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Development of efficient hematopoietic stem cell proliferation systems using our own established ES cells of small monkey, common marmoset

Research Project

Project/Area Number 17390279
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

TANI Kenzaburo  Kyushu University, Medical Institute of Bioregulation, Professor, 生体防御医学研究所, 教授 (00183864)

Co-Investigator(Kenkyū-buntansha) SASAKI Erika  Central Institute for Experimental Animals, Curator, 霊長類研究室, 研究員 (70390739)
SUEHIRO Yoko  Kyushu University, Hospital, Research Associate, 病院・助手 (50380522)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2006: ¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 2005: ¥9,500,000 (Direct Cost: ¥9,500,000)
KeywordsCommon marmoset / ES cell / tal1 / scl / glycophorin antibody / human fetal liver / pseudotype lentiviral vector / cDNA expression library / leukemia cell line / tall / SSEA4抗体 / tet-on / アデノウイルスベクター / 造血前駆細胞 / NOGマウス
Research Abstract

Toward the goal of establishing efficient hematopoietic stem cells and hematopoietic differentiation system from our own established common marmoset(CM) embryonic stem(ES) cells, we first established tall/scl gene transduced CMES (tal1/scl) cell lines. Tall/scl gene transduction was found to differentiate CMES cells efficiently to hematopoietic cells in vitro in our previous studies. We first of all found in vitro differentiating capacity of the tal1/scl cells to lymphocytes and megakaryocytes, although the efficiency was low. On the other hand, our in vivo studies using immune deficient NOG mice demonstrated very poor hematopoietic reconstitution with intramarrow injected tall/scl cells because of early lung tumor production and death. Then we tried to find out second gene which may assist the hematopoietic differentiation of tall/scl cells in vivo. For this purpose, we constructed human fetal liver cDNA expression VSV-G pseudotyped lentiviral vector library. This library consisted of more than 8x10^7 individual clones with average cDNA length of 2.1kb. DNA sequencing analysis of several fractions showed 60% of them contained full length cDNAs. When we produced virus using the library with 293T cells, 100% of cells demonstrated gene transduction. Average length of the gene transduced cDNA was about 1 kb. Flow cytometric analysis with antiglycophorin antibody showed the gene transduced cells expressed glycophorin, which is known to be one of the highly abundantly expressed genes in human fetal liver, properly. Using this novel human fetal liver cDNA library, we have already cloned several candidate genes which promotes hematopoiesis of tall/scl cells or induces cytokine independency of cytokine dependent leukemia cell lines. By the identification of genes which differentiate ES cells efficiently to hematopoietic cells in vitro and in vivo, safer and more effective cell supplementation therapy would be realized in future.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (30 results)

All 2006 2005

All Journal Article (24 results) Book (3 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Antiangiogenic activity of BAI1 in vivo : implications for gene therapy of human glioblastomas2006

    • Author(s)
      Kang, X., Tani K., et al.
    • Journal Title

      Cancer Gene Ther 13

      Pages: 385-392

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] An adenovirus serotype 5 vector with fibers derived from ovine atadenovirus demonstrates CAR-independent tropism and unique biodistribution in mice2006

    • Author(s)
      Nakayama, M., Tani K., et al.
    • Journal Title

      Virology 350

      Pages: 103-115

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Serial analysis of gene expression in progressing and regressing mouse tumors implicates the involvement of RANTES and TARC in antitumor immune responses.2006

    • Author(s)
      Nakazaki, Y., Tani K., et al.
    • Journal Title

      Mol Ther. 14

      Pages: 599-606

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tall/scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset (Callithrix jacchus) ES cells.2006

    • Author(s)
      Kurita, R., Tani.K., et al.
    • Journal Title

      Stem Cells 24

      Pages: 2014-2022

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nonhuman Primates : Embryonic stem cells and transgenesis.2006

    • Author(s)
      Horn, P.A., Tani, K., et al.
    • Journal Title

      Cloning and Stem Cells 8

      Pages: 124-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Successful immortalization of mesenchymal progenitor cells derived from human placenta and the differentiation abilities of immortalized cells2006

    • Author(s)
      Zang, X., Tani, K., et al.
    • Journal Title

      Biochem Biophys Res Commun 351

      Pages: 853-859

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Antiangiogenic activity of BAI1 in vivo : implications for gene therapy of human glioblastomas,2006

    • Author(s)
      Kang, X., Tani K., et al.
    • Journal Title

      Cancer Gene Ther 13

      Pages: 385-392

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] An adenovirus serotype 5 vector with fibers derived from ovine atadenovirus demonstrates CAR-independent tropism and unique biodistribution in mice,2006

    • Author(s)
      Nakayama, M., Tani K., et al.
    • Journal Title

      Virology 350

      Pages: 103-115

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Serial analysis of gene expression in progressing and regressing mouse tumors implicates the involvement of RANTES and TARC in antitumor immune responses,2006

    • Author(s)
      Nakazaki, Y., Tani, K., et al.
    • Journal Title

      Mol Ther 14

      Pages: 599-606

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tal1/scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset (Callithrix jacchus) ES cells,2006

