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Molecular biological analysis of age-dependently expressed genes : application to age estimation in legal medicine

Research Project

Project/Area Number 17390199
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionUniversity of Fukui

Principal Investigator

IIDA Reiko  University of Fukui, 医学部, 助教 (40139788)

Co-Investigator(Kenkyū-buntansha) MATSUKI Matsuki  福井大学, 医学部, 教授 (10126617)
Project Period (FY) 2005 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥10,770,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2006: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2005: ¥3,100,000 (Direct Cost: ¥3,100,000)
Keywords年齢推定 / 老化 / 遺伝子発現 / 法医学
Research Abstract

年齢依存性発現生体分子を"マーカー"とした法医学的年齢推定法を開発するための基礎的研究を行い, 以下の成果を得た。(1) マウス肝において年齢依存性発現を示す遺伝子を検索し, 6個の既知遺伝子を同定した。(2) 若年者の尿に特異的に見い出される糖タンパク質Ugl-Yについて解析を行ない, Ugl-Yがfibronectinの一部の配列に完全に一致すること, また, 血清に由来することを明らかにした。(3) マウス腎で見い出された年齢依存性発現生体分子M-LPはヒトにも存在し, 活性酸素の代謝機構に関与していることが示された。

Report

(5 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (34 results)

All 2009 2008 2007 2006 2005 Other

All Journal Article (16 results) (of which Peer Reviewed: 14 results) Presentation (14 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Simultaneous genotyping of eleven non-synonymous SNPs in the four glutathione peroxidase genes using the multiplex single base extension method2009

    • Author(s)
      R. Iida, et al.
    • Journal Title

      Clin. Chim. Acta (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiplex single base extension method for simultaneous genotyping of non-synonymous SNPs in the three human SOD genes2008

    • Author(s)
      R. Iida, E. Tsubota, H. Takeshita, T. Yasuda
    • Journal Title

      Electrophoresis 29

      Pages: 4788-4794

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of age-dependently expressed genes in mouse liver by differential display-PCR analysis2008

    • Author(s)
      E. Tsubota, T. Yasuda, R. Iida
    • Journal Title

      Comparative Biochem. Physiol D Proteomics 3

      Pages: 91-95

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiplex single base extension method for simultaneous genotyping of non-synonymous SNPs in the three human SOD genes2008

    • Author(s)
      R. Iida, et al.
    • Journal Title

      Electrophoresis 29

      Pages: 4788-4794

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of age-dependently expressed genes in mouse liver by differential display-PCR analysis.2008

    • Author(s)
      E. Tsubota, et. al.
    • Journal Title

      Comparative Biochem. Physiol.D Genomics Proteomics 3

      Pages: 91-95

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] マウス腎の年齢依存性発現分子M-LPのヒトホモログの同定2008

    • Author(s)
      飯田礼子, 他
    • Journal Title

      DNA多型 16

      Pages: 213-216

    • NAID

      10025753724

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of novel fibronectin fragments detected specifically in juvenile urine2007

    • Author(s)
      R. Iida, T. Yasuda, K. Kishi
    • Journal Title

      FEBS J 274

      Pages: 3939-3947

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hypoxia induces up- regulation of the deoxyribonuclease I gene in the human pancreatic cancer cell line QGP-1. Biochim2007

    • Author(s)
      Y. Kominato, R. Iida, T. Nakajima,Y. Tajima, R. Takagi, C. Makita, K. Kishi, M. Ueki, Y. Kawai, T. Yasuda
    • Journal Title

      Biophys. Acta 1770

      Pages: 1567-1575

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Susceptibility of mammalian deoxyribonucleases I(DNases I)to proteolysis by proteases and its relationships to tissue distribution : biochemical and molecular analysis of equine DNase I2007

    • Author(s)
      M. Ueki, H. Takeshita, J. Fujihara, G. Ueta, T. Nakajima, Y. Kominato, K. Kishi, R. Iida, T. Yasuda
    • Journal Title

      Comparative Biochem. Physiol. B 148

      Pages: 93-102

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of human deoxyribonuclease I gene(DNASE1)promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation2006

    • Author(s)
      K. Kominato, M. Ueki, R. Iida, Y. Kawai, T. Nakajima, C. Makita, M. Itoi, Y. Tajima, K. Kishi, T. Yasuda
    • Journal Title

      FEBS J 273

      Pages: 3094-105

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human Mpv17-like protein is localized in peroxisomes and regulate expression of antioxidant enzymes2006

    • Author(s)
      R. Iida, T. Yasuda, E. Tsubota, H. Takatsuka, T. Matsuki, K. Kishi
    • Journal Title

      Biochem. Biophys. Res. Commun 344

      Pages: 948-54

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human Mpvl7-like protein is localized in peroxisoraes and regulate expression of antioxidant enzymes.2006

    • Author(s)
      R.Iida, et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 344・3

      Pages: 948-54

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Human Mpv17-like protein is localized in peroxisomes and regulate expression of antioxidant enzymes.2006

    • Author(s)
      R.Iida, et al.
    • Journal Title

      Biochemical and Biophysical Research Communications (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Quantitative change in mitochondrial DNA content in various mouse tissues during aging2005

    • Author(s)
      M. Masuyama, R. Iida, H. Takatsuka, T. Yasuda, T. Matsuki
    • Journal Title

      Biochim. Biophys. Acta 1723

      Pages: 302-308

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel alternative spliced Mpv17-like protein isoform localizes in cytosol and is expressed in a kidney-specific and adult-specific manner2005

