Studies on Sphingomyelin and Cholesterol as Acute Risk Factors

• Project/Area Number: 17390011
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Kyoto University
• Principal Investigator: HANDA Tetsurou Kyoto University, 薬学研究科, 教授 (00025719)
• Co-Investigator(Kenkyū-buntansha): NAKANO Minoru 京都大学, 薬学研究科, 准教授 (70314226)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,330,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥1,530,000); Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000); Fiscal Year 2005: ¥7,200,000 (Direct Cost: ¥7,200,000)
• Keywords: スフィンゴミエリン / コレステロール / カイロミクロン / 細胞死 / HDL / カイロミクロンレムナント / apoE / リソソーム膜ストレス / apoA-I / areβ-HDL / apoB / 細胞障害 / apoA-1 / HDL形成 / 脂質の移動と交換 / preβ-HDL / エマルション / リポタンパクモデル / セラミド / アポリポタンパクE / アポリポタンパクB / 変性LDL

Research Abstract

スフィンゴミエリン(SM)やコレステロール(Chol)が細胞に蓄積すると細胞死が誘導されるが、これらを高濃度で含有する血漿リポタンパクが過剰にあるときも細胞死が起こることを明らかにし、そのメカニズムを研究した。また、これら脂質を細胞より排出するHDL粒子の生成を生物物理学的に調査した。

Research Products

• [Journal Article] Static and Dynamic Properties of Phospholipid Bilayer Nanodiscs (2009)
  • Author(s): M. Nakano,M. Fukuda, T. Kudo, M. Miyazaki, Y. Wada,N. Matsuzaki, H. Endo, T. HandaJ. Am
  • Journal Title: Chem. Soc (in press)
  • Peer Reviewed
• [Journal Article] Flip-Flop of Phospholipids in Vesicles: Kinetic Analysis with Time-Resolved Small-Angle Neutron Scattering (2009)
  • Author(s): M. Nakano, M. Fukuda, T. Kudo, N. Matsuzaki, T. Azuma, K. Sekine, H. Endo, T. Handa
  • Journal Title: J. Phys. Chem B 113 (19) Pages: 6745-6748
  • Peer Reviewed
• [Journal Article] Flip-Flop of Phospholipids in Vesicles : Kinetic Analysis with Time-Resolved Small-Angle Neutron Scattering. (2009)
  • Author(s): M. Nakano, M. Fukuda, I. Kudo, N. Matsuzaki, T. Azuma, K. Sekine, H. Endo, T. Handa
  • Journal Title: J. Phys. Chem. B (in press)
  • Peer Reviewed
• [Journal Article] Useful Modified Cellulose Polymers as New Emulsifiers of Cubosomes. (2009)
  • Author(s): M. Uyama, M. Nakano, J. Yamashita, T. Handa
  • Journal Title: Langmuir (in press)
  • Peer Reviewed
• [Journal Article] Synthetic Pentapeptides Inhibiting Autophosphorylation of Insulin Receptor in a Non-ATP-Competitive Mechanism. (2009)
  • Author(s): M. Kato, M. Abe, Y. Kuroda, M. Hirose, M. Nakano, T. Handa
  • Journal Title: J. Pept. Sci. 15(5) Pages: 327-336
  • Peer Reviewed
• [Journal Article] Static and Dynamic Properties of Phospholipid Bilayer Nanodiscs. (2009)
  • Author(s): M. Nakano, M. Fukuda, T. Kudo, M. Miyazaki, Y. Wada, N. Matsuzaki, H. Endo, T. Handa
  • Journal Title: J. Am. Chem. Soc. (in press)
  • Peer Reviewed
• [Journal Article] Role of the N- and C-terminal Domains in Binding of Apolipoprotein E Isoforms to Heparan Sulfate and Dermatan Sulfate: A Surface Plasmon Resonance Study (2008)
  • Author(s): Y. Yamauchi, N. Deguchi, C. Takagi, M. Tanaka, P. Dhanasekaran, M. Nakano, T. HandaC. Phillips, S. Lund-Katz, H. Saito, M
  • Journal Title: Biochemistry 47 (25) Pages: 6702-6710
