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Molecular mechanisms of nitric oxide-dependent modification and regulation of genome functions

Research Project

Project/Area Number 17390097
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKumamoto University

Principal Investigator

AKAIKE Takaaki  Kumamoto University, Grad. Sch. of Med. Sci., Professor (20231798)

Co-Investigator(Kenkyū-buntansha) ARIMOTO Hirokazu  Tohoku University, Grad. Sch. of Life Sci., Professor (60262789)
SAWA Tomohiro  Kumamoto University, Grad. Sch. of Med. Sci, Associate Professor (30284756)
芥 照夫  熊本大学, 大学院・医学薬学研究部, 助手 (00346975)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥14,870,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2007: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2006: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 2005: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsNitric oxide / 8-nitroguanosine / 8-nitro-cGMP / signal transduction / S-guanylation / post-translational modification / genome mutation / oxidative stress / NO / 炎症 / 発がん / ニトロ化ストレス / DNA損傷 / ゲノム / 8-ニトログアニン / 変異原性 / 修復システム / デナイトラーゼ / 核酸損傷 / 脱プリン反応
Research Abstract

Excess production of free radical species including superoxide and nitric oxide (NO) has been implicated in pathophysiology of infectious and inflammatory diseases, by causing chemical modifications of biological molecules. In the present study, we investigated the roles of genome modifications and regulation by NO, with particular focus on the formation and biological effects of 8-nitroguanosine, a unique product of NO-mediated nucleic acid modification. Followings are summary of the present study.
1. 8-Nitroguanosine was found to be mutagenic to mammalian cultured cells. CHO cells treated with 8-nitroguanosine exhibited remarkably higher mutation frequency of the marker gene gpt, with G to T transversion as a dominant form of mutation, compared with control cells. Furthermore, 8-nitroguanosine treatment induced enhanced formation of abasic sites in genome DNA of CHO cells.
2. Formation of 8-nitroguanosine was significantly stimulated in the regenerative epithelium of the lung tissues of idiopathic pulmonary fibrosis. Importantly, lung squamous cell carcinoma cells were also strongly stained with 8-nitroguanosine immunohistochemistry. It was found that 8-nitroguanine was excreted in human urine, that was associated with cigarette smoking.
3. 8-Nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP) was identified, for the first time, in mammalian cells as a novel nitrated derivative of cGMP. 8-Nitro-cGMP was found to be highlyl reactive with cysteine sulfhydryls to form cGMP adduct (S-guanylation), that can be involved in cellular signaling, especially in oxidative stress response, as a unique post-translational modification.
These data suggest that guanine nitration may be a novel signaling mechanism mediated by NO. that can regulate genome stability as well as stress responses. Better understanding of the mechanisms how 8-nitro-cGMP regulates cellular signaling will give insights into the molecular pathogenesis of infectious and inflammatory diseases.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (100 results)

All 2008 2007 2006 2005 Other

All Journal Article (86 results) (of which Peer Reviewed: 27 results) Book (7 results) Remarks (1 results) Patent(Industrial Property Rights) (6 results)

  • [Journal Article] Mutagenicity of 8-nitroguanosine,a product of nitrative nucleoside modification by reactive nitrogen oxides,in mammalian cells2008

    • Author(s)
      Kaneko, K., et. al.
    • Journal Title

      Cancer Lett 262

      Pages: 239-247

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Suppression of NO production and 8-nitroguanosine formation by phenol-containing endocrine-disrupting chemicals in LPS-stimulated macrophages:Involvement of estrogen receptor-dependent or-independent pathways2008

    • Author(s)
      Yoshitake, J., et. al.
    • Journal Title

      Nitric Oxide 18

      Pages: 223-228

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nitric oxide produced in Peyer's patches exhibits antiapoptic activity contributing to an antimicrobial effect in murine salmonellosis2008

    • Author(s)
      Alam, M. S., et. al.
    • Journal Title

      Microbiol.Immunol. 52

      Pages: 197-208

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Angiopoietin-related growth factor enhances blood flow via activation of the ERKl/2-eNOS-NO pathway in a mouse hind-limb ischemia model2008

    • Author(s)
      Urano, T., et. al.
    • Journal Title

      Arterioscler.Thromb.Vasc.Biol. 28

      Pages: 827-834

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 肺の感染炎症病態におけるニトロ化ストレスとそのバイオマーカー2008