    • Author(s)
      R. Iida, T. Yasuda, E. Tsubota, H. Takatsuka, M. Masuyama, T. Matsuki, K. Kishi
    • Journal Title

      Exp. Cell Res 302

      Pages: 22-30

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous genotyping of eleven non-synonymous SNPs in the four glutathione peroxidase genes using the multiplex single base extension method

    • Author(s)
      R. Iida, E. Tsubota, I. Yuasa, H. Takeshita, T. Yasuda
    • Journal Title

      Clin. Chim. Acta (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Presentation] Identification of novel Fibronec in fragments detected specifically in Juvenile urine2008

    • Author(s)
      飯田礼子, 安田年博
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Final Research Report
  • [Presentation] Identification of novel Fibronectin fragments detected specifically in juvenile urine2008

    • Author(s)
      飯田礼子, 他
    • Organizer
      日本分子生物学会年会・日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] 核酸分解酵素DNASEI遺伝子の転写調節機構の解明2008

    • Author(s)
      小湊慶彦, 中島たみ子, 田島裕, 高木利恵, 竹下治男, 藤原純子, 飯田礼子, 安田年博
    • Organizer
      第92次日本法医学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2008-04-24
    • Related Report
      2008 Final Research Report
  • [Presentation] マウスの年齢依存性発現分子M-LPのヒトホモログの遺伝子構造2008

    • Author(s)
      飯田礼子, 松木孝澄, 安田年博
    • Organizer
      第92次日本法医学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2008-04-24
    • Related Report
      2008 Final Research Report
  • [Presentation] マウスの年齢依存性発現分子M-LPのヒトホモログの遺伝子構造2008

    • Author(s)
      飯田礼子, 他
    • Organizer
      日本法医学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2008-04-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] 核酸分解酵素DNASEI遺伝子の転写調節機構の解明2008

    • Author(s)
      小湊慶彦, 他
    • Organizer
      日本法医学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2008-04-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] Identification and characterizaion of a human homolog of the M-LP(M-LPH) gene2007

    • Author(s)
      飯田礼子, 安田年博
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Related Report
      2008 Final Research Report
  • [Presentation] Identification and characterization of a human homolog of the M-LP(M-LPH)gene2007

    • Author(s)
      飯田 礼子
    • Organizer
      日本分子生物学会年会・日本生化学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] マウス腎の年齢依存性発現分子M-LPのヒトホモログの同定2007

    • Author(s)
      飯田礼子, 坪田悦子, 松木孝澄, 安田年博
    • Organizer
      日本DNA多型学会第16回学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2007-11-15
    • Related Report
      2008 Final Research Report
  • [Presentation] マウス腎新規タンパク質M-LPの細胞局在型イソフォームの同定2007

    • Author(s)
      飯田礼子, 坪田悦子, 松木孝澄, 安田年博
    • Organizer
      第91次日本法医学会総会
    • Place of Presentation
      秋田
    • Year and Date
      2007-05-18
    • Related Report
      2008 Final Research Report
  • [Presentation] Age-dependently expressed genes in mouse tis ues revealed by the fluorescence differential display-PCR technique2006

    • Author(s)
      R. Iida, T. Yasuda, T. Matsuki, E. Tsubota
    • Organizer
      European Conference of Aging 2006
    • Place of Presentation
      Innsbruck, Austria
    • Year and Date
      2006-11-29
    • Related Report
      2008 Final Research Report
  • [Presentation] A cytosolic isofom of Mpv17-like protein is expressed in an adult-specific manner2006

    • Author(s)
      R. Iida, T. Matsuki, T. Yasuda
    • Organizer
      20th IUBMB International Congress of Biochemisry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-06-21
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒト尿中に検出される若年者特異的タンパク質(Ugl-Y)の化学構造2006

    • Author(s)
      飯田礼子, 安田年博, 岸紘一郎
    • Organizer
      第90次日本法医学会総会
    • Place of Presentation
      福岡
    • Year and Date
      2006-04-28
    • Related Report
      2008 Final Research Report
  • [Presentation] マウスの年齢依存性発現分子, M-LP, の細胞質局在型イソフォーム2005

    • Author(s)
      飯田礼子, 高塚和, 坪田悦子, 松木孝澄, 安田年博, 岸紘一郎
    • Organizer
      日本DNA多型学会第14回学術集会
    • Place of Presentation
      前橋
    • Year and Date
      2005-11-24
    • Related Report
      2008 Final Research Report
  • [Book] マウス腎の年齢依存性発現分子M-LPのヒトホモログの同定DNA多型第16巻2008

    • Author(s)
      飯田礼子, 坪田悦子, 松木孝澄, 安田年博
    • Publisher
      東洋書店
    • Related Report
      2008 Final Research Report
  • [Book] マウスの年齢依存性発現分子, MLP, の細胞質局在型イソフォーム. DNA多型第14巻2006

    • Author(s)
      飯田礼子, 坪田悦子, 高塚尚和, 松木孝澄, 安田年博, 岸紘一郎
    • Publisher
      東洋書店
    • Related Report
      2008 Final Research Report
  • [Book] ヒトDNaseIの分子多様性と心疾患. DNA多型第14巻2006

    • Author(s)
      安田年博, 植木美鈴, 飯田礼子, 河合康幸, 小湊慶彦, 中島たみ子, 岸紘一郎
    • Publisher
      東洋書店
    • Related Report
      2008 Final Research Report
  • [Remarks] 新規塩基配列のGenBankへの登録M-LPs cDNA : AY513273, M-LPH1 : DQ004255, M-LPH2 : DQ004256, mouse liver novel EST : ES452733, ES452734

    • Related Report
      2008 Final Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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