  • Peer Reviewed
• [Journal Article] Conformational Change of Apolipoprotein A-I and HDL Formation from Model Membranes under Intracellular Acidic Conditions (2008)
  • Author(s): M. Fukuda, M. Nakano, M. Miyazaki, M. Tanaka, H. Saito, S.Kobayashi, M. Ueno, T. Handa, J
  • Journal Title: Lipid Res 49 (11) Pages: 2419-2426
  • Peer Reviewed
• [Journal Article] Cytotoxicity of lipid-free apolipoprotein B (2008)
  • Author(s): S. Morita, Y. Deharu, E. Takata, M. Nakano, T. Handa
  • Journal Title: Biochim. Biophys Acta 1778 (11) Pages: 2594-2603
  • Peer Reviewed
• [Journal Article] アポリポタンパク-脂質膜相互作用によるディスク状複合体形成と高密度リポタンパク質形成原理の考察 (2008)
  • Author(s): 中野実, Yakugaku Zasshi
  • Journal Title: 128(5) Pages: 687-693
• [Journal Article] SANS-Uを使った脂質のベシクル間移動の研究 (2008)
  • Author(s): 中野実
  • Journal Title: 波紋 18 (2) Pages: 82-85
• [Journal Article] Inhibition of Autophosphorylation of Epidermal Growth Factor Receptor by a Small Peptide not Employing an ATP-Competitive Mechanism. (2008)
  • Author(s): M. Abe, Y. Kuroda, M. Hirose, M. Kato, M. Murakami, Y. Watanabe, M. Nakano, T. Handa
  • Journal Title: Biopolymers 89(1) Pages: 40-51
  • Peer Reviewed
• [Journal Article] Role of the N- and C-teminal Domains in Binding of Apolipoprotein E Isoforms to Heparan Sulfate and Dermatan Sulfate : A Surface Plasmon Resonance study. (2008)
  • Author(s): Y. Yamauchi, N. Deguchi, C. Takagi, M. Tanaka, P. Dhanasekaran, M. Nakano, T. Handa, M. CPhillips, S. Lund-Katz, H. Saito
  • Journal Title: Biochemistry 47(25) Pages: 6702-6710
  • Peer Reviewed
• [Journal Article] Effect of Polymer Type on the Dissolution Profile of Amorphous Solid Dispersions Containing Felodipine (2008)
  • Author(s): H. Konno, T. Handa, D. E. Alonzo, L. S. Taylor
  • Journal Title: Eur. J. Pharm. Biopharm 70(2) Pages: 493-499
  • Peer Reviewed
• [Journal Article] Confomational Change of Apolipoprotein A-I and HDL Formation from Model Meibranes under Intracellular Acidic Conditions. (2008)
  • Author(s): M. Fukuda, M. Nakano, M. Miyazaki, M. Tanaka, H. Saito, S. Kobayashi, M. Ueno, T. Handa
  • Journal Title: J. Lipid Res 49(11) Pages: 2419-2426
  • Peer Reviewed
• [Journal Article] Cytotoxicity of lipid-free apolipoprotein B (2008)
  • Author(s): Cytotoxicity of lipid-free apolipoprotein B
  • Journal Title: Biochim. Biophys. Acta 1778(11) Pages: 2594-2603
  • Peer Reviewed
• [Journal Article] Spontaneous Reconstitution of Discoidal HDL from Sphingomyelin-Containing Model Membranes by Apolipoprotein A-I (2007)
  • Author(s): M. Fukuda, M. Nakano, S. Sriwongsitanont, M. Ueno, Y. Kuroda, T. Handa
  • Journal Title: J. Lipid Res 48 (4) Pages: 882-889
  • Peer Reviewed
• [Journal Article] Determination of Interbilayer and Transbilayer Lipid Transfers by Time-Resolved Small-Angle Neutron Scattering (2007)
  • Author(s): M. Nakano, M. Fukuda, T. Kudo, H. Endo, T. Handa