    • Author(s)
      岡本 竜哉, 他
    • Journal Title

      医学のあゆみ 224

      Pages: 851-856

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 活性酸素を消去する物質8-ニトロcGMP2008

    • Author(s)
      澤 智裕, 他
    • Journal Title

      検査と技術 (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mutagenicity of 8-nitroguanosine, a product of nitrative nucleoside modification by reactive nitrogen oxides, in mammalian cells2008

    • Author(s)
      Kaneko, K, et. al.
    • Journal Title

      Cancer Lett. 262

      Pages: 239-247

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Suppression of NO production and 8-nitroguanosine formation by phenol-containing endocrine-disrupting chemicals in LPS-stimulated macrophages : Involvement of estrogen receptor-dependent or independent pathways2008

    • Author(s)
      Yoshitake, J, et. al.
    • Journal Title

      Nitric Oxide 18

      Pages: 223-228

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nitric oxide produced in Peyer's patches exhibits antiapoptic activity contributing to an antimicrobial effect in murine salmonellosis2008

    • Author(s)
      Alam, M, S, et. al.
    • Journal Title

      Microbiol. Immunol. 52

      Pages: 197-208

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Angiopoietin-related growth factor enhances blood flow via activation of the ERK1/2-eNOS-NO pathway in a mouse hind-limb ischemia model.2008

    • Author(s)
      Urano, T, et. al.
    • Journal Title

      Arterioscler. Thromb. Vasc. Biol. 28

      Pages: 827-834

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article]2008

    • Author(s)
      Kaneko, K., et. al.
    • Journal Title

      Cancer Lett 262

      Pages: 239-247

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of NO production and 8-nitroguanosine formation by phenol-containing endocrine-disrupting chemicals in LPS-stimulated macrophages: Involvement of estrogen receptor-dependent or-independent pathways2008

    • Author(s)
      Yoshitake, J., et. al.
    • Journal Title

      Nitric Oxide 18

      Pages: 223-228

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitric oxide produced in Peyer's patches exhibits antiapoptic activity contributing to an antimicrobial effect in murine salmonellosis2008

    • Author(s)
      Alam, M.S., et. al.
    • Journal Title

      Microbiol.Immunol (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Angiopoietin-related growth factor enhances blood flow via activation of the ERK1/2-eNOS-NO pathway in a mouse hind-limb ischemia model2008

    • Author(s)
      Urano, T., et. al.
    • Journal Title

      Arterioscler.Thromb.Vasc.Biol (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 肺の感染炎症病態におけるニトロ化ストレスとそのバイオマーカー2008

    • Author(s)
      岡本 竜哉, 赤池 孝章
    • Journal Title

      医学のあゆみ 224

      Pages: 851-856

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 活性酸素を消去する物質8・ニトロcGMP2008

    • Author(s)
      澤 智裕, 赤池 孝章
    • Journal Title

      検査と技術 (印刷中)

    • Related Report
      2007 Annual Research Report
  • [Journal Article] S-Nitrosylation of human variant albumin Liprizzi(R410C)confers potent antibacterial and cytoprotective prperties2007

    • Author(s)
      Ishima, Y., et. al.
    • Journal Title

      J.Pharmacol.Exp.Ther. 320

      Pages: 969-977

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Protein S-guanylation by the biological signal 8-nitroguanosine3',5'-cyclic monophosphate2007

    • Author(s)
      Sawa, T., et. al.
    • Journal Title

      Nature Chem.Biol. 3

      Pages: 727-735

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects of endogenous ligands on the biological role of human serum albumininS-nitrosvlation2007

    • Author(s)
      Ishima, Y., et. al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 364

      Pages: 790-795

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nitrative stress in respiratory inflammation caused by influenza virus infection2007

    • Author(s)
      Zaki, M. H., et. al.
    • Journal Title

      Clin.Exp.Aller.Rev. 7

      Pages: 19-26

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] RIの逆襲:意外と使えるアイソトープ:酸化ストレス研究におけるアイソトープの活用2007

    • Author(s)
      岡本 竜哉, 他
    • Journal Title

      細胞工学 26

      Pages: 582-588

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 特発性肺線維症におけるグアニンのニトロ化損傷と発癌との関連2007

    • Author(s)
      赤池 孝章, 他
    • Journal Title

      Lung Cancer Update 46

      Pages: 1238-1238

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] iNosノックアウトマウス2007

    • Author(s)
      岡本 竜哉, 他
    • Journal Title

      分子呼吸器病 11

      Pages: 96-103

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] NOによる病原体遺伝子変異と感染制御異常2007

    • Author(s)
      岡本 竜哉, 他
    • Journal Title

      日本臨床増刊号新感染症学(上) 65,Supp1.2

      Pages: 78-84

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] S-Nitrosylation of human variant albumin Liprizzi(R410C) confers potent antibacterial and cytoprotective properties2007

