| Project/Area Number |
17390195
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Public health/Health science
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| Research Institution | Kanagawa Cancer Center Research Institute |
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Principal Investigator |
OKAMOTO Naoyuki Kanagawa Cancer Center Research Institute, Cancer Prevention and Cancer Control Division, Research Inst., Kanagawa Cancer Center, Research Head (90116319)
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| Co-Investigator(Kenkyū-buntansha) |
TOCHIKUBO Osamu Yokohama City University School of Medicine, Dept. of Public Health, Professor (30046099)
MIYAGI Yohei Research Inst, Kamakura Cancer Center, Molecular Pathology Division, Research Head (00254194)
矢野間 俊介 神奈川県立がんセンター(臨床研究所), がん予防・情報研究部門, 研修生 (30182398)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥13,650,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2007: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | Cancer / Preventive Medicine / Screening / Tumor Marker |
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| Research Abstract |
The amino-acid balance is altered in patients with various diseases, due to metabolic changes. The metabolism in cancer cells is also known to be notably altered. Amino-acid profiles could therefore be useful biomarkers of various cancers. In this study, significant changes of amino-acid profiles were detected in the plasma of colorectal and breast cancer patients through multivariate analysis.
Venous blood samples were taken from colorectal cancer patients, breast cancer patients, and healthy controls. Plasma free amino acids were measured by liquid chromatography(LC)/mass spectrometry(MS). Based on the amino-acid profiles, multiple logistic-regression models were used to discriminate between colorectal or breast cancer patients and control subjects. Diagnostic performance was evaluated as the area under the curve(AOC) of the receiver operating characteristic(ROC) curve, and was validated with randomly divided test data.
The plasma amino-acid profiles differed between colorectal and breast cancer patients and healthy controls. No weight loss was observed in any of the cancer patients. The AUC of the discriminant ROC curve for each discriminant score was calculated as 0.863 for colorectal cancer and 0.856 for breast cancer. Reproducible performance was observed using split test data. Moreover, the performance of these discriminants was independent of the cancer stage.
The occurrence of cancer alters plasma amino-acid profiles without cachexia or weight loss, and the pattern of change differs between cancers. Plasma amino-acid profiling might therefore be useful for the low-cost early detection of several kinds of cancer.
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