  • Journal Title: Phys. Rev. Lett 98 (23),238101 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Bile Salt-Dependent Efflux of Cellular Phospholipids Mediated by ATP Binding Cassette Protein B4 (2007)
  • Author(s): S. Morita, A. Kobayashi, Y. Takanezawa, N. Kioka, T. Handa, H. Arai, M. Matsuo, K. Ueda
  • Journal Title: Hepatology 46 (1) Pages: 188-199
  • Peer Reviewed
• [Journal Article] Novel Action of Apolipoprotein E (ApoE) : ApoE Isoform Specifically Inhibits Lipid-Particle-Mediated Cholesterol Release from Neurons. J-S. Gong (2007)
  • Author(s): S. Morita, M. Kobayashi, T. Handa, S. C. Fujita, K. Yanagisawa. M. Michikawa
  • Journal Title: olecular Neurodegeneration 2, 9 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Interaction of an Amphipathic Peptide with Phosphatidycholine/Phosphatidylethanolamine Mixed Membranes (2007)
  • Author(s): K. Shintou, M. Nakano, T. Kamo, Y. Kuroda, T. Handa,
  • Journal Title: Biophys J. 93 (11) Pages: 3900-3906
  • Peer Reviewed
• [Journal Article] 脂質エンジニアリング : ものづくりプラットフォームとしての脂質膜 (2007)
  • Author(s): 半田哲郎
  • Journal Title: 膜 32(1) Pages: 1-1
  • NAID: 10018696167
• [Journal Article] 自発的HDL様粒子形成における膜構造の影響 (2007)
  • Author(s): 中野実
  • Journal Title: 膜 32(5) Pages: 247-252
  • NAID: 10019834056
• [Journal Article] Spontaneous Reconstitution of Discoidal HDL from Sphingomyelin-Containing Model Membranes by Apolipoprotein A-I. (2007)
  • Author(s): M. Fukuda, M. Nakano, S. Sriwong-sitanont, M. Ueno, Y. Kuroda, T. Handa
  • Journal Title: J. Lipid Res. 48 Pages: 882-889
• [Journal Article] Determination of Interbilayer and Transbilayer Lipid Transfers by Time-Resolved Small-Angle Neutron Scattering. (2007)
  • Author(s): M. Nakano, M. Fukuda, T. Kudo, H. Endo, T. Handa
  • Journal Title: Phys. Rev. Lett. 98
• [Journal Article] Interaction of an Amphipathic Peptide with Phosphatidycholine/Phosphatidylethanolamine Mixed Membranes. (2007)
  • Author(s): K. Shintou, M. Nakano, T. Kamo, Y. Kuroda, T. Handa
  • Journal Title: Biophys. J. 93 Pages: 3900-3806
• [Journal Article] Spontaneous Reconstitution of Discoidal HDL from Sphingomyelin-containing Model Membranes by Apolipoprotein A-I. (2007)
  • Author(s): M.Fukuda, M.Nakano, S.Sriwongsitanont, M.Ueno, Y.Kuroda, T.Handa
  • Journal Title: J. Lipid Res. 48 Pages: 882-889
• [Journal Article] 脂質エンジニアリング : ものづくりプラットフォームとしての脂質膜 (2007)
  • Author(s): 半田 哲郎
  • Journal Title: 膜 32 (1) Pages: 1-1
  • NAID: 10018696167
• [Journal Article] Effects of an Amphipathic α-Helical Peptide on Lateral Pressure and Water Penetration in Phosphatidylcholine and Monoolein Mixed Membranes (2006)
  • Author(s): T. Kamo, M. Nakano, Y. Kuroda, T. Handa
  • Journal Title: J. Phys. Chem B 110 (49) Pages: 24987-24992
  • Peer Reviewed
• [Journal Article] 非二分子膜構造を有する脂質微粒子の創成とその構造評価に関する研究 (2006)
  • Author(s): 中野実
  • Journal Title: 膜 31(4) Pages: 202-206
  • NAID: 10017654278