    • Author(s)
      Ishima, Y., et. al.
    • Journal Title

      J. Pharmacol. Exp. Ther. 320

      Pages: 969-977

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Protein S-guanylation by the biological signal 8-nitroguanosine 3', 5'-cyclic monophosphate2007

    • Author(s)
      Sawa, T., et. al.
    • Journal Title

      Nature Chem. Biol. 3

      Pages: 727-735

    • NAID

      120002467822

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of endogenous ligands on the biological role of human serum albumin inS-nitrosylation2007

    • Author(s)
      Ishima, Y., et. al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 364

      Pages: 790-795

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nitrative stress in respiratory inflammation caused by influenza virus infection2007

    • Author(s)
      Zaki, M.H, et. al.
    • Journal Title

      Clin. Exp. Aller. Rev. 7

      Pages: 19-26

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Protein S-guanylation by the biological signal 8-nitroguanosine 3',5'-cyclic monophosphate2007

    • Author(s)
      Sawa, T., et. al.
    • Journal Title

      Nature Chem.Biol 3

      Pages: 727-735

    • NAID

      120002467822

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] S-Nitrosylation of human variant albumin Liprizzi (R410C) confers potent antibacterial and cytoprotective properties2007

    • Author(s)
      Ishima, Y., et. al.
    • Journal Title

      J.Pharmacol.Exp.Ther 320

      Pages: 969-977

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of endogenous ligands on the biological role of human serum albumin inS-nitrosylation2007

    • Author(s)
      Ishima, Y., et. al.
    • Journal Title

      Biochem.Biophys.Res.Commun 364

      Pages: 790-795

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2007

    • Author(s)
      Zaki, M.H., et. al.
    • Journal Title

      Clin.Exp.Aller.Rev 7

      Pages: 19-26

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 特発性肺線維症におけるグアニンのニトロ化損傷と発癌との関連2007

    • Author(s)
      赤池 孝章, 岡本 竜哉
    • Journal Title

      Lung Cancer Update 46

      Pages: 1238-1238

    • Related Report
      2007 Annual Research Report
  • [Journal Article] iNosノックアウトマウス2007

    • Author(s)
      岡本 竜哉, 赤池 孝章
    • Journal Title

      分子呼吸器病 11

      Pages: 96-103

    • Related Report
      2007 Annual Research Report
  • [Journal Article] NOによる病原体遺伝子変異と感染制御異常2007

    • Author(s)
      岡本 竜哉, 他
    • Journal Title

      日本臨床増刊号新感染症学 (上) 65, Suppl.2

      Pages: 78-84

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Nitrative stress in respiratory inflammation caused by influenza virus infection.2007

    • Author(s)
      Zaki, M.H.et al.
    • Journal Title

      J.Clin.Exper.Aller.Rev. (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] S-Nitrosylation of human variant albumin Liprizzi(R410C)confers potent antibacterialo and cytoprotective properties.2007

    • Author(s)
      Ishima, Y.et al.
    • Journal Title

      J.Pharmacol.Exp.Ther. 320

      Pages: 969-977

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nitrative stress through formation of 8-nitroguanosine:insights into microbial pathogenesis2006

    • Author(s)
      Akuta, T., et. al.
    • Journal Title

      Nitric Oxide 14

      Pages: 101-108

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Analysis of urinary 8-nitroguanine,a marker of nitrative nucleic acid damage,by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification:association with ciearette smoking2006

    • Author(s)
      Sawa, T., et. al.
    • Journal Title

      Free Radic.Biol.Med. 40

      Pages: 711-720

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Influence of membrane fluidity on human immunodeficiency virus typelentry2006

    • Author(s)
      Harada, S., et. al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 329

      Pages: 480-486

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Involvement of Salmonella enterica serovar Typhi RpoS in resistance to NO-mediated host defense against serovar Typhi infection2006

    • Author(s)
      Alam, M. S., et. al.
    • Journal Title

      Microb.Pathog. 40

      Pages: 116-125

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 8-nitroguanine,a product of nitrative DNA damage caused by reactive nitrogen species:formation,occurrence,and implications in inflammation and carcinogenesis2006

