| Project/Area Number |
17390100
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human genetics
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| Research Institution | Kumamoto University |
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Principal Investigator |
ENDO Fumio Kumamoto University, Faculty of Medical and Pharmaceutical Sciences, Professor, 大学院医学薬学研究部, 教授 (00176801)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Tetsuro Kumamoto University, Faculty of Medical and Pharmaceutical Sciences, Professor, 大学院医学薬学研究部, 教授 (60112405)
NAKAMURA Kimitoshi Kumamoto University, University, Hospital, Research Associates, 医学部附属病院, 助手 (30336234)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2006: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2005: ¥8,600,000 (Direct Cost: ¥8,600,000)
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| Keywords | hepatocyte / stem cell / liver injury / regenerative medicine / genetics / amino acid / metabolic disease / 内胚葉 / DNAチップ / 遺伝性疾患 |
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| Research Abstract |
We attempted to reorganize hepatobiliary system by means of transplantation of progenitor cells derived from salivary gland. Our achievement should be useful for therapy not only hepatic but also for biliary and pancreatic diseases. We transplanted gene modified hepatic cells into model mice for hereditary hepatic disease. Using the cell transplantation, we identified new endodermal stem cells. We established technique to replace damaged hepatocyte of the model mice with gene modified hepatocyte. Furthermore, we found method for separating hepatic progenitor cells from adult. Next, we established method for separating stern cells of liver and pancreas. We can produce hepatic and pancreatic cells in vivo and in vitro. We established method to isolate hepatic and pancreatic cells derived from pluripotent stem cells. In this method, GFP labeled cells were recognized as pancreatic progenitor cells. We investigated gene expressions of progenitor cells isolated by this method. We have got new founding after investigation of gene expression of translation factors.
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