• [Journal Article] Effects of an amphipathic a-helical peptide on lateral pressure and water penetration in phosphathidylcholine and monoolein mixed membranes (2006)
  • Author(s): T.Kamo, M.Nakano, Y.Kuroda, T.Handa
  • Journal Title: J. Phys. Chem. B 110 Pages: 24987-24992
• [Journal Article] Inhibition of autophosphorylation of epidermal growth factor receptor by small peptides in vitro (2006)
  • Author(s): M.Abe, Y.Kuroda, M.Hirose, Y.Watanabe, M.Nakano, T.Handa
  • Journal Title: Br. J. Pharmacol. 147 (4) Pages: 402-411
• [Journal Article] Two-Step Mechanism of Binding of Apolipoprotein E to Heparin (2005)
  • Author(s): M. Futamura, P. Dhanasekaran, T. Handa, M. C. Phillips, S. Lund-Katz, H. Saito, J. Biol. Chem
  • Journal Title: Implications for the Kinetics of Apolipoprotein E-Heparan Sulfate Proteoglycan Complex Formation on Cell Surfaces. 280(7) Pages: 5414-5422
  • Peer Reviewed
• [Journal Article] Formation of Ceramide-Enriched Domains in Lipid Particles Enhances the Binding of Apolipoprotein E. S. Morita (2005)
  • Author(s): M. Nakano, A. Sakurai, Y. Deharu, A. V-Doi, T. Handa14. Effects of Cholesterol in Chylomicron Remnant Models of Lipid Emulsions on ApoE-Mediated Uptake and Cytotoxicity of Macrophages, A. Sakurai, S. Morita, K. Wakita, Y. Deharu,
  • Journal Title: FEBS Letters 579(7) Pages: 1759-1764
  • Peer Reviewed
• [Journal Article] Effects of Cholesterol in Chylomicron Remnant Models of Lipid Emulsions on ApoE-Mediated Uptake and Cytotoxicity of Macrophages (2005)
  • Author(s): A. Sakurai, S. Morita, K. Wakita, Y. Deharu, M. Nakano, T. Handa,
  • Journal Title: J. Lipid Res 46 (10) Pages: 2214-2220
  • Peer Reviewed
• [Journal Article] Effects of the Core Lipid on the Energetics of Binding of ApoA-I to Model Lipoprotein Particles of Different Sizes (2005)
  • Author(s): M. Tanaka, H. Saito, P. Dhanasekaran, S. Wehrli, T. Handa , S. Lund-Katz, M. C. Phillips
  • Journal Title: Biochemistry 44 (31) Pages: 10689-10695
  • Peer Reviewed
• [Journal Article] 脂質エマルションの表面構造と血漿アポリポタンパクの結合 (2005)
  • Author(s): 半田哲郎
  • Journal Title: 表面 43 Pages: 233-241
  • NAID: 40006987349
• [Journal Article] セラミドと動脈硬化の発生 (2005)
  • Author(s): 森田真也, 半田哲郎
  • Journal Title: 化学 60(10) Pages: 72-73
• [Journal Article] 脂質乳化粒子の構造と機能 (2005)
  • Author(s): 半田哲郎
  • Journal Title: フラグランスジャーナル 19 Pages: 10-16
• [Journal Article] Effects of cholesterol in chylomicron remnant models of lipid emulsions on apoE-mediated uptake and cytotoxity of macrophages (2005)
  • Author(s): A.Sakurai, S.Morita, W.Wakita, Y.Deharu, M.Nakano, T.Handa
  • Journal Title: Journal of Lipid Research 46・10 Pages: 2214-2220
• [Journal Article] Two-srep mechanism of binding of apolipoprotein E to heparin (2005)
  • Author(s): M.Futamura, P.Dhanasekaran, T.Handa, M.C.Phillips, S.Lund-Katz, H.Saito
  • Journal Title: Journal of Biological Chemistry 280・7 Pages: 5414-5422
• [Journal Article] Formation of ceramide-enriched domains in lipid particles enhances the binding of apolipoprotein E (2005)