    • Author(s)
      Ohshima, H., et. al.
    • Journal Title

      Antioxid.Redox Signal 8

      Pages: 1033-1045

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Phosphoinositide3-kinase in nitric oxide synthesis in macrophage:critical dimerization of inducible nitric-oxide synthase2006

    • Author(s)
      Sakai, K., et. al.
    • Journal Title

      J.Biol.Chem. 281

      Pages: 17736-17742

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nitric oxide-induced downregulation ofleptin production by 3T3-L1 adipocytes2006

    • Author(s)
      Unno, Y., et. al.
    • Journal Title

      Nitric Oxide 15

      Pages: 125-132

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis2006

    • Author(s)
      Terasaki, Y., et. al.
    • Journal Title

      Am.J.Respir.Crit.Care Med. 174

      Pages: 665-673

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 一酸化窒素(NO)による新しい感染防御機構2006

    • Author(s)
      岡本 竜哉, 他
    • Journal Title

      医学のあゆみ 酸化ストレスver2

      Pages: 219-223

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] バイオスピンのケミカルバイオロジーとシグナル伝達2006

    • Author(s)
      赤池 孝章
    • Journal Title

      細胞工学 25

      Pages: 126-127

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] ニトロ化修飾反応によるNOの新しいシグナル機構2006

    • Author(s)
      澤 智裕, 他
    • Journal Title

      細胞工学 25

      Pages: 132-136

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] NOガスが病原菌をやっつける:NOによる生体分子のニトロ化およびニトロソ化を介するユニークな抗菌作用2006

    • Author(s)
      澤 智裕, 他
    • Journal Title

      現代化学 424

      Pages: 51-56

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 敗血症とショックの病態2006

    • Author(s)
      田村 文雄, 他
    • Journal Title

      化学療法の領域 22

      Pages: 19-24

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nitrative stress through formation of 8-nitroguanosine : insights into microbial pathogenesis2006

    • Author(s)
      AKuta T., et. al.
    • Journal Title

      Nitric Oxide 14

      Pages: 101-108

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification : association with cigarette smoking2006

    • Author(s)
      Sawa, T., et. al.
    • Journal Title

      Free Radic. Biol. Med. 40

      Pages: 711-720

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Influence of membrane fluidity on human immunodeficiency virus type 1 entry2006

    • Author(s)
      Harada, S., et. al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 329

      Pages: 480-486

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Involvement of Salmonella enterica serovar Typhi RpoS in resistance to NO-mediated host defense against serovar Typhi infection2006

    • Author(s)
      Alam, M.S, et. al.
    • Journal Title

      Microb. Pathog. 40

      Pages: 116-125

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 8-nitroguanine, a product of nitrative DNA damage caused by reactive nitrogen species : formation, occurrence, and implications in inflammation and carcinogenesis2006

    • Author(s)
      Ohshima, H, et. al.
    • Journal Title

      Antioxid. Redox Signal. 8

      Pages: 1033-1045

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Phosphoinositide 3-kinase in nitric oxide synthesis in macrophage : critical dimerization of inducible nitric-oxide synthase2006

    • Author(s)
      Sakai, K, et. al.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 17736-17742

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nitric oxide-induced downregulation of leptin production by 3T3-L1 adipocytes2006

    • Author(s)
      Unno, Y., et. al.
    • Journal Title

      Nitric Oxide 15

      Pages: 125-132

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis2006

    • Author(s)
      Terasaki. Y., et. al.
    • Journal Title

      Am. J. Respir. Crit. Care Med. 174

      Pages: 665-673

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis.2006

    • Author(s)
      Terasaki, Y.et al.
    • Journal Title

      Am.J.Respir.Crit.Care Med. 174

      Pages: 665-673

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification : association with cigarette smoking.2006

    • Author(s)
      Sawa, T.et al.
    • Journal Title

      Free Radic.Biol.Med. 40

      Pages: 711-720

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 8-Nitroguanine, a product of nitrative DNA damage caused by reactive nitrogen species : formation, occurrence, and implications in inflammation and carcinogenesis.2006

    • Author(s)
      Ohshima, H.et al.
    • Journal Title

      Antioxid.Redox Signal. 8

      Pages: 1033-1045

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nitric oxide-induced downregulation of leptin production by 3T3-L1 adipocytes.2006

    • Author(s)
      Unno, Y.et al.
    • Journal Title

      Nitric Oxide 15

      Pages: 125-132

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Involvement of Salmonella enterica serovar Typhi RpoS in resistance to NO-mediated host defense against serovar Typhi infection.2006

