| Project/Area Number |
17390036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Hoshi University |
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Principal Investigator |
NAKAZAWA Hiroyuki Hoshi University, Faculty of Pharm. Sci., Professor (50150173)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Koichi Hoshi Univ., Faculty of Pharm. Sci., Associate Professor (40386347)
ITO Rie Hoshi Univ., Faculty of Pharm. Sci., Research Associate (90398892)
IWASAKI Yusuke Hoshi Univ., Faculty of Pharm. Sci., Research Associate (10409360)
MAKINO Tsunehisa Tokai Univ., Faculty of Med., Honorary Professor (30085758)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,020,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2007: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2006: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2005: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Perfluorochemicals(PFCs) / Perfluorooctane sulfonate(PFOS) / human biological samples / maternal blood / umbilical blood / LC-MS / MS / house dust / exposure assessment / 有機フッ素系化合物(PFCs) / ヒト暴露源 / 超臨界流体抽出法(SFE) / パーフルオロオクタン酸(PFOA) / 安定同位体 / PFOA / カラムスイッチング / 血液 / 外部精度管理 |
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| Research Abstract |
For the development of the trace analysis of perfluorochemicals(PFCs) in biological samples and evaluation of human exposure were investigated by the 2005〜2007 fiscal year.
The 2005 fiscal year : For PFCs measurement of the human plasma, the analytical method using the column switching liquid chromatography-tandem mass spectrometry(LC-MS/MS) has been studied, and the method was applied to the analysis of PFCs in maternal blood and umbilical cord blood. The method is highly sensitive and selective for the monitoring of PFCs in human plasma. The proportional relation of concentration of PFCs between maternal blood and umbilical cord blood was obtained. The transport of PFCs from maternal blood into fetal blood was speculated.
The 2006 fiscal year: In order to elucidate the exposure source of PFCs, which have been detected in human blood with high frequency, the analytical method by use of supercritical fluid extraction (SFE) and LC-MS/MS was investigated for measurement of PFCs in house dust samples. The developed analytical method which used SFE for the pretreatment was successfully applied to analysis of house dusts with high sensitivity and repeatability. In comparison with other environmental samples, the concentration of PFCs in house dust is remarkably higher and considered as one of the major human exposure source.
The 2007 fiscal year : Based on the transport of PFCs to the fetus through the umbilical cord blood from the mother the analytical method of PFCs in mother's milk with highly sensitivity was carried out with solid phase extraction and LC-MS/MS. In results, it was indicated that the mother's milk was one of the transport pathway of PFCs to the infant from the mother.
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