2006

    • Author(s)
      Kogure , et. al.
    • Organizer
      Japanese Society of Plant Pathology annual meeting
    • Place of Presentation
      Hokkaido University
    • Year and Date
      2006-10-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] キュウリモザイクウイルスに由来する新規ウイルスベクターによる高効率の転写型および転写後型ジーンサイレンシングの誘導2006

    • Author(s)
      太田垣駿吾
    • Organizer
      日本育種学会第109回講演会
    • Place of Presentation
      東京都東京農工大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] RNA-directed DNA methylationのトリガーとなるプロモーター領域の長さは外来性遺伝子の効率的な転写不活性化に影響を与える2006

    • Author(s)
      太田垣駿吾
    • Organizer
      日本育種学会第110回講演会
    • Place of Presentation
      松山市愛媛大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Efficient induction of transcriptional and post-transcriptional gene silencing by a novel viral vector based on Cucumber mosaic virus.2006

    • Author(s)
      Otagaki , et. al.
    • Organizer
      Japanese Society of Breeding 109th meeting
    • Place of Presentation
      Tokyo University of Agriculture and Technology
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The length of the traqnsgene promoter sequence as an inducer for RNA-directed DNA methylation affects gene silencing.2006

    • Author(s)
      Otagaki , et. al.
    • Organizer
      Japanese Society of Breeding 110th meeting
    • Place of Presentation
      Ehime University
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] RNA-directed DNA methylationによる外来遺伝子の転写不活性化の誘導および維持には標的となるプロモーターの部位が重要である2005

    • Author(s)
      太田垣駿吾
    • Organizer
      札幌農林学会平成17年度大会
    • Place of Presentation
      札幌市北海道大学
    • Year and Date
      2005-12-03
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The target promoter position of the transgene is important for the induction and maintenance of RNA-directed DNA methylation.2005

    • Author(s)
      Otagaki , et. al.
    • Organizer
      Sapporo Agricultural Society
    • Place of Presentation
      Hokkaido University
    • Year and Date
      2005-12-03
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The star-type color patterns in flowers of transgenic and non-transgenic petunia plants are induced by the sequence-specific degradation of the chalcone synthase RNA.2005

    • Author(s)
      Koseki, M.
    • Organizer
      10th International Congress of SABRAO
    • Place of Presentation
      Tsukuba International Congress center, Tsukuba
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Induction of RNA-directed DNA methylation and stable transcriptional silencing of a transgene using a novel plant viral vector2005

    • Author(s)
      Otagaki, S.
    • Organizer
      10th International Congress of SABRAO
    • Place of Presentation
      Tsukuba International Congress center, Tsukuba
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ペチュニア花弁における星型模様の形成には転写後のRNA分解が関与する2005

    • Author(s)
      小関麻衣子
    • Organizer
      日本育種学会第107・108回講演会
    • Place of Presentation
      筑波大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 植物ウイルスベクターによる外来遺伝子の転写不活性化の誘導とその維持に関する解析2005

    • Author(s)
      太田垣駿吾
    • Organizer
      日本育種学会第107・108回講演会
    • Place of Presentation
      筑波大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] カルコーン合成酵素遺伝子RNAの配列特異的な分解がペチュニア花弁における星型模様の形成を誘導する2005

    • Author(s)
      小関麻衣子
    • Organizer
      日本遺伝学会第77回大会
    • Place of Presentation
      東京都オリンピック記念青少年総合センター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] RNA-directed DNA methylationの標的とするプロモーターの部位の違いは後代での転写不活性化の維持に影響を与える2005

    • Author(s)
      太田垣駿吾
    • Organizer
      日本分子生物学会第28回年会
    • Place of Presentation
      福岡市ヤフードーム
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 花の模様の形成を誘導する配列特異的RNA分解2005

    • Author(s)
      小関麻衣子
    • Organizer
      日本分子生物学会第28回年会
    • Place of Presentation
      福岡市ヤフードーム
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The star-type color patterns in flowers of transgenic and non-transgenic petunia plants are induced by the sequence-specific degradation of the chalcone synthase RNA.2005

    • Author(s)
      Koseki , et. al.
    • Organizer
      10^<th> International Congress of SABRAO
    • Place of Presentation
      Tsukuba International Congress center, Tsukuba
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Induction of RNA-directed DNA methylation and stable transcriptional silencing of a transgene using a novel plant viral vector.2005

    • Author(s)
      Otagaki , et. al.
    • Organizer
      10^<th> International Congress of SABRAO
    • Place of Presentation
      Tsukuba International Congress center, Tsukuba
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Post-transcriptional RNA degradation affects the formation of a star pattern in petunia petal.2005

    • Author(s)
      Koseki , et. al.
    • Organizer
      Japanese Society of Breeding 107th and 108th meeting
    • Place of Presentation
      Tsukuba University
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of the induction and maintenance of transcriptional gene silencing of a transgene by a viral vector.2005

    • Author(s)
      Otagaki , et. al.
    • Organizer
      Japanese Society of Breeding 107th and 108th meeting
    • Place of Presentation
      Tsukuba University
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sequence-specific RNA degradation of the chalcone synthase gene induces the formation of a star color pattern in petunia petal.2005

    • Author(s)
      Koseki , et. al.
    • Organizer
      The Genetic Society of Japan 77th Annual Meeting
    • Place of Presentation
      National Olympics Memorial Youth Center
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The target promoter position of the transgene for RNA-directed DNA methylation affects the maintenance of gene silencing in the progeny.2005

    • Author(s)
      Otagaki , et. al.
    • Organizer
      The Molecular Biology Society of Japan 28th meeting
    • Place of Presentation
      Yahoo Dome
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sequence-specific RNA degradation to form flower color patterns.2005

    • Author(s)
      Koseki , et. al.
    • Organizer
      The Molecular Biology Society of Japan 28th meeting
    • Place of Presentation
      Yahoo Dome
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 微生物の病原性と植物の防御応答.2007

    • Author(s)
      増田税(上田一郎編)
    • Publisher
      北海道大学出版会, 札幌.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 微生物の病原性と植物の防御応答.2007

    • Author(s)
      増田税(上田一郎編)
    • Total Pages
      231
    • Publisher
      北海道大学出版会,札幌.
    • Related Report
      2007 Annual Research Report
  • [Book] Recombination plant viruses. In Handbooku of Plant Virology2006

    • Author(s)
      Masuta, C. and Suzuki, M. (Edited by J.A.Khan and J.Dijkstra)
    • Publisher
      Food Products Press (The Haworth Press, Inc.), NY, USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 形質転換されたダイズ及びその製造方法2007

    • Inventor(s)
      金澤章ほか
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2007-050134
    • Filing Date
      2007-02-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 抗ウイルス剤2007

    • Inventor(s)
      増田税ほか
    • Industrial Property Rights Holder
      北海道大学・日本曹達
    • Industrial Property Number
      2007-077970
    • Filing Date
      2007-03-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 植物ウイルスベクター2007

    • Inventor(s)
      増田税, 他
    • Industrial Property Rights Holder
      北海道大学, 他
    • Acquisition Date
      2007-09-14
    • Related Report
      2007 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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