    • Author(s)
      Alam, M.S.et al.
    • Journal Title

      Microb.Pathog. 40

      Pages: 116-125

    • Related Report
      2006 Annual Research Report 2005 Annual Research Report
  • [Journal Article] Nitrative stress through formation of 8-nitroguanosine : insights into microbial pathogenesis.2006

    • Author(s)
      Akuta, T.et al.
    • Journal Title

      Nitric Oxide 14

      Pages: 101-108

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Phosphoinositide 3-kinase in nitric oxides synthesis in macrophage : critical dimerization of inducible nitric oxide synthase.2006

    • Author(s)
      Sakai, K.et al.
    • Journal Title

      J.Biol.Chem. 281

      Pages: 17736-17742

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nitrative stress through formation of 8-nitroguanosine : Insights into microbial pathogenesis.2006

    • Author(s)
      Akuta, T. et al.
    • Journal Title

      Nitric Oxide 14

      Pages: 101-108

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification : Association with cigarette smoking.2006

    • Author(s)
      Sawa, T. et al.
    • Journal Title

      Free Radic.Biol.Med. 40

      Pages: 711-720

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Influence of membrane fluidity on human immunodeficiency virus type 1 entry.2006

    • Author(s)
      Harada, S. et al.
    • Journal Title

      Biochem Biophys Res Commun. 329

      Pages: 480-486

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 8-Nitroguanine, a product of nitrative DNA damage caused by reactive nitrogen species : Formation, occurrence and implications in inflammation and carcinogenesis.2006

    • Author(s)
      Ohshima, H. et al.
    • Journal Title

      Antioxidants Redox Signal. (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nitric oxide-induced downregulation of leptin production by 3T3-L1 adipocytes.2006

    • Author(s)
      Unno, Y. et al.
    • Journal Title

      Nitric Oxide (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 敗血症とショックの病態2006

    • Author(s)
      田村 文雄 他
    • Journal Title

      化学療法の領域 22(3)

      Pages: 19-24

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Interleukin-1beta induces death in chondrocyte-like ATDC5 cells through mitochondrial dysfunction and energy depletion in a reactive nitrogen and oxygen species-dependent manner2005

    • Author(s)
      Yasuhara, R., et. al.
    • Journal Title

      Biochem.J. 389

      Pages: 315-323

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nitric oxide-induced nitrative stress involved in microbial pathogenesis2005

    • Author(s)
      Zaki, J. H., et. al.
    • Journal Title

      J.Pharmacol.Sci. 8

      Pages: 117-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of nitric oxide in amyloid fibril rormation on transthyretin-related amyloidosis2005

    • Author(s)
      Saito, S., et. al.
    • Journal Title

      Biochemistry 44

      Pages: 1122-1129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nitric oxide(NO)およびNO関連物質2005

    • Author(s)
      芥 照夫, 他
    • Journal Title

      日本臨床 63

      Pages: 791-793

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] プリン異化代謝と活性酸素・NO:尿酸の抗酸化作用とニトログアニンのシグナル機能2005

    • Author(s)
      芥 照夫, 他
    • Journal Title

      Gout and Nucleic Acid Metabolism 29

      Pages: 157-157

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 一酸化窒素(NO)の新しいシグナル伝達機構-グアノシンのニトロ化を介するNOシグナル2005

    • Author(s)
      芥 照夫, 他
    • Journal Title

      化学と生物 43

      Pages: 420-422

    • NAID

      10019044475

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 注目される酸化ストレスマーカー:NOマーカー2005

    • Author(s)
      芥 照夫, 他
    • Journal Title

      臨床検査 49

      Pages: 139-143

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] レドックスーストレス防御の医学2005

    • Author(s)
      芥 照夫, 他
    • Journal Title

      医学のあゆみ 別冊NO biology

      Pages: 56-60

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 肺の炎症病態におけるNO・活性酸化窒素種の生成とマトリックス・メタロプロテアーゼの活性発現調節2005

    • Author(s)
      岡本 竜哉, 他
    • Journal Title

      医学のあゆみ 214

      Pages: 919-925

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Interleukin-lbeta induces death in chondrocyte-like ATDC5 cells through mitochondrial dysfunction and energy depletion in a reactive nitrogen and oxygen species-dependent manner2005

    • Author(s)
      Yasuhara, R, et. al.
    • Journal Title

      Biochem. J. 389

      Pages: 315-323

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nitric oxide-induced nitrative stress involved in microbial pathogenesis2005

