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Control of cell proliferation and differentiation by heme bindiny transcription factor Bachl

Research Project

Project/Area Number 17390080
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionTohoku University

Principal Investigator

IGARASHI Kazuhiko  Tohoku University, SCA.,Graduaic School of Medicihe, Prefessor, 大学院医学系研究科, 教授 (00250738)

Co-Investigator(Kenkyū-buntansha) MUTO Akihiko  Tohoku University, Graduate School of Medicine, Reserch Associate, 大学院医学系研究科, 助手 (80343292)
IKURA Tsuyoshi  Tohoku University, Graduate School of Medicine, Lecturer, 大学院医学系研究科, 講師 (70335686)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2006: ¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 2005: ¥8,600,000 (Direct Cost: ¥8,600,000)
Keywordstranscription factor / cellular senescence / oxidative stress / Bach1 / heme / ubiquitin / proteasome / 遺伝子 / ストレス / 蛋白質 / 発現制御 / 免疫学
Research Abstract

The transcription repressor Bach1 is a sensor and an effecter of heme that regulates the expression of heme oxygenase-1 and globin genes. Heme binds to Bachl, inhibiting its DNA binding activity and inducing its nuclear export. We found that hemin further induced the degradation of endogenous Bachl in NIH3T3 cells, murine embryonic fibroblasts, and murine erythroleukemia cells. In contrast, succinylacetone, an inhibitor of heme synthesis, caused accumulation of Bachl in murine embryonic fibroblasts, indicating that physiological levels of heme regulated the Bachl turnover. Poly-ubiquitination and rapid degradation of overexpressed Bachl were induced by hemin treatment. HOIL-1, an ubiquitin-protein ligase which recognizes heme-bound, oxidized iron regulatory protein 2, was found to bind with Bachl when both were overexpressed in NIH3T3 cells. HOIL-1 stimulated the poly-ubiquitination of Bachl in a purified in vitro ubiquitination system depending on the intact heme binding motifs of Bachl. Expression of dominant negative HOIL-1 in murine erythroleukemia cells resulted in higher stability of endogenous Bachl, raising the possibility that the heme-regulated degradation involved HOIL-1 in murine erythroleukemia. cells. These results suggest that heme within a cell regulates the poly-ubiquitination and degradation of Bachl.
In addition, we found in this study that Bachl inhibits oxidative stress-induced cellular senescence. Bachl-deficient murine embryonic fibroblasts (MEFs) showed profound cellular senescence in response to oxygen in vitro as compared to wild-type control MEFs. Expression profiling of Bachl-deficient MEFs suggested that overexpression of several genes might be responsible for the oxygen-induced cellular senescence.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (19 results)

All 2007 2006 2005

All Journal Article (19 results)

  • [Journal Article] Bcr-Abl signaling through the PI-3/S6 kinase pathway inhibits nuclear translocation of the transcription factor Bach2 which represses the anti-apoptotic factor Hemeoxygenase-1.2007

    • Author(s)
      Yoshida, C. et al.
    • Journal Title

      Blood 109

      Pages: 1211-1219

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Heme oxygenase-1 gene enhancer manifests silencing activity in a chromatin environment prior to oxidative stress.2006

    • Author(s)
      Dohi, Y. et al.
    • Journal Title

      Antioxid. Redox Signal 8

      Pages: 60-67

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic ablation of the transcription repressor Bach1 leads to myocardial protection against ischemia/reperfusion in mice.2006

    • Author(s)
      Yano, Y. et al.
    • Journal Title

      Genes Cells 11

      Pages: 791-803

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Heme oxygenase-1 gene enhancer manifests silencing activity in a chromatin environment prior to oxidative stress.2006

    • Author(s)
      Dohi, Y et al.
    • Journal Title

      Antioxid. Redox Signal. 8

      Pages: 60-67

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic ablation of the transcription repressor Bach1 leads to myocardial protection against ischemia/reperfusion in mice.2006

    • Author(s)
      Yano, Y et al.
    • Journal Title

      Genes Cells. 11

      Pages: 791-803

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic ablation of the transcription repressor Bach 1 leads to myocardial protection against ischemia/reperfusion in mice.2006

    • Author(s)
      Yano, Y., et al.
    • Journal Title

      Genes Cells. 11

      Pages: 791-803

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The heme-Bach1 pathway in the regulation of oxidative stress response and erythroid differentiation.2006

    • Author(s)
      Igarashi, K., et al.
    • Journal Title

      Antioxid. Redox Signal. 8

      Pages: 107-118

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Heme oxygenase-1 gene enhancer manifests silencing activity in a chromatin environment prior to oxidative stress.2006

    • Author(s)
      Dohi, Y., et al.
    • Journal Title

      Antioxid. Redox Signal. 8

      Pages: 60-67

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Plasmacytic transcription factor Blimp-1 is repressed by Bach2 in B cells.2006

    • Author(s)
      Ochiai, K., et al.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 38226-38234

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Differential gene expression profiling between wild-type and ALAS2-null erythroblasts : Identification of novel heme-regulated genes.2006

    • Author(s)
      Fujiwara, T., et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 340

      Pages: 105-110

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Heme oxygenase-1 gene enhancer manifests silencing activity in a chromatin environment prior to oxidative stress.2006

    • Author(s)
      Dohi, Y. et al.
    • Journal Title

      Antioxid.Redox Signal. 8

      Pages: 60-67

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of genetic ablation of bach1 upon smooth muscle cell Proliferation and atherosclerosis after cuff injury.2005

    • Author(s)
      Omura.S.et al.
    • Journal Title

      Genes Cells 10

      Pages: 277-285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dynamic cytoplasmic anchoring of the transcription factor Bach1 by intracellular hyaluronic acid binding protein IHABP.2005

    • Author(s)
      Yamasaki, C. et al.
    • Journal Title

      J. Biochem 136

      Pages: 287-296

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of genetic ablation of bach1 upon smooth muscle cell proliferation and atherosclerosis after cuff injury.2005

    • Author(s)
      Omura, S. et al.
    • Journal Title

      Genes Cells 10

      Pages: 277-285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Dynamic cytoplasmic anchoring of the transcription factor Bach1 by intracellular hyaluronic acid binding protein IHABP.2005

    • Author(s)
      Yamasaki, C. et al.
    • Journal Title

      J.Biochem. 136

      Pages: 287-296

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transgenic expression of Bach1 transcription factor results in megakaryocytic impairment.2005

    • Author(s)
      Toki, T. et al.
    • Journal Title

      Blood 105

      Pages: 3100-3108

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Dynamic cytoplasmic anchoring of the transcription factor Bach1 by intracellular hyaluronic acid binding protein IHABP.2005

    • Author(s)
      Yamasaki, C. et al.
    • Journal Title

      J.Biochem 136

      Pages: 287-296

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Plycomb group gene mel-18 regulates early T progenitor expansion by maintaining the expression of Hes-1,a target of the Notch pathway.2005

    • Author(s)
      Miyazaki, M. et al.
    • Journal Title

      J.Exp.Med. 174

      Pages: 2507-2516

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The superoxide-producing NAD(P)H oxidase Nox4 in the nucleus of human vascular endothelial cells.2005

    • Author(s)
      Kuroda, J. et al.
    • Journal Title

      Genes Cells 10

      Pages: 1139-1151

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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