| Project/Area Number |
17390105
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | YOKOHAMA CITY UNIVERSITY |
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Principal Investigator |
AOKI Ichiro YOKOHAMA CITY UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, DEPARTMENT OF MOLECULAR PATHOLOGY AND ONCOLOGY, PROFESSOR, 医学研究科, 教授 (00184028)
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| Co-Investigator(Kenkyū-buntansha) |
RYO Akihide GRADUATE SCOOL OF MEDICINE, DEPARTMENT OF MOLECULAR PATHOLOGY AND ONCOLOGY, ASSOCIATE PROFESSOR, 医学研究科, 準教授 (20363814)
NAGASHIMA Yoji GRADUATE SCOOL OF MEDICINE, DEPARTMENT OF MOLECULAR PATHOLOGY AND ONCOLOGY, ASSOCIATE PROFESSOR, 医学研究科, 準教授 (10217995)
SASAKI Takeshi YOKOHAMA CITY UNIVERSITY, MEDICAL CENTER, ASSOCIATE PROFESSOR, 附属市民総合医療センター, 準教授 (30225875)
INAYAMA Yoshiaki YOKOHAMA CITY UNIVERSITY, HOSPITAL, ASSOCIATE PROFESSOR, 附属病院, 準教授 (10184730)
HIRANO Hisashi KIHARA INSTITUTE FOR BIOLOGYCAL RESEARCH, ASSOCIATE PROFESSOR, 国際総合科学研究科, 教授 (00275075)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 2006: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2005: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | protein / phosphated / cancer / isomerase / proteomics / prostate / 前立腺 / Pin1 / 前立腺癌 / 分子標的 |
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| Research Abstract |
Pin1 is novel type of enzyme which binds specifically with phosphated protein to change its three dimensional structure and modulates the function. Recent investigations by us and others strongly suggest the close involvement of Pin1 to the biological behavior of certain cancers. Prostate cancer is one of the representative examples. Thus we conducted the investigations to fish the proteins which are phosphorylated specifically in prostate cancer and bind to Pinl by using Pinl as a molecular probe. The investigation successfully found several proteins which are phosphorylated and bind to Pinl specifically in prostate cancer. Some of them are novel ones as a prostate cancer specific protein. Among them the biological activities of PIN1BP1 were analyzed to reveal the potential prognostic factor and molecular target for therapeutics.
In addition several other novel findings were revealed during the investigations.
1 RelA is phosphorylated and binds to Pin1 specifically in prostate cancer. The anti-phosphorylated RelA antibody discriminates the cases of prostate cancer which showed bone metastasis from the cases without bone metastasis. Thus this antibody could be a good prognosis indicator.
2 Gliomas showed increased expression of Pin1. The expression correlates the biological behavior of gliomas.
3 Pin1 binds the phosphorylated Daxx protein to prevent the cells from apoptosis.
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