| Project/Area Number |
17390089
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
AKIYAMA Tetsu The University of Tokyo, Institute of Molecular and Cellular Biosciences, Professor (70150745)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2006: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2005: ¥10,700,000 (Direct Cost: ¥10,700,000)
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| Keywords | apoptosis / RNA-binding protein / KH domain / p53 / TGF-beta / estrogen / TPA / splicing / D8 / TGF-b / Bim / 3'-UTR / ノックアウトマウス / PNA |
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| Research Abstract |
We have identified and characterized a novel RNA-binding protein, termed D8 and obtained the following results:
1) D8 plays a critical role in DNA stress-induced apoptosis
2) D8 stabilizes Bim mRNA by binding to its 3'-UTR. Both KH domain 1 and 2 are required for the stabilization of Bim mRNA.
3) Bim induction is important for D8-mediated apoptosis.
4) The promoter region of D8 was directly activated by p53.
5) D8 interacts with the estrogen receptor and plays a role in mRNA splicing.
6) D8 has three family members and they are induced by TPA treatment.
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