| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥5,300,000 (Direct Cost: ¥5,300,000)
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| Research Abstract |
Adult T-cell leukemia/lymphoma (ATLL) have very complicated karyotypic abnormalities, and hence it is difficult to detect cytogenetic rearrangements merely by conventional chromosomal banding method. 45 patients and 7 established cell lines were subjected to the current study. Combined multicolor spectral karyotyping (SKY) and DAPI banding analysis was performed as described previously (Genes Chromosomes Cancer, 26:336,1999). Structural chromosome abnormalities of translocations, inversions, insertions, isochromosomes, and deletions frequently involved 7 and 14, which contain four TCR genes. /Breakages most frequently occurring at 10p11-13 (46.6%), and 14q11.2 (35.6%), 14q32 (26.7%),7q22 (22.2%), 9q22 (22.2%), 13q14 (20.0%), 22p11 (17.7%), 8p11 (15.5%), 18p11 (15.5%), 21q22 (15.5%) and 19q13 (13.3%). Recurrent chromosomal rearrangements associated with 10p11-13 were 21q22 (3cases), 13q14 (2 cases) and 14q32.1 (2 cases). Recurrent partner chromosomal break points with 14811.2 were 14q32 (3 cases), 8p 11.2 or 8p21 (3case) and 11q13.3 (2 cases). Recurrent partners with 14q32 were 14q11 (3 cases) and 10p 11-13 (2 cases). These chromosomal breakpoints provide a landmark to identify genes implicated in pathogenesis of ATLL.
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