| Project/Area Number |
17390214
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Yamagata University |
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Principal Investigator |
KAWATA Sumio Yamagata University, Medical School, professor, 医学部, 教授 (90183285)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Takafumi Yamagata University, Kedical School, Associate Professor, 医学部, 助教授 (80250918)
SAITO Koji Yamagata University, Medical School, Lecturer, 医学部, 講師 (90250919)
SUGAHARA Kazuhiko Yamagata University, Medical School, Assistant Professor, 医学部, 助手 (70333953)
ITO Junitsu Yamagata University, Medical School, Assistant Professor, 医学部, 助手 (90344788)
TOGASHI Hitoshi Yamagata University, Medical School, Associate Professor, 医学部, 助教授 (60192209)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2006: ¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 2005: ¥7,800,000 (Direct Cost: ¥7,800,000)
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| Keywords | hepatic failure / TPO / SCF / bone marrow cell transplantation / bone marrow cells / FGF2 / hepatic stem cells / liver regeneration therapy / 肝幹細胞 / 肝不全 / 肝再生 / 造血幹細胞増殖因子 / 劇症肝炎 / musashi-1 |
|---|
| Research Abstract |
We are trying to develop a useful therapy for severe hepatic failure with hepatic stem cells or autologous bone marrow cells.
In 2005, we found that serum levels of SCF (stem cell factor) and TPO (thrombopoetin) significantly increased in patients with acute hepatitis in comparison with those in healthy controls. On the other hand, serum levels of SCF and TPO significantly reduced in patients with fulminant hepatitis. Furthermore, the serum levels in patients who survived were significantly lower than those in patients who were dead with fulminate hepatitis.
In 2006, we found an interaction cell growth and differentiation between bone marrow cells and hepatic stem cell line in a co-culture system. Using DNA microarray, bone marrow cells produced FGF2 in co-culture system with hepatic stem cell line. The addition of GFF2 in the culture medium stimulated differentiation of hepatic stem cell line into a liver cell lineage.
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