    • Author(s)
      Kurita, R., Tani K., et al.
    • Journal Title

      Stem Cells 24

      Pages: 2014-2022

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nonhuman Primates : Embryonic stem cells and transgenesis,2006

    • Author(s)
      Horn, P.A., Tani, K., et al.
    • Journal Title

      Cloning and Stem Cells 8

      Pages: 124-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Successful immortalization of mesenchymal progenitor cells derived from human placenta and the differentiation abilities of immortalized cells,2006

    • Author(s)
      Zang, X., Tani, K., et al.
    • Journal Title

      Biochem Biophys Re Commun 351

      Pages: 853-859

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Antiangiogenic activity of BAI1 in vivo : implications for gene therapy of human glioblastomas2006

    • Author(s)
      Kang, X, Tani K. et al.
    • Journal Title

      Cancer Gene Ther 13

      Pages: 385-392

    • Related Report
      2006 Annual Research Report
  • [Journal Article] An adenovirus serotype 5 vector with fibers derived from ovine atadenovirus demonstrates CAR-independent tropism and unique biodistribution in mice2006

    • Author(s)
      Nakayama, M., Tani K.et al.
    • Journal Title

      Virology 350

      Pages: 103-115

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Serial analysis of gene expression in progressing and regressing mouse tumors implicates the involvement of RANTES and TARC in antitumor immune responses.2006

    • Author(s)
      Nakazaki, Y., Tani K.et al.
    • Journal Title

      Mol Ther 14

      Pages: 599-606

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Tal1/scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset (Callithrix jacchus) ES cells.2006

    • Author(s)
      Kurita, R., Tani K.et al.
    • Journal Title

      Stem Cells 24

      Pages: 2014-2022

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nonhuman Primates : Embryonic stem cells and transgenesis.2006

    • Author(s)
      Horn, P.A., Tani K.et al.
    • Journal Title

      Cloning and Stem Cells 8

      Pages: 124-129

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Successful immortalization of mesenchymal progenitor cells derived from human placenta and the differentiation abilities of immortalized cells2006

    • Author(s)
      Zang, X., Tani K.et al.
    • Journal Title

      Biochem Biophys Res Commun 351

      Pages: 853-859

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Rb plays a role in survival of Abl-dependent human tumor cells as a downstream effector of Abl tyrosine kinase2006

    • Author(s)
      Nagano.K, Tani, K.et al.
    • Journal Title

      Oncogene 25

      Pages: 493-502

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Antiangiogenic activity of BAI1 in vivo : implications for gene therapy of human glioblastomas2006

    • Author(s)
      Kang, X., Tani, K.et al.
    • Journal Title

      Cancer Gene Ther (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] An adenovirus serotype 5 vector with fibers derived from ovine atadenovirus demonstrates CAR-independent tropism and unique biodistribution in mice2006

    • Author(s)
      Nakayama, M., Tani, K.et al.
    • Journal Title

      J Virol (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Sustained molecular remission by non-myeloablative stem cell transplantation after autologous hematopoietic stem cell transplantation in a patient with multiple myeloma.2005

    • Author(s)
      Nakashima, Y., Tani, K., et al.
    • Journal Title

      Leuk Lynphoma 46

      Pages: 1217-1222

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Establishment of Novel Embryonic Stem Cell Lines Derived from the Common Marmoset (Callithrix jacchus)2005

    • Author(s)
      Sasaki, E., Tani, K.et al.
    • Journal Title

      Stem Cells 23

      Pages: 1304-1313

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A novel role of CD30/CD30L signaling in the generation of long-lived memory CD8+T cells2005

    • Author(s)
      Nishimura, H., Tani, K.et al.
    • Journal Title

      J Immunol 175

      Pages: 4627-4634

    • Related Report
      2005 Annual Research Report
  • [Book] 最新医学・第61巻・第6号がん領域におけるドラッグデリバリーシステム(DDS)DDSと細胞療法2006

    • Author(s)
      中村 貴文, 谷 憲三朗
    • Publisher
      最新医学社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 最新医学・第61巻・第6号 がん領域におけるドラッグデリバリーシステム(DDS) DDS と細胞療法2006

    • Author(s)
      中村 貴文, 谷 憲三朗
    • Publisher
      最新医学社
    • Related Report
      2006 Annual Research Report
  • [Book] 遺伝子診療学2005

    • Author(s)
      谷 憲三朗
    • Publisher
      日本臨床社
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] Methods for acceleration of stem cell differentiation2006

    • Inventor(s)
      Tani, K. et al.
    • Industrial Property Rights Holder
      Tani, K. et al.
    • Filing Date
      2006-03-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] Methods for acceleration of stem cell differentiation2006

    • Inventor(s)
      Tani, K. et al.
    • Industrial Property Rights Holder
      Tani, K. et al.
    • Filing Date
      2006-03-15
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] New US Providional Patent application2005

    • Inventor(s)
      Tani, K., Kurita, R
    • Industrial Property Rights Holder
      Tani, K., Kurita, R
    • Filing Date
      2005-03-15
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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