| Project/Area Number |
17390202
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | Nagasaki University |
|---|
Principal Investigator |
NAKASONO Ichiro Nagasaki University, Graduate School of Biochemical Sciences, Professor, 大学院医歯薬学総合研究科, 教授 (30108287)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TSUDA Ryouichi Nagasaki University, Graduate School of Biochemical Sciences, Lecture, 大学院医歯薬学総合研究科, 講師 (20098875)
IKEMATSU Kazuya Nagasaki University, Graduate School of Biochemical Sciences, Associate professor, 大学院医歯薬学総合研究科, 准教授 (80332857)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2006: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2005: ¥8,700,000 (Direct Cost: ¥8,700,000)
|
|---|
| Keywords | SAGE / Asphyxia / Hanging / Mouse / Brain / IEGs / IEG s / C-fos |
|---|
| Research Abstract |
We investigated a transcriptome profile of mouse brain after hanging by serial analysis of gene expression (SAGE) method. RNA was extracted from the forebrain, hippocampus and cerebellum after hanging previously reported. Control groups were killed by decapitation without hanging. First, we investigated the level of immediate early genes (IEGs) mRNA such as c-fos, fos B and c-jun that induced another genes. As the results, the all IEGs drastically increased with hanging on all regions. These results indicated that transcriptome profiles might change with hanging. We have been able to obtain the transcriptome profiles after analyzing 12866 tags in hanging and 13334 tags in control. 7311 different genes in hanging and 7612 in control were observed. Compared with the two groups, 64 genes, including Ras homolog gene family member B and leucine-rich repeat neuronal 6A, were increased in hanging. On the other hand, 49 genes such as Kinesin family member C, and Phoshoprotein enriched in astrocytes 15 were decreased. These results contribute to clarify the pathophysiology of asphyxia in the lung and aid in the diagnosis of suffocation.
|
