| Project/Area Number |
17380199
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HISATSUNE Tatsuhiro The University of Tokyo, Graduate School of Frontier Sciences, Associate Professor (10238298)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2006: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 2005: ¥8,100,000 (Direct Cost: ¥8,100,000)
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| Keywords | Neural Stem Cells / Learning / GABA / Hippocampus / Adult / Theta rhythm / Calcium / Imaging / ニューロン新生 / 神経回路再生 / 脳梗塞 / 成体(大人) / 霊長類モデル / 記憶 / 成体脳のニューロン新生 |
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| Research Abstract |
Adult neurogenesis occurs in the dentate gyrus of hippocampus. A series of reports have suggested that the process of this neuronal differentiation can be regulated by the hippocampal network activities. New neurons are generated from sequential differentiation from radial-glia-like stem/progenitor cell (type-1 cell) following the generation of transiently amplifying progenitor cell (type-2 cell). In this study, we found that type-2 cell receives GABAergic inputs by means of targeted-patch clump recordings of progenitor cells in a fresh hippocampal slice from nesin-GFP mice, in combination with an immunohistochemical (con-focal and EM) analysis.
Due to the elevated [C1-]i in type-2 cell, the GABAergic activity through GABAA receptor depolarized the hippocampal progenitor. This excitation induced the elevation of[Ca^<2+>]i, and then promoted the expression of NeuroD, a positive regulator for neuronal commitment. A BrdU-pulse labeling study using living animals with the injections of GABAA receptor agonists and antagonists clearly showed that this excitatory GABAergic input on type-2 cell promotes its terminal differentiation toward post-mitotic neurons. Our study demonstrates the activity-dependent neuronal differentiation of type-2 progenitor cells by means of excitatory GABAergic inputs.
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