  • Author(s): S.Motita, M.Nakano, A.Sakurai, Y.Deharu, A.Vetut-Doi, T.Handa
  • Journal Title: FEBS Letter 579・7 Pages: 1759-1764
• [Journal Article] Detection of bilayer packing stress and its release in lamellar-cubic phase transition by time-resolved fluorescence anisotropy (2005)
  • Author(s): M.Nakano, T.Kamo, A.Sugita, T.Handa
  • Journal Title: Journal of Physical Chemistry B 109・10 Pages: 4754-4760
• [Journal Article] 脂質エマルションの表面構造と血漿アポリポタンパクの結合 (2005)
  • Author(s): 半田 哲郎
  • Journal Title: 表面 43・5 Pages: 233-241
  • NAID: 40006987349
• [Journal Article] セラミドと動脈硬化の発生 (2005)
  • Author(s): 森田 真也, 半田 哲郎
  • Journal Title: 化学 60・10 Pages: 72-73
• [Presentation] 時分割中性子散乱法によるリン脂質粒子間移動とフリップフロップの評価 (2009)
  • Author(s): 福田正和, 工藤嵩之, 中野実, 半田哲郎
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-27
• [Presentation] 上皮成長因子受容体と新規活性阻害ペプチドの相互作用様式の検討 (2009)
  • Author(s): 阿部峰夫, 黒田義弘, 廣瀬宗孝, 中野実, 半田哲郎
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-27
• [Presentation] ホスホリパーゼD酵素反応の膜脂質組織依存性の評価 (2009)
  • Author(s): 東拓人, 関根和久, 中野実, 半田哲郎
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-27
• [Presentation] 両親媒性ペプチドのLUV膜への結合性に対する膜組織の影響 (2009)
  • Author(s): 和田祐典, 中野実, 半田哲郎
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-27
• [Presentation] 上皮成長因子受容体を再構成した脂質ナノディスクの調整および新規阻害剤の評価 (2009)
  • Author(s): 山本容輔, 阿部峰夫, 黒田義弘, 中野実, 半田哲郎
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-27
• [Presentation] 時分割中性子散乱による膜脂質のダイナミクスの評価 (2008)
  • Organizer: 中性子科学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-12-02
• [Presentation] 両親媒性ヘリックスのエマルジョンへの結合性に対するコレテロールの影響 (2008)
  • Author(s): 小澤真衣, 中野実, 半田哲郎
  • Organizer: 第29回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2008-11-26
• [Presentation] 中性子散乱法および蛍光法を用いた脂質粒子間移動とフリップフロップの評価 (2008)
  • Author(s): 工藤嵩之, 福田正和, 中野実, 半田哲郎
  • Organizer: 第29回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2008-11-26
• [Presentation] Lipid Transfer Dynamics in Nanoparticle Systems (2008)
  • Author(s): Minoru Nakano
  • Organizer: Julich Soft Matter Days
  • Place of Presentation: Bonn,Germany
  • Year and Date: 2008-11-14
• [Presentation] Evaluation of intervesicular transfer and flip-flop of phospholipids by time-resolved small-angle neutron scattering (2008)
  • Author(s): Minoru Nakano, Takayuki Kudo, Masakazu Fukuda, Tetsurou Handa
  • Organizer: 49th International Conference on the Bioscience of Lipids
  • Place of Presentation: Maastricht, Netherlands
  • Year and Date: 2008-08-28
• [Presentation] リボソームからリポタンパク質モデル粒子へのコレステロール移動速度の評価 (2008)
  • Author(s): 松崎直哉, 福田正和, 中野実, 半田哲郎
  • Organizer: 日本膜学会第30年会
  • Place of Presentation: 東京理科大学・森戸記念館
  • Year and Date: 2008-05-16
• [Presentation] エンドソーム内の酸性条件により促進されるモデル膜のHDL化 (2008)
  • Author(s): 福田正和, 中野実, 半田哲郎
  • Organizer: 日本膜学会第30年会
  • Place of Presentation: 東京理科大学・森戸記念館
  • Year and Date: 2008-05-16
• [Presentation] コレステロールによるエマルションの表面状態と両親媒性ヘリックス結合性の変化 (2008)
  • Author(s): 中野実