    • Author(s)
      Zaki, J.H, et. al.
    • Journal Title

      J. Pharmacol. Sci. 8

      Pages: 117-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of nitric oxide in amyloid fibril formation on transthyretin-related amyloidosis2005

    • Author(s)
      Saito, S, et. al.
    • Journal Title

      Biochemistry 44

      Pages: 11122-11129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Interleukin-1β induces death in chondrocyte-like ATDC5 cells through mitochondrial dysfunction and energy depletion in a reactive nitrogen and oxygen species-dependent manner.2005

    • Author(s)
      Yasuhara, R. et al.
    • Journal Title

      Biochem.J. 389

      Pages: 315-323

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nitric oxide-induced nitrative stress involved in microbial pathogenesis.2005

    • Author(s)
      Zaki, M.H.et al.
    • Journal Title

      J.Pharmacol.Sci. 8

      Pages: 117-129

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effect of nitric oxide in amyloid fibril formation on transthyretin-related amyloidosis.2005

    • Author(s)
      Saito, S. et al.
    • Journal Title

      Biochemistry 44

      Pages: 11122-11129

    • Related Report
      2005 Annual Research Report
  • [Book] 酸化ストレスの医学2008

    • Author(s)
      澤 智裕, 他
    • Publisher
      診断と治療社(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 酸化ストレスの医学2008

    • Author(s)
      澤 智裕, 赤池 孝章
    • Publisher
      診断と治療社(印刷中)
    • Related Report
      2007 Annual Research Report
  • [Book] 細胞工学別冊実験プロトコールシリーズRIの逆襲2007

    • Author(s)
      岡本 竜哉, 他
    • Total Pages
      185
    • Publisher
      秀潤社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 細胞工学別冊実験プロトコールシリーズ, RIの逆襲2007

    • Author(s)
      岡本 竜哉, 他
    • Publisher
      秀潤社
    • Related Report
      2007 Annual Research Report
  • [Book] 敗血症の解明と治療戦略2006

    • Author(s)
      田村 文雄, 他
    • Total Pages
      382
    • Publisher
      医薬ジャーナル社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 敗血症の解明と治療戦略2006

    • Author(s)
      田村 文雄, 赤池 孝章
    • Total Pages
      382
    • Publisher
      株式会社 医薬ジャーナル社
    • Related Report
      2005 Annual Research Report
  • [Book] 酸化ストレスマーカー2005

    • Author(s)
      赤池 孝章
    • Total Pages
      300
    • Publisher
      学会出版センター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://kumadai-bisei.com

    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] SH基修飾剤2007

    • Inventor(s)
      赤池 孝章, 有本 博一, 澤 智裕
    • Industrial Property Rights Holder
      熊本大学
    • Industrial Property Number
      2007-252877
    • Filing Date
      2007-09-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 8-チオアルコキシグアノシン3', 5'-サイクリック1リン酸を特異的に認識する抗体2007

    • Inventor(s)
      赤池 孝章, 澤 智裕
    • Industrial Property Rights Holder
      熊本大学
    • Industrial Property Number
      2007-015728
    • Filing Date
      2007-01-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] SH修飾剤2007

    • Inventor(s)
      赤池孝章, 有本博一, 澤 智裕
    • Industrial Property Rights Holder
      熊本大学
    • Industrial Property Number
      2007-015727
    • Filing Date
      2007-01-26
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 8-チオアルコキシグアノシン-3'、5'-サイクリック1リン酸を特異的に認識する抗体2007

    • Inventor(s)
      赤池孝章, 澤 智裕
    • Industrial Property Rights Holder
      熊本大学
    • Industrial Property Number
      2007-015728
    • Filing Date
      2007-01-26
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 抗8-ニトロサイクリックグアノシン3', 5'-1リン酸抗体2005

    • Inventor(s)
      赤池 孝章, 芥 照夫, 有本 博一, 佐々本 一美
    • Industrial Property Rights Holder
      熊本大学名古屋大学同仁化学研究所
    • Industrial Property Number
      2005-125838
    • Filing Date
      2005-04-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 抗8-ニトロサイクリックグアノシン3'、5'-一リン酸抗体2005

    • Inventor(s)
      赤池孝章, 芥 照夫, 有本博一, 佐々本一美
    • Industrial Property Rights Holder
      熊本大学, 名古屋大学, 同仁化学研究所
    • Industrial Property Number
      2005-125838
    • Filing Date
      2005-04-25
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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