  • Organizer: 膜学会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-15
• [Presentation] Evaluation of Intervesicular Transfer and Flip-Flop of Phospholipids by Time-Resolved Small-Angle Neutron Scattering (2008)
  • Author(s): Minoru Nakano
  • Organizer: 49th International Conference of Biochemistry of Lipids
  • Place of Presentation: Maastricht, Netherlands
• [Presentation] In Situ Determination of Interbilayer and Transbilayer Lipid Transfers in Liposomes (2008)
  • Author(s): Minoru Nakano
  • Organizer: 11th Liposome Research Days Conference
  • Place of Presentation: Yokohama
• [Presentation] 中性子散乱を利用した膜脂質のダイナミクス評価 (2007)
  • Author(s): 中野実
  • Organizer: 膜シンポ
  • Place of Presentation: 山口
  • Year and Date: 2007-11-15
• [Presentation] 時分割中性子小角散乱による膜脂質ダイナミクスの評価 (2007)
  • Author(s): 中野実
  • Organizer: コロイド
  • Place of Presentation: 松本
  • Year and Date: 2007-09-22
• [Presentation] Effects of Cholesterol in Chylomicron Remnant Models on ApoE-Mediated Uptake and Cyototoxity of Macrophages (2007)
  • Author(s): Tetsuropu Handa
  • Organizer: 48th ICBL(第48回 国際脂質生化学会議)
  • Place of Presentation: Turku, Finland
  • Year and Date: 2007-09-04
• [Presentation] 自発的HDL様粒子形成における膜構造の影響 (2007)
  • Author(s): 中野実
  • Organizer: 膜学会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-10
• [Presentation] アポリポタンパク-脂質膜相互作用によるディスク状複合体形成とHDL形成原理の考察 (2007)
  • Author(s): 中野実
  • Organizer: 薬学会
  • Place of Presentation: 富山
  • Year and Date: 2007-03-29
• [Presentation] Evaluation of Interbilayer Transport and Flip-Flop of Lipids in Vesicles by Time-Resolved Small-Angle Neutron Scattering (2007)
  • Author(s): Minoru Nakano
  • Organizer: The Second Japan-Taiwan Workshop on Neutron Scattering of Bio-materials, Soft-Matters and Nano-materials
  • Place of Presentation: Taipei, Taiwan
• [Presentation] アポリポタンパク質と混合脂質膜との相互作用によるディスク状複合体形成 (2006)
  • Author(s): 中野実
  • Organizer: コロイド
  • Place of Presentation: 札幌
  • Year and Date: 2006-09-14
• [Presentation] 非ラメラ相形成脂質が及ぼす二分子膜の微細構造変化 (2005)
  • Author(s): 中野実
  • Organizer: 膜シンポ
  • Place of Presentation: 京都
  • Year and Date: 2005-11-25
• [Presentation] 両親媒性ペプチドによる混合リン脂質リポソームの不安定化 (2005)
  • Author(s): 中野実
  • Organizer: コロイド
  • Place of Presentation: 宇都宮
  • Year and Date: 2005-09-08
• [Presentation] 蛍光法によるレシチン-モノオレイン混合脂質膜中の側方圧力の評価 (2005)
  • Author(s): 中野実
  • Organizer: 膜学会
  • Place of Presentation: 早稲田
  • Year and Date: 2005-05-19
• [Presentation] An Amphipathic Class A Peptide Induces Membrane Destabilization of Phosphatidylcholine/Sphingomyelin Mixed Bilayers (2005)
  • Author(s): Minoru Nakano
  • Organizer: 46th International Conference of Biochemistry of Lipids
  • Place of Presentation: Ajaccio, France
• [Book] 新しい分散・乳化の科学と応用技術の新展開 脂質エマルション(半田分担)(古澤邦夫編) (2006)
  • Author(s): 半田哲郎
  • Publisher: テクノシステム
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/seizai/
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/seizai/